Strain identifier

BacDive ID: 140896

Type strain: Yes

Species: Membranihabitans marinus

Strain Designation: CZ-AZ5

Strain history: Z.-P. Liu CZ-AZ5.

NCBI tax ID(s): 1227546 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 43729

BacDive-ID: 140896

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, heterotroph, Gram-negative, rod-shaped, colony-forming

description: Membranihabitans marinus CZ-AZ5 is an obligate aerobe, heterotroph, Gram-negative bacterium that forms circular colonies and was isolated from A biofilm of a biological filter in a recirculating aquaculture system.

NCBI tax id

  • NCBI tax id: 1227546
  • Matching level: species

strain history

  • @ref: 67770
  • history: Z.-P. Liu CZ-AZ5.

doi: 10.13145/bacdive140896.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Saprospiria
  • order: Saprospirales
  • family: Saprospiraceae
  • genus: Membranihabitans
  • species: Membranihabitans marinus
  • full scientific name: Membranihabitans marinus (Li et al. 2016) Deshmukh and Oren 2022
  • synonyms

    • @ref: 20215
    • synonym: Membranicola marinus

@ref: 43729

domain: Bacteria

phylum: Bacteroidetes

class: Sphingobacteriia

order: Saprospirales

family: Saprospiraceae

genus: Membranicola

species: Membranicola marinus

strain designation: CZ-AZ5

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
43729negative3-3.7 µm0.6-0.7 µmrod-shapedno
69480negative98.124

colony morphology

  • @ref: 43729
  • colony size: 0.5-1 mm
  • colony color: orange, opaque
  • colony shape: circular
  • incubation period: 7 days
  • medium used: MA agar

pigmentation

  • @ref: 43729
  • production: no
  • name: Flexirubin-type

Culture and growth conditions

culture medium

  • @ref: 43729
  • name: Marine agar (MA)
  • growth: yes

culture temp

@refgrowthtypetemperature
43729positivegrowth20-40
43729positiveoptimum30
67770positivegrowth30

culture pH

@refabilitytypepHPH range
43729positivegrowth6-8.5alkaliphile
43729positiveoptimum7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 43729
  • oxygen tolerance: obligate aerobe

nutrition type

  • @ref: 43729
  • type: heterotroph

spore formation

@refspore formationconfidence
43729no
69480no96.572

halophily

@refsaltgrowthtested relationconcentration
43729NaClpositivegrowth0-5 %(w/v)
43729NaClpositiveoptimum1 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4372917234glucose-fermentation
4372917057cellobiose-growth
4372916947citrate-growth
4372912936D-galactose-growth
4372916024D-mannose-growth
4372965327D-xylose-growth
4372925115malate-growth
4372929806fumarate-growth
4372917234glucose-growth
4372915428glycine-growth
4372917716lactose-growth
4372917306maltose-growth
4372929864mannitol-growth
4372915361pyruvate-growth
4372930031succinate-growth
4372929016arginine-hydrolysis
43729casein-hydrolysis
437295291gelatin-hydrolysis
4372928017starch-hydrolysis
4372953426tween 80-hydrolysis
4372916199urea-hydrolysis
4372917632nitrate-reduction
4372917924D-sorbitol+growth
4372929985L-glutamate+growth
4372917309pectin+growth
4372927082trehalose+growth
437294853esculin+hydrolysis
4372953424tween 20+hydrolysis
4372953423tween 40+hydrolysis
4372953425tween 60+hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
437293745clindamycinyesyes2 µg (disc)
4372917698chloramphenicolyesyes30 µg (disc)
4372928971ampicillinyesyes10 µg (disc)
437299332sulfamethoxazoleyesyes300 µg (disc)
4372948923erythromycinyesyes15 µg (disc)
4372928001vancomycinyesyes30 µg (disc)
4372927902tetracyclineyesyes30 µg (disc)
4372917833gentamicinyesyes10 µg (disc)
4372917076streptomycinyesyes10 µg (disc)
4372918208penicillin gyesyes10 Unit (disc)
437297809oxacillinyesyes1 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
4372935581indoleno
4372916136hydrogen sulfideyes

enzymes

@refvalueactivityec
43729catalase+1.11.1.6
43729cytochrome oxidase-1.9.3.1
43729alkaline phosphatase+3.1.3.1
43729esterase Lipase (C 8)+
43729leucine arylamidase+3.4.11.1
43729valine arylamidase+
43729cystine arylamidase+3.4.11.3
43729acid phosphatase+3.1.3.2
43729naphthol-AS-BI-phosphohydrolase+
43729alpha-galactosidase+3.2.1.22
43729beta-galactosidase+3.2.1.23
43729alpha-glucosidase+3.2.1.20
43729N-acetyl-beta-glucosaminidase+3.2.1.52
43729beta-glucosidase+3.2.1.21
43729alpha-mannosidase+3.2.1.24
43729alpha-fucosidase+3.2.1.51
43729esterase (C 4)-
43729lipase (C 14)-
43729trypsin-3.4.21.4
43729alpha-chymotrypsin-3.4.21.1
43729beta-glucuronidase-3.2.1.31

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
43729A biofilm of a biological filter in a recirculating aquaculture systemTianjinChinaCHNAsia
67770Biological filter in a marine recirculating aquaculture system in TianjinChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Aquaculture
#Environmental#Biofilm

taxonmaps

  • @ref: 69479
  • File name: preview.99_156551.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_2448;96_59087;97_76482;98_103196;99_156551&stattab=map
  • Last taxonomy: Membranicola marinus subclade
  • 16S sequence: JX306763
  • Sequence Identity:
  • Total samples: 1253
  • soil counts: 35
  • aquatic counts: 1162
  • animal counts: 47
  • plant counts: 9

Sequence information

16S sequences

  • @ref: 43729
  • description: Membranihabitans marinus strain CZ-AZ5 16S ribosomal RNA gene, partial sequence
  • accession: JX306763
  • database: nuccore

Genome sequences

  • @ref: 66792
  • description: Membranihabitans marinus CZ-AZ5
  • accession: GCA_021729155
  • assembly level: scaffold
  • database: ncbi
  • NCBI tax ID: 1227546

GC content

  • @ref: 67770
  • GC-content: 40.1
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno96.514yes
gram-positiveno98.124no
anaerobicno99.112yes
aerobicyes88.076no
halophileno79.094yes
spore-formingno96.572yes
motileno89.688yes
glucose-fermentno89.913yes
thermophileno99.277no
glucose-utilyes80.882yes

External links

@ref: 43729

culture collection no.: CGMCC 1.13179, JCM 18886

literature

  • topic: Phylogeny
  • Pubmed-ID: 26702614
  • title: Membranicola marinus gen. nov., sp. nov., a new member of the family Saprospiraceae isolated from a biofilter in a recirculating aquaculture system.
  • authors: Li X, Liu Y, Chen Z, Liu LZ, Liu ZP, Liu Y
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.000870
  • year: 2015

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43729Xian Li, Ying Liu, Zhu Chen, Liang-Zi Liu, Zhi-Pei Liu. Ying LiuMembranicola marinus gen. nov., sp. nov., a new member of the family Saprospiraceae isolated from a biofilter in a recirculating aquaculture system10.1099/ijsem.0.000870IJSEM 66: 1275-1280 201626702614
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes