Strain identifier
BacDive ID: 14311
Type strain: 
Species: Borrelia bissettiae
Strain Designation: DN127, DN 127
Strain history: CIP <- 2006, D. Postic, Inst. Pasteur, Paris, France, Borrelia bissettii: strain DN 127 <- M. Janda <- M. Bissett
NCBI tax ID(s): 64897 (species)
version 8.1 (current version)
General
@ref: 7320
BacDive-ID: 14311
DSM-Number: 17990
keywords: 16S sequence, Bacteria, microaerophile, mesophilic, motile, helical-shaped
description: Borrelia bissettiae DN127 is a microaerophile, mesophilic, motile bacterium that was isolated from tick Ixodes pacificus.
NCBI tax id
- NCBI tax id: 64897
- Matching level: species
strain history
| @ref | history |
|---|---|
| 7320 | <- D. Postic; DN127 {2006} |
| 116823 | CIP <- 2006, D. Postic, Inst. Pasteur, Paris, France, Borrelia bissettii: strain DN 127 <- M. Janda <- M. Bissett |
doi: 10.13145/bacdive14311.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/spirochaetota
- domain: Bacteria
- phylum: Spirochaetota
- class: Spirochaetia
- order: Spirochaetales
- family: Borreliaceae
- genus: Borrelia
- species: Borrelia bissettiae
- full scientific name: Borrelia bissettiae Margos et al. 2016
synonyms
@ref synonym 20215 Borreliella bissettiae 20215 Borrelia bissettii
@ref: 7320
domain: Bacteria
phylum: Spirochaetes
class: Spirochaetia
order: Spirochaetales
family: Borreliaceae
genus: Borrelia
species: Borrelia bissettiae
full scientific name: Borrelia bissettiae Margos et al. 2016
strain designation: DN127, DN 127
type strain: yes
Morphology
cell morphology
- @ref: 43710
- cell length: 20 µm
- cell width: 0.2 µm
- cell shape: helical-shaped
- motility: yes
colony morphology
- @ref: 7320
- incubation period: 3-7 days
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 7320 | BSK-MEDIUM (DSMZ Medium 403) | yes | https://mediadive.dsmz.de/medium/403 | Name: BSK-MEDIUM (DSMZ Medium 403) Composition: Bovine serum albumin 35.0245 g/l Gelatine 9.79706 g/l HEPES buffer 4.19874 g/l Proteose peptone no. 2 3.49895 g/l Glucose 2.09937 g/l Proteose yeastolate 0.69979 g/l Tryptone peptone 0.69979 g/l Na-pyruvate 0.559832 g/l Sodium citrate 0.489853 g/l N-Acetylglucosamine 0.279916 g/l MgCl2 x 6 H2O 0.209937 g/l L-Glutamine 0.069979 g/l Double distilled water CMRL 1066 Rabbit serum |
| 34354 | MEDIUM 83 - for Borrelia | yes | BSK-H medium (1000.000 ml) | |
| 116823 | CIP Medium 83 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=83 |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 7320 | positive | growth | 35 | mesophilic |
| 34354 | positive | growth | 34 | mesophilic |
| 43710 | positive | optimum | 33-34 | mesophilic |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance |
|---|---|
| 7320 | microaerophile |
| 43710 | microaerophile |
Isolation, sampling and environmental information
isolation
| @ref | sample type | host species | geographic location | country | origin.country | continent | isolation date |
|---|---|---|---|---|---|---|---|
| 7320 | tick Ixodes pacificus | Ixodes pacificus | California | USA | USA | North America | |
| 43710 | A questing Ixodes pacificus tick collected in Del Norte County | Del Norte County, California | USA | USA | North America | 1980 | |
| 116823 | Other, Ixodes pacificus | California | United States of America | USA | North America | 1987 |
isolation source categories
- Cat1: #Host
- Cat2: #Arthropoda
- Cat3: #Tick
taxonmaps
- @ref: 69479
- File name: preview.99_427.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15870;96_290;97_320;98_355;99_427&stattab=map
- Last taxonomy: Borreliella
- 16S sequence: AJ224141
- Sequence Identity:
- Total samples: 1677
- soil counts: 126
- aquatic counts: 378
- animal counts: 1133
- plant counts: 40
Safety information
risk assessment
- @ref: 116823
- biosafety level: 2
- biosafety level comment: Risk group (French classification)
Sequence information
16S sequences
- @ref: 7320
- description: Borrelia burgdorferi sensu lato, 16S rRNA gene, isolate DN127
- accession: AJ224141
- length: 1467
- database: ena
- NCBI tax ID: 521010
GC content
- @ref: 7320
- GC-content: 27
External links
@ref: 7320
culture collection no.: DSM 17990, CIP 109136
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 9817861 | Expanded diversity among Californian borrelia isolates and description of Borrelia bissettii sp. nov. (formerly Borrelia group DN127). | Postic D, Ras NM, Lane RS, Hendson M, Baranton G | J Clin Microbiol | 10.1128/JCM.36.12.3497-3504.1998 | 1998 | Borrelia/*classification/genetics, Borrelia burgdorferi Group/*classification, California, Humans, Phylogeny | Pathogenicity |
| Phylogeny | 10921989 | Borrelia isolates in Northern Colorado identified as Borrelia bissettii. | Schneider BS, Zeidner NS, Burkot TR, Maupin GO, Piesman J | J Clin Microbiol | 10.1128/JCM.38.8.3103-3105.2000 | 2000 | Animals, Borrelia/*classification/*genetics/isolation & purification, Borrelia Infections/*veterinary, Colorado, Genes, rRNA, Molecular Sequence Data, Muridae/*microbiology, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal/genetics, Rodent Diseases/*microbiology, Sequence Analysis, DNA | Genetics |
| Phylogeny | 11075925 | Molecular characterization of Borrelia spp. isolates from greater metropolitan Chicago reveals the presence of Borrelia bissettii. Preliminary report. | Picken RN, Picken MM | J Mol Microbiol Biotechnol | 2000 | Animals, Arvicolinae/microbiology, Base Sequence, Borrelia/*classification/*genetics/isolation & purification, Chicago, Disease Reservoirs, Humans, Illinois, *Introns, Lyme Disease/microbiology, Molecular Sequence Data, Rodentia/*microbiology, Sequence Alignment, Sequence Homology, Nucleic Acid, Wisconsin | Pathogenicity | |
| Phylogeny | 14740924 | Bacteriolytic activity of selected vertebrate sera for Borrelia burgdorferi sensu stricto and Borrelia bissettii. | Ullmann AJ, Lane RS, Kurtenbach K, Miller M, Schriefer ME, Zeldner N, Piesman J | J Parasitol | 10.1645/Ge-3081RN | 2003 | Animals, Bacteriolysis/*immunology, Blood Bactericidal Activity/*immunology, Borrelia/*immunology, Borrelia burgdorferi/*immunology, Colinus, Complement System Proteins/*immunology, Deer, Disease Reservoirs, Lizards, Peromyscus, Rabbits, Species Specificity | |
| Phylogeny | 15311469 | Detection and characterization of Borrelia bissettii in rodents from the central California coast. | Vredevoe LK, Stevens JR, Schneider BS | J Med Entomol | 10.1603/0022-2585-41.4.736 | 2004 | Animals, Animals, Wild/microbiology, Borrelia/classification/genetics/*isolation & purification, California, Geography, Phylogeny, Polymerase Chain Reaction, Rodentia/classification/*microbiology | Enzymology |
| Phylogeny | 21157575 | Multilocus sequence analysis of Borrelia bissettii strains from North America reveals a new Borrelia species, Borrelia kurtenbachii. | Margos G, Hojgaard A, Lane RS, Cornet M, Fingerle V, Rudenko N, Ogden N, Aanensen DM, Fish D, Piesman J | Ticks Tick Borne Dis | 10.1016/j.ttbdis.2010.09.002 | 2010 | Animals, Arachnid Vectors/microbiology, Bacterial Typing Techniques, Borrelia/*classification/*genetics, Genes, Bacterial, Genetic Speciation, Ixodes/microbiology, *Multilocus Sequence Typing, North America, *Phylogeny, Species Specificity | Transcriptome |
| Genetics | 22207749 | Whole-genome sequences of Borrelia bissettii, Borrelia valaisiana, and Borrelia spielmanii. | Schutzer SE, Fraser-Liggett CM, Qiu WG, Kraiczy P, Mongodin EF, Dunn JJ, Luft BJ, Casjens SR | J Bacteriol | 10.1128/JB.06263-11 | 2012 | Borrelia burgdorferi Group/*genetics, *Genome, Bacterial, Molecular Sequence Data, Species Specificity | Phylogeny |
| Enzymology | 22367885 | Comparative study of binding of ovine complement factor H with different Borrelia genospecies. | Kisova-Vargova L, Cernanska D, Bhide M | Folia Microbiol (Praha) | 10.1007/s12223-012-0104-y | 2012 | Animals, Bacterial Proteins/chemistry/genetics/immunology, Borrelia/classification/*genetics/*immunology/isolation & purification, Complement Factor H/chemistry/*immunology, Kinetics, Lyme Disease/immunology/microbiology/*veterinary, Molecular Sequence Data, Sheep, Sheep Diseases/*immunology/microbiology | Phylogeny |
| Phylogeny | 26813420 | Borrelia bissettiae sp. nov. and Borrelia californiensis sp. nov. prevail in diverse enzootic transmission cycles. | Margos G, Lane RS, Fedorova N, Koloczek J, Piesman J, Hojgaard A, Sing A, Fingerle V | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000897 | 2016 |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 7320 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17990) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-17990 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 34354 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/6844 | ||||
| 43710 | Gabriele Margos, Robert S. Lane, Natalia Fedorova, Johannes Koloczek, Joseph Piesman, Andrias Hojgaard, Andreas Sing, Volker Fingerle | 10.1099/ijsem.0.000897 | Borrelia bissettiae sp. nov. and Borrelia californiensis sp. nov. prevail in diverse enzootic transmission cycles | IJSEM 66: 1447-1452 2016 | 26813420 | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 116823 | Curators of the CIP | Collection of Institut Pasteur (CIP 109136) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109136 |