Strain identifier

BacDive ID: 141074

Type strain: Yes

Species: Agromyces insulae

Strain history: CCTCC AB 2014301 <-- G.-X. Nie; Henan Normal Univ., China; CFH S0483.

NCBI tax ID(s): 1644105 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 9 (current version):
version 8.1:
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 9 (current version)

General

@ref: 43966

BacDive-ID: 141074

keywords: 16S sequence, Bacteria, aerobe, Gram-positive, rod-shaped, colony-forming

description: Agromyces insulae KCTC 39117 is an aerobe, Gram-positive, rod-shaped bacterium that forms circular colonies and was isolated from soil sample collected from Catba island in Halong Bay, Vietnam.

NCBI tax id

  • NCBI tax id: 1644105
  • Matching level: species

strain history

  • @ref: 67770
  • history: CCTCC AB 2014301 <-- G.-X. Nie; Henan Normal Univ., China; CFH S0483.

doi: 10.13145/bacdive141074.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Microbacteriaceae
  • genus: Agromyces
  • species: Agromyces insulae
  • full scientific name: Agromyces insulae Huang et al. 2016

@ref: 43966

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Micrococcales

family: Microbacteriaceae

genus: Agromyces

species: Agromyces insulae

type strain: yes

Morphology

cell morphology

  • @ref: 43966
  • gram stain: positive
  • cell length: 0.5-2.7 µm
  • cell width: 0.2-0.3 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 43966
  • colony color: cream white
  • colony shape: circular
  • medium used: R2A agar

Culture and growth conditions

culture medium

  • @ref: 43966
  • name: Reasoner's 2A agar (R2A)
  • growth: yes

culture temp

@refgrowthtypetemperature
43966positivegrowth10-37
67770positivegrowth28

culture pH

  • @ref: 43966
  • ability: positive
  • type: growth
  • pH: 7.0-9.0
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 43966
  • oxygen tolerance: aerobe

halophily

  • @ref: 43966
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-2 %(w/v)

observation

@refobservation
43966quinone MK-12
67770quinones: MK-12

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4396627613amygdalin-carbon source
4396616988D-ribose-carbon source
4396617151xylitol-carbon source
4396665327D-xylose-carbon source
4396630849L-arabinose-carbon source
4396662345L-rhamnose-carbon source
4396617716lactose-carbon source
4396628053melibiose-carbon source
4396617268myo-inositol-carbon source
4396616634raffinose-carbon source
4396630031succinate-carbon source
4396653424tween 20-degradation
4396653423tween 40-degradation
4396653425tween 60-degradation
4396653426tween 80-degradation
4396617234glucose-fermentation
4396685146carboxymethylcellulose-hydrolysis
43966casein-hydrolysis
4396617029chitin-hydrolysis
4396628017starch-hydrolysis
4396618186tyrosine-hydrolysis
4396637166xylan-hydrolysis
4396615428glycine-nitrogen source
4396616977L-alanine-nitrogen source
4396617196L-asparagine-nitrogen source
4396615971L-histidine-nitrogen source
4396616857L-threonine-nitrogen source
4396617895L-tyrosine-nitrogen source
4396616414L-valine-nitrogen source
4396617632nitrate-reduction
4396617057cellobiose+carbon source
4396615824D-fructose+carbon source
4396612936D-galactose+carbon source
4396617634D-glucose+carbon source
4396616899D-mannitol+carbon source
4396616024D-mannose+carbon source
4396632528turanose+carbon source
4396623652dextrin+carbon source
439664853esculin+carbon source
4396628066gentiobiose+carbon source
4396617754glycerol+carbon source
4396618287L-fucose+carbon source
4396617306maltose+carbon source
4396617814salicin+carbon source
4396630911sorbitol+carbon source
4396617992sucrose+carbon source
4396627082trehalose+carbon source
439664853esculin+hydrolysis
439665291gelatin+hydrolysis
4396629985L-glutamate+nitrogen source
4396617191L-isoleucine+nitrogen source
4396617203L-proline+nitrogen source

metabolite production

  • @ref: 43966
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
43966catalase-1.11.1.6
43966cytochrome oxidase-1.9.3.1
43966urease-3.5.1.5
43966arginine dihydrolase-3.5.3.6
43966N-acetyl-beta-glucosaminidase+3.2.1.52
43966alkaline phosphatase+3.1.3.1
43966acid phosphatase+3.1.3.2
43966cystine arylamidase+3.4.11.3
43966esterase (C 4)+
43966esterase Lipase (C 8)+
43966beta-galactosidase+3.2.1.23
43966alpha-glucosidase+3.2.1.20
43966beta-glucosidase+3.2.1.21
43966leucine arylamidase+3.4.11.1
43966naphthol-AS-BI-phosphohydrolase+
43966trypsin+3.4.21.4
43966valine arylamidase+
43966alpha-chymotrypsin-3.4.21.1
43966alpha-fucosidase-3.2.1.51
43966alpha-galactosidase-3.2.1.22
43966beta-glucuronidase-3.2.1.31
43966lipase (C 14)-
43966alpha-mannosidase-3.2.1.24

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43966C15:0 anteiso36.8
    43966C17:0 anteiso34.5
    43966C19:0 anteiso0.3
    43966C16:00.5
    43966C14:0 iso1.3
    43966C15:0 iso4.8
    43966C16:0 iso20.3
    43966C17:0 iso1.3
    43966C18:0 iso0.3
  • type of FA analysis: whole cell analysis
  • incubation medium: Tryptic soy agar
  • agar/liquid: agar
  • incubation temperature: 28
  • incubation time: 3
  • software version: Sherlock 6.1
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • method/protocol: Sasser, 1990

Isolation, sampling and environmental information

isolation

@refsample typesampling dategeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment culture
43966soil sample collected from Catba island in Halong Bay, Vietnam2012-03Halong BayVietnamVNMAsia20.7733107.121R2A agar
67770Soil from Catba Island in Halong BayVietnamVNMAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Sequence information

16S sequences

  • @ref: 43966
  • description: Agromyces insulae strain CFH S0483 16S ribosomal RNA gene, partial sequence
  • accession: KP232915
  • database: nuccore

GC content

  • @ref: 43966
  • GC-content: 71.6
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 43966

culture collection no.: KCTC 39117, CCTCC AB 2014301, CFH S0483, JCM 31741, BCRC 16983

literature

  • topic: Phylogeny
  • Pubmed-ID: 26883212
  • title: Agromyces insulae sp. nov., an actinobacterium isolated from a soil sample.
  • authors: Huang JR, Ming H, Li S, Meng XL, Zhang JX, Khieu TN, Tang Z, Li WJ, Nie GX
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.000978
  • year: 2016
  • mesh: Actinomycetales/*classification/genetics/isolation & purification, Aminobutyrates/chemistry, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vietnam, Vitamin K 2/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43966Jian-Rong Huang, Hong Ming, Shuai Li, Xiao-Lin Meng, Jian-Xin Zhang, Thi-Nhan Khieu, Zhong Tang, Wen-Jun Li, Guo-Xing NieAgromyces insulae sp. nov., an actinobacterium isolated from a soil sample10.1099/ijsem.0.000978IJSEM 66: 2002-2007 201626883212
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/