Strain identifier
BacDive ID: 14233
Type strain:
Species: Sphingomonas aerolata
Strain Designation: NW12
Strain history: CIP <- 2004, P. Kämpfer, Giessen Univ., Giessen, Germany <- O. Kim: strain NW12
NCBI tax ID(s): 185951 (species)
General
@ref: 5441
BacDive-ID: 14233
DSM-Number: 14746
keywords: genome sequence, 16S sequence, Bacteria, aerobe, psychrophilic, Gram-negative
description: Sphingomonas aerolata NW12 is an aerobe, psychrophilic, Gram-negative bacterium that was isolated from indoor air of the Sainsburry Center for Visal Art.
NCBI tax id
- NCBI tax id: 185951
- Matching level: species
strain history
@ref | history |
---|---|
5441 | <- H.-J. Busse; NW12 <- O. Kim |
116071 | CIP <- 2004, P. Kämpfer, Giessen Univ., Giessen, Germany <- O. Kim: strain NW12 |
doi: 10.13145/bacdive14233.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Sphingomonadales
- family: Sphingomonadaceae
- genus: Sphingomonas
- species: Sphingomonas aerolata
- full scientific name: Sphingomonas aerolata Busse et al. 2003
@ref: 5441
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Sphingomonadales
family: Sphingomonadaceae
genus: Sphingomonas
species: Sphingomonas aerolata
full scientific name: Sphingomonas aerolata Busse et al. 2003
strain designation: NW12
type strain: yes
Morphology
cell morphology
@ref | gram stain | confidence | cell shape | motility |
---|---|---|---|---|
69480 | negative | 99.835 | ||
116071 | negative | rod-shaped | yes |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
5441 | R2A MEDIUM (DSMZ Medium 830) | yes | https://mediadive.dsmz.de/medium/830 | Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water |
5441 | NUTRIENT AGAR (DSMZ Medium 1) | yes | https://mediadive.dsmz.de/medium/1 | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water |
5441 | YEAST-PEPTONE-SUCCINATE MEDIUM (DSMZ Medium 988) | yes | https://mediadive.dsmz.de/medium/988 | Name: YEAST-PEPTONE-SUCCINATE MEDIUM (DSMZ Medium 988) Composition: Agar 15.0 g/l Peptone 3.0 g/l Yeast extract 3.0 g/l Sodium succinate 2.3 g/l Distilled water |
37431 | MEDIUM 72- for trypto casein soja agar | yes | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |
116071 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 | |
116071 | CIP Medium 566 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=566 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
5441 | positive | growth | 22 | psychrophilic |
37431 | positive | growth | 22 | psychrophilic |
58157 | positive | growth | 22 | psychrophilic |
116071 | positive | growth | 10-25 | psychrophilic |
116071 | no | growth | 5 | psychrophilic |
116071 | no | growth | 30 | mesophilic |
116071 | no | growth | 37 | mesophilic |
116071 | no | growth | 41 | thermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 58157
- oxygen tolerance: aerobe
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 97 |
69480 | no | 99.985 |
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
116071 | NaCl | positive | growth | 0 % |
116071 | NaCl | no | growth | 2 % |
116071 | NaCl | no | growth | 4 % |
116071 | NaCl | no | growth | 6 % |
116071 | NaCl | no | growth | 8 % |
116071 | NaCl | no | growth | 10 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68369 | 25115 | malate | + | assimilation |
68369 | 17128 | adipate | - | assimilation |
68369 | 17306 | maltose | + | assimilation |
68369 | 59640 | N-acetylglucosamine | + | assimilation |
68369 | 16899 | D-mannitol | - | assimilation |
68369 | 16024 | D-mannose | + | assimilation |
68369 | 30849 | L-arabinose | + | assimilation |
68369 | 17634 | D-glucose | + | assimilation |
68369 | 5291 | gelatin | - | hydrolysis |
68369 | 4853 | esculin | + | hydrolysis |
68369 | 16199 | urea | - | hydrolysis |
68369 | 29016 | arginine | - | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | - | reduction |
116071 | 4853 | esculin | + | hydrolysis |
116071 | 17632 | nitrate | - | reduction |
116071 | 16301 | nitrite | - | reduction |
antibiotic resistance
- @ref: 116071
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: no
- is resistant: yes
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68369 | 35581 | indole | no |
116071 | 35581 | indole | no |
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
68369 | cytochrome oxidase | - | 1.9.3.1 |
68369 | gelatinase | - | |
68369 | beta-glucosidase | + | 3.2.1.21 |
68369 | urease | - | 3.5.1.5 |
68369 | arginine dihydrolase | - | 3.5.3.6 |
116071 | oxidase | - | |
116071 | beta-galactosidase | + | 3.2.1.23 |
116071 | alcohol dehydrogenase | - | 1.1.1.1 |
116071 | gelatinase | - | |
116071 | amylase | - | |
116071 | DNase | - | |
116071 | caseinase | - | 3.4.21.50 |
116071 | catalase | + | 1.11.1.6 |
116071 | tween esterase | - | |
116071 | lecithinase | - | |
116071 | lipase | - | |
116071 | lysine decarboxylase | - | 4.1.1.18 |
116071 | ornithine decarboxylase | - | 4.1.1.17 |
116071 | protease | - | |
116071 | tryptophan deaminase | - | |
116071 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | + | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | + | 3.2.1.31 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucosidase | + | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
fatty acid profile
fatty acids
@ref fatty acid percentage ECL 58157 C14:0 1.6 14 58157 C15:0 0.2 15 58157 C16:0 9.8 16 58157 11 methyl 18:1 ω7c 1.6 18.081 58157 C12:0 2OH 0.5 13.178 58157 C14:0 2OH 9.4 15.205 58157 C16:0 iso 3OH 0.4 17.145 58157 C16:1 2OH 0.9 17.047 58157 C16:1 ω5c 2.7 15.908 58157 C16:1 ω7c 31.7 15.819 58157 C17:1 ω6c 1.2 16.862 58157 C17:1 ω7c 0.6 16.818 58157 C18:1 ω5c 0.9 17.919 58157 C18:1 ω7c /12t/9t 38.3 17.824 58157 unknown 18.555 0.2 18.555 - type of FA analysis: whole cell analysis
- method/protocol: CCUG
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
116071 | - | + | + | + | - | + | + | - | - | - | + | + | - | - | + | + | + | - | - | - |
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
5441 | - | - | - | - | - | + | - | + | + | + | + | - | + | + | +/- | +/- | - | + | + | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | continent | country | origin.country |
---|---|---|---|---|---|
5441 | indoor air of the Sainsburry Center for Visal Art | Norwich | Europe | ||
58157 | Air | Norwich,Sainsbury Centre for Vis.Arts | Europe | United Kingdom | GBR |
116071 | Environment, Indoor air of Sainsburry Center for Visal Art | Norwich | Europe | United Kingdom | GBR |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Built environment | |
#Environmental | #Air | #Indoor Air |
taxonmaps
- @ref: 69479
- File name: preview.99_1517.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_20;96_868;97_1016;98_1199;99_1517&stattab=map
- Last taxonomy: Sphingomonas
- 16S sequence: AJ429240
- Sequence Identity:
- Total samples: 35341
- soil counts: 6757
- aquatic counts: 7018
- animal counts: 12267
- plant counts: 9299
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
5441 | 1 | Risk group (German classification) |
116071 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 20218
- description: Sphingomonas aerolata partial 16S rRNA gene, strain NW12
- accession: AJ429240
- length: 1472
- database: ena
- NCBI tax ID: 185951
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Sphingomonas aerolata NW12 | GCA_003046295 | scaffold | ncbi | 185951 |
66792 | Sphingomonas aerolata NW12 | 2740892601 | draft | img | 185951 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 97 | no |
flagellated | no | 89.805 | no |
gram-positive | no | 97.129 | no |
anaerobic | no | 99.192 | yes |
halophile | no | 92.481 | no |
spore-forming | no | 94.751 | no |
thermophile | no | 99.074 | no |
glucose-util | yes | 89.698 | no |
aerobic | yes | 92.413 | yes |
motile | yes | 67.014 | no |
glucose-ferment | no | 87.828 | no |
External links
@ref: 5441
culture collection no.: CCUG 48435, DSM 14746, LMG 21376, CIP 108369
straininfo link
- @ref: 83392
- straininfo: 86183
literature
- topic: Phylogeny
- Pubmed-ID: 13130003
- title: Sphingomonas aurantiaca sp. nov., Sphingomonas aerolata sp. nov. and Sphingomonas faeni sp. nov., air- and dustborne and Antarctic, orange-pigmented, psychrotolerant bacteria, and emended description of the genus Sphingomonas.
- authors: Busse HJ, Denner EBM, Buczolits S, Salkinoja-Salonen M, Bennasar A, Kampfer P
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.02461-0
- year: 2003
- mesh: Air Microbiology, Antarctic Regions, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Dust, Fatty Acids/analysis, Molecular Sequence Data, Phylogeny, Pigmentation, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity, Sphingomonas/*classification/genetics/isolation & purification/metabolism
- topic2: Genetics
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
5441 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14746) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-14746 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
37431 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/5983 | ||||
58157 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 48435) | https://www.ccug.se/strain?id=48435 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
68369 | Automatically annotated from API 20NE | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
83392 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID86183.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
116071 | Curators of the CIP | Collection of Institut Pasteur (CIP 108369) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108369 |