Strain identifier
BacDive ID: 141169
Type strain:
Species: Micromonospora grosourdyae
Strain Designation: A-T 7972
Strain history: <- TBRC; TBRC 6031
NCBI tax ID(s): 2047739 (species)
General
@ref: 44089
BacDive-ID: 141169
DSM-Number: 114116
keywords: 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive
description: Micromonospora grosourdyae A-T 7972 is an aerobe, spore-forming, Gram-positive bacterium that builds a substrate mycelium and was isolated from from wild orchid Grosourdya appendiculata.
NCBI tax id
- NCBI tax id: 2047739
- Matching level: species
strain history
- @ref: 69026
- history: <- TBRC; TBRC 6031
doi: 10.13145/bacdive141169.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Micromonosporales
- family: Micromonosporaceae
- genus: Micromonospora
- species: Micromonospora grosourdyae
- full scientific name: Micromonospora grosourdyae Carro and Oren 2022
synonyms
- @ref: 20215
- synonym: Verrucosispora endophytica
@ref: 44089
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Micromonosporales
family: Micromonosporaceae
genus: Verrucosispora
species: Verrucosispora endophytica
full scientific name: Verrucosispora endophytica Ngaemthao et al. 2017
strain designation: A-T 7972
type strain: yes
Morphology
cell morphology
- @ref: 44089
- gram stain: positive
colony morphology
- @ref: 44089
- colony color: orange
- medium used: ISP 2
multicellular morphology
- @ref: 44089
- forms multicellular complex: yes
- complex name: substrate mycelium
multimedia
- @ref: 69026
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_114116.jpg
- caption: Medium 65, 28°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
@ref | name | growth | link |
---|---|---|---|
69026 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium65.pdf |
44089 | ISP 2 | yes | |
44089 | ISP 3 | yes | |
44089 | ISP 7 | yes |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
44089 | positive | growth | 20-37 | |
44089 | positive | optimum | 25-30 | mesophilic |
69026 | positive | growth | 28 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
44089 | positive | growth | 7-11 | alkaliphile |
44089 | positive | optimum | 9-10 |
Physiology and metabolism
oxygen tolerance
- @ref: 44089
- oxygen tolerance: aerobe
spore formation
- @ref: 44089
- spore description: single non-motile spores with warty surface formed at the tip of sporophores on substrate mycelia
- spore formation: yes
halophily
- @ref: 44089
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0-3 %(w/v)
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
44089 | 12936 | D-galactose | - | assimilation |
44089 | 16988 | D-ribose | - | assimilation |
44089 | 17924 | D-sorbitol | - | assimilation |
44089 | 17151 | xylitol | - | assimilation |
44089 | 18403 | L-arabitol | - | assimilation |
44089 | 17266 | L-sorbose | - | assimilation |
44089 | 17716 | lactose | - | assimilation |
44089 | 16634 | raffinose | - | assimilation |
44089 | 17368 | hypoxanthine | - | hydrolysis |
44089 | 15318 | xanthine | - | hydrolysis |
44089 | 17632 | nitrate | - | reduction |
44089 | 15824 | D-fructose | + | assimilation |
44089 | 17634 | D-glucose | + | assimilation |
44089 | 16899 | D-mannitol | + | assimilation |
44089 | 16024 | D-mannose | + | assimilation |
44089 | 65327 | D-xylose | + | assimilation |
44089 | 17268 | myo-inositol | + | assimilation |
44089 | 30849 | L-arabinose | + | assimilation |
44089 | 62345 | L-rhamnose | + | assimilation |
44089 | 17306 | maltose | + | assimilation |
44089 | 17992 | sucrose | + | assimilation |
44089 | 27082 | trehalose | + | assimilation |
44089 | 28017 | starch | + | hydrolysis |
enzymes
@ref | value | activity | ec |
---|---|---|---|
44089 | esterase (C 4) | + | |
44089 | leucine arylamidase | + | 3.4.11.1 |
44089 | trypsin | + | 3.4.21.4 |
44089 | alpha-chymotrypsin | + | 3.4.21.1 |
44089 | alpha-glucosidase | + | 3.2.1.20 |
44089 | beta-glucosidase | + | 3.2.1.21 |
44089 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
44089 | catalase | + | 1.11.1.6 |
fatty acid profile
fatty acids
@ref fatty acid percentage 44089 10-methyl C18:0 TBSA 0.17 44089 C15:0 anteiso 5.02 44089 C17:0 anteiso 6.92 44089 C17:1 anteiso ω9c 2 44089 C12:0 0.08 44089 C14:0 0.15 44089 C15:1ω6c 0.27 44089 C16:0 1.39 44089 C16:0 2OH 0.16 44089 C17:0 1.39 44089 C17:0 3OH 0.07 44089 C17:1ω8c 9.1 44089 C18:0 1.14 44089 C18:1 2OH 0.12 44089 C18:1ω9c 3.02 44089 C19:0 0.04 44089 C14:0 iso 0.88 44089 C15:0 iso 18.37 44089 C16:0 iso 33.12 44089 C16:1 iso H 2.86 44089 C17:0 iso 3.15 44089 C18:0 iso 0.31 - type of FA analysis: whole cell analysis
- incubation medium: ISP 2
- incubation temperature: 30
- incubation time: 7
- library/peak naming table: RTSBA6
- system: MIS MIDI
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | enrichment culture | enrichment culture composition | enrichment culture duration | enrichment culture temperature | isolation procedure |
---|---|---|---|---|---|---|---|---|---|---|
44089 | from wild orchid Grosourdya appendiculata | Nakhorn Ratchasima Province | Thailand | THA | Asia | humic acid-salts vitamin agar | supplemented with 50mg/l of nalidixic acid, 100mg/l of cycloheximide and 2mg/l of terbinafine | 21 days | 28 | The root was treated using surface sterilization technique by sequential immersion in 70% (v/v) ethanol for 5min and sodium hypochlorite solution (4% available chloride, freshly prepared) for 5min. Then the sample was washed with sterile distilled water three times to remove surface sterilization agents and soaked in 10% NaHCO3 solution for 10min to disrupt growth of endophytic fungi. One gram of sample was cut into small pieces with sterilized scissors and then crushed in 9ml sterile sodium dod |
69026 | Root of plant, Grosourdya appendiculata | Nakhorn Ratchasima | Thailand | THA | Asia |
isolation source categories
- Cat1: #Host
- Cat2: #Plants
- Cat3: #Herbaceous plants (Grass,Crops)
Safety information
risk assessment
- @ref: 69026
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 44089
- description: 16S rRNA gene sequence
- accession: MF033262
- database: nuccore
GC content
- @ref: 44089
- GC-content: 70.5
- method: high performance liquid chromatography (HPLC)
External links
@ref: 44089
culture collection no.: BCC 50981, TBRC 6031, NBRC 112512, DSM 114116
straininfo link
- @ref: 96877
- straininfo: 405167
literature
- topic: Phylogeny
- Pubmed-ID: 29043951
- title: Verrucosispora endophytica sp. nov., isolated from the root of wild orchid (Grosourdya appendiculata (Blume) Rchb.f.).
- authors: Ngaemthao W, Pujchakarn T, Chunhametha S, Suriyachadkun C
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.002425
- year: 2017
- mesh: Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Micromonosporaceae/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Orchidaceae/*microbiology, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, Plant Roots/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Thailand, Vitamin K 2/analogs & derivatives/chemistry
- topic2: Transcriptome
Reference
@id | authors | title | doi/url | journal | pubmed | catalogue |
---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
44089 | Wipaporn Ngaemthao, Tawanmol Pujchakarn, Suwanee Chunhametha and Chanwit Suriyachadkun | Verrucosispora endophytica sp. nov., isolated from the root of wild orchid (Grosourdya appendiculata (Blume) Rchb.f.) | 10.1099/ijsem.0.002425 | IJSEM 67: 5114-5119 2017 | 29043951 | |
69026 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-114116 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 114116) | |||
96877 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID405167.1 |