Strain identifier

BacDive ID: 140747

Type strain: Yes

Species: Anaerotignum aminivorans

Strain Designation: SH021

Strain history: A. Ueki; Fac. of Agric., Yamagata Univ., Japan; SH021.

NCBI tax ID(s): 433287 (species)

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General

@ref: 64905

BacDive-ID: 140747

DSM-Number: 103575

keywords: 16S sequence, Bacteria, obligate anaerobe, chemoorganotroph, Gram-positive, motile, rod-shaped, colony-forming

description: Anaerotignum aminivorans SH021 is an obligate anaerobe, chemoorganotroph, Gram-positive bacterium that forms circular colonies and was isolated from a methanogenic reactor treating waste collected from cattle farms.

NCBI tax id

  • NCBI tax id: 433287
  • Matching level: species

strain history

@refhistory
64905<- A. Ueki, Yamagata Univ., Tsuruoka, Japan; SH021
67770A. Ueki; Fac. of Agric., Yamagata Univ., Japan; SH021.

doi: 10.13145/bacdive140747.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Lachnospiraceae
  • genus: Anaerotignum
  • species: Anaerotignum aminivorans
  • full scientific name: Anaerotignum aminivorans Ueki et al. 2017

@ref: 64905

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Lachnospiraceae

genus: Anaerotignum

species: Anaerotignum aminivorans

full scientific name: Anaerotignum aminivorans Ueki et al. 2017

strain designation: SH021

type strain: yes

Morphology

cell morphology

  • @ref: 43524
  • gram stain: positive
  • cell length: 2.5-8 µm
  • cell width: 0.7 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: peritrichous

colony morphology

  • @ref: 43524
  • colony size: 0.5-0.7 mm
  • colony color: greyish white
  • colony shape: circular
  • incubation period: 2 days
  • medium used: PY4S agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43524PY4S agaryes
64905PY + X MEDIUM (N2/CO2) (DSMZ Medium 104c)yeshttps://mediadive.dsmz.de/medium/104cName: PY + X MEDIUM (N2/CO2) (DSMZ Medium 104c; with strain-specific modifications) Composition: Yeast extract 10.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l Casamino acids 3.0 g/l Na2CO3 1.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
43524positivegrowth15-40
43524positiveoptimum35mesophilic
64905positivegrowth30mesophilic
67770positivegrowth30mesophilic

culture pH

@refabilitytypepH
43524positivegrowth6.1-8.2
43524positiveoptimum6.1-7.7

Physiology and metabolism

oxygen tolerance

  • @ref: 43524
  • oxygen tolerance: obligate anaerobe

nutrition type

  • @ref: 43524
  • type: chemoorganotroph

halophily

@refsaltgrowthtested relationconcentrationhalophily level
43524NaClpositivegrowth0-20 g/L
43524NaClpositiveoptimum0 g/Lnon-halophilic

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
43524283402-aminobutyrate-assimilation
4352437080acrylate-assimilation
4352427613amygdalin-assimilation
4352422599arabinose-assimilation
4352485146carboxymethylcellulose-assimilation
4352417057cellobiose-assimilation
4352462968cellulose-assimilation
4352435899crotonate-assimilation
4352423652dextrin-assimilation
4352425115malate-assimilation
435244853esculin-assimilation
4352416236ethanol-assimilation
4352428757fructose-assimilation
4352429806fumarate-assimilation
4352428260galactose-assimilation
4352417234glucose-assimilation
4352417754glycerol-assimilation
4352415428glycine-assimilation
4352428087glycogen-assimilation
4352417268myo-inositol-assimilation
4352415443inulin-assimilation
4352416467L-arginine-assimilation
4352417196L-asparagine-assimilation
4352429991L-aspartate-assimilation
4352429985L-glutamate-assimilation
4352418050L-glutamine-assimilation
4352415971L-histidine-assimilation
4352418019L-lysine-assimilation
4352416643L-methionine-assimilation
4352417295L-phenylalanine-assimilation
4352417203L-proline-assimilation
4352416828L-tryptophan-assimilation
4352417716lactose-assimilation
4352417306maltose-assimilation
4352429864mannitol-assimilation
4352437684mannose-assimilation
435246731melezitose-assimilation
4352428053melibiose-assimilation
4352417309pectin-assimilation
4352416634raffinose-assimilation
4352426546rhamnose-assimilation
4352433942ribose-assimilation
4352417814salicin-assimilation
4352430911sorbitol-assimilation
4352428017starch-assimilation
4352430031succinate-assimilation
4352417992sucrose-assimilation
4352427082trehalose-assimilation
4352437166xylan-assimilation
4352418222xylose-assimilation
435245291gelatin-hydrolysis
4352428017starch-hydrolysis
4352417632nitrate-reduction
4352424996lactate+assimilation
4352416977L-alanine+assimilation
4352417115L-serine+assimilation
4352416857L-threonine+assimilation
4352415361pyruvate+assimilation
435244853esculin+hydrolysis
43524casamino acids+/-assimilation
4352417191L-isoleucine+/-assimilation
4352415603L-leucine+/-assimilation
4352416414L-valine+/-assimilation

metabolite production

@refChebi-IDmetaboliteproduction
4352435581indoleno
4352430089acetateyes
4352417968butyrateyes
4352416136hydrogen sulfideyes
4352448944isobutyrateyes
4352448942isovalerateyes
4352417272propionateyes

enzymes

@refvalueactivityec
43524cytochrome oxidase-1.9.3.1
43524catalase-1.11.1.6
43524lecithinase-
43524lipase (C 14)-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
43524a methanogenic reactor treating waste collected from cattle farmsBetsukai-machi, HokkaidoJapanJPNAsia
64905methanogenic reactor treating waste from cattlefarmsHokkaidoJapanJPNAsia
67770Methanogenic reactor treating waste from cattle farmsJapanJPNAsia

isolation source categories

Cat1Cat2Cat3
#Condition#Anoxic (anaerobic)
#Engineered#Bioreactor
#Host#Mammals#Bovinae (Cow, Cattle)
#Host Body Product#Gastrointestinal tract#Feces (Stool)

taxonmaps

  • @ref: 69479
  • File name: preview.99_114291.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_41;96_13880;97_16910;98_77372;99_114291&stattab=map
  • Last taxonomy: Anaerotignum aminivorans subclade
  • 16S sequence: AB298756
  • Sequence Identity:
  • Total samples: 597
  • soil counts: 16
  • aquatic counts: 71
  • animal counts: 477
  • plant counts: 33

Safety information

risk assessment

  • @ref: 64905
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 64905
  • description: Anaerotignum aminivorans gene for 16S rRNA, partial sequence
  • accession: AB298756
  • length: 1471
  • database: ena
  • NCBI tax ID: 433287

GC content

  • @ref: 64905
  • GC-content: 38.2
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 64905

culture collection no.: JCM 31556, DSM 103575, KCTC 15705

straininfo link

  • @ref: 96784
  • straininfo: 406740

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny28905695Description of Anaerotignum aminivorans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor, and reclassification of Clostridium propionicum, Clostridium neopropionicum and Clostridium lactatifermentans as species of the genus Anaerotignum.Ueki A, Goto K, Ohtaki Y, Kaku N, Ueki KInt J Syst Evol Microbiol10.1099/ijsem.0.0022682017Animals, Bacterial Typing Techniques, Base Composition, Bioreactors/microbiology, Cattle, Clostridiales/*classification/genetics/isolation & purification, Clostridium/*classification, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Japan, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Waste Disposal, FluidTranscriptome
Phylogeny31680219Anaerotignum faecicola sp. nov., isolated from human faeces.Choi SH, Kim JS, Park JE, Lee KC, Eom MK, Oh BS, Yu SY, Kang SW, Han KI, Suh MK, Lee DH, Yoon H, Kim BY, Lee JH, Lee JH, Lee JS, Park SHJ Microbiol10.1007/s12275-019-9268-32019Bacterial Typing Techniques, Base Composition, Clostridiales/*classification/genetics/*isolation & purification/physiology, DNA, Bacterial/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Feces/*microbiology, Humans, Hydrogen-Ion Concentration, *Microbiota, Peptidoglycan/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seoul, Sequence Analysis, DNA, TemperaturePathogenicity

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43524Atsuko Ueki, Kazushi Goto, Yoshimi Ohtaki, Nobuo Kaku, Katsuji UekiDescription of Anaerotignum aminivorans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor, and reclassification of Clostridium propionicum, Clostridium neopropionicum and Clostridium lactatifermentans as species of the genus Anaerotignum10.1099/ijsem.0.002268IJSEM 67: 4146-4153 201728905695
64905Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-103575Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 103575)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
96784Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID406740.1