Strain identifier
version 8.1 (current version)
General
@ref: 43970
BacDive-ID: 141077
keywords: 16S sequence, aerobe, Gram-positive, rod-shaped, colony-forming
description: Rhodococcus humicola KACC 18500 is an aerobe, Gram-positive, rod-shaped prokaryote that forms irregular colonies and was isolated from forest topsoil on Kyonggi University campus in Suwon, Gyeonggi-Do, South Korea.
NCBI tax id
- NCBI tax id: 1769756
- Matching level: species
doi: 10.13145/bacdive141077.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- genus: Rhodococcus
- species: Rhodococcus humicola
- full scientific name: Rhodococcus humicola Nguyen and Kim 2016
@ref: 43970
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Mycobacteriales
family: Nocardiaceae
genus: Rhodococcus
species: Rhodococcus humicola
type strain: yes
Morphology
cell morphology
- @ref: 43970
- gram stain: positive
- cell length: 0.8-1.3 µm
- cell width: 0.5-0.8 µm
- cell shape: rod-shaped
- motility: no
colony morphology
- @ref: 43970
- colony color: yellowish
- colony shape: irregular
- medium used: Tryptone soy agar
pigmentation
- @ref: 43970
- production: no
- name: Melanin
Culture and growth conditions
culture medium
| @ref | name | growth |
|---|---|---|
| 43970 | LB (Luria-Bertani) MEDIUM | yes |
| 43970 | MacConkey agar | no |
| 43970 | Nutrient agar (NA) | yes |
| 43970 | Reasoner's 2A agar (R2A) | yes |
| 43970 | Tryptone soy agar | yes |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 43970 | positive | growth | 10-40 | |
| 43970 | positive | optimum | 28-30 | mesophilic |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 43970 | positive | growth | 5.0-10.0 | alkaliphile |
| 43970 | positive | optimum | 6.0-8.0 |
Physiology and metabolism
oxygen tolerance
- @ref: 43970
- oxygen tolerance: aerobe
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 43970 | NaCl | positive | growth | 0-3 %(w/v) |
| 43970 | NaCl | positive | optimum | 0-1 %(w/v) |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 43970 | 15824 | D-fructose | + | builds acid from |
| 43970 | 17992 | sucrose | + | builds acid from |
| 43970 | 4853 | esculin | + | degradation |
| 43970 | 28017 | starch | + | degradation |
| 43970 | 53423 | tween 40 | + | degradation |
| 43970 | 53425 | tween 60 | + | degradation |
| 43970 | 17266 | L-sorbose | +/- | builds acid from |
| 43970 | 28017 | starch | +/- | builds acid from |
| 43970 | 16136 | hydrogen sulfide | +/- | degradation |
| 43970 | 53426 | tween 80 | +/- | degradation |
metabolite production
- @ref: 43970
- Chebi-ID: 16136
- metabolite: hydrogen sulfide
- production: no
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 43970 | cytochrome oxidase | - | 1.9.3.1 |
| 43970 | urease | - | 3.5.1.5 |
| 43970 | DNase | - | |
| 43970 | catalase | + | 1.11.1.6 |
| 43970 | alkaline phosphatase | + | 3.1.3.1 |
| 43970 | esterase (C 4) | + | |
| 43970 | valine arylamidase | + | |
| 43970 | leucine arylamidase | + | 3.4.11.1 |
| 43970 | acid phosphatase | + | 3.1.3.2 |
| 43970 | naphthol-AS-BI-phosphohydrolase | + | |
| 43970 | alpha-glucosidase | + | 3.2.1.20 |
| 43970 | beta-glucosidase | + | 3.2.1.21 |
| 43970 | esterase Lipase (C 8) | +/- | |
| 43970 | lipase (C 14) | +/- | |
| 43970 | cystine arylamidase | - | 3.4.11.3 |
| 43970 | alpha-chymotrypsin | - | 3.4.21.1 |
| 43970 | trypsin | - | 3.4.21.4 |
| 43970 | alpha-galactosidase | - | 3.2.1.22 |
| 43970 | beta-galactosidase | - | 3.2.1.23 |
| 43970 | beta-glucuronidase | - | 3.2.1.31 |
| 43970 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
| 43970 | alpha-mannosidase | - | 3.2.1.24 |
| 43970 | alpha-fucosidase | - | 3.2.1.51 |
Isolation, sampling and environmental information
isolation
- @ref: 43970
- sample type: forest topsoil on Kyonggi University campus in Suwon, Gyeonggi-Do, South Korea
- sampling date: 2015-06
- geographic location: Suwon, Gyeonggi-Do
- country: Republic of Korea
- origin.country: KOR
- continent: Asia
- latitude: 37.3164
- longitude: 127.019
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Terrestrial | #Forest |
| #Environmental | #Terrestrial | #Soil |
Sequence information
16S sequences
- @ref: 43970
- description: 16S rRNA gene sequence
- accession: KT301939
- database: nuccore
GC content
- @ref: 43970
- GC-content: 68.8
- method: high performance liquid chromatography (HPLC)
External links
@ref: 43970
culture collection no.: KACC 18500, NBRC 111581, UC 33
literature
- topic: Phylogeny
- Pubmed-ID: 27031370
- title: Rhodococcus pedocola sp. nov. and Rhodococcus humicola sp. nov., two antibiotic-producing actinomycetes isolated from soil.
- authors: Nguyen TM, Kim J
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.001039
- year: 2016
- mesh: Anti-Bacterial Agents/*biosynthesis, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Forests, Mycolic Acids/chemistry, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Rhodococcus/*classification/genetics/isolation & purification, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry
- topic2: Transcriptome
Reference
| @id | authors | title | doi/url | journal | pubmed |
|---|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
| 43970 | Tuan Manh Nguyen, Jaisoo Kim | Rhodococcus pedocola sp. nov. and Rhodococcus humicola sp. nov., two antibiotic-producing actinomycetes isolated from soil | 10.1099/ijsem.0.001039 | IJSEM 66: 2362-2369 2016 | 27031370 |