Strain identifier

BacDive ID: 141136

Type strain: Yes

Species: Halorubrum halodurans

NCBI tax ID(s): 1383851 (species)

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General

@ref: 44052

BacDive-ID: 141136

keywords: 16S sequence, Archaea, obligate aerobe, chemoorganotroph, Gram-variable, motile, rod-shaped, colony-forming, pigmented

description: Halorubrum halodurans CECT 8745 is an obligate aerobe, chemoorganotroph, Gram-variable archaeon that forms circular colonies and has a pink to red pigmentation.

NCBI tax id

  • NCBI tax id: 1383851
  • Matching level: species

doi: 10.13145/bacdive141136.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Halobacteria
  • order: Haloferacales
  • family: Halorubraceae
  • genus: Halorubrum
  • species: Halorubrum halodurans
  • full scientific name: Halorubrum halodurans Corral et al. 2016

@ref: 44052

domain: Archaea

phylum: Euryarchaeota

class: Halobacteria

order: Haloferacales

family: Halorubraceae

genus: Halorubrum

species: Halorubrum halodurans

type strain: yes

Morphology

cell morphology

  • @ref: 44052
  • gram stain: variable
  • cell length: 5.0-10 µm
  • cell width: 1.0-4.0 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 44052
  • colony size: 0.5-1 mm
  • colony color: pink-red
  • colony shape: circular
  • incubation period: 10 days
  • medium used: solid medium

pigmentation

  • @ref: 44052
  • production: yes
  • color: pink to red
  • name: bacterioruberin carotenoid

Culture and growth conditions

culture medium

@refnamegrowthcomposition
44052M1 20 % (Rodríguez-Valera et al., 1980)yesStock Solution SW 30 % (w/v, Subow, 1931) consisting of (per litre): 234 g NaCl, 39 g MgCl2 . 6H2O, 61 g MgSO4 . 7H2O, 1 g CaCl2 , 6 g KCl, 0.2 g NaHCO3 and 0.7 g NaBr, supplemented with 0.2 % (w/v) yeast extract, 0.1 % (w/v) Casamino acids and 0.005 % (w/v) sodium pyruvate; adjusted to pH 7.0–7.2. For solid media, 2.0 % (w/v) agar.
44052solid mediumyes

culture temp

@refgrowthtypetemperature
44052positivegrowth20-45
44052positiveoptimum37

culture pH

@refabilitytypepH
44052positivegrowth6.0-8.0
44052positiveoptimum7.0-7.2

Physiology and metabolism

oxygen tolerance

  • @ref: 44052
  • oxygen tolerance: obligate aerobe

nutrition type

  • @ref: 44052
  • type: chemoorganotroph

halophily

@refsaltgrowthtested relationconcentrationhalophily level
44052MgCl2positiveminimum0.3 M
44052NaClpositivegrowth20-30 %(w/v)
44052NaClpositiveoptimum25 %(w/v)extremely halophilic

observation

  • @ref: 44052
  • observation: hypotonic treatment with less than 10 % (w/v) NaCl induces cell lysis

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4405216449alanine-carbon source
4405229016arginine-carbon source
4405216947citrate-carbon source
4405215824D-fructose-carbon source
4405212936D-galactose-carbon source
4405216988D-ribose-carbon source
4405265327D-xylose-carbon source
4405229806fumarate-carbon source
4405224898isoleucine-carbon source
4405230849L-arabinose-carbon source
4405217716lactose-carbon source
4405217306maltose-carbon source
4405218257ornithine-carbon source
4405216634raffinose-carbon source
4405226546rhamnose-carbon source
4405230911sorbitol-carbon source
4405227922sorbose-carbon source
4405216449alanine-energy source
4405229016arginine-energy source
4405216947citrate-energy source
4405215824D-fructose-energy source
4405212936D-galactose-energy source
4405216988D-ribose-energy source
4405265327D-xylose-energy source
4405229806fumarate-energy source
4405224898isoleucine-energy source
4405230849L-arabinose-energy source
4405217716lactose-energy source
4405217306maltose-energy source
4405218257ornithine-energy source
4405216634raffinose-energy source
4405226546rhamnose-energy source
4405230911sorbitol-energy source
4405227922sorbose-energy source
4405217234glucose-fermentation
44052casein-hydrolysis
4405216991dna-hydrolysis
440525291gelatin-hydrolysis
4405228017starch-hydrolysis
4405253426tween 80-hydrolysis
4405216449alanine-nitrogen source
4405229016arginine-nitrogen source
4405224898isoleucine-nitrogen source
4405218257ornithine-nitrogen source
4405216301nitrite-reduction
4405230089acetate+carbon source
4405222653asparagine+carbon source
4405217634D-glucose+carbon source
4405216899D-mannitol+carbon source
4405216024D-mannose+carbon source
4405229987glutamate+carbon source
4405217754glycerol+carbon source
4405215428glycine+carbon source
4405218019L-lysine+carbon source
4405217115L-serine+carbon source
4405224996lactate+carbon source
4405225115malate+carbon source
4405217272propionate+carbon source
4405215361pyruvate+carbon source
4405230031succinate+carbon source
4405217992sucrose+carbon source
4405226986threonine+carbon source
4405227082trehalose+carbon source
4405230089acetate+energy source
4405222653asparagine+energy source
4405217634D-glucose+energy source
4405216899D-mannitol+energy source
4405216024D-mannose+energy source
4405229987glutamate+energy source
4405217754glycerol+energy source
4405215428glycine+energy source
4405218019L-lysine+energy source
4405217115L-serine+energy source
4405224996lactate+energy source
4405225115malate+energy source
4405217272propionate+energy source
4405215361pyruvate+energy source
4405230031succinate+energy source
4405217992sucrose+energy source
4405226986threonine+energy source
4405227082trehalose+energy source
4405222653asparagine+nitrogen source
4405215428glycine+nitrogen source
4405218019L-lysine+nitrogen source
4405217115L-serine+nitrogen source
4405226986threonine+nitrogen source
4405217632nitrate+reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.group IDis resistantresistance conc.
44052338412(-)-anisomycinyesyes50 µg (disc)
4405228669bacitracinyesyes10 Unit (disc)
4405228368novobiocinyesyes30 µg (disc)
4405228077rifampicinyesyes5 µg (disc)
4405248923erythromycinyesyes15 µg (disc)
4405217076streptomycinyesyes10 µg (disc)
4405245924trimethoprimyesyes1.25 µg (disc)26
440529332sulfamethoxazoleyesyes23.75 µg (disc)26
4405228971ampicillinyesyes10 µg (disc)
4405217698chloramphenicolyesyes30 µg (disc)
44052100147nalidixic acidyesyes30 µg (disc)
440527507neomycinyesyes30 Unit (disc)
4405217833gentamicinyesyes10 Unit (disc)
440526104kanamycinyesyes30 µg (disc)
4405218208penicillin gyesyes10 Unit (disc)
4405227902tetracyclineyesyes30 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
4405215688acetoinno
4405235581indoleno
4405216136hydrogen sulfideyes

metabolite tests

@refChebi-IDmetabolitemethylred-testvoges-proskauer-testcitrate test
4405217234glucose-
4405215688acetoin-
4405216947citrate-

enzymes

@refvalueactivityec
44052cytochrome oxidase+1.9.3.1
44052catalase+1.11.1.6
44052arginine dihydrolase-3.5.3.6
44052lysine decarboxylase-4.1.1.18
44052ornithine decarboxylase-4.1.1.17
44052urease-3.5.1.5
44052phosphatase-

Isolation, sampling and environmental information

isolation

  • @ref: 44052
  • sample type: sediment brine of a hypersaline lake
  • geographic location: Lake Aran-Bidgol
  • country: Iran
  • origin.country: IRN
  • continent: Asia
  • enrichment culture: Hv-YPC solid medium
  • enrichment culture duration: 28 days
  • enrichment culture temperature: 37
  • isolation procedure: incubation aerobically in sealed plastic bags

isolation source categories

Cat1Cat2Cat3
#Condition#Saline
#Environmental#Aquatic#Lake (large)
#Environmental#Aquatic#Sediment

taxonmaps

  • @ref: 69479
  • File name: preview.99_192096.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17655;96_69974;97_92130;98_125215;99_192096&stattab=map
  • Last taxonomy: Halorubrum
  • 16S sequence: HG421007
  • Sequence Identity:
  • Total samples: 1
  • aquatic counts: 1

Sequence information

16S sequences

  • @ref: 44052
  • description: Halorubrum halodurans partial 16S rRNA gene, strain Cb34
  • accession: HG421007
  • database: nuccore

GC content

  • @ref: 44052
  • GC-content: 62.1
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 44052

culture collection no.: CECT 8745, IBRC-M 10233, CB Cb34

literature

  • topic: Phylogeny
  • Pubmed-ID: 26537912
  • title: Halorubrum halodurans sp. nov., an extremely halophilic archaeon isolated from a hypersaline lake.
  • authors: Corral P, de la Haba RR, Sanchez-Porro C, Ali Amoozegar M, Thane Papke R, Ventosa A
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.000738
  • year: 2015
  • mesh: Base Composition, DNA, Archaeal/genetics, Halorubrum/*classification/genetics/isolation & purification, Iran, Lakes/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Saline Waters, Sequence Analysis, DNA
  • topic2: Genetics

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
44052Paulina Corral, Rafael R. de la Haba, Cristina Sánchez-Porro, Mohammad Ali Amoozegar, R. Thane Papke and Antonio VentosaHalorubrum halodurans sp. nov., an extremely halophilic archaeon isolated from a hypersaline lake10.1099/ijsem.0.000738IJSEM 66: 435-444 201626537912
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/