Strain identifier

BacDive ID: 140941

Type strain: Yes

Species: Algoriphagus confluentis

Strain Designation: HJM-2

Strain history: <- Jung-Hoon Yoon, Sungkyunkwan Univ.

NCBI tax ID(s): 1697556 (species)

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General

@ref: 43832

BacDive-ID: 140941

keywords: 16S sequence, Bacteria, aerobe, Gram-negative, rod-shaped, colony-forming

description: Algoriphagus confluentis HJM-2 is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from Water which was collected from the junction between the ocean and a freshwater lake at Hwajinpo.

NCBI tax id

  • NCBI tax id: 1697556
  • Matching level: species

strain history

  • @ref: 67771
  • history: <- Jung-Hoon Yoon, Sungkyunkwan Univ.

doi: 10.13145/bacdive140941.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Cytophagia
  • order: Cytophagales
  • family: Cyclobacteriaceae
  • genus: Algoriphagus
  • species: Algoriphagus confluentis
  • full scientific name: Algoriphagus confluentis Park et al. 2016

@ref: 43832

domain: Bacteria

phylum: Bacteroidetes

class: Cytophagia

order: Cytophagales

family: Cyclobacteriaceae

genus: Algoriphagus

species: Algoriphagus confluentis

strain designation: HJM-2

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
43832negative0.5-10 µm0.2-0.5 µmrod-shapedno
67771rod-shapedno
67771negative

colony morphology

  • @ref: 43832
  • colony size: 1-1.5 mm
  • colony color: Glistening, strong orange
  • colony shape: circular
  • incubation period: 5 days
  • medium used: MA agar

pigmentation

  • @ref: 43832
  • production: no
  • name: Flexirubin

Culture and growth conditions

culture medium

  • @ref: 43832
  • name: Marine agar (MA)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
43832positivegrowth10-40
43832positiveoptimum30mesophilic
67771positivegrowth30mesophilic

culture pH

@refabilitytypepH
43832positivegrowth6-8
43832positiveoptimum7-8

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
43832aerobe
67771aerobe

halophily

@refsaltgrowthtested relationconcentration
43832NaClpositivegrowth0-6 %(w/v)
43832NaClpositiveoptimum1-2 %(w/v)

observation

  • @ref: 67771
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4383216899D-mannitol-builds acid from
4383216024D-mannose-builds acid from
4383216988D-ribose-builds acid from
4383217924D-sorbitol-builds acid from
4383265327D-xylose-builds acid from
4383230849L-arabinose-builds acid from
4383262345L-rhamnose-builds acid from
438326731melezitose-builds acid from
4383228053melibiose-builds acid from
4383217268myo-inositol-builds acid from
4383216634raffinose-builds acid from
4383230089acetate-carbon source
4383216150benzoate-carbon source
4383216947citrate-carbon source
4383212936D-galactose-carbon source
4383215740formate-carbon source
4383230849L-arabinose-carbon source
4383229985L-glutamate-carbon source
4383215589L-malate-carbon source
4383215361pyruvate-carbon source
4383230031succinate-carbon source
4383230089acetate-energy source
4383216150benzoate-energy source
4383216947citrate-energy source
4383212936D-galactose-energy source
4383215740formate-energy source
4383230849L-arabinose-energy source
4383229985L-glutamate-energy source
4383215589L-malate-energy source
4383215361pyruvate-energy source
4383230031succinate-energy source
438324853esculin-hydrolysis
4383217368hypoxanthine-hydrolysis
4383217895L-tyrosine-hydrolysis
4383253423tween 40-hydrolysis
4383253425tween 60-hydrolysis
4383253426tween 80-hydrolysis
4383216199urea-hydrolysis
4383215318xanthine-hydrolysis
4383217632nitrate-reduction
4383218420magnesium(2+)-required for growth
4383217057cellobiose+builds acid from
4383215824D-fructose+builds acid from
4383212936D-galactose+builds acid from
4383217634D-glucose+builds acid from
4383217716lactose+builds acid from
4383217306maltose+builds acid from
4383217992sucrose+builds acid from
4383227082trehalose+builds acid from
4383217057cellobiose+carbon source
4383215824D-fructose+carbon source
4383217634D-glucose+carbon source
4383216024D-mannose+carbon source
4383265327D-xylose+carbon source
4383217306maltose+carbon source
4383217814salicin+carbon source
4383217992sucrose+carbon source
4383227082trehalose+carbon source
4383217057cellobiose+energy source
4383215824D-fructose+energy source
4383217634D-glucose+energy source
4383216024D-mannose+energy source
4383265327D-xylose+energy source
4383217306maltose+energy source
4383217814salicin+energy source
4383217992sucrose+energy source
4383227082trehalose+energy source
43832casein+hydrolysis
438325291gelatin+hydrolysis
4383228017starch+hydrolysis
4383253424tween 20+hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
438323393carbenicillinyesyes100 µg (disc)
438323542cephalothinyesyes30 µg (disc)
4383217698chloramphenicolyesyes100 µg (disc)
438326472lincomycinyesyes15 µg (disc)
4383228368novobiocinyesyes5 µg (disc)
4383216869oleandomycinyesyes15 µg (disc)
4383227902tetracyclineyesyes30 µg (disc)
4383218208penicillin gyesyes20 Unit (disc)
4383228971ampicillinyesyes10 µg (disc)
4383217833gentamicinyesyes30 µg (disc)
438326104kanamycinyesyes30 µg (disc)
438327507neomycinyesyes30 µg (disc)
4383217076streptomycinyesyes50 µg (disc)
438328309polymyxin byesyes100 Unit (disc)

enzymes

@refvalueactivityec
43832catalase+1.11.1.6
43832cytochrome oxidase+1.9.3.1
43832alkaline phosphatase+3.1.3.1
43832esterase (C 4)+
43832esterase Lipase (C 8)+
43832leucine arylamidase+3.4.11.1
43832valine arylamidase+
43832cystine arylamidase+3.4.11.3
43832trypsin+3.4.21.4
43832alpha-chymotrypsin+3.4.21.1
43832acid phosphatase+3.1.3.2
43832naphthol-AS-BI-phosphohydrolase+
43832beta-galactosidase+3.2.1.23
43832alpha-glucosidase+3.2.1.20
43832N-acetyl-beta-glucosaminidase+3.2.1.52
43832alpha-galactosidase+/-3.2.1.22
43832beta-glucosidase+/-3.2.1.21
43832alpha-mannosidase+/-3.2.1.24
43832lipase (C 14)-
43832beta-glucuronidase-3.2.1.31
43832alpha-fucosidase-3.2.1.51

fatty acid profile

  • @reffatty acidpercentage
    43832C11:0 anteiso2.9
    43832C15:0 anteiso2.2
    43832C14:0 2OH0.6
    43832C15:1ω6c1
    43832C16:1ω5c1.4
    43832C16:1ω7c / C16:1ω6c19.4
    43832C17:0 2OH1.1
    43832C17:1ω6c3.2
    43832C17:1ω8c0.6
    43832C11:0 iso1
    43832C14:0 iso1.1
    43832C15:0 iso24.8
    43832C15:0 iso 3OH5
    43832C16:0 iso4.8
    43832C16:0 iso 3OH2.1
    43832C16:1 iso H2.2
    43832C17:0 iso0.9
    43832C17:0 iso 3OH10.8
    43832C17:1 iso I / C17:1 anteiso B3.4
    43832C17:1 iso ω9c9.2
  • type of FA analysis: whole cell analysis
  • incubation medium: MA plates
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 3
  • incubation_oxygen: aerobic
  • software version: Sherlock 6.2B
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • instrument: Hewlett Packard 6890
  • method/protocol: Sasser, 1990
  • cutoff value:
  • @reffatty acidpercentage
    43832C11:0 anteiso3.5
    43832C15:0 anteiso3.2
    43832C14:0 2OH0.9
    43832C16:00.6
    43832C16:1ω5c1.1
    43832C16:1ω7c / C16:1ω6c15.1
    43832C17:0 2OH2.3
    43832C17:1ω6c1.9
    43832C11:0 iso1.3
    43832C13:0 iso 3OH1.1
    43832C14:0 iso0.6
    43832C15:0 iso21.6
    43832C15:0 iso 3OH6.3
    43832C15:1 iso2.7
    43832C16:0 iso4.7
    43832C16:0 iso 3OH2.1
    43832C16:1 iso H1.7
    43832C17:0 iso1.7
    43832C17:0 iso 3OH12.6
    43832C17:1 iso I / C17:1 anteiso B3.3
    43832C17:1 iso ω9c7.6
  • type of FA analysis: whole cell analysis
  • incubation medium: MA plates
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 5
  • incubation_oxygen: aerobic
  • software version: Sherlock 6.2B
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • instrument: Hewlett Packard 6890
  • method/protocol: Sasser, 1990
  • cutoff value:
  • @reffatty acidpercentage
    43832C11:0 anteiso4
    43832C15:0 anteiso2.1
    43832C15:0 2OH0.6
    43832C15:1ω6c0.7
    43832C16:0 3OH0.7
    43832C16:1ω5c1.7
    43832C16:1ω7c / C16:1ω6c23.5
    43832C17:0 2OH1.2
    43832C17:1ω6c2.4
    43832C11:0 iso1.5
    43832C14:0 iso0.8
    43832C15:0 iso23.2
    43832C15:0 iso 3OH7.7
    43832C15:1 iso0.7
    43832C16:0 iso3.5
    43832C16:0 iso 3OH2.4
    43832C16:1 iso H1.8
    43832C17:0 iso0.8
    43832C17:0 iso 3OH10.6
    43832C17:1 iso I / C17:1 anteiso B2.6
    43832C17:1 iso ω9c5.9
  • type of FA analysis: whole cell analysis
  • incubation medium: Ma plates
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 7
  • incubation_oxygen: aerobic
  • software version: Sherlock 6.2B
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • instrument: Hewlett Packard 6890
  • method/protocol: Sasser, 1990
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
43832Water which was collected from the junction between the ocean and a freshwater lake at HwajinpoGoseong County, GangwonRepublic of KoreaKORAsia38.4803128.438
67771From water of the confluence of ocean and freshwater lake at HwajinpoRepublic of KoreaKORAsia38.4803128.438

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Freshwater
#Environmental#Aquatic#Lake (large)
#Environmental#Aquatic#Marine

Sequence information

16S sequences

  • @ref: 43832
  • description: 16S rRNA gene sequence
  • accession: KR868708
  • database: nuccore

GC content

@refGC-contentmethod
4383245high performance liquid chromatography (HPLC)
6777145.0

External links

@ref: 43832

culture collection no.: KCTC 42704, NBRC 111222

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny26475126Algoriphagus confluentis sp. nov., isolated from the junction between the ocean and a freshwater lake.Park S, Kim S, Jung YT, Yoon JHInt J Syst Evol Microbiol10.1099/ijsem.0.0006862015Bacterial Typing Techniques, Bacteroidetes/classification/genetics/isolation & purification, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Lakes/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Oceans and Seas, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Republic of Korea, Seawater/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryGenetics
Phylogeny31935174Algoriphagus kandeliae sp. nov., isolated from mangrove rhizosphere soil.Song ZM, Wang KL, Yin Q, Chen CC, Xu YInt J Syst Evol Microbiol10.1099/ijsem.0.0039542020Bacterial Typing Techniques, Bacteroidetes/*classification/isolation & purification, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hong Kong, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhizophoraceae/*microbiology, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43832Sooyeon Park, Sona Kim, Yong-Taek Jung, Jung-Hoon YoonAlgoriphagus confluentis sp. nov., isolated from the junction between the ocean and a freshwater lake10.1099/ijsem.0.000686IJSEM 66: 118-124 201626475126
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc