Strain identifier

BacDive ID: 1419

Type strain: Yes

Species: Geobacillus stearothermophilus

Strain Designation: R-35646, 26

Strain history: CIP <- 1966, ATCC <- NCA: strain 26

NCBI tax ID(s): 1366047 (strain), 1422 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 2023

BacDive-ID: 1419

DSM-Number: 22

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, chemoorganotroph, spore-forming, thermophilic, motile, rod-shaped, colony-forming

description: Geobacillus stearothermophilus R-35646 is a facultative anaerobe, chemoorganotroph, spore-forming bacterium that forms circular colonies and was isolated from deteriorated canned food.

NCBI tax id

NCBI tax idMatching level
1366047strain
1422species

strain history

@refhistory
2023<- ATCC <- NCA, 26
67770ATCC 12980 <-- Natl. Canner's Assoc., Washington; NCA 26.
67772ATCC <- NCA, 26
123299CIP <- 1966, ATCC <- NCA: strain 26

doi: 10.13145/bacdive1419.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Geobacillus
  • species: Geobacillus stearothermophilus
  • full scientific name: Geobacillus stearothermophilus (Donk 1920) Nazina et al. 2001
  • synonyms

    • @ref: 20215
    • synonym: Bacillus stearothermophilus

@ref: 2023

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Geobacillus

species: Geobacillus stearothermophilus

full scientific name: Geobacillus stearothermophilus (Donk 1920) Nazina et al. 2001 emend. Coorevits et al. 2012

strain designation: R-35646, 26

type strain: yes

Morphology

cell morphology

@refcell lengthcell widthcell shapemotilityflagellum arrangementgram stain
230422.0-3.5 µm0.6-1.0 µmrod-shapedyesperitrichous
123299rod-shapedyespositive

colony morphology

  • @ref: 23042
  • colony size: 0.5-2.0 mm
  • colony shape: circular
  • incubation period: 1-2 days
  • medium used: trypticase soy agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
23042trypticase soy agaryes
2023CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://mediadive.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
36276MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
123299CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
123299CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
22918positivemaximum60thermophilic
22918positiveminimum40thermophilic
23042positivegrowth40.0-60.0thermophilic
23042maximum60.0-70.0thermophilic
23042minimum30.0-45.0
23042thermophilic
2023positivegrowth55thermophilic
36276positivegrowth55thermophilic
48397positivegrowth55thermophilic
67770positivegrowth55thermophilic
67772positiveoptimum55-65thermophilic
67772positiveminimum37mesophilic
67772positivemaximum75thermophilic
123299positivegrowth45-55thermophilic
123299nogrowth10psychrophilic
123299nogrowth22psychrophilic
123299nogrowth30mesophilic
123299nogrowth37mesophilic

culture pH

@refabilitytypepHPH range
22918positivegrowth9alkaliphile
22918nogrowth5
23042positivegrowth6.0
23042positivegrowth9.0
23042nogrowth5.0
67772positivemaximum7.5
67772positiveminimum6.2
123299nogrowth6

Physiology and metabolism

oxygen tolerance

  • @ref: 123299
  • oxygen tolerance: facultative anaerobe

nutrition type

  • @ref: 23042
  • type: chemoorganotroph

spore formation

@refspore formationspore descriptiontype of spore
22918yes
23042yesellipsoidal,subterminal and/or terminal,in sporangiaspore
123299yes

compound production

@refcompound
2023restriction endonuclease BstPI
2023aspartate transaminase
2023phosphinothricin
22918Acetoin

halophily

@refsaltgrowthtested relationconcentration
22918NaClpositivegrowth1 %(w/v)
22918NaClnogrowth5 %(w/v)
23042NaClpositivegrowth1.0 %(w/v)
23042NaClnogrowth5.0 %(w/v)
123299NaClpositivegrowth0-2 %
123299NaClnogrowth4 %
123299NaClnogrowth6 %
123299NaClnogrowth8 %
123299NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: Biological indicator for moist heat sterilization (Terminal sterilization and sterilization indicators, Japanese Pharmacopoeia, 16 ed.)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2291827613amygdalin-builds acid from
2291818305arbutin-builds acid from
2291817057cellobiose-builds acid from
2291865327D-xylose-builds acid from
2291828260galactose+/-builds acid from
229185291gelatin+hydrolysis
2291828066gentiobiose-builds acid from
2291817754glycerol+/-builds acid from
2291828087glycogen+/-builds acid from
2291830849L-arabinose-builds acid from
2291817716lactose-builds acid from
2291829864mannitol-builds acid from
229186731melezitose+builds acid from
2291828053melibiose+builds acid from
2291837657methyl D-glucoside+builds acid from
2291817268myo-inositol-builds acid from
22918506227N-acetyl-D-glucosamine-builds acid from
22918O-nitrophenyl-beta-D-galactopyranosid-hydrolysis
2291816634raffinose+builds acid from
2291833942ribose-builds acid from
2291817814salicin-builds acid from
2291828017starch+hydrolysis
2291817992sucrose+builds acid from
2291827082trehalose+builds acid from
2291832528turanose+builds acid from
23042168082-dehydro-D-gluconate-builds acid from
23042174265-dehydro-D-gluconate-builds acid from
2304215963ribitol-builds acid from
2304227613amygdalin-builds acid from
2304218305arbutin-builds acid from
2304217057cellobiose-builds acid from
2304217108D-arabinose-builds acid from
2304218333D-arabitol-builds acid from
2304228847D-fucose-builds acid from
2304262318D-lyxose-builds acid from
2304216443D-tagatose-builds acid from
2304265327D-xylose-builds acid from
2304216813galactitol-builds acid from
2304217113erythritol-builds acid from
2304228066gentiobiose-builds acid from
2304224265gluconate-builds acid from
2304230849L-arabinose-builds acid from
2304218403L-arabitol-builds acid from
2304218287L-fucose-builds acid from
2304217266L-sorbose-builds acid from
2304265328L-xylose-builds acid from
2304217716lactose-builds acid from
2304229864mannitol-builds acid from
2304217268myo-inositol-builds acid from
23042506227N-acetylglucosamine-builds acid from
2304226546rhamnose-builds acid from
2304233942ribose-builds acid from
2304217814salicin-builds acid from
2304230911sorbitol-builds acid from
2304217151xylitol-builds acid from
2304215824D-fructose-builds gas from
2304217634D-glucose-builds gas from
2304216024D-mannose-builds gas from
2304228260galactose-builds gas from
2304217754glycerol-builds gas from
2304228087glycogen-builds gas from
2304217306maltose-builds gas from
230426731melezitose-builds gas from
2304228053melibiose-builds gas from
2304237657methyl D-glucoside-builds gas from
2304216634raffinose-builds gas from
2304228017starch-builds gas from
2304217992sucrose-builds gas from
2304227082trehalose-builds gas from
2304232528turanose-builds gas from
2304216947citrate-carbon source
2304227897tryptophan-energy source
23042O-nitrophenyl-beta-D-galactopyranosid-hydrolysis
2304215824D-fructose+builds acid from
2304217634D-glucose+builds acid from
2304216024D-mannose+builds acid from
2304228260galactose+builds acid from
2304217754glycerol+builds acid from
2304228087glycogen+builds acid from
2304215443inulin+builds acid from
2304217306maltose+builds acid from
230426731melezitose+builds acid from
2304228053melibiose+builds acid from
2304237657methyl D-glucoside+builds acid from
2304216634raffinose+builds acid from
2304228017starch+builds acid from
2304217992sucrose+builds acid from
2304227082trehalose+builds acid from
2304232528turanose+builds acid from
23042casein+hydrolysis
230424853esculin+hydrolysis
230425291gelatin+hydrolysis
2304228017starch+hydrolysis
2304217632nitrate+reduction
12329916947citrate-carbon source
1232994853esculin-hydrolysis
123299606565hippurate-hydrolysis
12329917632nitrate+reduction
12329916301nitrite+reduction
12329917632nitrate+respiration

metabolite production

@refChebi-IDmetaboliteproduction
2291815688acetoinyes
2304235581indoleno
2304216136hydrogen sulfideno
2304215688acetoinyes
12329935581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testcitrate testmethylred-test
2291815688acetoin+
2304215688acetoin+
2304235581indole-
2304216947citrate-
12329915688acetoin-
12329917234glucose+

enzymes

@refvalueactivityec
22918beta-galactosidase-3.2.1.23
22918catalase+1.11.1.6
22918gelatinase+
22918cytochrome oxidase-1.9.3.1
23042arginine dihydrolase-3.5.3.6
23042beta-galactosidase-3.2.1.23
23042catalase+1.11.1.6
23042cytochrome oxidase-1.9.3.1
23042lysine decarboxylase-4.1.1.18
23042ornithine decarboxylase-4.1.1.17
23042tryptophan deaminase-4.1.99.1
23042urease-3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
123299oxidase+
123299beta-galactosidase-3.2.1.23
123299alcohol dehydrogenase+1.1.1.1
123299gelatinase+/-
123299amylase+
123299DNase+
123299caseinase+3.4.21.50
123299catalase-1.11.1.6
123299tween esterase+
123299gamma-glutamyltransferase-2.3.2.2
123299lecithinase-
123299lipase-
123299lysine decarboxylase-4.1.1.18
123299ornithine decarboxylase-4.1.1.17
123299protease-
123299urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    48397C16:010.716
    48397C15:0 ANTEISO10.914.711
    48397C15:0 ISO36.814.621
    48397C16:0 iso515.626
    48397C17:0 anteiso23.416.722
    48397C17:0 iso13.216.629
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123299--++-+---++-+--+----

Isolation, sampling and environmental information

isolation

@refsample typesampling date
2023deteriorated canned food
67772Deteriorated canned food1990
123299Food, Canned food

isolation source categories

Cat1Cat2Cat3
#Engineered#Biodegradation#Composting
#Engineered#Food production#Bottled, canned, packed food

taxonmaps

  • @ref: 69479
  • File name: preview.99_2201.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_79;96_191;97_207;98_231;99_2201&stattab=map
  • Last taxonomy: Geobacillus
  • 16S sequence: LC177207
  • Sequence Identity:
  • Total samples: 3216
  • soil counts: 387
  • aquatic counts: 603
  • animal counts: 2094
  • plant counts: 132

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
20231Risk group (German classification)
677721
1232991Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Geobacillus stearothermophilus gene for 16S rRNA, partial sequenceAB2717571485ena1422
20218Geobacillus stearothermophilus clone EM-2_F 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF478064628ena1366047
20218Bacillus stearothermophilus 16S rRNA gene, strain DSM 22TAJ2948171505ena1422
20218Geobacillus stearothermophilus strain BGSC 9A20 16S ribosomal RNA gene, complete sequenceAY6089281562ena1422
20218Geobacillus stearothermophilus strain DSM 22 16S-23S ribosomal RNA intergenic spacer and tRNA-Ile gene, partial sequence; and tRNA-Ala gene, complete sequenceEU157949284ena1422
20218Geobacillus stearothermophilus isolate Gst2 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceEU723631628ena1422
20218Geobacillus stearothermophilus isolate Gst6 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceEU723632628ena1422
20218Geobacillus stearothermophilus isolate Gst39 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceEU723633502ena1422
2023Geobacillus stearothermophilus partial 16S rRNA gene, strain R-35646FN4286941511ena1422
67770Geobacillus stearothermophilus gene for 16S ribosomal RNA, partial sequence, strain: JCM 2501LC1772071481ena1422
67770B.stearothermophilus 16S ribosomal RNAX606401434ena1422

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Geobacillus stearothermophilus ATCC 129801366047.4wgspatric1366047
66792Geobacillus stearothermophilus ATCC 129802654587565draftimg1366047
67770Geobacillus stearothermophilus ATCC 12980GCA_001277805scaffoldncbi1366047

GC content

@refGC-contentmethod
202352.8high performance liquid chromatography (HPLC)
202351.9thermal denaturation, midpoint method (Tm)
202351.5Buoyant density centrifugation (BD)
6777051.6thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes89.919no
gram-positiveyes89.654no
anaerobicno97.513no
halophileno92.302yes
spore-formingyes94.299yes
glucose-utilyes90.275no
thermophileyes100no
flagellatedyes87.275yes
aerobicyes84.7no
glucose-fermentno86.279no

External links

@ref: 2023

culture collection no.: DSM 22, ATCC 12980, CCM 2062, CCUG 26241, IAM 11062, LMG 6939, NBRC 12550, NCIMB 8923, NCTC 10339, VKM B-2231, IFO 12550, NCA 26, JCM 2501, BCRC 11092, CIP 66.23, KCTC 1665, KCTC 1752, NCFB 1768, NRRL B-1172, VKM B-510, VTT E-81128, UCCCB118, NCDO 1768

straininfo link

  • @ref: 71070
  • straininfo: 1198

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology1368771Construction of an Escherichia coli export-affinity vector for expression and purification of foreign proteins by fusion to cyclomaltodextrin glucanotransferase.Hellman J, Mantsala PJ Biotechnol10.1016/0168-1656(92)90097-s1992Amino Acid Sequence, Base Sequence, Catalysis, Enzyme Stability, Escherichia coli/chemistry/enzymology/*genetics, *Genetic Vectors, Glucosyltransferases/*genetics, Hydrolysis, Molecular Sequence Data, Mutagenesis, Recombinant Fusion Proteins/biosynthesis/chemistry/*isolation & purificationGenetics
Enzymology3389970Localized insertion of new S-layer during growth of Bacillus stearothermophilus strains.Gruber K, Sleytr UBArch Microbiol10.1007/BF004467491988Bacterial Proteins/*isolation & purification, Cell Wall/ultrastructure, Geobacillus stearothermophilus/cytology/*growth & development/ultrastructure, Immune Sera, Macromolecular Substances, Membrane Proteins/*isolation & purification, Microscopy, ElectronPhylogeny
Cultivation3566270Thermal inactivation and injury of Bacillus stearothermophilus spores.Feeherry FE, Munsey DT, Rowley DBAppl Environ Microbiol10.1128/aem.53.2.365-370.19871987Anaerobiosis, Culture Media, Food Microbiology, Geobacillus stearothermophilus/*physiology, Hot Temperature, Hydrogen-Ion Concentration, Kinetics, Spores, BacterialBiotechnology
Pathogenicity5132091Rapid quantitative microbiological assay of antibiotics and chemical preservatives of a nonantibiotic nature.Kabay AAppl Microbiol10.1128/am.22.5.752-755.19711971Agar, Anti-Bacterial Agents/*pharmacology/urine, Bacillus/drug effects, Bacteria/*drug effects, *Biological Assay/standards, Diffusion, Drug Resistance, Microbial, Humans, Methods, Pharmaceutic Aids/analysis, Time Factors
Enzymology6277855Transformation of Bacillus stearothermophilus with plasmid DNA and characterization of shuttle vector plasmids between Bacillus stearothermophilus and Bacillus subtilis.Imanaka T, Fujii M, Aramori I, Aiba SJ Bacteriol10.1128/jb.149.3.824-830.19821982Bacillus subtilis/*genetics, DNA Restriction Enzymes, DNA, Bacterial, *Genetic Vectors, Geobacillus stearothermophilus/*genetics, *Plasmids, Protoplasts, Temperature, *Transformation, Bacterial
Pathogenicity6430864Rapid assay for tetracycline in premixes and mixed feeds.Katz JM, Katz SEJ Assoc Off Anal Chem1984Animal Feed/*analysis, Bacillus cereus/drug effects, Chlortetracycline/analysis, Geobacillus stearothermophilus/drug effects, Microbial Sensitivity Tests, Oxytetracycline/analysis, Tetracyclines/*analysis
Pathogenicity7757456Effect of rubber stopper composition, preservative pretreatment and rinse water temperature on the moist heat resistance of Bacillus stearothermophilus ATCC 12980.Rubio SL, Moldenhauer JEPDA J Pharm Sci Technol1995Drug Packaging, Geobacillus stearothermophilus/drug effects/*physiology, Preservatives, Pharmaceutical/*pharmacology, Rubber, Spores, Bacterial, *Sterilization, Temperature
Metabolism8824603Evidence that an N-terminal S-layer protein fragment triggers the release of a cell-associated high-molecular-weight amylase in Bacillus stearothermophilus ATCC 12980.Egelseer EM, Schocher I, Sleytr UB, Sara MJ Bacteriol10.1128/jb.178.19.5602-5609.19961996Amylases/*metabolism, Bacterial Outer Membrane Proteins/*metabolism, *Bacterial Proteins, Cell Membrane/ultrastructure, Cell Wall/metabolism, Genetic Variation, Geobacillus stearothermophilus/drug effects/*enzymology/ultrastructure, Guanidine, Guanidines/pharmacology, *Membrane Glycoproteins, Microscopy, Immunoelectron, Models, Biological, Molecular Weight, Peptide Fragments/metabolism, Subcellular Fractions/metabolismEnzymology
Enzymology9349714A novel levansucrase-levanase gene cluster in Bacillus stearothermophilus ATCC12980.Li Y, Triccas JA, Ferenci TBiochim Biophys Acta10.1016/s0167-4781(97)00103-61997Amino Acid Sequence, *Bacterial Proteins, Base Sequence, Cloning, Molecular, Escherichia coli/genetics/metabolism, Genes, Bacterial/*genetics, Geobacillus stearothermophilus/enzymology/*genetics, Glycoside Hydrolases/*genetics, Hexosyltransferases/*genetics, Molecular Sequence Data, Multigene Family/*genetics, Open Reading Frames/genetics, Sequence Analysis, DNA, Sequence Homology, Amino AcidGenetics
Metabolism9515918The S-layer proteins of two Bacillus stearothermophilus wild-type strains are bound via their N-terminal region to a secondary cell wall polymer of identical chemical composition.Egelseer EM, Leitner K, Jarosch M, Hotzy C, Zayni S, Sleytr UB, Sara MJ Bacteriol10.1128/JB.180.6.1488-1495.19981998Amino Acid Sequence, Bacterial Capsules/*chemistry/*metabolism/ultrastructure, Cell Wall/*chemistry/metabolism/ultrastructure, Crystallization, Geobacillus stearothermophilus/*chemistry/metabolism, Glycoconjugates/chemistry/metabolism, Hydrofluoric Acid/pharmacology, Peptide Mapping, Polymers/chemistry/metabolism, Protein Binding, Protein Conformation, Sequence Analysis, Serine Endopeptidases/pharmacologyTranscriptome
Stress9849781Predictive model to describe the combined effect of pH and NaCl on apparent heat resistance of Bacillus stearothermophilus.Periago PM, Fernandez PS, Salmeron MC, Martinez AInt J Food Microbiol10.1016/s0168-1605(98)00111-11998Geobacillus stearothermophilus/*physiology, Hot Temperature, Hydrogen-Ion Concentration, Models, Biological, Sodium Chloride/*pharmacology
Metabolism10708365S-layer gene sbsC of Bacillus stearothermophilus ATCC 12980: molecular characterization and heterologous expression in Escherichia coli.Jarosch M, Egelseer EM, Mattanovich D, Sleytr UB, Sara MMicrobiology (Reading)10.1099/00221287-146-2-2732000Bacterial Proteins/chemistry/*genetics/*metabolism, Base Sequence, Blotting, Northern, Cell Wall/metabolism, Cloning, Molecular, DNA Primers, DNA, Bacterial/analysis/genetics, Electrophoresis, Polyacrylamide Gel, Escherichia coli/*genetics/metabolism, Geobacillus stearothermophilus/*genetics/growth & development/metabolism, Immunoblotting, Membrane Glycoproteins/chemistry/*genetics/*metabolism, Molecular Sequence Data, Recombinant Proteins/chemistry/metabolism, Sequence Analysis, DNA, Structure-Activity RelationshipEnzymology
Phylogeny10843079Bacillus thermodenitrificans sp. nov., nom. rev.Manachini PL, Mora D, Nicastro G, Parini C, Stackebrandt E, Pukall R, Fortina MGInt J Syst Evol Microbiol10.1099/00207713-50-3-13312000Bacillus/chemistry/*classification/isolation & purification/physiology, Base Composition, DNA Fingerprinting, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Molecular Sequence Data, Nitrates/*metabolism, Nucleic Acid Hybridization, Phenotype, RNA, Ribosomal, 16S/genetics, RNA, Ribosomal, 23S/genetics, Restriction Mapping, *Soil Microbiology, Temperature, rRNA Operon/geneticsGenetics
Metabolism10974105ISBst12, a novel type of insertion-sequence element causing loss of S-layer-gene expression in Bacillus stearothermophilus ATCC 12980.Egelseer EM, Idris R, Jarosch M, Danhorn T, Sleytr UB, Sara MMicrobiology (Reading)10.1099/00221287-146-9-21752000Amino Acid Sequence, Bacterial Proteins/*genetics/*metabolism, Blotting, Northern, Blotting, Southern, *DNA Transposable Elements, Geobacillus stearothermophilus/*genetics/growth & development/metabolism, Membrane Glycoproteins/*genetics/*metabolism, Molecular Sequence Data, Sequence Analysis, DNA, Transposases/geneticsGenetics
Metabolism11320138Analysis of the structure-function relationship of the S-layer protein SbsC of Bacillus stearothermophilus ATCC 12980 by producing truncated forms.Jarosch M, Egelseer EM, Huber C, Moll D, Mattanovich D, Sleytr UB, Sara MMicrobiology (Reading)10.1099/00221287-147-5-13532001Bacterial Proteins/chemistry/*genetics/metabolism, Cell Wall/metabolism, Chromatography, Affinity, Electrophoresis, Polyacrylamide Gel, Escherichia coli/genetics/metabolism/ultrastructure, Geobacillus stearothermophilus/chemistry/*genetics, Membrane Glycoproteins/chemistry/*genetics/metabolism, Microscopy, Electron, Molecular Sequence Data, Peptidoglycan/chemistry, Polymerase Chain Reaction, Protein Binding, Protein Structure, Tertiary, Recombinant Proteins/genetics/metabolism, Sequence Deletion, Structure-Activity RelationshipEnzymology
Metabolism11797047Characterization of an S-layer glycoprotein produced in the course of S-layer variation of Bacillus stearothermophilus ATCC 12980 and sequencing and cloning of the sbsD gene encoding the protein moiety.Egelseer EM, Danhorn T, Pleschberger M, Hotzy C, Sleytr UB, Sara MArch Microbiol10.1007/s00203-001-0363-52001Amino Acid Sequence, Bacterial Proteins/*genetics/*metabolism, Cell Wall/chemistry/*metabolism, Genetic Variation, Geobacillus stearothermophilus/*genetics/growth & development/metabolism/ultrastructure, Membrane Glycoproteins/*genetics/metabolism, Microscopy, Electron, Molecular Sequence Data, Peptidoglycan/analysis, Polymers/analysis, Sequence Analysis, DNAGenetics
11932495A recombinant bacterial cell surface (S-layer)-major birch pollen allergen-fusion protein (rSbsC/Bet v1) maintains the ability to self-assemble into regularly structured monomolecular lattices and the functionality of the allergen.Breitwieser A, Egelseer EM, Moll D, Ilk N, Hotzy C, Bohle B, Ebner C, Sleytr UB, Sara MProtein Eng10.1093/protein/15.3.2432002Allergens/*genetics/immunology, Antigens, Plant, Bacterial Proteins/*genetics, Crystallization, Geobacillus stearothermophilus, Immunoblotting, Immunoglobulin E/immunology, Membrane Glycoproteins/*genetics, Plant Proteins/*genetics/immunology, Protein Engineering, Recombinant Fusion Proteins/*chemistry/genetics/*immunology
Enzymology12297034The purification and characterization of a Bacillus stearothermophilus methionine aminopeptidase (MetAP).Chung JM, Chung IY, Lee YSJ Biochem Mol Biol10.5483/bmbrep.2002.35.2.2282002Aminopeptidases/antagonists & inhibitors/chemistry/*isolation & purification/metabolism, Cations, Divalent/pharmacology, Chelating Agents/pharmacology, Chemical Phenomena, Chemistry, Physical, Geobacillus stearothermophilus/*enzymology, Hydrogen-Ion Concentration, Kinetics, Methionyl Aminopeptidases, Molecular Weight, Oligopeptides/chemistry, Protease Inhibitors/pharmacology, Protein Subunits, Substrate Specificity, TemperaturePhylogeny
Biotechnology12810270A RAPD-based comparison of thermophilic bacilli from milk powders.Ronimus RS, Parker LE, Turner N, Poudel S, Ruckert A, Morgan HWInt J Food Microbiol10.1016/s0168-1605(02)00480-42003Animals, Bacillus/*classification/genetics, Base Sequence, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, *Food Microbiology, Milk/*microbiology, Molecular Sequence Data, *Random Amplified Polymorphic DNA Technique, Sequence Homology, Nucleic AcidGenetics
Phylogeny12901420Genetic polymorphism by RAPD-PCR and phenotypic characteristics of isolated thermotolerant Bacillus strains from hot spring sources.Hazem A, Manar ANew Microbiol2003Bacillus/*classification/*genetics/metabolism, Cluster Analysis, DNA Fingerprinting, DNA, Bacterial/chemistry/genetics, Hot Temperature, Hydrogen-Ion Concentration, Jordan, Microbial Sensitivity Tests, Phenotype, Phylogeny, Polymorphism, Genetic, Random Amplified Polymorphic DNA Technique, *Water MicrobiologyStress
Stress14558699A method of increasing test range and accuracy of bioindicators: Geobacillus stearothermophilus spores.Lundahl GPDA J Pharm Sci Technol2003Drug Stability, Drug Storage, *Geobacillus stearothermophilus, *Glycerol, Hot Temperature, *Spores, Bacterial, Sterilization/*methods, Suspensions, *Technology, Pharmaceutical, *Temperature
Enzymology15033540Molecular cloning and characterization of two thermostable carboxyl esterases from Geobacillus stearothermophilus.Ewis HE, Abdelal AT, Lu CDGene10.1016/j.gene.2003.12.0292004Carboxylic Ester Hydrolases/*genetics/isolation & purification/metabolism, DNA, Bacterial/chemistry/genetics/isolation & purification, Dithiothreitol/pharmacology, Enzyme Activation/drug effects, Enzyme Stability, Geobacillus stearothermophilus/enzymology/*genetics, Hydrogen-Ion Concentration, Isoenzymes/antagonists & inhibitors/genetics/metabolism, Kinetics, Mercaptoethanol/pharmacology, Molecular Sequence Data, Sequence Analysis, DNA, Substrate Specificity, Temperature, p-Chloromercuribenzoic Acid/pharmacologyPhylogeny
Metabolism16086256Screening of catalytically active microorganisms for the synthesis of 6-modified purine nucleosides.Trelles JA, Valino AL, Runza V, Lewkowicz ES, Iribarren AMBiotechnol Lett10.1007/s10529-005-5628-72005Bacteria/enzymology/isolation & purification/*metabolism, Catalysis, Geobacillus stearothermophilus/enzymology/*metabolism, Glycosylation, Industrial Microbiology/economics/methods, Nucleosides/metabolism, Purine Nucleosides/*biosynthesis, Purine-Nucleoside Phosphorylase/metabolismEnzymology
Metabolism17644609High-affinity interaction between the S-layer protein SbsC and the secondary cell wall polymer of Geobacillus stearothermophilus ATCC 12980 determined by surface plasmon resonance technology.Ferner-Ortner J, Mader C, Ilk N, Sleytr UB, Egelseer EMJ Bacteriol10.1128/JB.00294-072007Bacterial Proteins/chemistry/genetics/*metabolism, Cell Wall/*metabolism, Electrophoresis, Polyacrylamide Gel, Geobacillus stearothermophilus/*metabolism, Kinetics, Peptide Fragments/chemistry/metabolism, Protein Binding, Surface Plasmon Resonance/*methodsEnzymology
Pathogenicity18025169A novel approach to specific allergy treatment: the recombinant allergen-S-layer fusion protein rSbsC-Bet v 1 matures dendritic cells that prime Th0/Th1 and IL-10-producing regulatory T cells.Gerstmayr M, Ilk N, Schabussova I, Jahn-Schmid B, Egelseer EM, Sleytr UB, Ebner C, Bohle BJ Immunol10.4049/jimmunol.179.11.72702007Allergens/genetics/*immunology, Antigens, Plant, Bacterial Proteins/genetics/immunology, Cell Differentiation/immunology, Dendritic Cells/*immunology/virology, Humans, Hypersensitivity/therapy, Interleukin-10/biosynthesis/immunology, Interleukin-12/biosynthesis/immunology, Membrane Glycoproteins/genetics/immunology, Monocytes/immunology/virology, Phenotype, Recombinant Fusion Proteins/genetics/*immunology, T-Lymphocytes, Regulatory/*immunology, Th1 Cells/*immunology, Th2 Cells/immunology, Up-Regulation/immunologyPhenotype
Enzymology18026230Menaquinone reduction by an HMT2-like sulfide dehydrogenase from Bacillus stearothermophilus.Shibata H, Suzuki K, Kobayashi SCan J Microbiol10.1139/W07-0772007Amino Acid Sequence, Anaerobiosis, Cell Membrane/enzymology, Enzyme Stability, Escherichia coli/enzymology/genetics, Fumarates/metabolism, Geobacillus stearothermophilus/*enzymology/genetics/growth & development, Hot Temperature, Kinetics, Molecular Sequence Data, Nitrates, Oxidation-Reduction, Oxidoreductases/genetics/*metabolism, Sulfides/*metabolism, Vitamin K 2/*metabolismMetabolism
Metabolism19460092The high-molecular-mass amylase (HMMA) of Geobacillus stearothermophilus ATCC 12980 interacts with the cell wall components by virtue of three specific binding regions.Ferner-Ortner-Bleckmann J, Huber-Gries C, Pavkov T, Keller W, Mader C, Ilk N, Sleytr UB, Egelseer EMMol Microbiol10.1111/j.1365-2958.2009.06734.x2009Amylases/genetics/*metabolism, Bacterial Proteins/genetics/*metabolism, Cell Wall/*metabolism, Cloning, Molecular, Genes, Bacterial, Geobacillus stearothermophilus/*enzymology/genetics, Peptidoglycan/metabolism, Protein Binding, Sequence Alignment, Sequence Analysis, ProteinEnzymology
19851018Towards the structure of the C-terminal part of the S-layer protein SbsC.Kroutil M, Pavkov T, Birner-Gruenberger R, Tesarz M, Sleytr UB, Egelseer EM, Keller WActa Crystallogr Sect F Struct Biol Cryst Commun10.1107/S17443091090353862009Bacterial Proteins/*chemistry, Crystallization, Crystallography, X-Ray, Geobacillus stearothermophilus, Membrane Glycoproteins/*chemistry, Peptide Fragments/chemistry, Protein Structure, Tertiary
Metabolism23192035Crystallization of domains involved in self-assembly of the S-layer protein SbsC.Ethordic A, Egelseer EM, Tesarz M, Sleytr UB, Keller W, Pavkov-Keller TActa Crystallogr Sect F Struct Biol Cryst Commun10.1107/S17443091120426502012Bacterial Proteins/*chemistry/metabolism, Cell Wall/metabolism, Crystallization, Crystallography, X-Ray, Geobacillus stearothermophilus/*metabolism, Membrane Glycoproteins/*chemistry/metabolism, Protein Structure, Tertiary
23504021Small-angle X-ray scattering for imaging of surface layers on intact bacteria in the native environment.Sekot G, Schuster D, Messner P, Pum D, Peterlik H, Schaffer CJ Bacteriol10.1128/JB.02164-122013Bacteria/ultrastructure, Comamonadaceae, Geobacillus stearothermophilus, Microscopy, Electron, Transmission, *Scattering, Small Angle, X-Rays
Stress25791003Modeling the behavior of Geobacillus stearothermophilus ATCC 12980 throughout its life cycle as vegetative cells or spores using growth boundaries.Mtimet N, Trunet C, Mathot AG, Venaille L, Leguerinel I, Coroller L, Couvert OFood Microbiol10.1016/j.fm.2014.10.0132014Colony Count, Microbial, Geobacillus stearothermophilus/chemistry/*growth & development, Hot Temperature, Hydrogen-Ion Concentration, Models, Theoretical, Spores, Bacterial/chemistry/growth & development
Biotechnology26919821Die another day: Fate of heat-treated Geobacillus stearothermophilus ATCC 12980 spores during storage under growth-preventing conditions.Mtimet N, Trunet C, Mathot AG, Venaille L, Leguerinel I, Coroller L, Couvert OFood Microbiol10.1016/j.fm.2015.12.0072015Food Contamination/*prevention & control, *Food Microbiology, Geobacillus stearothermophilus/*physiology, *Hot Temperature, Hydrogen-Ion Concentration, Microbial Viability, Models, Biological, Spores, Bacterial/growth & development/*physiology, Sterilization/methodsStress
Stress28213016Walking dead: Permeabilization of heat-treated Geobacillus stearothermophilus ATCC 12980 spores under growth-preventing conditions.Mtimet N, Trunet C, Mathot AG, Venaille L, Leguerinel I, Coroller L, Couvert OFood Microbiol10.1016/j.fm.2016.12.0132016Colony Count, Microbial, Geobacillus stearothermophilus/growth & development/*physiology, *Hot Temperature, Hydrogen-Ion Concentration, Linear Models, Microbial Viability, Models, Biological, Permeability, Spores, Bacterial/growth & development/*physiology
Enzymology30504002Heat resistance of spores of 18 strains of Geobacillus stearothermophilus and impact of culturing conditions.Wells-Bennik MHJ, Janssen PWM, Klaus V, Yang C, Zwietering MH, Den Besten HMWInt J Food Microbiol10.1016/j.ijfoodmicro.2018.11.0052018Colony Count, Microbial, Culture Media/chemistry, Geobacillus stearothermophilus/*isolation & purification, *Hot Temperature, Models, Theoretical, Spores, Bacterial/*isolation & purification, Steam, SterilizationPhylogeny
Biotechnology30934519Production of PR Toxin and Roquefortine by Penicillium roqueforti Isolates from Cabrales Blue Cheese.Medina M, Gaya P, Nunez MJ Food Prot10.4315/0362-028X-48.2.1181985
Stress31113104Recovery of Heat-stressed Spores of Bacillus stearothermophilus on Solid Media Containing Calcium- and Magnesium-deficient Agar.Sikes A, Whitfield S, Rosano DJJ Food Prot10.4315/0362-028X-56.8.7061993
31137427D Values of Bacillus stearothermophilus Spores as a Function of pH and Recovery Medium Acidulant.Fernandez PS, Gomez FJ, Ocio MJ, Rodrigo M, Sanchez T, Martinez AJ Food Prot10.4315/0362-028X-58.6.6281995
31195513Thermal Resistance of Bacillus stearothermophilus Heated at High Temperatures in Different Substrates.Rodrigo F, Fernandez PS, Rodrigo M, Ocio MJ, Martinez AJ Food Prot10.4315/0362-028X-60.2.1441997
Stress31520893The effect of phosphate on the heat resistance of spores of dairy isolates of Geobacillus stearothermophilus.Kumar M, Flint SH, Palmer J, Plieger PG, Waterland MInt J Food Microbiol10.1016/j.ijfoodmicro.2019.1083342019Dairying/methods, Disinfection/*methods, Geobacillus stearothermophilus/*growth & development, Heating/methods, Hot Temperature, Phosphates/*pharmacology, Picolinic Acids/*analysis, Spores, Bacterial/*drug effectsPathogenicity
Pathogenicity31521351A microbiological inhibition method for the rapid, broad-spectrum, and high-throughput screening of 34 antibiotic residues in milk.Wu Q, Zhu Q, Liu Y, Shabbir MAB, Sattar A, Peng D, Tao Y, Chen D, Wang Y, Yuan ZJ Dairy Sci10.3168/jds.2019-164802019Aminoglycosides/analysis, Animals, Anti-Bacterial Agents/*analysis, Cattle, Chromatography, Liquid/methods, Drug Residues/analysis, Food Contamination/*analysis, Geobacillus stearothermophilus/*drug effects, Macrolides/analysis, Milk/*chemistry, Reproducibility of Results, Sensitivity and Specificity, Tetracyclines/analysis
Cultivation32858380The search for a microbiological inhibition method for the rapid, broad-spectrum and high-throughput screening of six kinds of antibiotic residues in swine urine.Wu Q, Zhu Q, Shabbir MAB, Sattar A, Peng D, Tao Y, Chen D, Yuan Z, Wang YFood Chem10.1016/j.foodchem.2020.1275802020Aminoglycosides/pharmacology/urine, Animals, Anti-Bacterial Agents/analysis/pharmacology/*urine, Culture Media, Drug Residues/*analysis, False Negative Reactions, False Positive Reactions, Food Contamination/analysis, Geobacillus stearothermophilus/drug effects, High-Throughput Screening Assays/*methods, Limit of Detection, Macrolides/pharmacology/urine, Sensitivity and Specificity, Sulfonamides/pharmacology/urine, Swine, Tetracyclines/pharmacology/urine, Veterinary Drugs/pharmacology/*urinePathogenicity
Enzymology32922127Simultaneous production of alpha and beta amylase enzymes using separate gene bearing recombinant vectors in the same Escherichia coli cells.Ozcan D, SIpahIoGlu HMTurk J Biol10.3906/biy-2001-712020Biotechnology
33547059Influence of the incubation temperature and total dissolved solids concentration on the biofilm and spore formation of dairy isolates of Geobacillus stearothermophilus.Kumar M, Flint S, Palmer J, Chanapha S, Hall CAppl Environ Microbiol10.1128/AEM.02311-202021
Pathogenicity33783121Performance evaluation of bactericidal effect and endotoxin inactivation by low-temperature ozone/hydrogen peroxide mixed gas exposure.Nomura Y, Yamamura J, Fukui C, Fujimaki H, Sakamoto K, Matsuo KI, Kuromatsu H, Kikuchi Y, Haishima YJ Biomed Mater Res B Appl Biomater10.1002/jbm.b.348402021*Disinfection, Endotoxins/*chemistry, Escherichia coli/chemistry, Ethylene Oxide/*chemistry, Geobacillus stearothermophilus/chemistry, Humans, Hydrogen Peroxide/*chemistry

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