Strain identifier
BacDive ID: 141107
Type strain: 
Species: Streptomyces amphotericinicus
Strain Designation: 1H-SSA8
Strain history: <- C. Liu, School of Life Science, Northeast Agricultural University; 1H-SSA8
NCBI tax ID(s): 2023875 (species)
version 8.1 (current version)
General
@ref: 64978
BacDive-ID: 141107
DSM-Number: 103128
keywords: 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive
description: Streptomyces amphotericinicus 1H-SSA8 is an aerobe, spore-forming, Gram-positive bacterium that builds an aerial mycelium and was isolated from the head of an ant .
NCBI tax id
- NCBI tax id: 2023875
- Matching level: species
strain history
- @ref: 64978
- history: <- C. Liu, School of Life Science, Northeast Agricultural University; 1H-SSA8
doi: 10.13145/bacdive141107.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Streptomyces
- species: Streptomyces amphotericinicus
- full scientific name: Streptomyces amphotericinicus Cao et al. 2017
@ref: 64978
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces amphotericinicus
full scientific name: Streptomyces amphotericinicus Cao et al. 2017
strain designation: 1H-SSA8
type strain: yes
Morphology
cell morphology
- @ref: 44015
- gram stain: positive
multicellular morphology
| @ref | forms multicellular complex | complex name | complex color | medium name |
|---|---|---|---|---|
| 44015 | yes | aerial mycelium | none | International Streptomyces Project medium 1 |
| 44015 | yes | aerial mycelium | yellowish gray | International Streptomyces Project medium 2 |
| 44015 | yes | aerial mycelium | pure white | International Streptomyces Project medium 3 |
| 44015 | yes | aerial mycelium | pure white | International Streptomyces Project medium 4 |
| 44015 | yes | aerial mycelium | deep yellow | International Streptomyces Project medium 5 |
| 44015 | yes | aerial mycelium | none | International Streptomyces Project medium 6 |
| 44015 | yes | aerial mycelium | deep yellowish brown | International Streptomyces Project medium 7 |
| 44015 | yes | aerial mycelium | strong yellowish brown | Czapek's agar |
| 44015 | yes | aerial mycelium | none | nutrient agar |
| 44015 | yes | aerial mycelium | deep yellow | Bennett's agar |
| 44015 | yes | substrate mycelium | transparent | International Streptomyces Project medium 1 |
| 44015 | yes | substrate mycelium | dark grayish yellow | International Streptomyces Project medium 2 |
| 44015 | yes | substrate mycelium | dark yellow | International Streptomyces Project medium 3 |
| 44015 | yes | substrate mycelium | grayish yellow | International Streptomyces Project medium 4 |
| 44015 | yes | substrate mycelium | dark grayish yellow | International Streptomyces Project medium 5 |
| 44015 | yes | substrate mycelium | transparent | International Streptomyces Project medium 6 |
| 44015 | yes | substrate mycelium | grayish brown | International Streptomyces Project medium 7 |
| 44015 | yes | substrate mycelium | moderate brown | Czapek's agar |
| 44015 | yes | substrate mycelium | transparent | nutrient agar |
| 44015 | yes | substrate mycelium | dark yellow | Bennett's agar |
multimedia
- @ref: 64978
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_103128.jpg
- caption: Medium 84 28°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 44015 | oatmeal agar | yes | ||
| 64978 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
| 64978 | ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) | yes | https://mediadive.dsmz.de/medium/84 | Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 44015 | positive | growth | 14-39 | |
| 44015 | positive | optimum | 28 | mesophilic |
| 64978 | positive | growth | 28 | mesophilic |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 44015 | positive | growth | 4.0-9.0 | alkaliphile |
| 44015 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
- @ref: 44015
- oxygen tolerance: aerobe
spore formation
- @ref: 44015
- spore description: spore chains appear as spiral and spores (0.5–1.3x0.4–0.8 µm) are smooth, cylindrical and non-motile
- type of spore: spore
- spore formation: yes
halophily
- @ref: 44015
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0-6 %
observation
- @ref: 44015
- observation: menaquinones are MK-9(H6) , MK-9(H4) and MK-9(H8)
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 44015 | 53424 | tween 20 | - | hydrolysis |
| 44015 | 17632 | nitrate | - | reduction |
| 44015 | 62968 | cellulose | + | assimilation |
| 44015 | 5291 | gelatin | + | assimilation |
| 44015 | milk | + | assimilation | |
| 44015 | esculin ferric citrate | + | hydrolysis | |
| 44015 | 28017 | starch | + | hydrolysis |
| 44015 | 53426 | tween 80 | + | hydrolysis |
metabolite production
| @ref | Chebi-ID | metabolite | production |
|---|---|---|---|
| 44015 | 15138 | sulfide | no |
| 44015 | 2652 | amphotericin b | yes |
enzymes
- @ref: 44015
- value: urease
- activity: +
- ec: 3.5.1.5
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | latitude | longitude |
|---|---|---|---|---|---|---|---|
| 44015 | the head of an ant (Camponotus japonicus Mayr) | Harbin, Heilongjiang | China | CHN | Asia | ||
| 64978 | head of an ant (Camponotus japonicus Mayr) | Heilongjiang Province, Harbin, Northeast Agriculture University (45° 45' N, 126° 41' E) | China | CHN | Asia | 45.75 | 126.683 |
isolation source categories
- Cat1: #Host
- Cat2: #Arthropoda
- Cat3: #Insecta
Safety information
risk assessment
- @ref: 64978
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 64978
- description: Streptomyces amphotericinicus strain 1H-SSA8 16S ribosomal RNA gene, partial sequence
- accession: KX777593
- length: 1519
- database: ena
- NCBI tax ID: 2023875
GC content
- @ref: 64978
- GC-content: 68.8
- method: thermal denaturation, midpoint method (Tm)
External links
@ref: 64978
culture collection no.: CGMCC 4.7350, DSM 103128
straininfo link
- @ref: 96857
- straininfo: 402748
literature
- topic: Phylogeny
- Pubmed-ID: 29034849
- title: Streptomyces amphotericinicus sp. nov., an amphotericin-producing actinomycete isolated from the head of an ant (Camponotus japonicus Mayr).
- authors: Cao T, Mu S, Lu C, Zhao S, Li D, Yan K, Xiang W, Liu C
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.002382
- year: 2017
- mesh: Amphotericin B/*biosynthesis, Animals, Ants/*microbiology, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry
- topic2: Transcriptome
Reference
| @id | authors | title | doi/url | journal | pubmed | catalogue |
|---|---|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
| 44015 | Tingting Cao, Shan Mu, Chang Lu, Shanshan Zhao, Dongmei Li, Kai Yan, Wensheng Xiang, Chongxi Liu | Streptomyces amphotericinicus sp. nov., an amphotericin-producing actinomycete isolated from the head of an ant (Camponotus japonicus Mayr) | 10.1099/ijsem.0.002382 | IJSEM 67: 4967-4973 2017 | 29034849 | |
| 64978 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-103128 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 103128) | |||
| 96857 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID402748.1 |