Strain identifier
BacDive ID: 14080
Type strain:
Species: Shewanella canadensis
Strain Designation: HAW-EB2
Strain history: CIP <- 2007, J. S. Zhao, Nat. Res. Council Canada, Montreal, Canada: strain HAW-EB2
NCBI tax ID(s): 271096 (species)
General
@ref: 7526
BacDive-ID: 14080
DSM-Number: 18399
keywords: genome sequence, 16S sequence, Bacteria, aerobe, psychrophilic, Gram-negative, motile, rod-shaped
description: Shewanella canadensis HAW-EB2 is an aerobe, psychrophilic, Gram-negative bacterium that was isolated from marine sediment.
NCBI tax id
- NCBI tax id: 271096
- Matching level: species
strain history
@ref | history |
---|---|
7526 | <- J.-S. Zhao, Biotechnol. Res. Inst., Natl. Res. Council of Canada, Montreal; HAW-EB2 |
117125 | CIP <- 2007, J. S. Zhao, Nat. Res. Council Canada, Montreal, Canada: strain HAW-EB2 |
doi: 10.13145/bacdive14080.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Alteromonadales
- family: Shewanellaceae
- genus: Shewanella
- species: Shewanella canadensis
- full scientific name: Shewanella canadensis Zhao et al. 2007
@ref: 7526
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Alteromonadales
family: Shewanellaceae
genus: Shewanella
species: Shewanella canadensis
full scientific name: Shewanella canadensis Zhao et al. 2007
strain designation: HAW-EB2
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility |
---|---|---|---|---|---|
31886 | negative | 2.5 µm | 0.7 µm | rod-shaped | yes |
117125 | negative | rod-shaped | yes |
colony morphology
- @ref: 60227
- incubation period: 2-3 days
pigmentation
- @ref: 31886
- production: yes
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
7526 | MODIFIED MEDIUM 514 (DSMZ Medium 1173) | yes | https://mediadive.dsmz.de/medium/1173 | Name: MODIFIED MEDIUM 514 (DSMZ Medium 1173) Composition: NaCl 39.45 g/l Bacto peptone 10.0 g/l MgCl2 5.9 g/l Yeast extract 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water |
7526 | BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium514.pdf | |
33305 | Marine agar (MA) | yes | Distilled water make up to (1000.000 ml);Marine agar (55.100 g) | |
117125 | CIP Medium 13 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
7526 | positive | growth | 10 | psychrophilic |
31886 | positive | growth | 04-25 | |
31886 | positive | optimum | 10 | psychrophilic |
33305 | positive | growth | 10 | psychrophilic |
60227 | positive | growth | 22 | psychrophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 60227
- oxygen tolerance: aerobe
spore formation
- @ref: 31886
- spore formation: no
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
31886 | NaCl | positive | growth | 01-03 % |
31886 | NaCl | positive | optimum | 3 % |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
31886 | 30089 | acetate | + | carbon source |
31886 | 16449 | alanine | + | carbon source |
31886 | 35391 | aspartate | + | carbon source |
31886 | 17596 | inosine | + | carbon source |
31886 | 24996 | lactate | + | carbon source |
31886 | 25017 | leucine | + | carbon source |
31886 | 25115 | malate | + | carbon source |
31886 | 51850 | methyl pyruvate | + | carbon source |
31886 | 506227 | N-acetylglucosamine | + | carbon source |
31886 | 26271 | proline | + | carbon source |
31886 | 17272 | propionate | + | carbon source |
31886 | 17822 | serine | + | carbon source |
31886 | 30031 | succinate | + | carbon source |
31886 | 17748 | thymidine | + | carbon source |
31886 | 53423 | tween 40 | + | carbon source |
31886 | 53426 | tween 80 | + | carbon source |
31886 | 16704 | uridine | + | carbon source |
31886 | 31011 | valerate | + | carbon source |
31886 | 17632 | nitrate | + | reduction |
117125 | 17632 | nitrate | + | reduction |
117125 | 16301 | nitrite | - | reduction |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
31886 | 16136 | hydrogen sulfide | yes |
117125 | 35581 | indole | no |
enzymes
@ref | value | activity | ec |
---|---|---|---|
31886 | alkaline phosphatase | + | 3.1.3.1 |
31886 | catalase | + | 1.11.1.6 |
31886 | gelatinase | + | |
31886 | cytochrome oxidase | + | 1.9.3.1 |
31886 | urease | + | 3.5.1.5 |
117125 | oxidase | + | |
117125 | catalase | + | 1.11.1.6 |
117125 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | - | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | + | 3.4.21.4 |
68382 | alpha-chymotrypsin | + | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
117125 | - | + | + | - | - | + | - | - | + | + | + | + | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | sampling date | isolation date |
---|---|---|---|---|---|---|---|
7526 | marine sediment | Atlantic Ocean, near Halifax harbour, Emerald Basin | Canada | CAN | North America | ||
60227 | Marine sediment | near Halifax,Atlantic Ocean | Canada | CAN | North America | 2003-06-01 | |
117125 | Environment, Cold marine sediment, military waste dumping site | Emerald basin, Atlantic Ocean, Halifax | Canada | CAN | North America | 2003 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Marine |
#Environmental | #Aquatic | #Sediment |
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
7526 | 1 | Risk group (German classification) |
117125 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 7526
- description: Shewanella canadensis strain HAW-EB2 16S ribosomal RNA gene, partial sequence
- accession: AY579749
- length: 1285
- database: ena
- NCBI tax ID: 271096
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Shewanella canadensis HAW-EB2 | GCA_003966225 | contig | ncbi | 271096 |
66792 | Shewanella canadensis HAW-EB2 | 2902540964 | draft | img | 271096 |
GC content
- @ref: 31886
- GC-content: 46.4
External links
@ref: 7526
culture collection no.: DSM 18399, CCUG 54553, NCIMB 14238, CIP 109520
straininfo link
- @ref: 83249
- straininfo: 309558
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 17766891 | Shewanella canadensis sp. nov. and Shewanella atlantica sp. nov., manganese dioxide- and hexahydro-1,3,5-trinitro-1,3,5-triazine-reducing, psychrophilic marine bacteria. | Zhao JS, Manno D, Thiboutot S, Ampleman G, Hawari J | Int J Syst Evol Microbiol | 10.1099/ijs.0.64596-0 | 2007 | Atlantic Ocean, Bacterial Typing Techniques, Canada, Carbohydrate Metabolism, Carbon/metabolism, Carboxylic Acids/metabolism, Cell Membrane/chemistry, DNA Gyrase/genetics, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Genes, rRNA, Geologic Sediments/microbiology, Manganese Compounds/*metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Oxidation-Reduction, Oxides/*metabolism, Phylogeny, Quinones/analysis, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Shewanella/chemistry/*classification/isolation & purification/*metabolism, Triazines/*metabolism, beta-Galactosidase/biosynthesis | Metabolism |
Phylogeny | 24617134 | Isolation and characterization of a novel bacterial strain Shewanella marinisediminis sp. nov. from deep-sea sediments. | Kim YS, Park JS, Yang HO, Kwon HC | J Environ Biol | 2013 | Genes, Bacterial, Geologic Sediments/*microbiology, Marine Biology, Microscopy, Electron, Scanning, Phylogeny, RNA, Ribosomal, 16S/genetics, Shewanella/classification/genetics/*isolation & purification, *Water Microbiology | Enzymology | |
Phylogeny | 34904941 | Shewanella nanhaiensis sp. nov., a marine bacterium isolated from sediment of South China Sea, and emended descriptions of Shewanella woodyi, Shewanella hanedai and Shewanella canadensis. | Cao WR, Li X, Sun YY, Jiang MY, Xu XD, Li YJ | Int J Syst Evol Microbiol | 10.1099/ijsem.0.005152 | 2021 | Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, *Shewanella/classification/isolation & purification, Vitamin K 2/chemistry | Transcriptome |
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
7526 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18399) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-18399 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
31886 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28146 | 28776041 | |
33305 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/7273 | ||||
60227 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 54553) | https://www.ccug.se/strain?id=54553 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
68382 | Automatically annotated from API zym | |||||
83249 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID309558.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
117125 | Curators of the CIP | Collection of Institut Pasteur (CIP 109520) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109520 |