Strain identifier
BacDive ID: 140833
Type strain:
Species: Cupriavidus nantongensis
Strain Designation: X1
NCBI tax ID(s): 1796606 (species)
General
@ref: 43635
BacDive-ID: 140833
keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-negative, motile, coccus-shaped, colony-forming
description: Cupriavidus nantongensis X1 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from sludge collected from the vicinity of a pesticide manufacturer.
NCBI tax id
- NCBI tax id: 1796606
- Matching level: species
doi: 10.13145/bacdive140833.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Burkholderiales
- family: Burkholderiaceae
- genus: Cupriavidus
- species: Cupriavidus nantongensis
- full scientific name: Cupriavidus nantongensis Sun et al. 2016
@ref: 43635
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Burkholderiales
family: Burkholderiaceae
genus: Cupriavidus
species: Cupriavidus nantongensis
strain designation: X1
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | flagellum arrangement | confidence |
---|---|---|---|---|---|---|---|
43635 | negative | 1.3-2.4 µm | 1.3-1.5 µm | coccus-shaped | yes | gliding | |
69480 | yes | 95.423 | |||||
69480 | negative | 99.997 |
colony morphology
- @ref: 43635
- colony size: 0.5-1 mm
- colony color: Pale
- colony shape: circular
- incubation period: 2 days
- medium used: Luria Bertani agar
Culture and growth conditions
culture medium
- @ref: 43635
- name: LB (Luria-Bertani) MEDIUM
- growth: yes
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
43635 | positive | growth | 20-42 | |
43635 | positive | optimum | 30-37 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
43635 | positive | growth | 6-9 | alkaliphile |
43635 | positive | optimum | 7 |
Physiology and metabolism
tolerance
@ref | compound | concentration |
---|---|---|
43635 | Cobalt | 0.05 mg/ml |
43635 | Cobalt | 0.1 mg/ml |
43635 | Zinc | 0.5 mg/ml |
oxygen tolerance
- @ref: 43635
- oxygen tolerance: aerobe
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 99 |
69480 | no | 99.973 |
halophily
- @ref: 43635
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 0-2 %(w/v)
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
43635 | 17634 | D-glucose | - | assimilation |
43635 | 16899 | D-mannitol | - | assimilation |
43635 | 16024 | D-mannose | - | assimilation |
43635 | 30849 | L-arabinose | - | assimilation |
43635 | 17306 | maltose | - | assimilation |
43635 | 506227 | N-acetylglucosamine | - | assimilation |
43635 | 18401 | phenylacetate | - | assimilation |
43635 | 27613 | amygdalin | - | builds acid from |
43635 | 18305 | arbutin | - | builds acid from |
43635 | 15963 | ribitol | - | builds acid from |
43635 | 17108 | D-arabinose | - | builds acid from |
43635 | 18333 | D-arabitol | - | builds acid from |
43635 | 17057 | cellobiose | - | builds acid from |
43635 | 15824 | D-fructose | - | builds acid from |
43635 | 28847 | D-fucose | - | builds acid from |
43635 | 12936 | D-galactose | - | builds acid from |
43635 | 17634 | D-glucose | - | builds acid from |
43635 | 17716 | lactose | - | builds acid from |
43635 | 62318 | D-lyxose | - | builds acid from |
43635 | 17306 | maltose | - | builds acid from |
43635 | 16899 | D-mannitol | - | builds acid from |
43635 | 16024 | D-mannose | - | builds acid from |
43635 | 6731 | melezitose | - | builds acid from |
43635 | 28053 | melibiose | - | builds acid from |
43635 | 16634 | raffinose | - | builds acid from |
43635 | 16988 | D-ribose | - | builds acid from |
43635 | 17924 | D-sorbitol | - | builds acid from |
43635 | 16443 | D-tagatose | - | builds acid from |
43635 | 16551 | D-trehalose | - | builds acid from |
43635 | 32528 | turanose | - | builds acid from |
43635 | 65327 | D-xylose | - | builds acid from |
43635 | 16813 | galactitol | - | builds acid from |
43635 | 17113 | erythritol | - | builds acid from |
43635 | esculin ferric citrate | - | builds acid from | |
43635 | 28066 | gentiobiose | - | builds acid from |
43635 | 28087 | glycogen | - | builds acid from |
43635 | 17268 | myo-inositol | - | builds acid from |
43635 | 15443 | inulin | - | builds acid from |
43635 | 30849 | L-arabinose | - | builds acid from |
43635 | 18403 | L-arabitol | - | builds acid from |
43635 | 18287 | L-fucose | - | builds acid from |
43635 | 62345 | L-rhamnose | - | builds acid from |
43635 | 17266 | L-sorbose | - | builds acid from |
43635 | 320061 | methyl alpha-D-glucopyranoside | - | builds acid from |
43635 | 43943 | methyl alpha-D-mannoside | - | builds acid from |
43635 | 74863 | methyl beta-D-xylopyranoside | - | builds acid from |
43635 | 506227 | N-acetylglucosamine | - | builds acid from |
43635 | potassium 2-dehydro-D-gluconate | - | builds acid from | |
43635 | potassium 5-dehydro-D-gluconate | - | builds acid from | |
43635 | 32032 | potassium gluconate | - | builds acid from |
43635 | 17814 | salicin | - | builds acid from |
43635 | 28017 | starch | - | builds acid from |
43635 | 17992 | sucrose | - | builds acid from |
43635 | 17151 | xylitol | - | builds acid from |
43635 | 17234 | glucose | - | fermentation |
43635 | 4853 | esculin | - | hydrolysis |
43635 | 5291 | gelatin | - | hydrolysis |
43635 | 28017 | starch | - | hydrolysis |
43635 | 16301 | nitrite | - | reduction |
43635 | 27689 | decanoate | + | assimilation |
43635 | 25115 | malate | + | assimilation |
43635 | 32032 | potassium gluconate | + | assimilation |
43635 | 17754 | glycerol | + | builds acid from |
43635 | 53424 | tween 20 | + | hydrolysis |
43635 | 53426 | tween 80 | + | hydrolysis |
43635 | 17632 | nitrate | + | reduction |
43635 | 17128 | adipate | +/- | assimilation |
43635 | 16947 | citrate | +/- | assimilation |
antibiotic resistance
@ref | ChEBI | metabolite | is antibiotic | is sensitive | sensitivity conc. | is resistant | resistance conc. |
---|---|---|---|---|---|---|---|
43635 | 3534 | cephalexin | yes | yes | 30 µg (disc) | ||
43635 | 18208 | penicillin g | yes | yes | 10 µg (disc) | ||
43635 | 17076 | streptomycin | yes | yes | 10 µg (disc) | ||
43635 | 2676 | amoxicillin | yes | yes | 20 µg (disc) |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
43635 | 15688 | acetoin | no |
43635 | 16136 | hydrogen sulfide | no |
43635 | 35581 | indole | yes |
metabolite tests
@ref | Chebi-ID | metabolite | methylred-test | voges-proskauer-test |
---|---|---|---|---|
43635 | 17234 | glucose | - | |
43635 | 15688 | acetoin | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
43635 | catalase | + | 1.11.1.6 |
43635 | cytochrome oxidase | + | 1.9.3.1 |
43635 | alkaline phosphatase | + | 3.1.3.1 |
43635 | esterase (C 4) | + | |
43635 | acid phosphatase | + | 3.1.3.2 |
43635 | leucine arylamidase | + | 3.4.11.1 |
43635 | naphthol-AS-BI-phosphohydrolase | +/- | |
43635 | valine arylamidase | +/- | |
43635 | cystine arylamidase | +/- | 3.4.11.3 |
43635 | lipase (C 14) | - | |
43635 | trypsin | - | 3.4.21.4 |
43635 | alpha-chymotrypsin | - | 3.4.21.1 |
43635 | alpha-galactosidase | - | 3.2.1.22 |
43635 | beta-galactosidase | - | 3.2.1.23 |
43635 | beta-glucuronidase | - | 3.2.1.31 |
43635 | beta-glucosidase | - | 3.2.1.21 |
43635 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
43635 | alpha-mannosidase | - | 3.2.1.24 |
43635 | alpha-fucosidase | - | 3.2.1.51 |
43635 | arginine dihydrolase | - | 3.5.3.6 |
43635 | urease | - | 3.5.1.5 |
Isolation, sampling and environmental information
isolation
- @ref: 43635
- sample type: sludge collected from the vicinity of a pesticide manufacturer
- geographic location: Nantong, Jiangsu Province
- country: China
- origin.country: CHN
- continent: Asia
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Industrial | #Plant (Factory) |
#Environmental | #Terrestrial | #Mud (Sludge) |
taxonmaps
- @ref: 69479
- File name: preview.99_1912.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_12;96_409;97_464;98_534;99_1912&stattab=map
- Last taxonomy: Cupriavidus
- 16S sequence: KU168038
- Sequence Identity:
- Total samples: 2331
- soil counts: 1401
- aquatic counts: 297
- animal counts: 341
- plant counts: 292
Sequence information
16S sequences
- @ref: 43635
- description: 16S rRNA gene sequence
- accession: KU168038
- database: nuccore
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Cupriavidus nantongensis X1 | GCA_001598055 | chromosome | ncbi | 1796606 |
66792 | Cupriavidus nantongensis strain X1 | 1796606.3 | complete | patric | 1796606 |
66792 | Cupriavidus nantongensis strain X1 | 1796606.6 | plasmid | patric | 1796606 |
66792 | Cupriavidus nantongensis X1 | 2687453284 | complete | img | 1796606 |
GC content
- @ref: 43635
- GC-content: 66.6
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 99 | no |
motile | yes | 86.194 | no |
gram-positive | no | 98.287 | yes |
anaerobic | no | 98.996 | no |
aerobic | yes | 93.046 | no |
halophile | no | 96.471 | no |
spore-forming | no | 93.928 | no |
glucose-util | no | 70.614 | yes |
flagellated | no | 63.903 | yes |
thermophile | no | 98.141 | yes |
glucose-ferment | no | 91.187 | no |
External links
@ref: 43635
culture collection no.: KCTC 42909, LMG 29218
literature
- topic: Phylogeny
- Pubmed-ID: 27001671
- title: Cupriavidus nantongensis sp. nov., a novel chlorpyrifos-degrading bacterium isolated from sludge.
- authors: Sun LN, Wang DS, Yang ED, Fang LC, Chen YF, Tang XY, Hua RM
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.001034
- year: 2016
- mesh: Bacterial Typing Techniques, Base Composition, China, Chlorpyrifos/*metabolism, Cupriavidus/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Ubiquinone/chemistry
- topic2: Transcriptome
Reference
@id | authors | title | doi/url | journal | pubmed |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
43635 | Le-Ni Sun, Dao-Sheng Wang, En-Dong Yang, Lian-Cheng Fang, Yi-Fei Chen, Xin-Yun Tang, Ri-Mao Hua | Cupriavidus nantongensis sp. nov., a novel chlorpyrifos-degrading bacterium isolated from sludge | 10.1099/ijsem.0.001034 | IJSEM 66: 2335-2341 2016 | 27001671 |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | ||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 |