Strain identifier
BacDive ID: 14142
Type strain: 
Species: Blastomonas ursincola
Strain Designation: KR-99, EY 4250
Strain history: CIP <- 2000, E. Yabuuchi, Osaka, Japan, strain EY 4250 <- DSMZ <- V.M. Gorlenko <- V. Yurkov: strain KR-99, Erythromicrobium ursincola
NCBI tax ID(s): 56361 (species)
version 8.1 (current version)
General
@ref: 3460
BacDive-ID: 14142
DSM-Number: 9006
keywords: 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, motile, rod-shaped
description: Blastomonas ursincola KR-99 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from freshwater cyanobacterial mat developing in the thermal springs at 34 to 40°C.
NCBI tax id
- NCBI tax id: 56361
- Matching level: species
strain history
| @ref | history |
|---|---|
| 3460 | <- V.M. Gorlenko <- V. Yurkov, KR-99 (Erythromicrobium ursincola) |
| 67770 | DSM 9006 <-- V. M. Gorlenko <-- V. Yurkov KR-99. |
| 123757 | CIP <- 2000, E. Yabuuchi, Osaka, Japan, strain EY 4250 <- DSMZ <- V.M. Gorlenko <- V. Yurkov: strain KR-99, Erythromicrobium ursincola |
doi: 10.13145/bacdive14142.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Sphingomonadales
- family: Sphingomonadaceae
- genus: Blastomonas
- species: Blastomonas ursincola
- full scientific name: Blastomonas ursincola (Yurkov et al. 1997) Hiraishi et al. 2000
synonyms
@ref synonym 20215 Erythromicrobium ursincola 20215 Erythromonas ursincola 20215 Sphingomonas ursincola
@ref: 3460
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Sphingomonadales
family: Sphingomonadaceae
genus: Blastomonas
species: Blastomonas ursincola
full scientific name: Blastomonas ursincola (Yurkov et al. 1997) Hiraishi et al. 2000
strain designation: KR-99, EY 4250
type strain: yes
Morphology
cell morphology
- @ref: 123757
- gram stain: negative
- cell shape: rod-shaped
- motility: yes
pigmentation
- @ref: 123757
- production: no
- name: Pyocyanin
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 3460 | PYEA MEDIUM (DSMZ Medium 364) | yes | https://mediadive.dsmz.de/medium/364 | Name: PYEA MEDIUM (DSMZ Medium 364) Composition: Agar 15.0 g/l Yeast extract 10.0 g/l Peptone 10.0 g/l NaCl 5.0 g/l Distilled water |
| 3460 | NUTRIENT AGAR (DSMZ Medium 1) | yes | https://mediadive.dsmz.de/medium/1 | Name: NUTRIENT AGAR (DSMZ Medium 1; with strain-specific modifications) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Na-acetate 1.0 g/l Distilled water |
| 33011 | MEDIUM 72- for trypto casein soja agar | yes | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |
| 123757 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 3460 | positive | growth | 26 | mesophilic |
| 33011 | positive | growth | 25 | mesophilic |
| 67770 | positive | growth | 26 | mesophilic |
| 123757 | positive | growth | 25-37 | mesophilic |
| 123757 | no | growth | 5 | psychrophilic |
| 123757 | no | growth | 10 | psychrophilic |
| 123757 | no | growth | 41 | thermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 123757
- oxygen tolerance: obligate aerobe
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 123757 | NaCl | positive | growth | 0-6 % |
| 123757 | NaCl | no | growth | 8 % |
| 123757 | NaCl | no | growth | 10 % |
observation
- @ref: 67770
- observation: quinones: Q-10
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 123757 | 4853 | esculin | - | hydrolysis |
| 123757 | 17632 | nitrate | - | reduction |
| 123757 | 16301 | nitrite | - | reduction |
| 123757 | 17632 | nitrate | - | respiration |
antibiotic resistance
- @ref: 123757
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: no
- is resistant: yes
metabolite production
- @ref: 123757
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 123757 | oxidase | + | |
| 123757 | beta-galactosidase | + | 3.2.1.23 |
| 123757 | alcohol dehydrogenase | - | 1.1.1.1 |
| 123757 | gelatinase | - | |
| 123757 | amylase | - | |
| 123757 | DNase | - | |
| 123757 | caseinase | - | 3.4.21.50 |
| 123757 | catalase | + | 1.11.1.6 |
| 123757 | tween esterase | + | |
| 123757 | lecithinase | - | |
| 123757 | lipase | - | |
| 123757 | lysine decarboxylase | - | 4.1.1.18 |
| 123757 | ornithine decarboxylase | - | 4.1.1.17 |
| 123757 | protease | - | |
| 123757 | tryptophan deaminase | - | |
| 123757 | urease | - | 3.5.1.5 |
API biotype100
| @ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 123757 | + | - | - | - | - | - | - | + | - | - | + | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | + | - |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 3460 | freshwater cyanobacterial mat developing in the thermal springs at 34 to 40°C | Kamchatka Island | Russia | RUS | Europe |
| 67770 | Freshwater cyanobacterial mat | ||||
| 123757 | Cyanobacterial mat |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Microbial | #Bacteria |
| #Environmental | #Aquatic | #Freshwater |
| #Environmental | #Microbial community | #Microbial mat |
| #Environmental | #Aquatic | #Thermal spring |
taxonmaps
- @ref: 69479
- File name: preview.99_993.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_20;96_574;97_668;98_775;99_993&stattab=map
- Last taxonomy: Blastomonas
- 16S sequence: Y10677
- Sequence Identity:
- Total samples: 53618
- soil counts: 10685
- aquatic counts: 21935
- animal counts: 16529
- plant counts: 4469
Safety information
risk assessment
| @ref | biosafety level | biosafety level comment |
|---|---|---|
| 3460 | 1 | Risk group (German classification) |
| 123757 | 1 | Risk group (French classification) |
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Erythromonas ursincola gene for 16S ribosomal RNA | AB024289 | 1476 | ena | 56361 |
| 20218 | E.ursincola 16S rRNA gene, partial | Y10677 | 1430 | ena | 56361 |
GC content
- @ref: 67770
- GC-content: 65.4
- method: thermal denaturation, midpoint method (Tm)
External links
@ref: 3460
culture collection no.: DSM 9006, JCM 13370, CIP 106843, JCM 10397
straininfo link
- @ref: 83307
- straininfo: 44208
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 10385200 | Proposal of Sphingomonas suberifaciens (van Bruggen, Jochimsen and Brown 1990) comb. nov., sphingomonas natatoria (Sly 1985) comb. nov., Sphingomonas ursincola (Yurkov et al. 1997) comb. nov., and emendation of the genus Sphingomonas. | Yabuuchi E, Kosako Y, Naka T, Suzuki S, Yano I | Microbiol Immunol | 10.1111/j.1348-0421.1999.tb02414.x | 1999 | Base Composition, DNA, Bacterial, DNA, Ribosomal, Genotype, Glycosphingolipids/analysis, Gram-Negative Aerobic Bacteria/chemistry/*classification/genetics, Microbial Sensitivity Tests, Phylogeny, RNA, Ribosomal, 16S | Genetics |
| Phylogeny | 10843052 | Emendation of the description of Blastomonas natatoria (Sly 1985) Sly and Cahill 1997 as an aerobic photosynthetic bacterium and reclassification of Erythromonas ursincola Yurkov et al. 1997 as Blastomonas ursincola comb. nov. | Hiraishi A, Kuraishi H, Kawahara K | Int J Syst Evol Microbiol | 10.1099/00207713-50-3-1113 | 2000 | Alphaproteobacteria/chemistry/*classification/genetics/metabolism, Bacterial Proteins/genetics, Bacteriochlorophylls/analysis, DNA, Ribosomal/analysis/genetics, Gram-Negative Aerobic Bacteria/chemistry/*classification/genetics/metabolism, Light, Light-Harvesting Protein Complexes, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, *Photosynthesis, Photosynthetic Reaction Center Complex Proteins/genetics, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Metabolism |
| Phylogeny | 25724744 | Blastomonas aquatica sp. nov., a bacteriochlorophyll-containing bacterium isolated from lake water. | Xiao N, Liu Y, Liu X, Gu Z, Jiao N, Liu H, Zhou Y, Shen L | Int J Syst Evol Microbiol | 10.1099/ijs.0.000153 | 2015 | Bacterial Typing Techniques, Bacteriochlorophylls/*chemistry, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fresh Water, Lakes/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sphingomonadaceae/*classification/genetics/isolation & purification, Ubiquinone/analogs & derivatives/chemistry | Genetics |
| Phylogeny | 26613616 | Binariimonas pacifica gen. nov., sp. nov., a Novel Marine Bacterium of Family Sphingomonadaceae Isolated from East Pacific Ocean Surface Seawater. | Zhao Z, Sun J, Zhang R, Jiao N | Curr Microbiol | 10.1007/s00284-015-0948-x | 2015 | Aquatic Organisms/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Glycolipids/analysis, Microscopy, Electron, Transmission, Molecular Sequence Data, Pacific Ocean, Phospholipids/analysis, Phylogeny, Pigments, Biological/analysis, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Sphingomonadaceae/*classification/genetics/*isolation & purification/physiology | Enzymology |
| Phylogeny | 28631592 | Blastomonas quesadae sp. nov., isolated from a saline soil by dilution-to-extinction cultivation. | Castro DJ, Llamas I, Bejar V, Martinez-Checa F | Int J Syst Evol Microbiol | 10.1099/ijsem.0.001902 | 2017 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Salinity, Sequence Analysis, DNA, *Soil Microbiology, Spain, Sphingomonadaceae/*classification/genetics/isolation & purification, Ubiquinone/chemistry | Transcriptome |
| Phylogeny | 28820119 | Blastomonas fulva sp. nov., aerobic photosynthetic bacteria isolated from a Microcystis culture. | Lee HG, Ko SR, Lee JW, Lee CS, Ahn CY, Oh HM, Jin L | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002084 | 2017 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Microcystis, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Sphingomonadaceae/*classification/genetics/isolation & purification, Ubiquinone/chemistry | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 3460 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 9006) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-9006 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 33011 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/19018 | ||||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 83307 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID44208.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
| 123757 | Curators of the CIP | Collection of Institut Pasteur (CIP 106843) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106843 |