Strain identifier
BacDive ID: 141005
Type strain:
Species: Prauserella oleivorans
Strain Designation: RIPI
NCBI tax ID(s): 1478153 (species)
version 8.1 (current version)
General
@ref: 43896
BacDive-ID: 141005
keywords: 16S sequence, Bacteria, aerobe, spore-forming
description: Prauserella oleivorans RIPI is an aerobe, spore-forming bacterium that was isolated from watery sludge sample from oil-contaminated mud pit in Khangiran oil and gas field, north-east Iran.
NCBI tax id
- NCBI tax id: 1478153
- Matching level: species
doi: 10.13145/bacdive141005.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Pseudonocardiales
- family: Pseudonocardiaceae
- genus: Prauserella
- species: Prauserella oleivorans
- full scientific name: Prauserella oleivorans Dastgheib et al. 2017
@ref: 43896
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Pseudonocardiales
family: Pseudonocardiaceae
genus: Prauserella
species: Prauserella oleivorans
strain designation: RIPI
type strain: yes
Morphology
colony morphology
@ref | colony color | medium used |
---|---|---|
43896 | light yellow | ISP 2 |
43896 | light yellow | ISP 3 agar |
43896 | light yellow | ISP 4 |
Culture and growth conditions
culture medium
@ref | name | growth | composition |
---|---|---|---|
43896 | Bushnell-Haas mineral medium | yes | supplemeted with 5% (w/v) NaCl, 0.1% (v/v) crude oil |
43896 | ISP 2 agar | yes | supplemeted with 5% (w/v) NaCl |
43896 | ISP 3 agar | yes | supplemeted with 5% (w/v) NaCl |
43896 | ISP 4 agar | yes | supplemeted with 5% (w/v) NaCl |
43896 | ISP 5 agar | yes | supplemeted with 5% (w/v) NaCl |
43896 | Reasoner's 2A agar (R2A) | yes | supplemeted with 5% (w/v) NaCl |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
43896 | positive | growth | 25-55 | |
43896 | positive | optimum | 45 | thermophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
43896 | positive | growth | 6.0-9.5 | alkaliphile |
43896 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
- @ref: 43896
- oxygen tolerance: aerobe
spore formation
- @ref: 43896
- spore description: non-motile spores
- type of spore: spore
- spore formation: yes
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
43896 | NaCl | positive | growth | 0-12.5 %(w/v) |
43896 | NaCl | positive | optimum | 3-5 %(w/v) |
observation
- @ref: 43896
- observation: quinone MK-9
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
43896 | 18240 | 4-hydroxy-L-proline | - | carbon source |
43896 | 17924 | D-sorbitol | - | carbon source |
43896 | 16467 | L-arginine | - | carbon source |
43896 | 29991 | L-aspartate | - | carbon source |
43896 | 29985 | L-glutamate | - | carbon source |
43896 | 17191 | L-isoleucine | - | carbon source |
43896 | 15603 | L-leucine | - | carbon source |
43896 | 17203 | L-proline | - | carbon source |
43896 | 62345 | L-rhamnose | - | carbon source |
43896 | 16857 | L-threonine | - | carbon source |
43896 | 29864 | mannitol | - | carbon source |
43896 | 16634 | raffinose | - | carbon source |
43896 | 17151 | xylitol | - | carbon source |
43896 | 16708 | adenine | - | hydrolysis |
43896 | 18305 | arbutin | - | hydrolysis |
43896 | 4853 | esculin | - | hydrolysis |
43896 | 17368 | hypoxanthine | - | hydrolysis |
43896 | 16301 | nitrite | - | hydrolysis |
43896 | 28017 | starch | - | hydrolysis |
43896 | 16199 | urea | - | hydrolysis |
43896 | 15318 | xanthine | - | hydrolysis |
43896 | 37166 | xylan | - | hydrolysis |
43896 | 15963 | ribitol | + | carbon source |
43896 | 17057 | cellobiose | + | carbon source |
43896 | 17634 | D-glucose | + | carbon source |
43896 | 16024 | D-mannose | + | carbon source |
43896 | 16988 | D-ribose | + | carbon source |
43896 | 65327 | D-xylose | + | carbon source |
43896 | 15428 | glycine | + | carbon source |
43896 | 17268 | myo-inositol | + | carbon source |
43896 | L-alanine 4-nitroanilide | + | carbon source | |
43896 | 30849 | L-arabinose | + | carbon source |
43896 | 17196 | L-asparagine | + | carbon source |
43896 | 17561 | L-cysteine | + | carbon source |
43896 | 18050 | L-glutamine | + | carbon source |
43896 | 15971 | L-histidine | + | carbon source |
43896 | 18019 | L-lysine | + | carbon source |
43896 | 16643 | L-methionine | + | carbon source |
43896 | 15729 | L-ornithine | + | carbon source |
43896 | 17115 | L-serine | + | carbon source |
43896 | 17895 | L-tyrosine | + | carbon source |
43896 | 17716 | lactose | + | carbon source |
43896 | 17306 | maltose | + | carbon source |
43896 | 28053 | melibiose | + | carbon source |
43896 | 17814 | salicin | + | carbon source |
43896 | 27082 | trehalose | + | carbon source |
43896 | casein | + | hydrolysis | |
43896 | 16991 | dna | + | hydrolysis |
43896 | 5291 | gelatin | + | hydrolysis |
43896 | 17632 | nitrate | + | hydrolysis |
43896 | 53424 | tween 20 | + | hydrolysis |
43896 | 53423 | tween 40 | + | hydrolysis |
43896 | 53425 | tween 60 | + | hydrolysis |
43896 | 53426 | tween 80 | + | hydrolysis |
43896 | 18186 | tyrosine | + | hydrolysis |
metabolite production
- @ref: 43896
- Chebi-ID: 16136
- metabolite: hydrogen sulfide
- production: no
Isolation, sampling and environmental information
isolation
- @ref: 43896
- sample type: watery sludge sample from oil-contaminated mud pit in Khangiran oil and gas field, north-east Iran
- country: Iran
- origin.country: IRN
- continent: Asia
- latitude: 36.5556
- longitude: 60.7992
- enrichment culture: R2A agar
- enrichment culture composition: supplemented with 5% (w/v) NaCl
- enrichment culture duration: 14 days
- enrichment culture temperature: 30
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Contamination | #Oil (Fuel) |
#Environmental | #Terrestrial | #Mud (Sludge) |
taxonmaps
- @ref: 69479
- File name: preview.99_95316.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_7302;97_8756;98_65409;99_95316&stattab=map
- Last taxonomy: Prauserella oleivorans subclade
- 16S sequence: KJ123726
- Sequence Identity:
- Total samples: 83
- soil counts: 38
- aquatic counts: 11
- animal counts: 32
- plant counts: 2
Sequence information
16S sequences
- @ref: 43896
- description: 16S rRNA gene sequence
- accession: KJ123726
- database: nuccore
GC content
- @ref: 43896
- GC-content: 69
- method: high performance liquid chromatography (HPLC)
External links
@ref: 43896
culture collection no.: IBRC-M 10906, LMG 28389
literature
- topic: Phylogeny
- Pubmed-ID: 28857021
- title: Prauserella oleivorans sp. nov., a halophilic and thermotolerant crude-oil-degrading actinobacterium isolated from an oil-contaminated mud pit.
- authors: Dastgheib SMM, Tirandaz H, Moshtaghi Nikou M, Ramezani M, Shavandi M, Amoozegar MA, Ventosa A
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.002124
- year: 2017
- mesh: Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Iran, Nucleic Acid Hybridization, Oil and Gas Fields/*microbiology, Peptidoglycan/chemistry, Petroleum/*microbiology, Petroleum Pollution, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Soil Pollutants, Vitamin K 2/analogs & derivatives/chemistry
- topic2: Transcriptome
Reference
@id | authors | title | doi/url | journal | pubmed |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
43896 | Seyed Mohammad Mehdi Dastgheib, Hassan Tirandaz, Mahdi Moshtaghi Nikou, Mohaddaseh Ramezani, Mahmoud Shavandi, Mohammad Ali Amoozegar, Antonio Ventosa | Prauserella oleivorans sp. nov., a halophilic and thermotolerant crude-oil-degrading actinobacterium isolated from an oil-contaminated mud pit | 10.1099/ijsem.0.002124 | IJSEM 67: 3381-3386 2017 | 28857021 |
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ |