Strain identifier

BacDive ID: 140871

Type strain: Yes

Species: Thalassobius litorarius

Strain Designation: MME-075

Strain history: M.-J. Seo; Incheon Natl. Univ., South Korea; MME-075.

NCBI tax ID(s): 1663020 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 8.1 (current version)

General

@ref: 43696

BacDive-ID: 140871

keywords: 16S sequence, Bacteria, aerobe, Gram-negative, rod-shaped, colony-forming

description: Thalassobius litorarius MME-075 is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from Tidal flat samples of Muui-do.

NCBI tax id

  • NCBI tax id: 1663020
  • Matching level: species

strain history

  • @ref: 67770
  • history: M.-J. Seo; Incheon Natl. Univ., South Korea; MME-075.

doi: 10.13145/bacdive140871.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodobacterales
  • family: Roseobacteraceae
  • genus: Thalassobius
  • species: Thalassobius litorarius
  • full scientific name: Thalassobius litorarius Park et al. 2016

  • synonyms

    • @ref: 20215
    • synonym: Thalassovita litoraria

@ref: 43696

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhodobacterales

family: Rhodobacteraceae

genus: Thalassobius

species: Thalassobius litorarius

strain designation: MME-075

type strain: yes

Morphology

cell morphology

  • @ref: 43696
  • gram stain: negative
  • cell length: 1.8-2.2 µm
  • cell width: 0.9-1.1 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 43696
  • colony size: 1 mm
  • colony color: Cream
  • colony shape: circular
  • incubation period: 3 days
  • medium used: MA agar

Culture and growth conditions

culture medium

  • @ref: 43696
  • name: Marine agar (MA)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
43696positivegrowth20-35
43696positiveoptimum25-30mesophilic
67770positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
43696positivegrowth7-9alkaliphile
43696positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 43696
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentrationhalophily level
43696NaClpositivegrowth1-5 %(w/v)
43696NaClpositiveoptimum2 %(w/v)slightly halophilic

observation

  • @ref: 67770
  • observation: quinones: Q-10

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4369617057cellobiose-builds acid from
4369615824D-fructose-builds acid from
4369612936D-galactose-builds acid from
4369617634D-glucose-builds acid from
4369616024D-mannose-builds acid from
4369616988D-ribose-builds acid from
4369665327D-xylose-builds acid from
4369630849L-arabinose-builds acid from
4369662345L-rhamnose-builds acid from
4369628053melibiose-builds acid from
4369627082trehalose-builds acid from
4369616150benzoate-carbon source
4369617057cellobiose-carbon source
4369616947citrate-carbon source
4369612936D-galactose-carbon source
4369617634D-glucose-carbon source
4369616899D-mannitol-carbon source
4369616024D-mannose-carbon source
4369616988D-ribose-carbon source
4369617924D-sorbitol-carbon source
4369665327D-xylose-carbon source
4369615740formate-carbon source
4369617754glycerol-carbon source
4369617268myo-inositol-carbon source
4369630849L-arabinose-carbon source
4369662345L-rhamnose-carbon source
4369617716lactose-carbon source
4369628053melibiose-carbon source
4369616634raffinose-carbon source
4369630031succinate-carbon source
4369627082trehalose-carbon source
4369617234glucose-fermentation
436964853esculin-hydrolysis
436965291gelatin-hydrolysis
4369617895L-tyrosine-hydrolysis
43696O-nitrophenyl-beta-D-galactopyranosid-hydrolysis
4369628017starch-hydrolysis
4369653424tween 20-hydrolysis
4369653423tween 40-hydrolysis
4369653426tween 80-hydrolysis
4369617632nitrate-reduction
4369630089acetate+carbon source
4369615824D-fructose+carbon source
4369625115malate+carbon source
4369629985L-glutamate+carbon source
4369615729L-ornithine+carbon source
4369617306maltose+carbon source
4369615361pyruvate+carbon source
4369617992sucrose+carbon source
43696casein+hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
4369628971ampicillinyesyes10 µg (disc)
436963393carbenicillinyesyes100 µg (disc)
436963542cephalothinyesyes30 µg (disc)
43696100241ciprofloxacinyesyes10 µg (disc)
4369648923erythromycinyesyes25 µg (disc)
4369617833gentamicinyesyes30 µg (disc)
436966104kanamycinyesyes30 µg (disc)
436967507neomycinyesyes30 µg (disc)
43696100246norfloxacinyesyes20 µg (disc)
4369628368novobiocinyesyes10 µg (disc)
4369617076streptomycinyesyes50 µg (disc)
4369627902tetracyclineyesyes30 µg (disc)
4369618208penicillin gyesyes20 Unit (disc)
436968309polymyxin byesyes100 Unit (disc)
436966472lincomycinyesyes15 µg (disc)

metabolite production

  • @ref: 43696
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
43696alkaline phosphatase+3.1.3.1
43696esterase (C 4)+
43696esterase Lipase (C 8)+
43696leucine arylamidase+3.4.11.1
43696urease-3.5.1.5
43696arginine dihydrolase-3.5.3.6
43696lipase (C 14)-
43696valine arylamidase-
43696cystine arylamidase-3.4.11.3
43696trypsin-3.4.21.4
43696alpha-chymotrypsin-3.4.21.1
43696acid phosphatase-3.1.3.2
43696naphthol-AS-BI-phosphohydrolase-
43696alpha-galactosidase-3.2.1.22
43696beta-galactosidase-3.2.1.23
43696beta-glucuronidase-3.2.1.31
43696alpha-glucosidase-3.2.1.20
43696beta-glucosidase-3.2.1.21
43696N-acetyl-beta-glucosaminidase-3.2.1.52
43696alpha-mannosidase-3.2.1.24
43696alpha-fucosidase-3.2.1.51
43696catalase+1.11.1.6
43696cytochrome oxidase+1.9.3.1

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    43696C10:0 3OH5.311425
    43696C12:04.412
    43696C12:0 2OH3.113177
    43696C16:023.816
    43696C16:1ω6c / C16:1ω7c / C15:0 iso 2OH22.615816
    43696C17:0-cyclo14.416888
    43696C18:05.818
    43696C18:1ω6c / C18:1ω7c18.517822
  • type of FA analysis: whole cell analysis
  • incubation medium: MA
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 3
  • incubation_oxygen: aerobic
  • software version: Sherlock 6.2
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • instrument: Agilent Technologies 6890
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
43696Tidal flat samples of Muui-doMuui-do, IncheonRepublic of KoreaKORAsia37.68126.695
67770Tidal flat

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Tidal flat

Sequence information

16S sequences

  • @ref: 43696
  • description: 16S rRNA gene sequence
  • accession: KP410684
  • database: nuccore

GC content

  • @ref: 67770
  • GC-content: 62.1
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 43696

culture collection no.: KCCM 43143, JCM 30758

literature

  • topic: Phylogeny
  • Pubmed-ID: 26821920
  • title: Thalassobius litorarius sp. nov., isolated from a tidal flat.
  • authors: Park I, Cha IT, Seo MJ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.000931
  • year: 2016
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Rhodobacteraceae/*classification/genetics/isolation & purification, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/chemistry
  • topic2: Genetics

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43696Inhye Park, In-Tae Cha, Myung-Ji SeoThalassobius litorarius sp. nov., isolated from a tidal flat10.1099/ijsem.0.000931IJSEM 66: 1666-1672 201626821920
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/