Strain identifier

BacDive ID: 14235

Type strain: Yes

Species: Sphingomonas aurantiaca

Strain Designation: MA101b

Strain history: CIP <- 2004, P. Kämpfer, Giessen Univ., Giessen, Germany <- M. Andersson: strain MA101b

NCBI tax ID(s): 185949 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 5443

BacDive-ID: 14235

DSM-Number: 14748

keywords: genome sequence, 16S sequence, Bacteria, psychrophilic, Gram-negative

description: Sphingomonas aurantiaca MA101b is a psychrophilic, Gram-negative bacterium that was isolated from indoor dusts in animal sheds.

NCBI tax id

  • NCBI tax id: 185949
  • Matching level: species

strain history

@refhistory
5443<- H.-J. Busse; MA101b <- M. Andersson
116555CIP <- 2004, P. Kämpfer, Giessen Univ., Giessen, Germany <- M. Andersson: strain MA101b

doi: 10.13145/bacdive14235.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Sphingomonadales
  • family: Sphingomonadaceae
  • genus: Sphingomonas
  • species: Sphingomonas aurantiaca
  • full scientific name: Sphingomonas aurantiaca Busse et al. 2003

@ref: 5443

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Sphingomonadales

family: Sphingomonadaceae

genus: Sphingomonas

species: Sphingomonas aurantiaca

full scientific name: Sphingomonas aurantiaca Busse et al. 2003

strain designation: MA101b

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.886
116555negativerod-shapedyes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5443NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
5443R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
5443YEAST-PEPTONE-SUCCINATE MEDIUM (DSMZ Medium 988)yeshttps://mediadive.dsmz.de/medium/988Name: YEAST-PEPTONE-SUCCINATE MEDIUM (DSMZ Medium 988) Composition: Agar 15.0 g/l Peptone 3.0 g/l Yeast extract 3.0 g/l Sodium succinate 2.3 g/l Distilled water
37291MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromatiyesDistilled water make up to (1000.000 ml);R2A agar (18.200 g)
116555CIP Medium 566yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=566

culture temp

@refgrowthtypetemperaturerange
5443positivegrowth22psychrophilic
37291positivegrowth30mesophilic
116555positivegrowth5-30
116555nogrowth37mesophilic
116555nogrowth41thermophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no94
69480no99.915

halophily

@refsaltgrowthtested relationconcentration
116555NaClpositivegrowth0 %
116555NaClnogrowth2 %
116555NaClnogrowth4 %
116555NaClnogrowth6 %
116555NaClnogrowth8 %
116555NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
116555esculin-hydrolysis4853
116555nitrate-reduction17632
116555nitrite-reduction16301

antibiotic resistance

  • @ref: 116555
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 116555
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
116555oxidase+
116555beta-galactosidase+3.2.1.23
116555alcohol dehydrogenase-1.1.1.1
116555gelatinase-
116555amylase+
116555DNase-
116555caseinase-3.4.21.50
116555catalase+1.11.1.6
116555tween esterase-
116555lecithinase-
116555lipase-
116555lysine decarboxylase-4.1.1.18
116555ornithine decarboxylase-4.1.1.17
116555protease-
116555tryptophan deaminase-
116555urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116555-+++-+----++---++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116555------------------------+/-------------------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
5443indoor dusts in animal shedsFinlandFINEurope
116555Environment, Indoor dusts in animal shedsFinlandFINEurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Livestock (Husbandry)
#Engineered#Built environment#Indoor
#Engineered#Waste#Dust (Ash)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
54431Risk group (German classification)
1165551Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5443
  • description: Sphingomonas aurantiaca partial 16S rRNA gene, strain MA101b
  • accession: AJ429236
  • length: 1461
  • database: ena
  • NCBI tax ID: 185949

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Sphingomonas aurantiaca MA101bGCA_003050705scaffoldncbi185949
66792Sphingomonas aurantiaca MA101b2740891891draftimg185949

GC content

  • @ref: 5443
  • GC-content: 64.7

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno94no
flagellatedno90.497no
gram-positiveno96.963no
anaerobicno99.346no
halophileno94.331no
spore-formingno93.23no
thermophileno99.173yes
glucose-utilyes89.914no
aerobicyes93.464no
motileyes78.186no
glucose-fermentno90.55no

External links

@ref: 5443

culture collection no.: DSM 14748, LMG 21377, CIP 108368

straininfo link

  • @ref: 83394
  • straininfo: 86184

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny13130003Sphingomonas aurantiaca sp. nov., Sphingomonas aerolata sp. nov. and Sphingomonas faeni sp. nov., air- and dustborne and Antarctic, orange-pigmented, psychrotolerant bacteria, and emended description of the genus Sphingomonas.Busse HJ, Denner EBM, Buczolits S, Salkinoja-Salonen M, Bennasar A, Kampfer PInt J Syst Evol Microbiol10.1099/ijs.0.02461-02003Air Microbiology, Antarctic Regions, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Dust, Fatty Acids/analysis, Molecular Sequence Data, Phylogeny, Pigmentation, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity, Sphingomonas/*classification/genetics/isolation & purification/metabolismGenetics
Phylogeny17590230Sphingomonas astaxanthinifaciens sp. nov., a novel astaxanthin-producing bacterium of the family Sphingomonadaceae isolated from Misasa, Tottori, Japan.Asker D, Beppu T, Ueda KFEMS Microbiol Lett10.1111/j.1574-6968.2007.00760.x2007Carotenoids/chemistry/classification, Fresh Water/*microbiology, Japan, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/chemistry/classification, Sequence Analysis, DNA, Sphingomonas/*classification/isolation & purification/metabolism, Xanthophylls/biosynthesisGenetics
Phylogeny23564163Sphingomonas ginsenosidivorax sp. nov., with the ability to transform ginsenosides.Jin XF, Kim JK, Liu QM, Kang MS, He D, Jin FX, Kim SC, Im WTAntonie Van Leeuwenhoek10.1007/s10482-013-9916-22013Bacterial Typing Techniques, Base Composition, Base Sequence, DNA, Bacterial/genetics, Fatty Acids, Fresh Water/*microbiology, Genes, rRNA, Geologic Sediments/*microbiology, Ginsenosides/metabolism, Molecular Sequence Data, Phylogeny, Polyamines/metabolism, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Soil Microbiology, Sphingomonas/*classification/genetics/isolation & purification/*metabolismMetabolism
Phylogeny28792375Sphingomonas olei sp. nov., with the ability to degrade aliphatic hydrocarbons, isolated from oil-contaminated soil.Chaudhary DK, Kim JInt J Syst Evol Microbiol10.1099/ijsem.0.0020102017Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hydrocarbons/metabolism, Nepal, Nucleic Acid Hybridization, *Petroleum Pollution, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Soil Pollutants, Sphingomonas/*classification/genetics/isolation & purification, Ubiquinone/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitle
5443Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14748)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14748
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
37291Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5982
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
83394Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID86184.1StrainInfo: A central database for resolving microbial strain identifiers
116555Curators of the CIPCollection of Institut Pasteur (CIP 108368)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108368