Strain identifier

BacDive ID: 140961

Type strain: Yes

Species: Bacillus pacificus

Strain Designation: EB422

Strain history: <- Yang Liu, the Third institute of State Oceanic Administration(SOA)

NCBI tax ID(s): 2026187 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
version 6 (current version):
version 5:
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@ref: 43860

BacDive-ID: 140961

keywords: Gram-positive, spore-forming, facultative anaerobe, Bacteria, 16S sequence, genome sequence, rod-shaped, colony-forming

description: Bacillus pacificus EB422 is a facultative anaerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from sediment of the Pacific Ocean.

NCBI tax id

  • NCBI tax id: 2026187
  • Matching level: species

strain history: <- Yang Liu, the Third institute of State Oceanic Administration(SOA)

doi: 10.13145/bacdive140961.20211221.6

Name and taxonomic classification


  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/firmicutes
  • domain: Bacteria
  • phylum: Firmicutes
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Bacillus
  • species: Bacillus pacificus
  • full scientific name: Bacillus pacificus Liu et al. 2017

@ref: 43860

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Bacillaceae

genus: Bacillus

species: Bacillus pacificus

strain designation: EB422

type strain: yes


cell morphology

@refgram staincell lengthcell widthcell shapemotility
43860positive3.0-4.0 µm1.2-1.6 µmrod-shapedno

colony morphology

  • @ref: 43860
  • colony size: 2-3 mm
  • colony color: white
  • colony shape: circular
  • incubation period: 2 days
  • medium used: Luria-Bertani agar

Culture and growth conditions

culture medium

43860LB (Luria-Bertani) MEDIUMyes
43860Trypticase soy agaryes

culture temp


culture pH


Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
43860facultative anaerobe

spore formation

  • @ref: 43860
  • spore description: central elliptical endospore
  • type of spore: endospore
  • spore formation: yes


@refsaltgrowthtested relationconcentration
43860NaClpositivegrowth0-9 %(w/v)
43860NaClpositiveoptimum1 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
43860168082-dehydro-D-gluconate-builds acid from
43860581435-dehydro-D-gluconate-builds acid from
4386027613amygdalin-builds acid from
4386022599arabinose-builds acid from
4386017057cellobiose-builds acid from
4386015963ribitol-builds acid from
4386017108D-arabinose-builds acid from
4386018333D-arabitol-builds acid from
4386028847D-fucose-builds acid from
4386012936D-galactose-builds acid from
4386062318D-lyxose-builds acid from
4386016899D-mannitol-builds acid from
4386016024D-mannose-builds acid from
4386017924D-sorbitol-builds acid from
4386016443D-tagatose-builds acid from
4386016813galactitol-builds acid from
4386017113erythritol-builds acid from
4386028066gentiobiose-builds acid from
4386017234glucose-builds acid from
4386017754glycerol-builds acid from
4386028087glycogen-builds acid from
4386017268myo-inositol-builds acid from
4386015443inulin-builds acid from
4386018403L-arabitol-builds acid from
4386018287L-fucose-builds acid from
4386062345L-rhamnose-builds acid from
4386017266L-sorbose-builds acid from
4386065328L-xylose-builds acid from
4386017716lactose-builds acid from
4386029864mannitol-builds acid from
438606731melezitose-builds acid from
4386028053melibiose-builds acid from
43860320061methyl alpha-D-glucopyranoside-builds acid from
4386043943methyl alpha-D-mannoside-builds acid from
4386074863methyl beta-D-xylopyranoside-builds acid from
4386032032potassium gluconate-builds acid from
4386016634raffinose-builds acid from
4386026546rhamnose-builds acid from
4386017814salicin-builds acid from
4386030911sorbitol-builds acid from
4386028017starch-builds acid from
4386017992sucrose-builds acid from
4386032528turanose-builds acid from
4386017151xylitol-builds acid from
4386018305arbutin+builds acid from
4386015824D-fructose+builds acid from
4386017634D-glucose+builds acid from
4386016988D-ribose+builds acid from
4386065327D-xylose+builds acid from
43860esculin ferric citrate+builds acid from
4386017306maltose+builds acid from
43860506227N-acetylglucosamine+builds acid from
4386017992sucrose+builds acid from
4386027082trehalose+builds acid from
4386016947citrate+carbon source
43860skimmed milk+hydrolysis

metabolite production

4386016136hydrogen sulfideno

metabolite tests

  • @ref: 43860
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +


43860cytochrome oxidase+
43860arginine dihydrolase+
43860lysine decarboxylase-
43860ornithine decarboxylase-
43860tryptophan deaminase-

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43860C13:0 anteiso2
    43860C15:0 anteiso6.7
    43860C17:0 anteiso2.7
    43860C16:1ω7c / C15:0 iso 2OH8
    43860C12:0 iso1.2
    43860C13:0 iso6.4
    43860C14:0 iso3.5
    43860C15:0 iso13.8
    43860C16:0 iso5.3
    43860C17:0 iso5.2
    43860C17:1 iso I /C17:1 anteiso B4.6
    43860C17:1 iso ω10c1.7
    43860C17:1 iso ω5c1
  • type of FA analysis: whole cell analysis
  • incubation medium: Tripticase soy agar
  • agar/liquid: agar
  • incubation temperature: 28
  • software version: Sherlock 6.0B
  • library/peak naming table: TSBA 6.0
  • system: MIS MIDI
  • instrument: Agilent 6850 gas chromatograph
  • cutoff value:

Isolation, sampling and environmental information


@refsample typegeographic locationcountry
43860sediment of the Pacific Ocean
67771From bottom seawater, sedimentthe Pacific OceanPacific Ocean

isolation source categories


Sequence information

16S sequences

  • @ref: 43860
  • description: 16S rRNA gene sequence
  • accession: KJ812450
  • database: nuccore

Genome sequences

  • @ref: 66792
  • description: Bacillus pacificus EB422
  • accession: GCA_001884025
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 2026187

GC content

  • @ref: 43860
  • GC-content: 35.2
  • method: genome sequence analysis

External links

@ref: 43860

culture collection no.: MCCC 1A06182, KCTC 33858, CIP 111542, NBRC 113915


20215D.Gleim, M.Kracht, N.Weiss et. al.Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
43860Yang Liu, Juan Du, Qiliang Lai, Runying Zeng, Dezan Ye, Jun Xu, Zongze ShaoProposal of nine novel species of the Bacillus cereus group10.1099/ijsem.0.001821IJSEM 67: 2499-2508 201728792367
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67771Curators of the KCTC