Strain identifier

BacDive ID: 140970

Type strain: Yes

Species: Marinomonas epiphytica

Strain Designation: SAB-3

Strain history: S. Krishnamurthi; MTCC, India; SAB-3.

NCBI tax ID(s): 1779332 (species)

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General

@ref: 43862

BacDive-ID: 140970

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, motile, rod-shaped, colony-forming

description: Marinomonas epiphytica SAB-3 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from surface of seaweed , collected from the intertidal region of the coastal waters of Anjuna beach, west coast of India, state of Goa.

NCBI tax id

  • NCBI tax id: 1779332
  • Matching level: species

strain history

  • @ref: 67770
  • history: S. Krishnamurthi; MTCC, India; SAB-3.

doi: 10.13145/bacdive140970.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Oceanospirillales
  • family: Oceanospirillaceae
  • genus: Marinomonas
  • species: Marinomonas epiphytica
  • full scientific name: Marinomonas epiphytica Ojha et al. 2017

@ref: 43862

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Oceanospirillales

family: Oceanospirillaceae

genus: Marinomonas

species: Marinomonas epiphytica

strain designation: SAB-3

type strain: yes

Morphology

cell morphology

  • @ref: 43862
  • gram stain: negative
  • cell length: 1.0-1.2 µm
  • cell width: 0.2-0.4 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 43862
  • colony color: ivory
  • colony shape: circular
  • incubation period: 1 day
  • medium used: Marine agar

Culture and growth conditions

culture medium

  • @ref: 43862
  • name: Marine agar (MA)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
43862positiveoptimum25-30mesophilic
43862positivegrowth15-35
67770positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
43862positiveoptimum7
43862positivegrowth5.0-9.0alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 43862
  • oxygen tolerance: aerobe

spore formation

  • @ref: 43862
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
43862NaClpositivegrowth0-6 %(w/v)
43862NaClpositiveoptimum2 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4386216004(R)-lactate-carbon source
4386215588D-malate-carbon source
4386215589L-malate-carbon source
43862167632-oxobutanoate-carbon source
43862309162-oxoglutarate-carbon source
43862739183-O-methyl-D-glucose-carbon source
43862181014-hydroxyphenylacetic acid-carbon source
4386213705acetoacetate-carbon source
43862645522-hydroxybutyrate-carbon source
438628295beta-hydroxybutyrate-carbon source
4386273706bromosuccinate-carbon source
4386217057cellobiose-carbon source
4386218333D-arabitol-carbon source
4386229990D-aspartate-carbon source
4386215824D-fructose-carbon source
4386278697D-fructose 6-phosphate-carbon source
4386228847D-fucose-carbon source
4386212936D-galactose-carbon source
4386218024D-galacturonic acid-carbon source
438628391D-gluconate-carbon source
4386214314D-glucose 6-phosphate-carbon source
4386215748D-glucuronate-carbon source
4386216024D-mannose-carbon source
4386216523D-serine-carbon source
4386217924D-sorbitol-carbon source
4386215740formate-carbon source
4386216537galactarate-carbon source
4386228066gentiobiose-carbon source
4386217754glycerol-carbon source
4386217596inosine-carbon source
4386216977L-alanine-carbon source
4386216467L-arginine-carbon source
4386218287L-fucose-carbon source
4386253071L-galactonate-carbon source
4386215971L-histidine-carbon source
4386262345L-rhamnose-carbon source
4386217115L-serine-carbon source
4386229864mannitol-carbon source
4386228053melibiose-carbon source
4386274611methyl (R)-lactate-carbon source
43862320055methyl beta-D-glucopyranoside-carbon source
4386251850methyl pyruvate-carbon source
4386217268myo-inositol-carbon source
4386263154N-acetyl-beta-D-mannosamine-carbon source
4386228037N-acetylgalactosamine-carbon source
43862506227N-acetylglucosamine-carbon source
4386235418n-acetylneuraminate-carbon source
4386217309pectin-carbon source
4386217272propionate-carbon source
43862576065-oxoproline-carbon source
4386226490quinate-carbon source
4386216634raffinose-carbon source
4386217814salicin-carbon source
4386275228sodium lactate-carbon source
4386217164stachyose-carbon source
4386217992sucrose-carbon source
4386227082trehalose-carbon source
4386232528turanose-carbon source
4386228017starch-hydrolysis
4386217632nitrate-reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.group IDis resistantresistance conc.
43862161680aztreonamyesyes
4386271321fusidateyesyes
4386232735guanidinium chlorideyesyes
4386248607lithium chlorideyesyes
4386250694minocyclineyesyes
4386275273niaproofyesyes
4386275229sodium bromateyesyes
4386264103sodium butyrateyesyes
4386275198tetrazolium blueyesyes
4386275193tetrazolium violetyesyes
4386245735troleandomycinyesyes
438622637amikacinyesyes30 µg (disc)
4386228971ampicillinyesyes10 µg (disc)
438622676amoxicillinyesyes10 µg (disc)
438623493cefoperazoneyesyes75 µg (disc)
438623508ceftazidimeyesyes30 µg (disc)
4386249566cloxacillinyesyes1 µg (disc)
4386229007ceftriaxoneyesyes30 µg (disc)
4386217698chloramphenicolyesyes30 µg (disc)
43862100241ciprofloxacinyesyes5 µg (disc)
4386248923erythromycinyesyes15 µg (disc)
4386217833gentamicinyesyes10 µg (disc)
43862100147nalidixic acidyesyes10 µg (disc)
4386271415nitrofurantoinyesyes300 µg (disc)
43862100246norfloxacinyesyes10 µg (disc)
4386217334penicillinyesyes10 Unit (disc)
4386228864tobramycinyesyes10 µg (disc)
4386228001vancomycinyesyes30 µg (disc)
4386245924trimethoprimyes26yes25 µg (disc)
438629332sulfamethoxazoleyes26yes25 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
4386215688acetoinno
4386216136hydrogen sulfideno
4386235581indoleno

enzymes

@refvalueactivityec
43862catalase+1.11.1.6
43862cytochrome oxidase-1.9.3.1
43862arginine dihydrolase-3.5.3.6
43862ornithine decarboxylase-4.1.1.17
43862lysine decarboxylase-4.1.1.18

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43862C16:0 anteiso0.2
    43862C17:0 anteiso0.2
    43862C10:02.5
    43862C10:0 3OH7.3
    43862C12:02.5
    43862C12:0 3OH0.2
    43862C12:1 3OH0.2
    43862C14:00.5
    43862C16:011.9
    43862C16:1ω7c / C16:1ω6c19.7
    43862C18:04.9
    43862C18:1ω7c / C18:1ω6c47.6
    43862C20:1ω7c0.5
  • type of FA analysis: whole cell analysis
  • incubation medium: Marine agar
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 2
  • software version: Sherlock 6.1
  • library/peak naming table: RTSBA6
  • system: MIS MIDI
  • instrument: Agilent 7890 gas chromatograph
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture durationenrichment culture temperaturehost species
43862surface of seaweed (Dictyota sp.), collected from the intertidal region of the coastal waters of Anjuna beach, west coast of India, state of GoaAnjuna beach, state of Goa, west coast of IndiaIndiaINDAsia15.578873.7384Zobell marine agar plates2-4 weeks30
67770Brown macro algae (Dictyota sp.) growing in the Arabian seaGoaIndiaINDAsiaDictyota sp.

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Coast
#Host#Algae#Brown Algae

Sequence information

16S sequences

  • @ref: 43862
  • description: 16S rRNA gene sequence
  • accession: LN999519
  • database: nuccore

GC content

@refGC-contentmethod
4386241Thermal denaturation, fluorometry
6777041thermal denaturation, midpoint method (Tm)

External links

@ref: 43862

culture collection no.: JCM 31365, KCTC 52293, MTCC 12569

literature

  • topic: Phylogeny
  • Pubmed-ID: 28771118
  • title: Marinomonas epiphytica sp. nov., isolated from a marine intertidal macroalga.
  • authors: Ojha AK, Verma A, Pal Y, Bhatt D, Mayilraj S, Krishnamurthi S
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002014
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, India, Marinomonas/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Phosphatidylethanolamines/chemistry, Phosphatidylglycerols/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seaweed/*microbiology, Sequence Analysis, DNA, Ubiquinone/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43862Anup Kumar Ojha, Ashish Verma, Yash Pal, Deepak Bhatt, Shanmugam Mayilraj, Srinivasan KrishnamurthiMarinomonas epiphytica sp. nov., isolated from a marine intertidal macroalga10.1099/ijsem.0.002014IJSEM 67: 2746-2751 201728771118
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/