Strain identifier
BacDive ID: 141091
Type strain:
Species: Psychroflexus planctonicus
Strain Designation: X15M-8
Strain history: Z.-P. Liu; Inst. of Microbiol., CAS, China; X15M-8.
NCBI tax ID(s): 1526575 (species)
General
@ref: 43998
BacDive-ID: 141091
keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-negative, rod-shaped
description: Psychroflexus planctonicus X15M-8 is an aerobe, Gram-negative, rod-shaped bacterium that was isolated from water sample.
NCBI tax id
- NCBI tax id: 1526575
- Matching level: species
strain history
- @ref: 67770
- history: Z.-P. Liu; Inst. of Microbiol., CAS, China; X15M-8.
doi: 10.13145/bacdive141091.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacteroidota
- domain: Bacteria
- phylum: Bacteroidota
- class: Flavobacteriia
- order: Flavobacteriales
- family: Flavobacteriaceae
- genus: Psychroflexus
- species: Psychroflexus planctonicus
- full scientific name: Psychroflexus planctonicus Zhong et al. 2016
@ref: 43998
domain: Bacteria
phylum: Bacteroidetes
class: Flavobacteriia
order: Flavobacteriales
family: Flavobacteriaceae
genus: Psychroflexus
species: Psychroflexus planctonicus
strain designation: X15M-8
type strain: yes
Morphology
cell morphology
- @ref: 43998
- gram stain: negative
- cell length: 1.2-2.2 µm
- cell width: 0.3-0.6 µm
- cell shape: rod-shaped
- motility: no
colony morphology
- @ref: 43998
- colony size: 0.5-2.0 mm
- colony color: orange-red
- incubation period: 4 days
- medium used: marine agar 2216
Culture and growth conditions
culture medium
- @ref: 43998
- name: Marine agar (MA)
- growth: yes
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
43998 | positive | growth | 4-35 | |
43998 | positive | optimum | 25 | mesophilic |
67770 | positive | growth | 25 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
43998 | positive | growth | 6.5-10.0 | alkaliphile |
43998 | positive | optimum | 7.0-7.5 |
Physiology and metabolism
oxygen tolerance
- @ref: 43998
- oxygen tolerance: aerobe
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
43998 | NaCl | positive | growth | 0.5-14.0 % |
43998 | NaCl | positive | optimum | 3 % |
observation
@ref | observation |
---|---|
43998 | glistening after cultivation on MA |
43998 | positive for carotenoid pigments |
43998 | major respiratory quinone is MK-6 |
67770 | quinones: MK-6 |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
43998 | 27613 | amygdalin | - | carbon source |
43998 | 17057 | cellobiose | - | carbon source |
43998 | 15963 | ribitol | - | carbon source |
43998 | 17108 | D-arabinose | - | carbon source |
43998 | 18333 | D-arabitol | - | carbon source |
43998 | 15824 | D-fructose | - | carbon source |
43998 | 28847 | D-fucose | - | carbon source |
43998 | 62318 | D-lyxose | - | carbon source |
43998 | 16899 | D-mannitol | - | carbon source |
43998 | 16024 | D-mannose | - | carbon source |
43998 | 16988 | D-ribose | - | carbon source |
43998 | 17924 | D-sorbitol | - | carbon source |
43998 | 16443 | D-tagatose | - | carbon source |
43998 | 65327 | D-xylose | - | carbon source |
43998 | 16813 | galactitol | - | carbon source |
43998 | 17113 | erythritol | - | carbon source |
43998 | 28066 | gentiobiose | - | carbon source |
43998 | 17754 | glycerol | - | carbon source |
43998 | 28087 | glycogen | - | carbon source |
43998 | 17268 | myo-inositol | - | carbon source |
43998 | 15443 | inulin | - | carbon source |
43998 | 30849 | L-arabinose | - | carbon source |
43998 | 18403 | L-arabitol | - | carbon source |
43998 | 18287 | L-fucose | - | carbon source |
43998 | 62345 | L-rhamnose | - | carbon source |
43998 | 17266 | L-sorbose | - | carbon source |
43998 | 65328 | L-xylose | - | carbon source |
43998 | 17716 | lactose | - | carbon source |
43998 | 6731 | melezitose | - | carbon source |
43998 | 28053 | melibiose | - | carbon source |
43998 | 320061 | methyl alpha-D-glucopyranoside | - | carbon source |
43998 | 43943 | methyl alpha-D-mannoside | - | carbon source |
43998 | 74863 | methyl beta-D-xylopyranoside | - | carbon source |
43998 | 506227 | N-acetylglucosamine | - | carbon source |
43998 | potassium 2-dehydro-D-gluconate | - | carbon source | |
43998 | potassium 5-dehydro-D-gluconate | - | carbon source | |
43998 | 32032 | potassium gluconate | - | carbon source |
43998 | 16634 | raffinose | - | carbon source |
43998 | 27082 | trehalose | - | carbon source |
43998 | 32528 | turanose | - | carbon source |
43998 | 17151 | xylitol | - | carbon source |
43998 | casein | - | hydrolysis | |
43998 | 16467 | L-arginine | - | hydrolysis |
43998 | 17895 | L-tyrosine | - | hydrolysis |
43998 | 53423 | tween 40 | - | hydrolysis |
43998 | 53425 | tween 60 | - | hydrolysis |
43998 | 53426 | tween 80 | - | hydrolysis |
43998 | 16199 | urea | - | hydrolysis |
43998 | 17632 | nitrate | - | reduction |
43998 | 12936 | D-galactose | + | carbon source |
43998 | 18305 | arbutin | + | carbon source |
43998 | 17634 | D-glucose | + | carbon source |
43998 | esculin ferric citrate | + | carbon source | |
43998 | 17306 | maltose | + | carbon source |
43998 | 28017 | starch | + | carbon source |
43998 | 17992 | sucrose | + | carbon source |
43998 | 17634 | D-glucose | + | fermentation |
43998 | 4853 | esculin | + | hydrolysis |
43998 | 5291 | gelatin | + | hydrolysis |
43998 | 28017 | starch | + | hydrolysis |
43998 | 53424 | tween 20 | + | hydrolysis |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
43998 | 35581 | indole | no |
43998 | 16136 | hydrogen sulfide | yes |
enzymes
@ref | value | activity | ec |
---|---|---|---|
43998 | cytochrome oxidase | + | 1.9.3.1 |
43998 | catalase | + | 1.11.1.6 |
43998 | alkaline phosphatase | + | 3.1.3.1 |
43998 | esterase (C 4) | + | |
43998 | esterase Lipase (C 8) | + | |
43998 | leucine arylamidase | + | 3.4.11.1 |
43998 | valine arylamidase | + | |
43998 | trypsin | + | 3.4.21.4 |
43998 | acid phosphatase | + | 3.1.3.2 |
43998 | naphthol-AS-BI-phosphohydrolase | + | |
43998 | beta-glucosidase | + | 3.2.1.21 |
43998 | lipase (C 14) | - | |
43998 | cystine arylamidase | - | 3.4.11.3 |
43998 | alpha-chymotrypsin | - | 3.4.21.1 |
43998 | alpha-galactosidase | - | 3.2.1.22 |
43998 | beta-galactosidase | - | 3.2.1.23 |
43998 | beta-glucuronidase | - | 3.2.1.31 |
43998 | alpha-glucosidase | - | 3.2.1.20 |
43998 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
43998 | alpha-mannosidase | - | 3.2.1.24 |
43998 | alpha-fucosidase | - | 3.2.1.51 |
fatty acid profile
fatty acids
@ref fatty acid percentage 43998 C15:0 anteiso 17.3 43998 C15:1 anteiso A 1.2 43998 C15:0 2OH 5.9 43998 C15:0 3OH 3.5 43998 C15:1ω6c 6.1 43998 C17:0 2OH 5.5 43998 C17:1ω6c 1.5 43998 10-methyl C18:0 1.2 43998 C14:0 iso 1.1 43998 C15:0 iso 11.9 43998 C15:0 iso 3OH 2.7 43998 C15:1 iso G 6.1 43998 C16:0 iso 3OH 5.2 43998 C16:1 iso H 1.4 43998 C17:0 iso 3OH 11.2 - type of FA analysis: whole cell analysis
- incubation medium: marine agar 2216/marine broth 2216
- incubation temperature: 25
- incubation time: 3
- software version: Sherlock 6.0
- library/peak naming table: TSBA6
- system: MIS MIDI
- cutoff value:
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
43998 | water sample | Lake Xiaochaidan in Qaidam basin, Qinghai Province | China | CHN | Asia |
67770 | Lake Xiaochaidan, a salt lake in Qaidam basin | Qinghai Province | China | CHN | Asia |
isolation source categories
- Cat1: #Environmental
- Cat2: #Aquatic
taxonmaps
- @ref: 69479
- File name: preview.99_127320.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_423;96_42397;97_64034;98_85393;99_127320&stattab=map
- Last taxonomy: Psychroflexus planctonicus subclade
- 16S sequence: KJ782429
- Sequence Identity:
- Total samples: 152
- soil counts: 27
- aquatic counts: 120
- animal counts: 5
Sequence information
16S sequences
- @ref: 43998
- description: 16S rRNA gene sequence
- accession: KJ782429
- database: nuccore
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Psychroflexus planctonicus CGMCC 1.12931 | GCA_014640195 | scaffold | ncbi | 1526575 |
66792 | Psychroflexus planctonicus strain CGMCC 1.12931 | 1526575.3 | wgs | patric | 1526575 |
GC content
@ref | GC-content | method |
---|---|---|
43998 | 35 | |
67770 | 35 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
flagellated | no | 94.533 | yes |
gram-positive | no | 98.013 | yes |
anaerobic | no | 98.282 | yes |
aerobic | yes | 85.364 | yes |
halophile | yes | 59.77 | no |
spore-forming | no | 94.788 | no |
glucose-util | yes | 87.012 | no |
thermophile | no | 97.913 | yes |
motile | no | 90.445 | yes |
glucose-ferment | no | 84.443 | yes |
External links
@ref: 43998
culture collection no.: CGMCC 1.12931, JCM 30616
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 26475261 | Psychroflexus salis sp. nov. and Psychroflexus planctonicus sp. nov., isolated from a salt lake. | Zhong ZP, Liu Y, Wang F, Zhou YG, Liu HC, Liu ZP | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000687 | 2015 | Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacteriaceae/*classification/genetics/isolation & purification, Lakes/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, *Saline Waters, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry | Genetics |
Phylogeny | 32453683 | Mesohalobacter halotolerans gen. nov., sp. nov., isolated from a marine solar saltern. | Feng X, Zhang JY, Sang J, Mu DS, Du ZJ | Int J Syst Evol Microbiol | 10.1099/ijsem.0.004143 | 2020 | Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacteriaceae/*classification/isolation & purification, Nucleic Acid Hybridization, Phosphatidylethanolamines/chemistry, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, *Salinity, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry, *Water Microbiology | Transcriptome |
Reference
@id | authors | title | doi/url | journal | pubmed |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
43998 | Zhi-Ping Zhong, Ying Liu, Fang Wang, Yu-Guang Zhou, Hong-Can Liu, Zhi-Pei Liu | Psychroflexus salis sp. nov. and Psychroflexus planctonicus sp. nov., isolated from a salt lake | 10.1099/ijsem.0.000687 | IJSEM 66: 125-131 2016 | 26475261 |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ |