Strain identifier

BacDive ID: 14338

Type strain: Yes

Species: Spirochaeta dissipatitropha

Strain Designation: ASpC2

Strain history: E. V. Pikuta ASpC2.

NCBI tax ID(s): 324679 (species)

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General

@ref: 17297

BacDive-ID: 14338

DSM-Number: 23605

keywords: 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative, motile

description: Spirochaeta dissipatitropha ASpC2 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from Anaerobic sediment of alkaline, hypersaline lake.

NCBI tax id

  • NCBI tax id: 324679
  • Matching level: species

strain history

@refhistory
17297<- JCM <- E. V. Pikuta; ASpC2
67770E. V. Pikuta ASpC2.

doi: 10.13145/bacdive14338.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/spirochaetota
  • domain: Bacteria
  • phylum: Spirochaetota
  • class: Spirochaetia
  • order: Spirochaetales
  • family: Spirochaetaceae
  • genus: Spirochaeta
  • species: Spirochaeta dissipatitropha
  • full scientific name: Spirochaeta dissipatitropha Pikuta et al. 2009

@ref: 17297

domain: Bacteria

phylum: Spirochaetes

class: Spirochaetia

order: Spirochaetales

family: Spirochaetaceae

genus: Spirochaeta

species: Spirochaeta dissipatitropha

full scientific name: Spirochaeta dissipatitropha Pikuta et al. 2009

strain designation: ASpC2

type strain: yes

Morphology

cell morphology

  • @ref: 29598
  • gram stain: negative
  • cell length: 8.0-18.0 µm
  • cell width: 0.23 µm
  • motility: yes

Culture and growth conditions

culture medium

  • @ref: 17297
  • name: SPIROCHAETA DISSIPATITROPHA MEDIUM (DSMZ Medium 1263)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1263
  • composition: Name: SPIROCHAETA DISSIPATITROPHA MEDIUM (DSMZ Medium 1263) Composition: NaHCO3 24.0 g/l Cellobiose 5.0 g/l Na2CO3 2.76 g/l NH4Cl 1.0 g/l Yeast extract 0.5 g/l Na2S x 9 H2O 0.4 g/l K2HPO4 0.2 g/l KCl 0.2 g/l MgCl2 x 6 H2O 0.1 g/l MgSO4 x 7 H2O 0.03 g/l Nitrilotriacetic acid 0.015 g/l NaCl 0.01 g/l MnSO4 x H2O 0.005 g/l CoSO4 x 7 H2O 0.0018 g/l ZnSO4 x 7 H2O 0.0018 g/l FeSO4 x 7 H2O 0.001 g/l CaCl2 x 2 H2O 0.001 g/l Sodium resazurin 0.0005 g/l NiCl2 x 6 H2O 0.0003 g/l AlK(SO4)2 x 12 H2O 0.0002 g/l H3BO3 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l CuSO4 x 5 H2O 0.0001 g/l Pyridoxine hydrochloride 2e-05 g/l Riboflavin 1e-05 g/l (DL)-alpha-Lipoic acid 1e-05 g/l p-Aminobenzoic acid 1e-05 g/l Calcium D-(+)-pantothenate 1e-05 g/l Nicotinic acid 1e-05 g/l Thiamine HCl 1e-05 g/l Folic acid 4e-06 g/l Biotin 4e-06 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l Vitamin B12 2e-07 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
17297positivegrowth30mesophilic
29598positivegrowth13-41
29598positiveoptimum35mesophilic
67770positivegrowth35mesophilic

culture pH

@refabilitytypepHPH range
29598positivegrowth7.8-10.5alkaliphile
29598positiveoptimum10

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
17297anaerobe
29598anaerobe

halophily

@refsaltgrowthtested relationconcentration
29598NaClpositivegrowth01-03 %
29598NaClpositiveoptimum2 %

observation

  • @ref: 29598
  • observation: aggregates in clumps

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2959830089acetate+carbon source
2959816449alanine+carbon source
2959822599arabinose+carbon source
2959829016arginine+carbon source
2959835391aspartate+carbon source
2959816947citrate+carbon source
2959816236ethanol+carbon source
2959828757fructose+carbon source
2959817234glucose+carbon source
2959827570histidine+carbon source
2959817306maltose+carbon source
2959829864mannitol+carbon source
2959817272propionate+carbon source
2959833942ribose+carbon source
2959817992sucrose+carbon source
2959826986threonine+carbon source
2959827082trehalose+carbon source
2959816296D-tryptophan+carbon source

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
17297Anaerobic sediment of alkaline, hypersaline lakeOwen Lake, east-central CaliforniaUSAUSANorth America
67770Anaerobic sediment of alkaline, hypersaline Owen LakeCAUSAUSANorth America

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Sediment
#Condition#Alkaline
#Condition#Anoxic (anaerobic)
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_157433.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_16673;96_2649;97_76879;98_103756;99_157433&stattab=map
  • Last taxonomy: Spirochaeta
  • 16S sequence: AY995150
  • Sequence Identity:
  • Total samples: 160
  • soil counts: 17
  • aquatic counts: 139
  • animal counts: 3
  • plant counts: 1

Safety information

risk assessment

  • @ref: 17297
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 17297
  • description: Spirochaeta dissipatitropha strain ASpC2 16S ribosomal RNA gene, partial sequence
  • accession: AY995150
  • length: 1459
  • database: ena
  • NCBI tax ID: 324679

GC content

@refGC-contentmethod
1729743.8high performance liquid chromatography (HPLC)
2959843.8

External links

@ref: 17297

culture collection no.: DSM 23605, ATCC BAA 1083, JCM 12856

straininfo link

  • @ref: 83483
  • straininfo: 298230

literature

  • topic: Phylogeny
  • Pubmed-ID: 19578151
  • title: Spirochaeta dissipatitropha sp. nov., an alkaliphilic, obligately anaerobic bacterium, and emended description of the genus Spirochaeta Ehrenberg 1835.
  • authors: Pikuta EV, Hoover RB, Bej AK, Marsic D, Whitman WB, Krader P
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.65862-0
  • year: 2009
  • mesh: Anaerobiosis, Bacterial Typing Techniques, Base Composition, California, DNA, Bacterial/analysis, Fatty Acids/analysis, Fresh Water/*microbiology, Genes, rRNA, Genotype, Geologic Sediments/*microbiology, Hydrogen-Ion Concentration, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity, Spirochaeta/*classification/genetics/isolation & purification/physiology
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
17297Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23605)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23605
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29598Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2598928776041
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
83483Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID298230.1StrainInfo: A central database for resolving microbial strain identifiers