Strain identifier

BacDive ID: 14153

Type strain: Yes

Species: Novosphingobium lentum

Strain Designation: MT1

Strain history: CIP <- 2005, CCUG <- 2001, M.A. Tiirola, Jyväskylä, Finland: strain MT1

NCBI tax ID(s): 145287 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 4945

BacDive-ID: 14153

DSM-Number: 13663

keywords: 16S sequence, Bacteria, aerobe, psychrophilic, Gram-negative, rod-shaped

description: Novosphingobium lentum MT1 is an aerobe, psychrophilic, Gram-negative bacterium that was isolated from bioreactor prifying polychlorophenol contaminated graoundwater.

NCBI tax id

  • NCBI tax id: 145287
  • Matching level: species

strain history

@refhistory
4945<- M. Tiirola; MT1
120582CIP <- 2005, CCUG <- 2001, M.A. Tiirola, Jyväskylä, Finland: strain MT1

doi: 10.13145/bacdive14153.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Sphingomonadales
  • family: Erythrobacteraceae
  • genus: Novosphingobium
  • species: Novosphingobium lentum
  • full scientific name: Novosphingobium lentum Tiirola et al. 2005

@ref: 4945

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Sphingomonadales

family: Erythrobacteraceae

genus: Novosphingobium

species: Novosphingobium lentum

full scientific name: Novosphingobium lentum Tiirola et al. 2005

strain designation: MT1

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
31354negative1.05 µm0.4 µmrod-shapedno
120582negativerod-shapedno

pigmentation

  • @ref: 31354
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4945REACTIVATION WITH LIQUID MEDIUM 830 (DSMZ Medium 830c)yeshttps://mediadive.dsmz.de/medium/830cName: REACTIVATION WITH LIQUID MEDIUM 830 (DSMZ Medium 830c) Composition: Agar 15.0 g/l Yeast extract 0.5 g/l Proteose peptone 0.5 g/l Casamino acids 0.5 g/l Glucose 0.5 g/l Starch 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
33263MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromatiyesDistilled water make up to (1000.000 ml);R2A agar (18.200 g)
120582CIP Medium 566yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=566

culture temp

@refgrowthtypetemperaturerange
4945positivegrowth20psychrophilic
31354positivegrowth04-30
31354positiveoptimum23psychrophilic
33263positivegrowth22psychrophilic
56947positivegrowth20-26
120582positivegrowth22-30

Physiology and metabolism

oxygen tolerance

  • @ref: 31354
  • oxygen tolerance: aerobe

spore formation

  • @ref: 31354
  • spore formation: no

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3135416449alanine+carbon source
3135417632nitrate+reduction
6836925115malate-assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
12058217632nitrate+reduction
12058216301nitrite-reduction
12058217632nitrate-respiration

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
31354catalase+1.11.1.6
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
120582oxidase+
120582alcohol dehydrogenase-1.1.1.1
120582catalase+1.11.1.6
120582lysine decarboxylase-4.1.1.18
120582ornithine decarboxylase-4.1.1.17
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120582-+++-++-+-++---++---

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
4945--------------------+
4945--------------------+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling dateisolation date
4945bioreactor prifying polychlorophenol contaminated graoundwaterKärköläFinlandFINEurope
56947Sludge fed to fluidized-bed bioreactorKärköläFinlandFINEurope1999
120582Environment, Sludge fedKärköläFinlandFINEurope1999

isolation source categories

Cat1Cat2Cat3
#Engineered#Bioreactor
#Engineered#Contamination
#Environmental#Aquatic#Groundwater

taxonmaps

  • @ref: 69479
  • File name: preview.99_5359.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_20;96_437;97_3183;98_3997;99_5359&stattab=map
  • Last taxonomy: Novosphingobium lentum subclade
  • 16S sequence: AJ303009
  • Sequence Identity:
  • Total samples: 1100
  • soil counts: 288
  • aquatic counts: 573
  • animal counts: 176
  • plant counts: 63

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
49451Risk group (German classification)
1205821Risk group (French classification)

Sequence information

16S sequences

  • @ref: 4945
  • description: Sphingomonas sp. MT1 partial 16S rRNA gene
  • accession: AJ303009
  • length: 1440
  • database: ena
  • NCBI tax ID: 1219033

GC content

  • @ref: 4945
  • GC-content: 66

External links

@ref: 4945

culture collection no.: DSM 13663, CCUG 45847, CIP 108698

straininfo link

  • @ref: 83319
  • straininfo: 129068

literature

  • topic: Phylogeny
  • Pubmed-ID: 15774628
  • title: Novosphingobium lentum sp. nov., a psychrotolerant bacterium from a polychlorophenol bioremediation process.
  • authors: Tiirola MA, Busse HJ, Kampfer P, Mannisto MK
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.63386-0
  • year: 2005
  • mesh: Bacterial Typing Techniques, Biodegradation, Environmental, Chlorophenols/*metabolism, *Cold Temperature, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Finland, Fresh Water/*microbiology, Genes, rRNA, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sphingomonadaceae/*classification/genetics/metabolism/physiology, Water Pollutants, Chemical/*metabolism
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
4945Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13663)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13663
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31354Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2766728776041
33263Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6356
56947Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 45847)https://www.ccug.se/strain?id=45847
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
83319Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID129068.1StrainInfo: A central database for resolving microbial strain identifiers
120582Curators of the CIPCollection of Institut Pasteur (CIP 108698)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108698