Strain identifier
BacDive ID: 14318
Type strain:
Species: Spirochaeta isovalerica
Strain Designation: MA-2
Strain history: <- C. S. Harwood; MA-2 <- C. S. Harwood {1974}
NCBI tax ID(s): 150 (species)
General
@ref: 1086
BacDive-ID: 14318
DSM-Number: 2461
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative
description: Spirochaeta isovalerica MA-2 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from marine intertidal mud.
NCBI tax id
- NCBI tax id: 150
- Matching level: species
strain history
- @ref: 1086
- history: <- C. S. Harwood; MA-2 <- C. S. Harwood {1974}
doi: 10.13145/bacdive14318.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/spirochaetota
- domain: Bacteria
- phylum: Spirochaetota
- class: Spirochaetia
- order: Spirochaetales
- family: Spirochaetaceae
- genus: Spirochaeta
- species: Spirochaeta isovalerica
- full scientific name: Spirochaeta isovalerica Harwood and Canale-Parola 1983
@ref: 1086
domain: Bacteria
phylum: Spirochaetes
class: Spirochaetia
order: Spirochaetales
family: Spirochaetaceae
genus: Spirochaeta
species: Spirochaeta isovalerica
full scientific name: Spirochaeta isovalerica Harwood and Canale-Parola 1983
strain designation: MA-2
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: negative
- confidence: 99.807
Culture and growth conditions
culture medium
- @ref: 1086
- name: OCEANISPIROCHAETA LITORALIS MEDIUM (DSMZ Medium 173)
- growth: yes
- link: https://mediadive.dsmz.de/medium/173
- composition: Name: OCEANISPIROCHAETA LITORALIS MEDIUM (DSMZ Medium 173) Composition: D-Glucose 2.0 g/l Yeast extract 1.0 g/l Trypticase peptone 1.0 g/l L-Cysteine HCl x H2O 0.5 g/l Sodium resazurin 0.0005 g/l Sea water Tris-HCl buffer Distilled water
culture temp
- @ref: 1086
- growth: positive
- type: growth
- temperature: 30
- range: mesophilic
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance | confidence |
---|---|---|
1086 | anaerobe | |
69480 | anaerobe | 100 |
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 100 |
69480 | no | 99.946 |
Isolation, sampling and environmental information
isolation
- @ref: 1086
- sample type: marine intertidal mud
- geographic location: Massachusetts, Woods Hole
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Marine |
#Environmental | #Terrestrial | #Mud (Sludge) |
#Environmental | #Terrestrial | #Tidal flat |
Safety information
risk assessment
- @ref: 1086
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Spirochaeta isovalerica partial 16S rRNA gene, type strain DSM 2461T, clone 1 | FR749930 | 1533 | ena | 150 |
20218 | Spirochaeta isovalerica 16S ribosomal RNA | M88720 | 1569 | ena | 150 |
1086 | Spirochaeta isovalerica partial 16S rRNA gene, type strain DSM 2461T, clone 2 | FR749931 | 1534 | ena | 150 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Spirochaeta isovalerica DSM 2461 | GCA_014207565 | scaffold | ncbi | 150 |
66792 | Spirochaeta isovalerica strain DSM 2461 | 150.10 | wgs | patric | 150 |
66792 | Spirochaeta isovalerica DSM 2461 | 2861426075 | draft | img | 150 |
GC content
- @ref: 1086
- GC-content: 64.5
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
flagellated | no | 76.841 | no |
gram-positive | no | 94.357 | no |
anaerobic | yes | 95.497 | no |
aerobic | no | 95.958 | yes |
halophile | no | 81.663 | no |
spore-forming | no | 80.874 | no |
glucose-util | yes | 88.072 | no |
thermophile | no | 85.428 | yes |
motile | yes | 75.949 | no |
glucose-ferment | yes | 56.749 | no |
External links
@ref: 1086
culture collection no.: DSM 2461, ATCC 33939
straininfo link
- @ref: 83468
- straininfo: 46771
literature
- topic: Phylogeny
- Pubmed-ID: 24860115
- title: Spirochaeta psychrophila sp. nov., a psychrophilic spirochaete isolated from subseafloor sediment, and emended description of the genus Spirochaeta.
- authors: Miyazaki M, Sakai S, Yamanaka Y, Saito Y, Takai K, Imachi H
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.062463-0
- year: 2014
- mesh: Bacterial Typing Techniques, Base Composition, Bioreactors/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Japan, Molecular Sequence Data, Phospholipids/chemistry, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/microbiology, Sequence Analysis, DNA, Spirochaeta/*classification/genetics/isolation & purification
- topic2: Genetics
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
1086 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2461) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-2461 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
83468 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID46771.1 | StrainInfo: A central database for resolving microbial strain identifiers |