Strain identifier

BacDive ID: 141061

Type strain: Yes

Species: Helicobacter apri

Strain Designation: A 19

Strain history: <- S. Piva, University of Bologna - Department of Veterinary Medical Sciences; A 19

NCBI tax ID(s): 1576610 (species)

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General

@ref: 64817

BacDive-ID: 141061

DSM-Number: 28990

keywords: 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, spiral-shaped, colony-forming

description: Helicobacter apri A 19 is a microaerophile, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from gastric mucosa and caecal contents of three different wild boars .

NCBI tax id

  • NCBI tax id: 1576610
  • Matching level: species

strain history

  • @ref: 64817
  • history: <- S. Piva, University of Bologna - Department of Veterinary Medical Sciences; A 19

doi: 10.13145/bacdive141061.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Epsilonproteobacteria
  • order: Campylobacterales
  • family: Helicobacteraceae
  • genus: Helicobacter
  • species: Helicobacter apri
  • full scientific name: Helicobacter apri Zanoni et al. 2016

@ref: 64817

domain: Bacteria

phylum: Proteobacteria

class: Epsilonproteobacteria

order: Campylobacterales

family: Helicobacteraceae

genus: Helicobacter

species: Helicobacter apri

full scientific name: Helicobacter apri Zanoni et al. 2016

strain designation: A 19

type strain: yes

Morphology

cell morphology

  • @ref: 43940
  • gram stain: negative
  • cell length: 2 µm
  • cell width: 0.4 µm
  • cell shape: spiral-shaped

colony morphology

  • @ref: 43940
  • type of hemolysis: gamma
  • colony size: 7-8 mm
  • colony color: yellow-grey
  • colony shape: circular
  • incubation period: 2 days
  • medium used: Nutrient sheep blood agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43940Nutrient sheep blood agaryes
64817COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base

culture temp

@refgrowthtypetemperaturerange
43940positivegrowth37mesophilic
43940positivegrowth42thermophilic
64817positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 43940
  • oxygen tolerance: microaerophile

spore formation

  • @ref: 43940
  • spore formation: no

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
43940606565hippurate-hydrolysis
4394017632nitrate+reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
43940100147nalidixic acidyesyes30 µg (disc)
43940124991cefalotinyesyes30 µg (disc)

enzymes

@refvalueactivityec
43940cytochrome oxidase+1.9.3.1
43940catalase1.11.1.6
43940alkaline phosphatase3.1.3.1
43940urease-3.5.1.5
43940gamma-glutamyltransferase2.3.2.2

Isolation, sampling and environmental information

isolation

@refsample typesampling dateenrichment cultureenrichment culture durationenrichment culture temperaturegeographic locationcountryorigin.countrycontinentlatitudelongitude
43940gastric mucosa and caecal contents of three different wild boars (Sus scrofa)2011Nutrient sheep-blood agar4 days37
64817caecal contents of a wild boar (Sus scrofa)Bologna Province, Apennine MountainsItalyITAEurope4214

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Suidae (Pig,Swine)
#Host Body Product#Gastrointestinal tract#Caecal content
#Host Body-Site#Gastrointestinal tract

Sequence information

16S sequences

  • @ref: 64817
  • description: Helicobacter apri strain A19 16S ribosomal RNA gene, partial sequence
  • accession: KP120975
  • length: 1099
  • database: ena
  • NCBI tax ID: 1576610

GC content

  • @ref: 64817
  • GC-content: 40.2
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 64817

culture collection no.: DSM 28990, LMG 28471

straininfo link

  • @ref: 96850
  • straininfo: 407918

literature

  • topic: Phylogeny
  • Pubmed-ID: 27064324
  • title: Helicobacter apri sp. nov., isolated from wild boars.
  • authors: Zanoni RG, Piva S, Florio D, Bassi P, Mion D, Cnockaert M, Luchetti A, Vandamme P
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001071
  • year: 2016
  • mesh: Animals, Bacterial Typing Techniques, Base Composition, Chaperonin 60/genetics, DNA, Bacterial/genetics, Gastric Mucosa/*microbiology, Genes, Bacterial, Helicobacter/*classification/genetics/isolation & purification, Italy, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sus scrofa/*microbiology
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43940Renato Giulio Zanoni, Silvia Piva, Daniela Florio, Patrizia Bassi, Domenico Mion, Margo Cnockaert, Andrea Luchetti, Peter VandammeHelicobacter apri sp. nov., isolated from wild boars10.1099/ijsem.0.001071IJSEM 66: 2876-2882 201627064324
64817Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-28990Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 28990)
96850Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID407918.1