Strain identifier
BacDive ID: 14319
Type strain:
Species: Oceanispirochaeta litoralis
Strain Designation: R1
Strain history: <- E. Canale-Parola; R1 <- R. B. Hespell and E. Canale-Parola {1969}
NCBI tax ID(s): 151 (species)
General
@ref: 962
BacDive-ID: 14319
DSM-Number: 2029
keywords: 16S sequence, Bacteria, anaerobe, chemolithoautotroph, mesophilic, Gram-negative, motile, helical-shaped
description: Oceanispirochaeta litoralis R1 is an anaerobe, chemolithoautotroph, mesophilic bacterium that was isolated from marine mud.
NCBI tax id
- NCBI tax id: 151
- Matching level: species
strain history
- @ref: 962
- history: <- E. Canale-Parola; R1 <- R. B. Hespell and E. Canale-Parola {1969}
doi: 10.13145/bacdive14319.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/spirochaetota
- domain: Bacteria
- phylum: Spirochaetota
- class: Spirochaetia
- order: Spirochaetales
- family: Spirochaetaceae
- genus: Oceanispirochaeta
- species: Oceanispirochaeta litoralis
- full scientific name: Oceanispirochaeta litoralis (Canale-Parola 1980 ex Hespell and Canale-Parola 1970) Subhash and Lee 2017
synonyms
- @ref: 20215
- synonym: Spirochaeta litoralis
@ref: 962
domain: Bacteria
phylum: Spirochaetes
class: Spirochaetia
order: Spirochaetales
family: Spirochaetaceae
genus: Oceanispirochaeta
species: Oceanispirochaeta litoralis
full scientific name: Oceanispirochaeta litoralis (Canale-Parola 1980) Subhash and Lee 2017
strain designation: R1
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility |
---|---|---|---|---|---|
43495 | negative | 6-7 µm | 0.2-0.3 µm | helical-shaped | yes |
43496 | negative | 0.8-1.5 µm | 0.5-0.6 µm | rod-shaped | no |
Culture and growth conditions
culture medium
- @ref: 962
- name: OCEANISPIROCHAETA LITORALIS MEDIUM (DSMZ Medium 173)
- growth: yes
- link: https://mediadive.dsmz.de/medium/173
- composition: Name: OCEANISPIROCHAETA LITORALIS MEDIUM (DSMZ Medium 173) Composition: D-Glucose 2.0 g/l Yeast extract 1.0 g/l Trypticase peptone 1.0 g/l L-Cysteine HCl x H2O 0.5 g/l Sodium resazurin 0.0005 g/l Sea water Tris-HCl buffer Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
962 | positive | growth | 30 | mesophilic |
43495 | positive | growth | 15-38 | |
43495 | positive | optimum | 30-32 | mesophilic |
43496 | positive | growth | 47-75 | thermophilic |
43496 | positive | optimum | 65 | thermophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
43495 | positive | growth | 6.5-9 | alkaliphile |
43495 | positive | optimum | 7-7.5 | |
43496 | positive | growth | 5.5-7.5 | |
43496 | positive | optimum | 6.5 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
962 | anaerobe |
43495 | obligate anaerobe |
43496 | anaerobe |
nutrition type
- @ref: 43496
- type: chemolithoautotroph
spore formation
- @ref: 43496
- type of spore: endospore
- spore formation: no
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
43495 | NaCl | positive | growth | 0.5-6.5 % |
43495 | NaCl | positive | optimum | 1.5-2 % |
43496 | NaCl | positive | growth | 1.5-4.5 %(w/v) |
43496 | NaCl | positive | optimum | 2-2.5 %(w/v) |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
43496 | 22599 | arabinose | - | fermentation |
43496 | 28757 | fructose | - | fermentation |
43496 | 17234 | glucose | - | fermentation |
43496 | 17306 | maltose | - | fermentation |
43496 | peptone | - | fermentation | |
43496 | 15361 | pyruvate | - | fermentation |
43496 | 17992 | sucrose | - | fermentation |
43495 | 17924 | D-sorbitol | - | growth |
43495 | 29985 | L-glutamate | - | growth |
43495 | 62345 | L-rhamnose | - | growth |
43495 | 30031 | succinate | - | growth |
43496 | 29034 | ferric iron | - | reduction |
43496 | 16189 | sulfate | - | reduction |
43495 | 29806 | fumarate | + | growth |
43495 | 30849 | L-arabinose | + | growth |
43495 | 29991 | L-aspartate | + | growth |
43495 | 15361 | pyruvate | + | growth |
43495 | 16634 | raffinose | + | growth |
43496 | 17632 | nitrate | + | reduction |
enzymes
@ref | value | activity | ec |
---|---|---|---|
43495 | catalase | - | 1.11.1.6 |
43495 | cytochrome oxidase | - | 1.9.3.1 |
43495 | caseinase | - | 3.4.21.50 |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | latitude | longitude | isolation date |
---|---|---|---|---|---|---|---|---|
962 | marine mud | San Juan, Luquillo Beach Sandbar | Puerto Rico | PRI | Middle and South America | |||
43496 | deep-sea hydrothermal sulfidic vent chimney located on the Eastern Lau Spreading Centre at the depth of 1870m | South-western Pacific Ocean, Tui Malila hydrothermal field | -21.989 | -176.567 | 2009-06 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Condition | #Sulfuric | |
#Condition | #Thermophilic (>45°C) | |
#Environmental | #Aquatic | #Hydrothermal vent |
#Environmental | #Aquatic | #Marine |
taxonmaps
- @ref: 69479
- File name: preview.99_117932.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_1926;96_46657;97_59914;98_79603;99_117932&stattab=map
- Last taxonomy: Oceanispirochaeta litoralis subclade
- 16S sequence: FR733665
- Sequence Identity:
- Total samples: 617
- soil counts: 21
- aquatic counts: 565
- animal counts: 27
- plant counts: 4
Safety information
risk assessment
- @ref: 962
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 962
- description: Spirochaeta litoralis partial 16S rRNA gene, type strain DSM2029T
- accession: FR733665
- length: 1533
- database: ena
- NCBI tax ID: 151
GC content
- @ref: 43496
- GC-content: 49.6
- method: thermal denaturation, midpoint method (Tm)
External links
@ref: 962
culture collection no.: DSM 2029, ATCC 27000
straininfo link
- @ref: 83469
- straininfo: 38745
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
962 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2029) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-2029 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
43495 | Y. Subhash, Sang-Seob Lee | 10.1099/ijsem.0.002130 | Description of Oceanispirochaeta sediminicola gen. nov., sp. nov., an obligately anaerobic bacterium isolated from coastal marine sediments, and reclassification of Spirochaeta litoralis as Oceanispirochaeta litoralis comb. nov. | IJSEM 67: 3403-3409 2017 | 28875893 | |
43496 | Galina B. Slobodkina, Anna-Louise Reysenbach, Tatyana V. Kolganova, Andrei A. Novikov, Elizaveta A. Bonch-Osmolovskaya, Alexander I. Slobodkin | 10.1099/ijsem.0.002142 | Thermosulfuriphilus ammonigenes gen. nov., sp. nov., a thermophilic, chemolithoautotrophic bacterium capable of respiratory ammonification of nitrate with elemental sulfur | IJSEM 67: 3474-3479 2017 | 28857038 | |
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
83469 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID38745.1 | StrainInfo: A central database for resolving microbial strain identifiers |