Strain identifier

BacDive ID: 141133

Type strain: Yes

Species: Flavobacterium collinsense

Strain Designation: 4-T-2

NCBI tax ID(s): 1535303 (species)

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General

@ref: 44048

BacDive-ID: 141133

keywords: 16S sequence, Bacteria, aerobe, Gram-negative, rod-shaped, colony-forming

description: Flavobacterium collinsense 4-T-2 is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from till sample of a glacier front.

NCBI tax id

  • NCBI tax id: 1535303
  • Matching level: species

doi: 10.13145/bacdive141133.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Flavobacterium
  • species: Flavobacterium collinsense
  • full scientific name: Flavobacterium collinsense Zhang et al. 2016

@ref: 44048

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Flavobacterium

species: Flavobacterium collinsense

strain designation: 4-T-2

type strain: yes

Morphology

cell morphology

  • @ref: 44048
  • gram stain: negative
  • cell length: 0.5-1.0 µm
  • cell width: 0.2-0.3 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 44048
  • colony size: 0.5-1.0 mm
  • colony color: yellow
  • colony shape: circular
  • incubation period: 3 days
  • medium used: R2A agar

pigmentation

  • @ref: 44048
  • production: no
  • name: flexirubin-type

Culture and growth conditions

culture medium

@refnamegrowth
44048LB (Luria-Bertani) MEDIUMno
44048MBno
44048Nutrient agar (NA)yes
44048Reasoner's 2A agar (R2A)yes
44048TSB agaryes

culture temp

@refgrowthtypetemperature
44048positivegrowth4-28
44048positiveoptimum18-20

culture pH

@refabilitytypepHPH range
44048positivegrowth7.0-10.0alkaliphile
44048positiveoptimum9

Physiology and metabolism

oxygen tolerance

  • @ref: 44048
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentrationhalophily level
44048NaClpositivegrowth0-1.0 %(w/v)
44048NaClpositiveoptimum0 %(w/v)non-halophilic

observation

  • @ref: 44048
  • observation: surrounded by a multilayer capsule

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4404885146carboxymethylcellulose-hydrolysis
4404816235guanine-hydrolysis
4404817368hypoxanthine-hydrolysis
4404828017starch-hydrolysis
4404815318xanthine-hydrolysis
440484853esculin+builds acid from
4404828087glycogen+builds acid from
4404817716lactose+builds acid from
4404817306maltose+builds acid from
4404828017starch+builds acid from
4404816988D-ribose+carbon source
44048potassium 5-dehydro-D-gluconate+carbon source
44048casein+hydrolysis
4404853424tween 20+hydrolysis
4404853423tween 40+hydrolysis
4404853425tween 60+hydrolysis
4404853426tween 80+hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.
4404828971ampicillinyesyes10 µg (disc)
440486104kanamycinyesyes30 µg (disc)

metabolite production

  • @ref: 44048
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: no

enzymes

@refvalueactivityec
44048cytochrome oxidase+1.9.3.1
44048catalase-1.11.1.6
44048gelatinase+
44048beta-galactosidase+/-3.2.1.23
44048alkaline phosphatase+3.1.3.1
44048esterase Lipase (C 8)+
44048leucine arylamidase+3.4.11.1
44048valine arylamidase+
44048acid phosphatase+3.1.3.2
44048naphthol-AS-BI-phosphohydrolase+
44048esterase (C 4)+/-
44048cystine arylamidase+/-3.4.11.3
44048alpha-glucosidase+/-3.2.1.20
44048lipase (C 14)-
44048trypsin-3.4.21.4
44048alpha-chymotrypsin-3.4.21.1
44048alpha-galactosidase-3.2.1.22
44048beta-galactosidase-3.2.1.23
44048beta-glucuronidase-3.2.1.31
44048beta-glucosidase-3.2.1.21
44048N-acetyl-beta-glucosaminidase-3.2.1.52
44048alpha-mannosidase-3.2.1.24
44048beta-D-fucosidase-3.2.1.38

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    44048C15:0 anteiso5.9
    44048anteiso-C15:1-A1.9
    44048C17:1 anteiso ω9c1.4
    44048C15:1ω6c1.8
    44048C16:1ω7c / C16:1ω6c2.1
    44048C17:0 2OH2.9
    44048C17:1ω8c1.1
    44048C17:1ω9c / C16:0-10-methyl10
    44048C18:1ω5c2.2
    44048C14:0 iso1.5
    44048iso-C14:0-3-OH1
    44048C15:0 iso14.4
    44048iso-C15:0-3-OH5.6
    44048iso-C15:1-G10.7
    44048C16:0 iso13.9
    44048iso-C16:0-3-OH8.8
    44048iso-C16:1-H8.4
    44048iso-C17:0-3-OH2.4
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A
  • incubation temperature: 20
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • method/protocol: Sasser, 1990
  • cutoff value:

Isolation, sampling and environmental information

isolation

  • @ref: 44048
  • sample type: till sample of a glacier front
  • geographic location: Collins Glacier, King George Island
  • country: Antarctica
  • origin.country: ATA
  • continent: Australia and Oceania
  • latitude: -62.1823
  • longitude: -58.8667
  • enrichment culture: R2A
  • enrichment culture temperature: 4

isolation source categories

Cat1Cat2Cat3
#Climate#Cold#Polar
#Environmental#Terrestrial#Glacier

Sequence information

16S sequences

  • @ref: 44048
  • description: Flavobacterium collinsense 16S ribosomal RNA gene, partial sequence
  • accession: KM288594
  • database: nuccore

GC content

  • @ref: 44048
  • GC-content: 36.2
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 44048

culture collection no.: LMG 28257, CCTCC AB 2014004

straininfo link

  • @ref: 96867
  • straininfo: 393804

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny26475320Flavobacterium collinsense sp. nov., isolated from a till sample of an Antarctic glacier.Zhang Y, Jiang F, Chang X, Qiu X, Ren L, Qu Z, Deng S, Da X, Fang C, Peng FInt J Syst Evol Microbiol10.1099/ijsem.0.0006882015Antarctic Regions, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacterium/*classification/genetics/isolation & purification, Ice Cover/*microbiology, Molecular Sequence Data, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryGenetics
Phylogeny30022265Flavobacterium knui sp. nov., a novel member of the family Flavobacteriaceae.Kim SY, Ten LN, Lee SY, Kim SH, Kang IK, Jung HYAntonie Van Leeuwenhoek10.1007/s10482-018-1128-32018Bacterial Typing Techniques, Base Composition, DNA, Bacterial/*genetics, Fatty Acids/biosynthesis, Flavobacterium/chemistry/*classification/genetics/isolation & purification, Genotype, Phenotype, Phosphatidylethanolamines/biosynthesis, *Phylogeny, RNA, Ribosomal, 16S/*genetics, Republic of Korea, Water MicrobiologyGenetics

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
44048Yumin Zhang, Fan Jiang, Xulu Chang, Xia Qiu, Lvzhi Ren, Zhihao Qu, Sangsang Deng, Xuyang Da, Chengxiang Fang and Fang PengFlavobacterium collinsense sp. nov., isolated from a till sample of an Antarctic glacier10.1099/ijsem.0.000688IJSEM 66: 172-177 201626475320
96867Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID393804.1