Strain identifier

BacDive ID: 141005

Type strain: Yes

Species: Prauserella oleivorans

Strain Designation: RIPI

NCBI tax ID(s): 1478153 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 43896

BacDive-ID: 141005

keywords: 16S sequence, Bacteria, aerobe, spore-forming

description: Prauserella oleivorans RIPI is an aerobe, spore-forming bacterium that was isolated from watery sludge sample from oil-contaminated mud pit in Khangiran oil and gas field, north-east Iran.

NCBI tax id

  • NCBI tax id: 1478153
  • Matching level: species

doi: 10.13145/bacdive141005.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Prauserella
  • species: Prauserella oleivorans
  • full scientific name: Prauserella oleivorans Dastgheib et al. 2017

@ref: 43896

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Pseudonocardiales

family: Pseudonocardiaceae

genus: Prauserella

species: Prauserella oleivorans

strain designation: RIPI

type strain: yes

Morphology

colony morphology

@refcolony colormedium used
43896light yellowISP 2
43896light yellowISP 3 agar
43896light yellowISP 4

Culture and growth conditions

culture medium

@refnamegrowthcomposition
43896Bushnell-Haas mineral mediumyessupplemeted with 5% (w/v) NaCl, 0.1% (v/v) crude oil
43896ISP 2 agaryessupplemeted with 5% (w/v) NaCl
43896ISP 3 agaryessupplemeted with 5% (w/v) NaCl
43896ISP 4 agaryessupplemeted with 5% (w/v) NaCl
43896ISP 5 agaryessupplemeted with 5% (w/v) NaCl
43896Reasoner's 2A agar (R2A)yessupplemeted with 5% (w/v) NaCl

culture temp

@refgrowthtypetemperaturerange
43896positivegrowth25-55
43896positiveoptimum45thermophilic

culture pH

@refabilitytypepHPH range
43896positivegrowth6.0-9.5alkaliphile
43896positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 43896
  • oxygen tolerance: aerobe

spore formation

  • @ref: 43896
  • spore description: non-motile spores
  • type of spore: spore
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
43896NaClpositivegrowth0-12.5 %(w/v)
43896NaClpositiveoptimum3-5 %(w/v)

observation

  • @ref: 43896
  • observation: quinone MK-9

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
43896182404-hydroxy-L-proline-carbon source
4389617924D-sorbitol-carbon source
4389616467L-arginine-carbon source
4389629991L-aspartate-carbon source
4389629985L-glutamate-carbon source
4389617191L-isoleucine-carbon source
4389615603L-leucine-carbon source
4389617203L-proline-carbon source
4389662345L-rhamnose-carbon source
4389616857L-threonine-carbon source
4389629864mannitol-carbon source
4389616634raffinose-carbon source
4389617151xylitol-carbon source
4389616708adenine-hydrolysis
4389618305arbutin-hydrolysis
438964853esculin-hydrolysis
4389617368hypoxanthine-hydrolysis
4389616301nitrite-hydrolysis
4389628017starch-hydrolysis
4389616199urea-hydrolysis
4389615318xanthine-hydrolysis
4389637166xylan-hydrolysis
4389615963ribitol+carbon source
4389617057cellobiose+carbon source
4389617634D-glucose+carbon source
4389616024D-mannose+carbon source
4389616988D-ribose+carbon source
4389665327D-xylose+carbon source
4389615428glycine+carbon source
4389617268myo-inositol+carbon source
43896L-alanine 4-nitroanilide+carbon source
4389630849L-arabinose+carbon source
4389617196L-asparagine+carbon source
4389617561L-cysteine+carbon source
4389618050L-glutamine+carbon source
4389615971L-histidine+carbon source
4389618019L-lysine+carbon source
4389616643L-methionine+carbon source
4389615729L-ornithine+carbon source
4389617115L-serine+carbon source
4389617895L-tyrosine+carbon source
4389617716lactose+carbon source
4389617306maltose+carbon source
4389628053melibiose+carbon source
4389617814salicin+carbon source
4389627082trehalose+carbon source
43896casein+hydrolysis
4389616991dna+hydrolysis
438965291gelatin+hydrolysis
4389617632nitrate+hydrolysis
4389653424tween 20+hydrolysis
4389653423tween 40+hydrolysis
4389653425tween 60+hydrolysis
4389653426tween 80+hydrolysis
4389618186tyrosine+hydrolysis

metabolite production

  • @ref: 43896
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: no

Isolation, sampling and environmental information

isolation

  • @ref: 43896
  • sample type: watery sludge sample from oil-contaminated mud pit in Khangiran oil and gas field, north-east Iran
  • country: Iran
  • origin.country: IRN
  • continent: Asia
  • latitude: 36.5556
  • longitude: 60.7992
  • enrichment culture: R2A agar
  • enrichment culture composition: supplemented with 5% (w/v) NaCl
  • enrichment culture duration: 14 days
  • enrichment culture temperature: 30

isolation source categories

Cat1Cat2Cat3
#Engineered#Contamination#Oil (Fuel)
#Environmental#Terrestrial#Mud (Sludge)

taxonmaps

  • @ref: 69479
  • File name: preview.99_95316.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_7302;97_8756;98_65409;99_95316&stattab=map
  • Last taxonomy: Prauserella oleivorans subclade
  • 16S sequence: KJ123726
  • Sequence Identity:
  • Total samples: 83
  • soil counts: 38
  • aquatic counts: 11
  • animal counts: 32
  • plant counts: 2

Sequence information

16S sequences

  • @ref: 43896
  • description: 16S rRNA gene sequence
  • accession: KJ123726
  • database: nuccore

GC content

  • @ref: 43896
  • GC-content: 69
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 43896

culture collection no.: IBRC-M 10906, LMG 28389

literature

  • topic: Phylogeny
  • Pubmed-ID: 28857021
  • title: Prauserella oleivorans sp. nov., a halophilic and thermotolerant crude-oil-degrading actinobacterium isolated from an oil-contaminated mud pit.
  • authors: Dastgheib SMM, Tirandaz H, Moshtaghi Nikou M, Ramezani M, Shavandi M, Amoozegar MA, Ventosa A
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002124
  • year: 2017
  • mesh: Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Iran, Nucleic Acid Hybridization, Oil and Gas Fields/*microbiology, Peptidoglycan/chemistry, Petroleum/*microbiology, Petroleum Pollution, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Soil Pollutants, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43896Seyed Mohammad Mehdi Dastgheib, Hassan Tirandaz, Mahdi Moshtaghi Nikou, Mohaddaseh Ramezani, Mahmoud Shavandi, Mohammad Ali Amoozegar, Antonio VentosaPrauserella oleivorans sp. nov., a halophilic and thermotolerant crude-oil-degrading actinobacterium isolated from an oil-contaminated mud pit10.1099/ijsem.0.002124IJSEM 67: 3381-3386 201728857021
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/