Strain identifier

BacDive ID: 141169

Type strain: Yes

Species: Micromonospora grosourdyae

Strain Designation: A-T 7972

Strain history: <- TBRC; TBRC 6031

NCBI tax ID(s): 2047739 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 44089

BacDive-ID: 141169

DSM-Number: 114116

keywords: 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive

description: Micromonospora grosourdyae A-T 7972 is an aerobe, spore-forming, Gram-positive bacterium that builds a substrate mycelium and was isolated from from wild orchid Grosourdya appendiculata.

NCBI tax id

  • NCBI tax id: 2047739
  • Matching level: species

strain history

  • @ref: 69026
  • history: <- TBRC; TBRC 6031

doi: 10.13145/bacdive141169.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micromonosporales
  • family: Micromonosporaceae
  • genus: Micromonospora
  • species: Micromonospora grosourdyae
  • full scientific name: Micromonospora grosourdyae Carro and Oren 2022
  • synonyms

    • @ref: 20215
    • synonym: Verrucosispora endophytica

@ref: 44089

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Micromonosporales

family: Micromonosporaceae

genus: Verrucosispora

species: Verrucosispora endophytica

full scientific name: Verrucosispora endophytica Ngaemthao et al. 2017

strain designation: A-T 7972

type strain: yes

Morphology

cell morphology

  • @ref: 44089
  • gram stain: positive

colony morphology

  • @ref: 44089
  • colony color: orange
  • medium used: ISP 2

multicellular morphology

  • @ref: 44089
  • forms multicellular complex: yes
  • complex name: substrate mycelium

multimedia

  • @ref: 69026
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_114116.jpg
  • caption: Medium 65, 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlink
69026GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium65.pdf
44089ISP 2yes
44089ISP 3yes
44089ISP 7yes

culture temp

@refgrowthtypetemperaturerange
44089positivegrowth20-37
44089positiveoptimum25-30mesophilic
69026positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
44089positivegrowth7-11alkaliphile
44089positiveoptimum9-10

Physiology and metabolism

oxygen tolerance

  • @ref: 44089
  • oxygen tolerance: aerobe

spore formation

  • @ref: 44089
  • spore description: single non-motile spores with warty surface formed at the tip of sporophores on substrate mycelia
  • spore formation: yes

halophily

  • @ref: 44089
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-3 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4408912936D-galactose-assimilation
4408916988D-ribose-assimilation
4408917924D-sorbitol-assimilation
4408917151xylitol-assimilation
4408918403L-arabitol-assimilation
4408917266L-sorbose-assimilation
4408917716lactose-assimilation
4408916634raffinose-assimilation
4408917368hypoxanthine-hydrolysis
4408915318xanthine-hydrolysis
4408917632nitrate-reduction
4408915824D-fructose+assimilation
4408917634D-glucose+assimilation
4408916899D-mannitol+assimilation
4408916024D-mannose+assimilation
4408965327D-xylose+assimilation
4408917268myo-inositol+assimilation
4408930849L-arabinose+assimilation
4408962345L-rhamnose+assimilation
4408917306maltose+assimilation
4408917992sucrose+assimilation
4408927082trehalose+assimilation
4408928017starch+hydrolysis

enzymes

@refvalueactivityec
44089esterase (C 4)+
44089leucine arylamidase+3.4.11.1
44089trypsin+3.4.21.4
44089alpha-chymotrypsin+3.4.21.1
44089alpha-glucosidase+3.2.1.20
44089beta-glucosidase+3.2.1.21
44089N-acetyl-beta-glucosaminidase+3.2.1.52
44089catalase+1.11.1.6

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    4408910-methyl C18:0 TBSA0.17
    44089C15:0 anteiso5.02
    44089C17:0 anteiso6.92
    44089C17:1 anteiso ω9c2
    44089C12:00.08
    44089C14:00.15
    44089C15:1ω6c0.27
    44089C16:01.39
    44089C16:0 2OH0.16
    44089C17:01.39
    44089C17:0 3OH0.07
    44089C17:1ω8c9.1
    44089C18:01.14
    44089C18:1 2OH0.12
    44089C18:1ω9c3.02
    44089C19:00.04
    44089C14:0 iso0.88
    44089C15:0 iso18.37
    44089C16:0 iso33.12
    44089C16:1 iso H2.86
    44089C17:0 iso3.15
    44089C18:0 iso0.31
  • type of FA analysis: whole cell analysis
  • incubation medium: ISP 2
  • incubation temperature: 30
  • incubation time: 7
  • library/peak naming table: RTSBA6
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperatureisolation procedure
44089from wild orchid Grosourdya appendiculataNakhorn Ratchasima ProvinceThailandTHAAsiahumic acid-salts vitamin agarsupplemented with 50mg/l of nalidixic acid, 100mg/l of cycloheximide and 2mg/l of terbinafine21 days28The root was treated using surface sterilization technique by sequential immersion in 70% (v/v) ethanol for 5min and sodium hypochlorite solution (4% available chloride, freshly prepared) for 5min. Then the sample was washed with sterile distilled water three times to remove surface sterilization agents and soaked in 10% NaHCO3 solution for 10min to disrupt growth of endophytic fungi. One gram of sample was cut into small pieces with sterilized scissors and then crushed in 9ml sterile sodium dod
69026Root of plant, Grosourdya appendiculataNakhorn RatchasimaThailandTHAAsia

isolation source categories

  • Cat1: #Host
  • Cat2: #Plants
  • Cat3: #Herbaceous plants (Grass,Crops)

Safety information

risk assessment

  • @ref: 69026
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 44089
  • description: 16S rRNA gene sequence
  • accession: MF033262
  • database: nuccore

GC content

  • @ref: 44089
  • GC-content: 70.5
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 44089

culture collection no.: BCC 50981, TBRC 6031, NBRC 112512, DSM 114116

straininfo link

  • @ref: 96877
  • straininfo: 405167

literature

  • topic: Phylogeny
  • Pubmed-ID: 29043951
  • title: Verrucosispora endophytica sp. nov., isolated from the root of wild orchid (Grosourdya appendiculata (Blume) Rchb.f.).
  • authors: Ngaemthao W, Pujchakarn T, Chunhametha S, Suriyachadkun C
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002425
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Micromonosporaceae/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Orchidaceae/*microbiology, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, Plant Roots/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Thailand, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
44089Wipaporn Ngaemthao, Tawanmol Pujchakarn, Suwanee Chunhametha and Chanwit SuriyachadkunVerrucosispora endophytica sp. nov., isolated from the root of wild orchid (Grosourdya appendiculata (Blume) Rchb.f.)10.1099/ijsem.0.002425IJSEM 67: 5114-5119 201729043951
69026Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-114116Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 114116)
96877Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID405167.1