Strain identifier

BacDive ID: 14080

Type strain: Yes

Species: Shewanella canadensis

Strain Designation: HAW-EB2

Strain history: CIP <- 2007, J. S. Zhao, Nat. Res. Council Canada, Montreal, Canada: strain HAW-EB2

NCBI tax ID(s): 271096 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 7526

BacDive-ID: 14080

DSM-Number: 18399

keywords: genome sequence, 16S sequence, Bacteria, aerobe, psychrophilic, Gram-negative, motile, rod-shaped

description: Shewanella canadensis HAW-EB2 is an aerobe, psychrophilic, Gram-negative bacterium that was isolated from marine sediment.

NCBI tax id

  • NCBI tax id: 271096
  • Matching level: species

strain history

@refhistory
7526<- J.-S. Zhao, Biotechnol. Res. Inst., Natl. Res. Council of Canada, Montreal; HAW-EB2
117125CIP <- 2007, J. S. Zhao, Nat. Res. Council Canada, Montreal, Canada: strain HAW-EB2

doi: 10.13145/bacdive14080.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Alteromonadales
  • family: Shewanellaceae
  • genus: Shewanella
  • species: Shewanella canadensis
  • full scientific name: Shewanella canadensis Zhao et al. 2007

@ref: 7526

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Alteromonadales

family: Shewanellaceae

genus: Shewanella

species: Shewanella canadensis

full scientific name: Shewanella canadensis Zhao et al. 2007

strain designation: HAW-EB2

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
31886negative2.5 µm0.7 µmrod-shapedyes
117125negativerod-shapedyes

colony morphology

  • @ref: 60227
  • incubation period: 2-3 days

pigmentation

  • @ref: 31886
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7526MODIFIED MEDIUM 514 (DSMZ Medium 1173)yeshttps://mediadive.dsmz.de/medium/1173Name: MODIFIED MEDIUM 514 (DSMZ Medium 1173) Composition: NaCl 39.45 g/l Bacto peptone 10.0 g/l MgCl2 5.9 g/l Yeast extract 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
7526BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium514.pdf
33305Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
117125CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
7526positivegrowth10psychrophilic
31886positivegrowth04-25
31886positiveoptimum10psychrophilic
33305positivegrowth10psychrophilic
60227positivegrowth22psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 60227
  • oxygen tolerance: aerobe

spore formation

  • @ref: 31886
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
31886NaClpositivegrowth01-03 %
31886NaClpositiveoptimum3 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3188630089acetate+carbon source
3188616449alanine+carbon source
3188635391aspartate+carbon source
3188617596inosine+carbon source
3188624996lactate+carbon source
3188625017leucine+carbon source
3188625115malate+carbon source
3188651850methyl pyruvate+carbon source
31886506227N-acetylglucosamine+carbon source
3188626271proline+carbon source
3188617272propionate+carbon source
3188617822serine+carbon source
3188630031succinate+carbon source
3188617748thymidine+carbon source
3188653423tween 40+carbon source
3188653426tween 80+carbon source
3188616704uridine+carbon source
3188631011valerate+carbon source
3188617632nitrate+reduction
11712517632nitrate+reduction
11712516301nitrite-reduction

metabolite production

@refChebi-IDmetaboliteproduction
3188616136hydrogen sulfideyes
11712535581indoleno

enzymes

@refvalueactivityec
31886alkaline phosphatase+3.1.3.1
31886catalase+1.11.1.6
31886gelatinase+
31886cytochrome oxidase+1.9.3.1
31886urease+3.5.1.5
117125oxidase+
117125catalase+1.11.1.6
117125urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
117125-++--+--++++--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling dateisolation date
7526marine sedimentAtlantic Ocean, near Halifax harbour, Emerald BasinCanadaCANNorth America
60227Marine sedimentnear Halifax,Atlantic OceanCanadaCANNorth America2003-06-01
117125Environment, Cold marine sediment, military waste dumping siteEmerald basin, Atlantic Ocean, HalifaxCanadaCANNorth America2003

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Aquatic#Sediment

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
75261Risk group (German classification)
1171251Risk group (French classification)

Sequence information

16S sequences

  • @ref: 7526
  • description: Shewanella canadensis strain HAW-EB2 16S ribosomal RNA gene, partial sequence
  • accession: AY579749
  • length: 1285
  • database: ena
  • NCBI tax ID: 271096

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Shewanella canadensis HAW-EB2GCA_003966225contigncbi271096
66792Shewanella canadensis HAW-EB22902540964draftimg271096

GC content

  • @ref: 31886
  • GC-content: 46.4

External links

@ref: 7526

culture collection no.: DSM 18399, CCUG 54553, NCIMB 14238, CIP 109520

straininfo link

  • @ref: 83249
  • straininfo: 309558

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny17766891Shewanella canadensis sp. nov. and Shewanella atlantica sp. nov., manganese dioxide- and hexahydro-1,3,5-trinitro-1,3,5-triazine-reducing, psychrophilic marine bacteria.Zhao JS, Manno D, Thiboutot S, Ampleman G, Hawari JInt J Syst Evol Microbiol10.1099/ijs.0.64596-02007Atlantic Ocean, Bacterial Typing Techniques, Canada, Carbohydrate Metabolism, Carbon/metabolism, Carboxylic Acids/metabolism, Cell Membrane/chemistry, DNA Gyrase/genetics, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Genes, rRNA, Geologic Sediments/microbiology, Manganese Compounds/*metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Oxidation-Reduction, Oxides/*metabolism, Phylogeny, Quinones/analysis, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Shewanella/chemistry/*classification/isolation & purification/*metabolism, Triazines/*metabolism, beta-Galactosidase/biosynthesisMetabolism
Phylogeny24617134Isolation and characterization of a novel bacterial strain Shewanella marinisediminis sp. nov. from deep-sea sediments.Kim YS, Park JS, Yang HO, Kwon HCJ Environ Biol2013Genes, Bacterial, Geologic Sediments/*microbiology, Marine Biology, Microscopy, Electron, Scanning, Phylogeny, RNA, Ribosomal, 16S/genetics, Shewanella/classification/genetics/*isolation & purification, *Water MicrobiologyEnzymology
Phylogeny34904941Shewanella nanhaiensis sp. nov., a marine bacterium isolated from sediment of South China Sea, and emended descriptions of Shewanella woodyi, Shewanella hanedai and Shewanella canadensis.Cao WR, Li X, Sun YY, Jiang MY, Xu XD, Li YJInt J Syst Evol Microbiol10.1099/ijsem.0.0051522021Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, *Shewanella/classification/isolation & purification, Vitamin K 2/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
7526Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18399)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18399
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31886Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2814628776041
33305Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7273
60227Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 54553)https://www.ccug.se/strain?id=54553
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
83249Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID309558.1StrainInfo: A central database for resolving microbial strain identifiers
117125Curators of the CIPCollection of Institut Pasteur (CIP 109520)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109520