Strain identifier
BacDive ID: 140760
Type strain:
Species: Flavobacterium ardleyense
Strain Designation: A2-1
Strain history: <- Mingyang Zhou, Qilu Univ. of Technology
NCBI tax ID(s): 2038737 (species)
General
@ref: 43538
BacDive-ID: 140760
keywords: 16S sequence, Bacteria, aerobe, psychrophilic, Gram-negative, rod-shaped, colony-forming
description: Flavobacterium ardleyense A2-1 is an aerobe, psychrophilic, Gram-negative bacterium that forms circular colonies and was isolated from From soil.
NCBI tax id
- NCBI tax id: 2038737
- Matching level: species
strain history
- @ref: 67771
- history: <- Mingyang Zhou, Qilu Univ. of Technology
doi: 10.13145/bacdive140760.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacteroidota
- domain: Bacteria
- phylum: Bacteroidota
- class: Flavobacteriia
- order: Flavobacteriales
- family: Flavobacteriaceae
- genus: Flavobacterium
- species: Flavobacterium ardleyense
- full scientific name: Flavobacterium ardleyense Zhou et al. 2017
@ref: 43538
domain: Bacteria
phylum: Bacteroidetes
class: Flavobacteriia
order: Flavobacteriales
family: Flavobacteriaceae
genus: Flavobacterium
species: Flavobacterium ardleyense
strain designation: A2-1
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility |
---|---|---|---|---|---|
43538 | negative | 1-5.2 µm | 0.3-0.5 µm | rod-shaped | no |
67771 | rod-shaped | ||||
67771 | negative |
colony morphology
- @ref: 43538
- colony size: 1-2 mm
- colony color: Yellow
- colony shape: circular
- incubation period: 4 days
- medium used: R2A agar
Culture and growth conditions
culture medium
- @ref: 43538
- name: Reasoner's 2A agar (R2A)
- growth: yes
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
43538 | positive | growth | 4-22 | psychrophilic |
43538 | positive | optimum | 10 | psychrophilic |
67771 | positive | growth | 15 | psychrophilic |
culture pH
@ref | ability | type | pH |
---|---|---|---|
43538 | positive | growth | 6-8 |
43538 | positive | optimum | 6.5 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
43538 | aerobe |
67771 | aerobe |
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
43538 | NaCl | positive | growth | 0-1.5 % |
43538 | NaCl | positive | optimum | 0.5 % |
observation
- @ref: 67771
- observation: quinones: MK-6
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
43538 | 17128 | adipate | - | assimilation |
43538 | 27689 | decanoate | - | assimilation |
43538 | 17634 | D-glucose | - | assimilation |
43538 | 16899 | D-mannitol | - | assimilation |
43538 | 16024 | D-mannose | - | assimilation |
43538 | 30849 | L-arabinose | - | assimilation |
43538 | 25115 | malate | - | assimilation |
43538 | 17306 | maltose | - | assimilation |
43538 | 506227 | N-acetylglucosamine | - | assimilation |
43538 | 18401 | phenylacetate | - | assimilation |
43538 | 32032 | potassium gluconate | - | assimilation |
43538 | 53258 | sodium citrate | - | assimilation |
43538 | 27897 | tryptophan | - | energy source |
43538 | 17234 | glucose | - | fermentation |
43538 | 16991 | dna | - | hydrolysis |
43538 | 28017 | starch | - | hydrolysis |
43538 | 53425 | tween 60 | - | hydrolysis |
43538 | 53426 | tween 80 | - | hydrolysis |
43538 | 17632 | nitrate | - | reduction |
43538 | casein | + | hydrolysis | |
43538 | 4767 | elastin | + | hydrolysis |
43538 | esculin ferric citrate | + | hydrolysis | |
43538 | 5291 | gelatin | + | hydrolysis |
43538 | 30089 | acetate | + | oxidation |
43538 | 13705 | acetoacetate | + | oxidation |
43538 | 16947 | citrate | + | oxidation |
43538 | 14314 | D-glucose 6-phosphate | + | oxidation |
43538 | 23652 | dextrin | + | oxidation |
43538 | 16537 | galactarate | + | oxidation |
43538 | 5291 | gelatin | + | oxidation |
43538 | 70744 | glycine-proline | + | oxidation |
43538 | 29991 | L-aspartate | + | oxidation |
43538 | 29985 | L-glutamate | + | oxidation |
43538 | 53423 | tween 40 | + | oxidation |
antibiotic resistance
@ref | ChEBI | metabolite | is antibiotic | is sensitive | sensitivity conc. |
---|---|---|---|---|---|
43538 | 28077 | rifampicin | yes | yes | 5 µg (disc) |
43538 | 28971 | ampicillin | yes | yes | 10 µg (disc) |
43538 | 7507 | neomycin | yes | yes | 30 µg (disc) |
43538 | 17698 | chloramphenicol | yes | yes | 30 µg (disc) |
43538 | 204928 | cefotaxime | yes | yes | 30 µg (disc) |
43538 | 71415 | nitrofurantoin | yes | yes | 300 µg (disc) |
43538 | 3393 | carbenicillin | yes | yes | 100 µg (disc) |
43538 | 8309 | polymyxin b | yes | yes | 300 Unit (disc) |
metabolite production
- @ref: 43538
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 43538
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
43538 | catalase | + | 1.11.1.6 |
43538 | cytochrome oxidase | + | 1.9.3.1 |
43538 | alkaline phosphatase | + | 3.1.3.1 |
43538 | esterase (C 4) | + | |
43538 | esterase Lipase (C 8) | + | |
43538 | leucine arylamidase | + | 3.4.11.1 |
43538 | valine arylamidase | + | |
43538 | acid phosphatase | + | 3.1.3.2 |
43538 | naphthol-AS-BI-phosphohydrolase | + | |
43538 | lipase (C 14) | - | |
43538 | cystine arylamidase | - | 3.4.11.3 |
43538 | trypsin | - | 3.4.21.4 |
43538 | alpha-chymotrypsin | - | 3.4.21.1 |
43538 | alpha-galactosidase | - | 3.2.1.22 |
43538 | beta-glucuronidase | - | 3.2.1.31 |
43538 | alpha-glucosidase | - | 3.2.1.20 |
43538 | beta-glucosidase | - | 3.2.1.21 |
43538 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
43538 | alpha-mannosidase | - | 3.2.1.24 |
43538 | alpha-fucosidase | - | 3.2.1.51 |
43538 | tryptophan deaminase | - | 4.1.99.1 |
43538 | urease | - | 3.5.1.5 |
43538 | beta-galactosidase | - | 3.2.1.23 |
43538 | arginine dihydrolase | - | 3.5.3.6 |
fatty acid profile
fatty acids
@ref fatty acid percentage 43538 C14:0 0.8 43538 C14:1ω5c 0.5 43538 C15:0 1.5 43538 C16:0 38.7 43538 C16:1ω7c / C16:1ω6c 42.7 43538 C17:0 0.6 43538 C18:1ω7c / C18:1ω6c 10.2 43538 11-methyl C18:1ω7c 1.1 43538 C19:0cycloω10c / C19:1ω6c 0.5 43538 C8:0 3OH 1.3 - type of FA analysis: whole cell analysis
- incubation medium: R2A broth
- agar/liquid: liquid
- incubation temperature: 10
- incubation time: 4
- incubation_oxygen: aerobic
- software version: Sherlock 4.5
- library/peak naming table: TSBA40
- system: MIS MIDI
- cutoff value:
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | latitude | longitude |
---|---|---|---|---|---|---|---|
67771 | From soil | Ardley Island, South Shetland Islands, Antarctica | Antarctica | ATA | Australia and Oceania | ||
43538 | soil sample collected on Ardley Island | South Shetland Islands | Antarctica | ATA | Australia and Oceania | -62.21 | -58.925 |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
taxonmaps
- @ref: 69479
- File name: preview.99_60524.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_66;96_644;97_12230;98_15044;99_60524&stattab=map
- Last taxonomy: Flavobacterium ardleyense subclade
- 16S sequence: KX911209
- Sequence Identity:
- Total samples: 294
- soil counts: 20
- aquatic counts: 251
- animal counts: 20
- plant counts: 3
Sequence information
16S sequences
- @ref: 43538
- description: 16S rRNA gene sequence
- accession: KX911209
- database: nuccore
GC content
@ref | GC-content | method |
---|---|---|
43538 | 34 | thermal denaturation, midpoint method (Tm) |
67771 | 34.0 |
External links
@ref: 43538
culture collection no.: CCTCC AB 2017157, KCTC 52644
literature
- topic: Phylogeny
- Pubmed-ID: 28893368
- title: Flavobacterium ardleyense sp. nov., isolated from Antarctic soil.
- authors: Zhou MY, Zhang XY, Yang XD, Zhang YJ, He HL, Ning D
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.002241
- year: 2017
- mesh: Antarctic Regions, Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacterium/*classification/genetics/isolation & purification, Phosphatidylethanolamines/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry
- topic2: Transcriptome
Reference
@id | authors | title | doi/url | journal | pubmed |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
43538 | Ming-Yang Zhou, Xi-Ying Zhang, Xiao-Deng Yang, Yan-Jiao Zhang, Hai-Lun He, Daling Ning | Flavobacterium ardleyense sp. nov., isolated from Antarctic soil | 10.1099/ijsem.0.002241 | IJSEM 67: 3996-4001 2017 | 28893368 |
67771 | Curators of the KCTC | https://kctc.kribb.re.kr/En/Kctc | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ |