Strain identifier

BacDive ID: 140689

Type strain: Yes

Species: Laceyella thermophila

Strain history: W.-J. Li; Yunnan Inst. of Microbiol., Yunnan Univ., China; YIM 79486.

NCBI tax ID(s): 1960874 (species)

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General

@ref: 66517

BacDive-ID: 140689

DSM-Number: 46882

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive

description: Laceyella thermophila CCTC AB 2015040 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from sediment sample collected from Jinze hot spring.

NCBI tax id

  • NCBI tax id: 1960874
  • Matching level: species

strain history

@refhistory
66517<- W.-J. Li, Yunnan Univ., Yunnan Inst. Microbiol. (YIM), Kunming, China; YIM 79486 <- H. Ming
67770W.-J. Li; Yunnan Inst. of Microbiol., Yunnan Univ., China; YIM 79486.

doi: 10.13145/bacdive140689.20241212.9.2

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Thermoactinomycetaceae
  • genus: Laceyella
  • species: Laceyella thermophila
  • full scientific name: Laceyella thermophila Ming et al. 2017

@ref: 66517

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Thermoactinomycetaceae

genus: Laceyella

species: Laceyella sp.

full scientific name: Laceyella sp.

type strain: yes

Morphology

cell morphology

  • @ref: 43458
  • gram stain: positive

colony morphology

  • @ref: 43458
  • incubation period: 7 days
  • medium used: T5 agar

multimedia

  • @ref: 66517
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_46882.jpg
  • caption: Medium 65 50°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43458T5 agaryes
66517GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
66517NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperature
43458positivegrowth37-65
43458positiveoptimum50
66517positivegrowth50
67770positivegrowth45

culture pH

@refabilitytypepH
43458positivegrowth6-8
43458positiveoptimum7-7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 43458
  • oxygen tolerance: aerobe

spore formation

  • @ref: 43458
  • spore description: terminally located, produced on short sporophores
  • type of spore: endospore
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentrationhalophily level
43458NaClpositivegrowth0-0.5 %(w/v)
43458NaClpositiveoptimum0 %(w/v)non-halophilic

observation

  • @ref: 67770
  • observation: quinones: MK-9, MK-8

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
43458645522-hydroxybutyrate-carbon source
43458167632-oxobutanoate-carbon source
43458739183-O-methyl-D-glucose-carbon source
43458181014-hydroxyphenylacetic acid-carbon source
4345830089acetate-carbon source
4345873706bromosuccinate-carbon source
4345817057cellobiose-carbon source
4345816947citrate-carbon source
4345818333D-arabitol-carbon source
434588391D-gluconate-carbon source
4345814314D-glucose 6-phosphate-carbon source
4345816899D-mannitol-carbon source
4345833801D-saccharate-carbon source
4345823652dextrin-carbon source
43458370543-hydroxybutyrate-carbon source
4345815740formate-carbon source
4345816537galactarate-carbon source
4345816865gamma-aminobutyric acid-carbon source
4345817754glycerol-carbon source
4345817596inosine-carbon source
4345824996lactate-carbon source
4345817716lactose-carbon source
4345825115malate-carbon source
4345874611methyl (R)-lactate-carbon source
4345837657methyl D-glucoside-carbon source
4345817268myo-inositol-carbon source
4345817272propionate-carbon source
4345826490quinate-carbon source
4345816634raffinose-carbon source
4345817814salicin-carbon source
4345817164stachyose-carbon source
4345817992sucrose-carbon source
4345827082trehalose-carbon source
43458casein-degradation
4345862968cellulose-degradation
4345853424tween 20-degradation
4345853423tween 40-degradation
4345853425tween 60-degradation
4345853426tween 80-degradation
4345837166xylan-degradation
4345816523D-serine-nitrogen source
4345815428glycine-nitrogen source
4345816283L-cystine-nitrogen source
4345829985L-glutamate-nitrogen source
4345815971L-histidine-nitrogen source
4345817191L-isoleucine-nitrogen source
4345818019L-lysine-nitrogen source
4345816643L-methionine-nitrogen source
4345817295L-phenylalanine-nitrogen source
4345818183L-pyroglutamic acid-nitrogen source
4345817115L-serine-nitrogen source
4345816857L-threonine-nitrogen source
4345817895L-tyrosine-nitrogen source
4345863154N-acetyl-beta-D-mannosamine-nitrogen source
4345828037N-acetylgalactosamine-nitrogen source
43458506227N-acetylglucosamine-nitrogen source
4345835418n-acetylneuraminate-nitrogen source
4345817632nitrate-reduction
43458309162-oxoglutarate+carbon source
4345813705acetoacetate+carbon source
4345815824D-fructose+carbon source
4345878697D-fructose 6-phosphate+carbon source
4345828847D-fucose+carbon source
4345812936D-galactose+carbon source
4345818024D-galacturonic acid+carbon source
4345817634D-glucose+carbon source
4345815748D-glucuronate+carbon source
4345816024D-mannose+carbon source
4345817924D-sorbitol+carbon source
4345832528turanose+carbon source
4345828066gentiobiose+carbon source
4345818287L-fucose+carbon source
4345817464L-galactonic acid gamma-lactone+carbon source
4345862345L-rhamnose+carbon source
4345817306maltose+carbon source
4345828053melibiose+carbon source
4345815361pyruvate+carbon source
434584853esculin+hydrolysis
434585291gelatin+hydrolysis
4345817309pectin+hydrolysis
4345816199urea+hydrolysis
4345832323glucuronamide+nitrogen source
4345870744glycine-proline+nitrogen source
4345816977L-alanine+nitrogen source
4345816467L-arginine+nitrogen source
4345817196L-asparagine+nitrogen source
4345817203L-proline+nitrogen source
4345890608L-valine 2-naphthylamide+nitrogen source

metabolite production

  • @ref: 43458
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: no

enzymes

@refvalueactivityec
43458urease+3.5.1.5
43458alkaline phosphatase+3.1.3.1
43458esterase (C 4)+
43458naphthol-AS-BI-phosphohydrolase+
43458esterase Lipase (C 8)-
43458lipase (C 14)-
43458leucine arylamidase-3.4.11.1
43458valine arylamidase-
43458cystine arylamidase-3.4.11.3
43458trypsin-3.4.21.4
43458alpha-chymotrypsin-3.4.21.1
43458acid phosphatase-3.1.3.2
43458alpha-galactosidase-3.2.1.22
43458beta-galactosidase-3.2.1.23
43458beta-glucuronidase-3.2.1.31
43458alpha-glucosidase-3.2.1.20
43458beta-glucosidase-3.2.1.21
43458N-acetyl-beta-glucosaminidase-3.2.1.52
43458beta-D-fucosidase-3.2.1.38
43458alpha-mannosidase-3.2.1.24

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43458C15:0 anteiso23.2
    43458C17:0 anteiso13.4
    43458C14:01
    43458C16:05.7
    43458C18:03.4
    43458C18:1ω7c / C18:1ω6c2.3
    43458C14:0 iso0.8
    43458C15:0 iso26.4
    43458C15:0 iso 3OH2
    43458C16:0 iso5.1
    43458C17:0 iso15.9
  • type of FA analysis: whole cell analysis
  • incubation medium: TSB
  • agar/liquid: liquid
  • incubation temperature: 50
  • incubation time: 3
  • incubation_oxygen: aerobic
  • software version: Sherlock 6.1
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • method/protocol: Minnikin, 1980
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
43458sediment sample collected from Jinze hot springTengchong county, Yunnan provinceChinaCHNAsia25.43398.45
67770Sediment from Jinze hot spring in Tengchong CountyYunnan Province, south-west ChinaChinaCHNAsia
66517hot spring sedimentChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Condition#Thermophilic (>45°C)
#Environmental#Aquatic#Sediment
#Environmental#Aquatic#Thermal spring

taxonmaps

  • @ref: 69479
  • File name: preview.99_180895.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_284;96_5917;97_7121;98_61556;99_180895&stattab=map
  • Last taxonomy: Laceyella thermophila
  • 16S sequence: KP232927
  • Sequence Identity:
  • Total samples: 330
  • soil counts: 89
  • aquatic counts: 52
  • animal counts: 156
  • plant counts: 33

Sequence information

16S sequences

  • @ref: 43458
  • description: Laceyella thermophila strain YIM 79486 16S ribosomal RNA gene, partial sequence
  • accession: KP232927
  • database: nuccore

Genome sequences

  • @ref: 66792
  • description: Laceyella thermophila YIM 79486
  • accession: 2784132171
  • assembly level: draft
  • database: img
  • NCBI tax ID: 1644115

GC content

  • @ref: 67770
  • GC-content: 47.8
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 66517

culture collection no.: CCTC AB 2015040, NBRC 110772, YIM 79486, DSM 46882, JCM 30657, BCRC 16987, CCTCC AB 2015040

straininfo link

  • @ref: 96777
  • straininfo: 403481

literature

  • topic: Phylogeny
  • Pubmed-ID: 28820123
  • title: Laceyella thermophila sp. nov., a thermophilic bacterium isolated from a hot spring.
  • authors: Ming H, Ji WL, Li S, Zhao ZL, Zhang LY, Meng XL, Zhou EM, Nie GX, Li WJ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002057
  • year: 2017
  • mesh: Bacillales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Hot Springs/*microbiology, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43458Hong Ming, Wei-Li Ji, Shuai Li, Zhuo-Li Zhao, Ling-Yu Zhang, Xiao-Lin Meng, En-Min Zhou, Guo-Xing Nie, Wen-Jun LiLaceyella thermophila sp. nov., a thermophilic bacterium isolated from a hot spring10.1099/ijsem.0.002057IJSEM 67: 2953-2958 201728820123
66517Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-46882Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 46882)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
96777Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID403481.1