Strain identifier

BacDive ID: 140932

Type strain: Yes

Species: Paenibacillus nasutitermitis

Strain Designation: P5-1

NCBI tax ID(s): 1652958 (species)

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General

@ref: 43812

BacDive-ID: 140932

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive, rod-shaped, colony-forming

description: Paenibacillus nasutitermitis P5-1 is an aerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from The hindgut of a wood-feeding higher termite, Nasutitermes sp., collected from Hainan.

NCBI tax id

  • NCBI tax id: 1652958
  • Matching level: species

doi: 10.13145/bacdive140932.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Paenibacillus
  • species: Paenibacillus nasutitermitis
  • full scientific name: Paenibacillus nasutitermitis Wang et al. 2016

@ref: 43812

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Paenibacillaceae

genus: Paenibacillus

species: Paenibacillus nasutitermitis

strain designation: P5-1

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
43812positive2.2-3.5 µm0.5-0.7 µmrod-shapedno
69480yes97.789
69480positive100

colony morphology

  • @ref: 43812
  • colony color: Transparent
  • colony shape: circular
  • incubation period: 2 days
  • medium used: TSB agar

Culture and growth conditions

culture medium

  • @ref: 43812
  • name: TSB agar
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
43812positivegrowth10-40
43812positiveoptimum30mesophilic

culture pH

@refabilitytypepHPH range
43812positivegrowth5.5-9alkaliphile
43812positiveoptimum7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 43812
  • oxygen tolerance: aerobe

spore formation

@refspore descriptiontype of sporespore formationconfidence
43812terminalendosporeyes
69481yes100
69480yes100

halophily

@refsaltgrowthtested relationconcentrationhalophily level
43812NaClpositivegrowth0-4 %
43812NaClpositiveoptimum0 %non-halophilic

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4381217128adipate-assimilation
4381227689decanoate-assimilation
4381216947citrate-assimilation
4381216024D-mannose-assimilation
4381225115malate-assimilation
4381230849L-arabinose-assimilation
43812506227N-acetylglucosamine-assimilation
4381218401phenylacetate-assimilation
43812581435-dehydro-D-gluconate-builds acid from
4381215824D-fructose-builds acid from
4381212936D-galactose-builds acid from
4381216024D-mannose-builds acid from
4381230849L-arabinose-builds acid from
4381217814salicin-builds acid from
4381217234glucose-fermentation
43812casein-hydrolysis
438125291gelatin-hydrolysis
4381228017starch-hydrolysis
4381253426tween 80-hydrolysis
4381217632nitrate-reduction
4381217634D-glucose+assimilation
4381216899D-mannitol+assimilation
4381217306maltose+assimilation
4381227613amygdalin+builds acid from
4381217057cellobiose+builds acid from
4381217634D-glucose+builds acid from
4381216899D-mannitol+builds acid from
4381216988D-ribose+builds acid from
4381265327D-xylose+builds acid from
438124853esculin+builds acid from
4381228066gentiobiose+builds acid from
4381228087glycogen+builds acid from
4381217306maltose+builds acid from
438126731melezitose+builds acid from
4381228053melibiose+builds acid from
4381274863methyl beta-D-xylopyranoside+builds acid from
4381217992sucrose+builds acid from
438124853esculin+hydrolysis
4381217716lactose+/-builds acid from
43812320061methyl alpha-D-glucopyranoside+/-builds acid from
4381243943methyl alpha-D-mannoside+/-builds acid from
4381216634raffinose+/-builds acid from
4381227082trehalose+/-builds acid from

metabolite production

@refChebi-IDmetaboliteproduction
4381215688acetoinno
4381235581indoleno
4381216136hydrogen sulfideyes

metabolite tests

  • @ref: 43812
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
43812catalase+1.11.1.6
43812cytochrome oxidase+1.9.3.1
43812urease+3.5.1.5
43812arginine dihydrolase+3.5.3.6
43812esterase Lipase (C 8)+
43812esterase (C 4)+
43812chymotrypsin+3.4.4.5
43812naphthol-AS-BI-phosphohydrolase+
43812alpha-galactosidase+3.2.1.22
43812beta-galactosidase+3.2.1.23
43812alpha-glucosidase+3.2.1.20
43812N-acetyl-beta-glucosaminidase+3.2.1.52
43812beta-D-fucosidase+3.2.1.38

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43812C15:0 anteiso53.9
    43812C17:0 anteiso1.4
    43812C14:02.6
    43812C16:08.5
    43812C14:0 iso3.9
    43812C15:0 iso13.4
    43812C16:0 iso8
  • type of FA analysis: whole cell analysis
  • incubation medium: TSB
  • agar/liquid: liquid
  • incubation temperature: 30
  • incubation time: 2
  • incubation_oxygen: aerobic
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • instrument: Agilent 6890N
  • cutoff value:

Isolation, sampling and environmental information

isolation

  • @ref: 43812
  • sample type: The hindgut of a wood-feeding higher termite, Nasutitermes sp., collected from Hainan
  • geographic location: Hainan
  • country: China
  • origin.country: CHN
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Host#Arthropoda#Insecta
#Host Body-Site#Gastrointestinal tract#Stomach

Sequence information

16S sequences

  • @ref: 43812
  • description: 16S rRNA gene sequence
  • accession: KR611320
  • database: nuccore

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Paenibacillus nasutitermitis CGMCC 1.15178GCA_014641075contigncbi1652958
66792Paenibacillus nasutitermitis strain CGMCC 1.151781652958.3wgspatric1652958

GC content

  • @ref: 43812
  • GC-content: 48.9
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileyes81.097yes
gram-positiveyes86.84yes
anaerobicno99.345no
aerobicyes91.895no
halophileno95.194no
spore-formingyes95.144no
thermophileno99.418yes
glucose-utilyes90.141yes
flagellatedyes65.556yes
glucose-fermentno89.09no

External links

@ref: 43812

culture collection no.: CGMCC 1.15178, NBRC 111536

literature

  • topic: Phylogeny
  • Pubmed-ID: 26620554
  • title: Paenibacillus nasutitermitis sp. nov., isolated from a termite gut.
  • authors: Wang XM, Ma S, Yang SY, Peng R, Zheng Y, Yang H
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.000807
  • year: 2015
  • mesh: Animals, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Glycolipids/analysis, Isoptera/*microbiology, Paenibacillus/*classification/genetics/isolation & purification, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spores, Bacterial, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43812Xue Min Wang, Shichun Ma, Shu Yan Yang, Rong Peng, Ying Zheng, Hong YangPaenibacillus nasutitermitis sp. nov., isolated from a termite gut10.1099/ijsem.0.000807IJSEM 66: 901-905 201626620554
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1