Strain identifier

BacDive ID: 14319

Type strain: Yes

Species: Oceanispirochaeta litoralis

Strain Designation: R1

Strain history: <- E. Canale-Parola; R1 <- R. B. Hespell and E. Canale-Parola {1969}

NCBI tax ID(s): 151 (species)

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General

@ref: 962

BacDive-ID: 14319

DSM-Number: 2029

keywords: 16S sequence, Bacteria, anaerobe, chemolithoautotroph, mesophilic, Gram-negative, motile, helical-shaped

description: Oceanispirochaeta litoralis R1 is an anaerobe, chemolithoautotroph, mesophilic bacterium that was isolated from marine mud.

NCBI tax id

  • NCBI tax id: 151
  • Matching level: species

strain history

  • @ref: 962
  • history: <- E. Canale-Parola; R1 <- R. B. Hespell and E. Canale-Parola {1969}

doi: 10.13145/bacdive14319.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/spirochaetota
  • domain: Bacteria
  • phylum: Spirochaetota
  • class: Spirochaetia
  • order: Spirochaetales
  • family: Spirochaetaceae
  • genus: Oceanispirochaeta
  • species: Oceanispirochaeta litoralis
  • full scientific name: Oceanispirochaeta litoralis (Canale-Parola 1980 ex Hespell and Canale-Parola 1970) Subhash and Lee 2017

  • synonyms

    • @ref: 20215
    • synonym: Spirochaeta litoralis

@ref: 962

domain: Bacteria

phylum: Spirochaetes

class: Spirochaetia

order: Spirochaetales

family: Spirochaetaceae

genus: Oceanispirochaeta

species: Oceanispirochaeta litoralis

full scientific name: Oceanispirochaeta litoralis (Canale-Parola 1980) Subhash and Lee 2017

strain designation: R1

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
43495negative6-7 µm0.2-0.3 µmhelical-shapedyes
43496negative0.8-1.5 µm0.5-0.6 µmrod-shapedno

Culture and growth conditions

culture medium

  • @ref: 962
  • name: OCEANISPIROCHAETA LITORALIS MEDIUM (DSMZ Medium 173)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/173
  • composition: Name: OCEANISPIROCHAETA LITORALIS MEDIUM (DSMZ Medium 173) Composition: D-Glucose 2.0 g/l Yeast extract 1.0 g/l Trypticase peptone 1.0 g/l L-Cysteine HCl x H2O 0.5 g/l Sodium resazurin 0.0005 g/l Sea water Tris-HCl buffer Distilled water

culture temp

@refgrowthtypetemperaturerange
962positivegrowth30mesophilic
43495positivegrowth15-38
43495positiveoptimum30-32mesophilic
43496positivegrowth47-75thermophilic
43496positiveoptimum65thermophilic

culture pH

@refabilitytypepHPH range
43495positivegrowth6.5-9alkaliphile
43495positiveoptimum7-7.5
43496positivegrowth5.5-7.5
43496positiveoptimum6.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
962anaerobe
43495obligate anaerobe
43496anaerobe

nutrition type

  • @ref: 43496
  • type: chemolithoautotroph

spore formation

  • @ref: 43496
  • type of spore: endospore
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
43495NaClpositivegrowth0.5-6.5 %
43495NaClpositiveoptimum1.5-2 %
43496NaClpositivegrowth1.5-4.5 %(w/v)
43496NaClpositiveoptimum2-2.5 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4349622599arabinose-fermentation
4349628757fructose-fermentation
4349617234glucose-fermentation
4349617306maltose-fermentation
43496peptone-fermentation
4349615361pyruvate-fermentation
4349617992sucrose-fermentation
4349517924D-sorbitol-growth
4349529985L-glutamate-growth
4349562345L-rhamnose-growth
4349530031succinate-growth
4349629034ferric iron-reduction
4349616189sulfate-reduction
4349529806fumarate+growth
4349530849L-arabinose+growth
4349529991L-aspartate+growth
4349515361pyruvate+growth
4349516634raffinose+growth
4349617632nitrate+reduction

enzymes

@refvalueactivityec
43495catalase-1.11.1.6
43495cytochrome oxidase-1.9.3.1
43495caseinase-3.4.21.50

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeisolation date
962marine mudSan Juan, Luquillo Beach SandbarPuerto RicoPRIMiddle and South America
43496deep-sea hydrothermal sulfidic vent chimney located on the Eastern Lau Spreading Centre at the depth of 1870mSouth-western Pacific Ocean, Tui Malila hydrothermal field-21.989-176.5672009-06

isolation source categories

Cat1Cat2Cat3
#Condition#Sulfuric
#Condition#Thermophilic (>45°C)
#Environmental#Aquatic#Hydrothermal vent
#Environmental#Aquatic#Marine

taxonmaps

  • @ref: 69479
  • File name: preview.99_117932.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_1926;96_46657;97_59914;98_79603;99_117932&stattab=map
  • Last taxonomy: Oceanispirochaeta litoralis subclade
  • 16S sequence: FR733665
  • Sequence Identity:
  • Total samples: 617
  • soil counts: 21
  • aquatic counts: 565
  • animal counts: 27
  • plant counts: 4

Safety information

risk assessment

  • @ref: 962
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 962
  • description: Spirochaeta litoralis partial 16S rRNA gene, type strain DSM2029T
  • accession: FR733665
  • length: 1533
  • database: ena
  • NCBI tax ID: 151

GC content

  • @ref: 43496
  • GC-content: 49.6
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 962

culture collection no.: DSM 2029, ATCC 27000

straininfo link

  • @ref: 83469
  • straininfo: 38745

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
962Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2029)https://www.dsmz.de/collection/catalogue/details/culture/DSM-2029
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
43495Y. Subhash, Sang-Seob Lee10.1099/ijsem.0.002130Description of Oceanispirochaeta sediminicola gen. nov., sp. nov., an obligately anaerobic bacterium isolated from coastal marine sediments, and reclassification of Spirochaeta litoralis as Oceanispirochaeta litoralis comb. nov.IJSEM 67: 3403-3409 201728875893
43496Galina B. Slobodkina, Anna-Louise Reysenbach, Tatyana V. Kolganova, Andrei A. Novikov, Elizaveta A. Bonch-Osmolovskaya, Alexander I. Slobodkin10.1099/ijsem.0.002142Thermosulfuriphilus ammonigenes gen. nov., sp. nov., a thermophilic, chemolithoautotrophic bacterium capable of respiratory ammonification of nitrate with elemental sulfurIJSEM 67: 3474-3479 201728857038
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
83469Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID38745.1StrainInfo: A central database for resolving microbial strain identifiers