Strain identifier
BacDive ID: 14301
Type strain: 
Species: Spirillum winogradskyi
Strain Designation: D-427, D427
Strain history: <- G. A. Dubinina, Russian Academy of Sciences, Inst. of Microbiology, Moscow, Russia; D427 <- M. Yu. Grabovich {1990}
NCBI tax ID(s): 307497 (species)
version 8.1 (current version)
General
@ref: 4807
BacDive-ID: 14301
DSM-Number: 12756
keywords: 16S sequence, Bacteria, microaerophile, mesophilic
description: Spirillum winogradskyi D-427 is a microaerophile, mesophilic bacterium that was isolated from sludge of aeration tank.
NCBI tax id
- NCBI tax id: 307497
- Matching level: species
strain history
- @ref: 4807
- history: <- G. A. Dubinina, Russian Academy of Sciences, Inst. of Microbiology, Moscow, Russia; D427 <- M. Yu. Grabovich {1990}
doi: 10.13145/bacdive14301.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Nitrosomonadales
- family: Spirillaceae
- genus: Spirillum
- species: Spirillum winogradskyi
- full scientific name: Spirillum winogradskyi Podkopaeva et al. 2009
synonyms
- @ref: 20215
- synonym: Spirillum winogradskii
@ref: 4807
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Nitrosomonadales
family: Spirillaceae
genus: Spirillum
species: Spirillum winogradskyi
full scientific name: Spirillum winogradskyi Podkopaeva et al. 2009
strain designation: D-427, D427
type strain: yes
Morphology
colony morphology
- @ref: 4807
- incubation period: 3-7 days
Culture and growth conditions
culture medium
- @ref: 4807
- name: SPIRILLUM D427 MEDIUM (DSMZ Medium 845)
- growth: yes
- link: https://mediadive.dsmz.de/medium/845
- composition: Name: SPIRILLUM WINOGRADSKYI MEDIUM (DSMZ Medium 845) Composition: Peptone 4.98504 g/l (NH4)2SO4 0.997009 g/l Agar 0.997009 g/l MgSO4 x 7 H2O 0.997009 g/l Disodium succinate 0.997009 g/l Na2-EDTA x 2 H2O 0.00518445 g/l FeCl3 x 6 H2O 0.00199402 g/l MnSO4 x H2O 0.00199402 g/l FeCl2 x 4 H2O 0.00149551 g/l Pyridoxine hydrochloride 0.000299103 g/l Thiamine-HCl x 2 H2O 0.000199402 g/l Nicotinic acid 0.000199402 g/l CoCl2 x 6 H2O 0.000189432 g/l Vitamin B12 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l Calcium pantothenate 9.97009e-05 g/l p-Aminobenzoic acid 7.97607e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l D-(+)-biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Catalase Distilled water
culture temp
- @ref: 4807
- growth: positive
- type: growth
- temperature: 28
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 4807
- oxygen tolerance: microaerophile
Isolation, sampling and environmental information
isolation
- @ref: 4807
- sample type: sludge of aeration tank
- geographic location: Voronezh region
- country: Russia
- origin.country: RUS
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Built environment | #Container (Reservoir) |
| #Environmental | #Terrestrial | #Mud (Sludge) |
Safety information
risk assessment
- @ref: 4807
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 4807
- description: Spirillum winogradskyi strain D-427 16S ribosomal RNA gene, partial sequence
- accession: AY845251
- length: 1494
- database: ena
- NCBI tax ID: 307497
GC content
- @ref: 4807
- GC-content: 38
- method: thermal denaturation, midpoint method (Tm)
External links
@ref: 4807
culture collection no.: DSM 12756, VKM B-2518
straininfo link
- @ref: 83454
- straininfo: 49351
literature
| topic | Pubmed-ID | title | authors | journal | year | mesh | topic2 | DOI |
|---|---|---|---|---|---|---|---|---|
| Metabolism | 14679896 | [Oxidative stress and antioxidant cell protection systems in the microaerophilic bacterium Spirillum winogradskii]. | Podkopaeva DA, Grabovich MIu, Dubinina GA | Mikrobiologiia | 2003 | Aerobiosis, Catalase/metabolism, Culture Media, Cytochrome-c Peroxidase/metabolism, Cytoplasm/metabolism, Glutathione Peroxidase/biosynthesis, Glutathione Reductase/biosynthesis, Hydrogen Peroxide/metabolism, *Oxidative Stress, Periplasm/metabolism, Polysaccharides, Bacterial/biosynthesis, Spirillum/growth & development/*metabolism, Thiosulfates | Cultivation | |
| Phylogeny | 16758869 | [Two new species of microaerophilic sulfur spirilla, Spirillum winogradskii sp. nov. and Spirillum kriegii sp. nov]. | Podkopaeva DA, Grabovich MIu, Dubinina GA, Lysenko AM, Turova TP, Kolganova TV | Mikrobiologiia | 2006 | Genotype, Oxygen/pharmacology, Phylogeny, Sewage/*microbiology, Spirillum/isolation & purification/*metabolism/*ultrastructure, Sulfides/metabolism, Sulfur/*metabolism, *Water Microbiology | Metabolism | |
| Phylogeny | 19625415 | Proposal of Spirillum winogradskyi sp. nov., a novel microaerophilic species, an emended description of the genus Spirillum and Request for an Opinion regarding the status of the species Spirillum volutans Ehrenberg 1832. | Podkopaeva D, Grabovich M, Kuever J, Lysenko AM, Tourova TP, Kolganova TV, Dubinina G | Int J Syst Evol Microbiol | 2009 | DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sewage/*microbiology, Spirillum/*classification/genetics/*isolation & purification | Genetics | 10.1099/ijs.0.009605-0 |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 4807 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12756) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-12756 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 83454 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID49351.1 | StrainInfo: A central database for resolving microbial strain identifiers |