Strain identifier

BacDive ID: 14205

Type strain: No

Species: Sphingomonas sp.

Strain Designation: HH69

Strain history: <- B. Happe, Ges.Biotechn. Forschg.; HH69-3 <- H. Poth <- H. Harms; HH69 (Pseudomonas sp.)

NCBI tax ID(s): 28214 (species)

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General

@ref: 2899

BacDive-ID: 14205

DSM-Number: 7135

keywords: Bacteria, mesophilic

description: Sphingomonas sp. HH69 is a mesophilic bacterium that was isolated from farmland and forest soil.

NCBI tax id

  • NCBI tax id: 28214
  • Matching level: species

strain history

  • @ref: 2899
  • history: <- B. Happe, Ges.Biotechn. Forschg.; HH69-3 <- H. Poth <- H. Harms; HH69 (Pseudomonas sp.)

doi: 10.13145/bacdive14205.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Sphingomonadales
  • family: Sphingomonadaceae
  • genus: Sphingomonas
  • species: Sphingomonas sp.
  • full scientific name: Sphingomonas Yabuuchi et al. 1990

@ref: 2899

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Sphingomonadales

family: Sphingomonadaceae

genus: Sphingomonas

species: Sphingomonas sp.

full scientific name: Sphingomonas sp.

strain designation: HH69

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2899CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
2899MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457)yeshttps://mediadive.dsmz.de/medium/457Name: MINERAL MEDIUM (BRUNNER) (DSMZ Medium 457) Composition: Na2HPO4 2.44 g/l KH2PO4 1.52 g/l (NH4)2SO4 0.5 g/l MgSO4 x 7 H2O 0.2 g/l CaCl2 x 2 H2O 0.05 g/l EDTA 0.005 g/l FeSO4 x 7 H2O 0.002 g/l H3BO3 0.0003 g/l CoCl2 x 6 H2O 0.0002 g/l ZnSO4 x 7 H2O 0.0001 g/l MnCl2 x 4 H2O 3e-05 g/l Na2MoO4 x 2 H2O 3e-05 g/l NiCl2 x 6 H2O 2e-05 g/l CuCl2 x 2 H2O 1e-05 g/l Distilled water

culture temp

  • @ref: 2899
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate-assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin+hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369gelatinase-
68369beta-glucosidase+3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    50468C16:08.716
    50468C14:0 2OH7.815.205
    50468C16:0 2OH1.417.233
    50468C16:1 ω5c2.915.908
    50468C16:1 ω7c15.215.819
    50468C18:1 ω5c4.417.919
    50468C18:1 ω7c /12t/9t59.717.824
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
50468-+---+----++---+----

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
50468+----+-++----+----+--
2899-----+--++----------+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
2899farmland and forest soilnear HamburgGermanyDEUEurope
50468Soil,noncontaminated,enrichment with dibenzofurannear LimburgGermanyDEUEurope1987-10-01

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture
#Environmental#Terrestrial#Forest
#Environmental#Terrestrial#Soil

Sequence information

GC content

  • @ref: 2899
  • GC-content: 61.0

External links

@ref: 2899

culture collection no.: DSM 7135, CCUG 31190

straininfo link

  • @ref: 83364
  • straininfo: 48369

Reference

@idauthorscataloguedoi/urltitle
2899Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7135)https://www.dsmz.de/collection/catalogue/details/culture/DSM-7135
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
50468Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 31190)https://www.ccug.se/strain?id=31190
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
83364Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID48369.1StrainInfo: A central database for resolving microbial strain identifiers