Strain identifier

BacDive ID: 14335

Type strain: Yes

Species: Alkalispirochaeta americana

Strain Designation: ASpG1

Strain history: <- E. V. Pikuta, Center Space Plasma and Aeronomic Res. (CSPAR), Univ. Alabama Huntsville, USA; ASpG1

NCBI tax ID(s): 159291 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 5589

BacDive-ID: 14335

DSM-Number: 14872

keywords: genome sequence, 16S sequence, Bacteria, obligate anaerobe, mesophilic, Gram-negative

description: Alkalispirochaeta americana ASpG1 is an obligate anaerobe, mesophilic, Gram-negative bacterium that was isolated from mud sediment of an alkaline, hypersaline, meromictic soda lake.

NCBI tax id

  • NCBI tax id: 159291
  • Matching level: species

strain history

  • @ref: 5589
  • history: <- E. V. Pikuta, Center Space Plasma and Aeronomic Res. (CSPAR), Univ. Alabama Huntsville, USA; ASpG1

doi: 10.13145/bacdive14335.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/spirochaetota
  • domain: Bacteria
  • phylum: Spirochaetota
  • class: Spirochaetia
  • order: Spirochaetales
  • family: Spirochaetaceae
  • genus: Alkalispirochaeta
  • species: Alkalispirochaeta americana
  • full scientific name: Alkalispirochaeta americana (Hoover et al. 2003) Sravanthi et al. 2016
  • synonyms

    • @ref: 20215
    • synonym: Spirochaeta americana

@ref: 5589

domain: Bacteria

phylum: Spirochaetes

class: Spirochaetia

order: Spirochaetales

family: Spirochaetaceae

genus: Alkalispirochaeta

species: Alkalispirochaeta americana

full scientific name: Alkalispirochaeta americana (Hoover et al. 2003) Sravanthi et al. 2016

strain designation: ASpG1

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: negative
  • confidence: 99.945

Culture and growth conditions

culture medium

  • @ref: 5589
  • name: SPIROCHAETA AMERICANA MEDIUM (DSMZ Medium 1165)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1165
  • composition: Name: SPIROCHAETA AMERICANA MEDIUM (DSMZ Medium 1165) Composition: NaCl 30.0 g/l NaHCO3 24.0 g/l D-Glucose 5.0 g/l Na2CO3 2.76 g/l NH4Cl 1.0 g/l Yeast extract 0.5 g/l Na2S x 9 H2O 0.4 g/l KCl 0.2 g/l K2HPO4 0.2 g/l MgCl2 x 6 H2O 0.1 g/l MnCl2 x 4 H2O 0.0036 g/l Fe(NH4)2(SO4)2 x 12 H2O 0.002 g/l ZnSO4 x 7 H2O 0.001 g/l CoCl2 x 6 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Resazurin 0.001 g/l NiCl2 0.0005 g/l H3BO3 0.0001 g/l CuSO4 x 5 H2O 0.0001 g/l AlK(SO4)2 x 12 H2O 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l Pyridoxine hydrochloride 2e-05 g/l Lipoic acid 1e-05 g/l p-Aminobenzoic acid 1e-05 g/l D-Calcium pantothenate 1e-05 g/l Nicotinic acid 1e-05 g/l Riboflavin 1e-05 g/l Thiamine-HCl x 2 H2O 1e-05 g/l Folic acid 4e-06 g/l Biotin 4e-06 g/l Vitamin B12 2e-07 g/l HCl Distilled water

culture temp

  • @ref: 5589
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
5589obligate anaerobe
69480anaerobe99.086

spore formation

@refspore formationconfidence
69481no100
69480no99.984

Isolation, sampling and environmental information

isolation

  • @ref: 5589
  • sample type: mud sediment of an alkaline, hypersaline, meromictic soda lake
  • geographic location: northern California, Mono Lake
  • country: USA
  • origin.country: USA
  • continent: North America

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Mud (Sludge)
#Environmental#Aquatic#Sediment
#Condition#Alkaline
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_7454.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_642;96_3481;97_4268;98_5462;99_7454&stattab=map
  • Last taxonomy: Alkalispirochaeta
  • 16S sequence: AF373921
  • Sequence Identity:
  • Total samples: 382
  • soil counts: 26
  • aquatic counts: 304
  • animal counts: 52

Safety information

risk assessment

  • @ref: 5589
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 5589
  • description: Spirochaeta americana ASpG1 16S ribosomal RNA gene, partial sequence
  • accession: AF373921
  • length: 1467
  • database: ena
  • NCBI tax ID: 159291

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Alkalispirochaeta americana ASpG1GCA_900156105scaffoldncbi159291
66792Alkalispirochaeta americana ASpG12706795025draftimg159291

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes86.79no
flagellatedno71.564no
gram-positiveno96.239no
anaerobicyes97.041yes
aerobicno97.816yes
halophileyes52.703no
spore-formingno91.083no
thermophileno71.915no
glucose-utilyes89.597no
glucose-fermentyes66.623no

External links

@ref: 5589

culture collection no.: DSM 14872, ATCC BAA 392

straininfo link

  • @ref: 83480
  • straininfo: 100107

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12807206Spirochaeta americana sp. nov., a new haloalkaliphilic, obligately anaerobic spirochaete isolated from soda Mono Lake in California.Hoover RB, Pikuta EV, Bej AK, Marsic D, Whitman WB, Tang J, Krader PInt J Syst Evol Microbiol10.1099/ijs.0.02535-02003Anaerobiosis, Bacterial Typing Techniques, Base Composition, California, Culture Media, DNA, Ribosomal/analysis, Hydrogen-Ion Concentration, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/*metabolism, Spirochaeta/*classification/genetics/*growth & development/isolation & purification/metabolism, *Water MicrobiologyCultivation
Phylogeny23148099Spirochaeta sphaeroplastigenens sp. nov., a halo-alkaliphilic, obligately anaerobic spirochaete isolated from soda lake Lonar.Vishnuvardhan Reddy S, Aspana S, Tushar DL, Sasikala C, Ramana CVInt J Syst Evol Microbiol10.1099/ijs.0.046292-02012Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, India, Lakes/microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spirochaeta/*classification/genetics/isolation & purification, *Water MicrobiologyGenetics
Phylogeny26377069Spirochaeta odontotermitis sp. nov., an obligately anaerobic, cellulolytic, halotolerant, alkaliphilic spirochaete isolated from the termite Odontotermes obesus (Rambur) gut.Sravanthi T, Tushar L, Sasikala C, Ramana CVInt J Syst Evol Microbiol10.1099/ijsem.0.0006162015Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, India, Isoptera/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spirochaeta/*classification/genetics/isolation & purificationGenetics
Phylogeny26704619Alkalispirochaeta cellulosivorans gen. nov., sp. nov., a cellulose-hydrolysing, alkaliphilic, halotolerant bacterium isolated from the gut of a wood-eating cockroach (Cryptocercus punctulatus), and reclassification of four species of Spirochaeta as new combinations within Alkalispirochaeta gen. nov.Sravanthi T, Tushar L, Sasikala C, Ramana CVInt J Syst Evol Microbiol10.1099/ijsem.0.0008652015Animals, Bacterial Typing Techniques, Base Composition, Cellulose/metabolism, Cockroaches/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, India, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spirochaeta/*classification/genetics/isolation & purification, WoodGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5589Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14872)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14872
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
83480Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID100107.1StrainInfo: A central database for resolving microbial strain identifiers