Strain identifier
BacDive ID: 140975
Type strain:
Species: Massilia solisilvae
Strain Designation: J18
Strain history: J. Kim; Kyonggi Univ., South Korea; J18.
NCBI tax ID(s): 1811225 (species)
General
@ref: 43867
BacDive-ID: 140975
keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-negative, motile, rod-shaped, colony-forming
description: Massilia solisilvae J18 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from forest soil at Kyonggi University, Suwon, Gyeonggi-Do, South Korea.
NCBI tax id
- NCBI tax id: 1811225
- Matching level: species
strain history
- @ref: 67770
- history: J. Kim; Kyonggi Univ., South Korea; J18.
doi: 10.13145/bacdive140975.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Burkholderiales
- family: Oxalobacteraceae
- genus: Massilia
- species: Massilia solisilvae
- full scientific name: Massilia solisilvae Altankhuu and Kim 2017
synonyms
- @ref: 20215
- synonym: Telluria solisilvae
@ref: 43867
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Burkholderiales
family: Oxalobacteraceae
genus: Massilia
species: Massilia solisilvae
strain designation: J18
type strain: yes
Morphology
cell morphology
- @ref: 43867
- gram stain: negative
- cell length: 2.1-2.3 µm
- cell width: 0.4-0.5 µm
- cell shape: rod-shaped
- motility: yes
- flagellum arrangement: polar
colony morphology
- @ref: 43867
- colony size: 0.5-0.8 mm
- colony color: white
- colony shape: circular
- incubation period: 2 days
- medium used: R2A agar
Culture and growth conditions
culture medium
@ref | name | growth |
---|---|---|
43867 | Brain-Heart infusion agar | no |
43867 | LB (Luria-Bertani) MEDIUM | yes |
43867 | Marine agar (MA) | no |
43867 | Nutrient agar (NA) | yes |
43867 | Reasoner's 2A agar (R2A) | yes |
43867 | Tryptic soy agar | no |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
43867 | positive | growth | 4-45 | |
43867 | positive | optimum | 28-37 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
43867 | positive | growth | 4.0-10.0 | alkaliphile |
43867 | positive | optimum | 6.0-8.0 |
Physiology and metabolism
oxygen tolerance
- @ref: 43867
- oxygen tolerance: aerobe
spore formation
- @ref: 43867
- spore formation: no
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
43867 | NaCl | positive | optimum | 0 %(w/v) |
43867 | NaCl | positive | growth | 0-1 %(w/v) |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
43867 | 37054 | 3-hydroxybutyrate | - | assimilation |
43867 | 17879 | 4-hydroxybenzoate | - | assimilation |
43867 | 355715 | 4-nitrophenyl beta-D-galactopyranoside | - | assimilation |
43867 | 17634 | D-glucose | - | assimilation |
43867 | 16024 | D-mannose | - | assimilation |
43867 | 17203 | L-proline | - | assimilation |
43867 | 17115 | L-serine | - | assimilation |
43867 | 24996 | lactate | - | assimilation |
43867 | 25115 | malate | - | assimilation |
43867 | 18401 | phenylacetate | - | assimilation |
43867 | 32032 | potassium gluconate | - | assimilation |
43867 | 53258 | sodium citrate | - | assimilation |
43867 | 9300 | suberic acid | - | assimilation |
43867 | 17992 | sucrose | - | assimilation |
43867 | 31011 | valerate | - | assimilation |
43867 | 27897 | tryptophan | - | energy source |
43867 | casein | - | hydrolysis | |
43867 | 5291 | gelatin | - | hydrolysis |
43867 | 28017 | starch | - | hydrolysis |
43867 | 16199 | urea | - | hydrolysis |
43867 | 17128 | adipate | + | assimilation |
43867 | 28087 | glycogen | + | assimilation |
43867 | 16991 | dna | + | hydrolysis |
43867 | 4853 | esculin | + | hydrolysis |
43867 | 53423 | tween 40 | + | hydrolysis |
43867 | 53425 | tween 60 | + | hydrolysis |
43867 | 53426 | tween 80 | + | hydrolysis |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
43867 | 16136 | hydrogen sulfide | no |
43867 | 35581 | indole | no |
metabolite tests
- @ref: 43867
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
43867 | catalase | + | 1.11.1.6 |
43867 | cytochrome oxidase | + | 1.9.3.1 |
43867 | tryptophan deaminase | - | 4.1.99.1 |
43867 | cystine arylamidase | + | 3.4.11.3 |
43867 | alpha-glucosidase | + | 3.2.1.20 |
43867 | alpha-chymotrypsin | - | 3.4.21.1 |
43867 | beta-galactosidase | - | 3.2.1.23 |
43867 | beta-glucosidase | - | 3.2.1.21 |
43867 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
43867 | alpha-fucosidase | - | 3.2.1.51 |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | enrichment culture | enrichment culture duration | enrichment culture temperature |
---|---|---|---|---|---|---|---|---|
43867 | forest soil at Kyonggi University, Suwon, Gyeonggi-Do, South Korea | Suwon, Gyeonggi-Do | Republic of Korea | KOR | Asia | Soil extract-R2A agar | 5 weeks | 20 |
67770 | Forest soil at Kyonggi Univ. | Republic of Korea | KOR | Asia |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Terrestrial | #Forest |
#Environmental | #Terrestrial | #Soil |
Sequence information
16S sequences
- @ref: 43867
- description: 16S rRNA gene sequence
- accession: KU870758
- database: nuccore
Genome sequences
- @ref: 66792
- description: Massilia solisilvae JCM 31607
- accession: GCA_024753215
- assembly level: contig
- database: ncbi
- NCBI tax ID: 1811225
GC content
- @ref: 67770
- GC-content: 63.4
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | yes | 87.938 | no |
flagellated | yes | 80.755 | yes |
gram-positive | no | 98.885 | no |
anaerobic | no | 98.703 | yes |
aerobic | yes | 85.466 | yes |
halophile | no | 93.889 | yes |
spore-forming | no | 93.173 | no |
glucose-ferment | no | 89.447 | no |
thermophile | no | 97.525 | yes |
glucose-util | no | 62.918 | yes |
External links
@ref: 43867
culture collection no.: KEMB 9005-366, JCM 31607
literature
- topic: Phylogeny
- Pubmed-ID: 28820094
- title: Massilia solisilvae sp. nov., Massilia terrae sp. nov. and Massilia agilis sp. nov., isolated from forest soil in South Korea by using a newly developed culture method.
- authors: Altankhuu K, Kim J
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.002076
- year: 2017
- mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Forests, Nucleic Acid Hybridization, Oxalobacteraceae/*classification/genetics/isolation & purification, Phospholipids/chemistry, *Phylogeny, Putrescine/chemistry, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/chemistry
- topic2: Transcriptome
Reference
@id | authors | title | doi/url | journal | pubmed |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
43867 | Khulan Altankhuu, Jaisoo Kim | Massilia solisilvae sp. nov., Massilia terrae sp. nov. and Massilia agilis sp. nov., isolated from forest soil in South Korea by using a newly developed culture method | 10.1099/ijsem.0.002076 | IJSEM 67: 3026-3032 2017 | 28820094 |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ |