Strain identifier

BacDive ID: 14233

Type strain: Yes

Species: Sphingomonas aerolata

Strain Designation: NW12

Strain history: CIP <- 2004, P. Kämpfer, Giessen Univ., Giessen, Germany <- O. Kim: strain NW12

NCBI tax ID(s): 185951 (species)

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General

@ref: 5441

BacDive-ID: 14233

DSM-Number: 14746

keywords: genome sequence, 16S sequence, Bacteria, aerobe, psychrophilic, Gram-negative

description: Sphingomonas aerolata NW12 is an aerobe, psychrophilic, Gram-negative bacterium that was isolated from indoor air of the Sainsburry Center for Visal Art.

NCBI tax id

  • NCBI tax id: 185951
  • Matching level: species

strain history

@refhistory
5441<- H.-J. Busse; NW12 <- O. Kim
116071CIP <- 2004, P. Kämpfer, Giessen Univ., Giessen, Germany <- O. Kim: strain NW12

doi: 10.13145/bacdive14233.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Sphingomonadales
  • family: Sphingomonadaceae
  • genus: Sphingomonas
  • species: Sphingomonas aerolata
  • full scientific name: Sphingomonas aerolata Busse et al. 2003

@ref: 5441

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Sphingomonadales

family: Sphingomonadaceae

genus: Sphingomonas

species: Sphingomonas aerolata

full scientific name: Sphingomonas aerolata Busse et al. 2003

strain designation: NW12

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.835
116071negativerod-shapedyes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5441R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
5441NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
5441YEAST-PEPTONE-SUCCINATE MEDIUM (DSMZ Medium 988)yeshttps://mediadive.dsmz.de/medium/988Name: YEAST-PEPTONE-SUCCINATE MEDIUM (DSMZ Medium 988) Composition: Agar 15.0 g/l Peptone 3.0 g/l Yeast extract 3.0 g/l Sodium succinate 2.3 g/l Distilled water
37431MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
116071CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
116071CIP Medium 566yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=566

culture temp

@refgrowthtypetemperaturerange
5441positivegrowth22psychrophilic
37431positivegrowth22psychrophilic
58157positivegrowth22psychrophilic
116071positivegrowth10-25psychrophilic
116071nogrowth5psychrophilic
116071nogrowth30mesophilic
116071nogrowth37mesophilic
116071nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 58157
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
69481no97
69480no99.985

halophily

@refsaltgrowthtested relationconcentration
116071NaClpositivegrowth0 %
116071NaClnogrowth2 %
116071NaClnogrowth4 %
116071NaClnogrowth6 %
116071NaClnogrowth8 %
116071NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin+hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
1160714853esculin+hydrolysis
11607117632nitrate-reduction
11607116301nitrite-reduction

antibiotic resistance

  • @ref: 116071
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
11607135581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase-1.9.3.1
68369gelatinase-
68369beta-glucosidase+3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
116071oxidase-
116071beta-galactosidase+3.2.1.23
116071alcohol dehydrogenase-1.1.1.1
116071gelatinase-
116071amylase-
116071DNase-
116071caseinase-3.4.21.50
116071catalase+1.11.1.6
116071tween esterase-
116071lecithinase-
116071lipase-
116071lysine decarboxylase-4.1.1.18
116071ornithine decarboxylase-4.1.1.17
116071protease-
116071tryptophan deaminase-
116071urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    58157C14:01.614
    58157C15:00.215
    58157C16:09.816
    5815711 methyl 18:1 ω7c1.618.081
    58157C12:0 2OH0.513.178
    58157C14:0 2OH9.415.205
    58157C16:0 iso 3OH0.417.145
    58157C16:1 2OH0.917.047
    58157C16:1 ω5c2.715.908
    58157C16:1 ω7c31.715.819
    58157C17:1 ω6c1.216.862
    58157C17:1 ω7c0.616.818
    58157C18:1 ω5c0.917.919
    58157C18:1 ω7c /12t/9t38.317.824
    58157unknown 18.5550.218.555
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116071-+++-++---++--+++---

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
5441-----+-++++-+++/-+/--++--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcontinentcountryorigin.country
5441indoor air of the Sainsburry Center for Visal ArtNorwichEurope
58157AirNorwich,Sainsbury Centre for Vis.ArtsEuropeUnited KingdomGBR
116071Environment, Indoor air of Sainsburry Center for Visal ArtNorwichEuropeUnited KingdomGBR

isolation source categories

Cat1Cat2Cat3
#Engineered#Built environment
#Environmental#Air#Indoor Air

taxonmaps

  • @ref: 69479
  • File name: preview.99_1517.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_20;96_868;97_1016;98_1199;99_1517&stattab=map
  • Last taxonomy: Sphingomonas
  • 16S sequence: AJ429240
  • Sequence Identity:
  • Total samples: 35341
  • soil counts: 6757
  • aquatic counts: 7018
  • animal counts: 12267
  • plant counts: 9299

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
54411Risk group (German classification)
1160711Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Sphingomonas aerolata partial 16S rRNA gene, strain NW12
  • accession: AJ429240
  • length: 1472
  • database: ena
  • NCBI tax ID: 185951

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Sphingomonas aerolata NW12GCA_003046295scaffoldncbi185951
66792Sphingomonas aerolata NW122740892601draftimg185951

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno97no
flagellatedno89.805no
gram-positiveno97.129no
anaerobicno99.192yes
halophileno92.481no
spore-formingno94.751no
thermophileno99.074no
glucose-utilyes89.698no
aerobicyes92.413yes
motileyes67.014no
glucose-fermentno87.828no

External links

@ref: 5441

culture collection no.: CCUG 48435, DSM 14746, LMG 21376, CIP 108369

straininfo link

  • @ref: 83392
  • straininfo: 86183

literature

  • topic: Phylogeny
  • Pubmed-ID: 13130003
  • title: Sphingomonas aurantiaca sp. nov., Sphingomonas aerolata sp. nov. and Sphingomonas faeni sp. nov., air- and dustborne and Antarctic, orange-pigmented, psychrotolerant bacteria, and emended description of the genus Sphingomonas.
  • authors: Busse HJ, Denner EBM, Buczolits S, Salkinoja-Salonen M, Bennasar A, Kampfer P
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.02461-0
  • year: 2003
  • mesh: Air Microbiology, Antarctic Regions, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Dust, Fatty Acids/analysis, Molecular Sequence Data, Phylogeny, Pigmentation, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity, Sphingomonas/*classification/genetics/isolation & purification/metabolism
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5441Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14746)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14746
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37431Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5983
58157Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 48435)https://www.ccug.se/strain?id=48435
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
83392Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID86183.1StrainInfo: A central database for resolving microbial strain identifiers
116071Curators of the CIPCollection of Institut Pasteur (CIP 108369)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108369