Strain identifier
BacDive ID: 140678
Type strain:
Species: Zunongwangia endophytica
Strain Designation: CPA58
Strain history: Fidalgo, C. University of Aveiro
NCBI tax ID(s): 1808945 (species)
General
@ref: 43442
BacDive-ID: 140678
keywords: 16S sequence, Bacteria, microaerophile, Gram-negative, rod-shaped
description: Zunongwangia endophytica CPA58 is a microaerophile, Gram-negative, rod-shaped bacterium that was isolated from surface-sterilized aboveground tissues of the halophyte Halimione portulacoides, collected from a salt marsh in Ria de Aveiro.
NCBI tax id
- NCBI tax id: 1808945
- Matching level: species
strain history
- @ref: 67772
- history: Fidalgo, C. University of Aveiro
doi: 10.13145/bacdive140678.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacteroidota
- domain: Bacteria
- phylum: Bacteroidota
- class: Flavobacteriia
- order: Flavobacteriales
- family: Flavobacteriaceae
- genus: Zunongwangia
- species: Zunongwangia endophytica
- full scientific name: Zunongwangia endophytica Fidalgo et al. 2017
@ref: 43442
domain: Bacteria
phylum: Bacteroidetes
class: Flavobacteriia
order: Flavobacteriales
family: Flavobacteriaceae
genus: Zunongwangia
species: Zunongwangia endophytica
strain designation: CPA58
type strain: yes
Morphology
cell morphology
- @ref: 43442
- gram stain: negative
- cell length: 1.38-3.4 µm
- cell width: 0.55-1.13 µm
- cell shape: rod-shaped
- motility: no
colony morphology
- @ref: 43442
- colony size: 0.6-2.2 mm
- colony color: creamy-yellow
- incubation period: 2 days
- medium used: Marine agar
pigmentation
- @ref: 43442
- production: no
- name: Flexirubin-type
Culture and growth conditions
culture medium
- @ref: 43442
- name: Marine agar (MA)
- growth: yes
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
43442 | positive | growth | 4-30 | |
43442 | positive | optimum | 26 | mesophilic |
67772 | positive | minimum | 4 | psychrophilic |
67772 | positive | optimum | 26 | mesophilic |
67772 | positive | maximum | 30 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
43442 | positive | growth | 6-9 | alkaliphile |
43442 | positive | optimum | 6-8 | |
67772 | positive | optimum | 6.0-8.0 | |
67772 | positive | maximum | 9.0 | |
67772 | positive | minimum | 6.0 |
Physiology and metabolism
oxygen tolerance
- @ref: 43442
- oxygen tolerance: microaerophile
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
43442 | NaCl | positive | growth | 2-10 %(w/v) |
43442 | NaCl | positive | optimum | 2-3 %(w/v) |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
43442 | 17128 | adipate | - | assimilation |
43442 | 27689 | decanoate | - | assimilation |
43442 | 16947 | citrate | - | assimilation |
43442 | 17634 | D-glucose | - | assimilation |
43442 | 16899 | D-mannitol | - | assimilation |
43442 | 16024 | D-mannose | - | assimilation |
43442 | 30849 | L-arabinose | - | assimilation |
43442 | 25115 | malate | - | assimilation |
43442 | 17306 | maltose | - | assimilation |
43442 | 506227 | N-acetylglucosamine | - | assimilation |
43442 | 18401 | phenylacetate | - | assimilation |
43442 | 32032 | potassium gluconate | - | assimilation |
43442 | 53258 | sodium citrate | - | assimilation |
43442 | 27613 | amygdalin | - | builds acid from |
43442 | 18305 | arbutin | - | builds acid from |
43442 | 15963 | ribitol | - | builds acid from |
43442 | 17108 | D-arabinose | - | builds acid from |
43442 | 18333 | D-arabitol | - | builds acid from |
43442 | 17057 | cellobiose | - | builds acid from |
43442 | 15824 | D-fructose | - | builds acid from |
43442 | 28847 | D-fucose | - | builds acid from |
43442 | 12936 | D-galactose | - | builds acid from |
43442 | 17634 | D-glucose | - | builds acid from |
43442 | 62318 | D-lyxose | - | builds acid from |
43442 | 16899 | D-mannitol | - | builds acid from |
43442 | 16024 | D-mannose | - | builds acid from |
43442 | 16988 | D-ribose | - | builds acid from |
43442 | 17924 | D-sorbitol | - | builds acid from |
43442 | 16443 | D-tagatose | - | builds acid from |
43442 | 65327 | D-xylose | - | builds acid from |
43442 | 16813 | galactitol | - | builds acid from |
43442 | 17113 | erythritol | - | builds acid from |
43442 | 28066 | gentiobiose | - | builds acid from |
43442 | 17754 | glycerol | - | builds acid from |
43442 | 28087 | glycogen | - | builds acid from |
43442 | 17268 | myo-inositol | - | builds acid from |
43442 | 15443 | inulin | - | builds acid from |
43442 | 30849 | L-arabinose | - | builds acid from |
43442 | 18403 | L-arabitol | - | builds acid from |
43442 | 18287 | L-fucose | - | builds acid from |
43442 | 62345 | L-rhamnose | - | builds acid from |
43442 | 17266 | L-sorbose | - | builds acid from |
43442 | 65328 | L-xylose | - | builds acid from |
43442 | 17716 | lactose | - | builds acid from |
43442 | 17306 | maltose | - | builds acid from |
43442 | 6731 | melezitose | - | builds acid from |
43442 | 28053 | melibiose | - | builds acid from |
43442 | 43943 | methyl alpha-D-mannoside | - | builds acid from |
43442 | 74863 | methyl beta-D-xylopyranoside | - | builds acid from |
43442 | 37657 | methyl D-glucoside | - | builds acid from |
43442 | 506227 | N-acetylglucosamine | - | builds acid from |
43442 | potassium 2-dehydro-D-gluconate | - | builds acid from | |
43442 | potassium 5-dehydro-D-gluconate | - | builds acid from | |
43442 | 32032 | potassium gluconate | - | builds acid from |
43442 | 16634 | raffinose | - | builds acid from |
43442 | 17814 | salicin | - | builds acid from |
43442 | 28017 | starch | - | builds acid from |
43442 | 17992 | sucrose | - | builds acid from |
43442 | 27082 | trehalose | - | builds acid from |
43442 | 32528 | turanose | - | builds acid from |
43442 | 17151 | xylitol | - | builds acid from |
43442 | 17634 | D-glucose | - | fermentation |
43442 | 85146 | carboxymethylcellulose | - | hydrolysis |
43442 | casein | - | hydrolysis | |
43442 | 16991 | dna | - | hydrolysis |
43442 | 28017 | starch | - | hydrolysis |
43442 | 17632 | nitrate | - | reduction |
43442 | esculin ferric citrate | + | builds acid from | |
43442 | 4853 | esculin | + | hydrolysis |
43442 | 5291 | gelatin | + | hydrolysis |
43442 | O-nitrophenyl-beta-D-galactopyranosid | + | hydrolysis | |
43442 | 53424 | tween 20 | + | hydrolysis |
43442 | 37166 | xylan | + | hydrolysis |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
43442 | 16136 | hydrogen sulfide | no |
43442 | 35581 | indole | no |
enzymes
@ref | value | activity | ec |
---|---|---|---|
43442 | alkaline phosphatase | + | 3.1.3.1 |
43442 | esterase (C 4) | + | |
43442 | esterase Lipase (C 8) | + | |
43442 | leucine arylamidase | + | 3.4.11.1 |
43442 | valine arylamidase | + | |
43442 | trypsin | + | 3.4.21.4 |
43442 | acid phosphatase | + | 3.1.3.2 |
43442 | naphthol-AS-BI-phosphohydrolase | + | |
43442 | alpha-glucosidase | + | 3.2.1.20 |
43442 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
43442 | cystine arylamidase | +/- | 3.4.11.3 |
43442 | alpha-chymotrypsin | +/- | 3.4.21.1 |
43442 | alpha-galactosidase | +/- | 3.2.1.22 |
43442 | beta-galactosidase | +/- | 3.2.1.23 |
43442 | lipase (C 14) | - | |
43442 | beta-glucuronidase | - | 3.2.1.31 |
43442 | alpha-mannosidase | - | 3.2.1.24 |
43442 | alpha-fucosidase | - | 3.2.1.51 |
43442 | catalase | + | 1.11.1.6 |
43442 | cytochrome oxidase | - | 1.9.3.1 |
43442 | beta-glucosidase | + | 3.2.1.21 |
43442 | protease | + | |
43442 | beta-galactosidase | + | 3.2.1.23 |
43442 | arginine dihydrolase | - | 3.5.3.6 |
43442 | urease | - | 3.5.1.5 |
fatty acid profile
fatty acids
@ref fatty acid percentage ECL 43442 C15:0 anteiso 8.4 43442 C17:1 anteiso ω9c 1.4 43442 C15:0 6.6 43442 C15:0 2OH 2 43442 C15:1ω6c 1.4 43442 C16:17c / iso-C15:02-OH 13.5 43442 C17:0 2OH 1.6 43442 C17:1ω6c 1.9 43442 C15:0 iso 20.8 43442 C15:0 iso 3OH 2.8 43442 C15:1 iso G 7.4 43442 C16:0 iso 1.3 43442 C16:0 iso 3OH 1.4 43442 C17:0 iso 3OH 11.7 43442 iso-C17:9c / 10-methyl-C16:0 3.8 43442 Unknown ECL 13.563 7.6 13563 43442 Unknown ECL 13.652 1 43442 Unknown ECL 15.938 1.2 - type of FA analysis: whole cell analysis
- incubation medium: Marine agar
- agar/liquid: agar
- incubation temperature: 26
- incubation time: 2
- incubation_oxygen: microaerophil
- software version: Sherlock 6.0
- library/peak naming table: TSBA6
- system: MIS MIDI
- cutoff value:
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | sampling date | latitude | longitude |
---|---|---|---|---|---|---|---|---|
43442 | surface-sterilized aboveground tissues of the halophyte Halimione portulacoides, collected from a salt marsh in Ria de Aveiro | Ria de Aveiro | Portugal | PRT | Europe | |||
67772 | Surface-sterilized aboveground tissues of the halophyte Halimione portulacoides, collected from a salt marsh | Aveiro, Aveiro | Portugal | PRT | Europe | 2012 | 40.6582 | -8.68905 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Terrestrial | #Salt marsh |
#Host | #Plants | #Shrub (Scrub) |
#Host Body-Site | #Plant | #Sterilized plant part |
taxonmaps
- @ref: 69479
- File name: preview.99_73979.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_52;96_1827;97_39782;98_51769;99_73979&stattab=map
- Last taxonomy: Zunongwangia endophytica subclade
- 16S sequence: KY310594
- Sequence Identity:
- Total samples: 69
- soil counts: 4
- aquatic counts: 56
- animal counts: 2
- plant counts: 7
Safety information
risk assessment
- @ref: 67772
- biosafety level: 1
Sequence information
16S sequences
- @ref: 43442
- description: 16S rRNA gene sequence
- accession: KY310594
- database: nuccore
GC content
- @ref: 43442
- GC-content: 35.1
- method: high performance liquid chromatography (HPLC)
External links
@ref: 43442
culture collection no.: CECT 9128, LMG 29517, UCCCB46
literature
- topic: Phylogeny
- Pubmed-ID: 28853694
- title: Zunongwangia endophytica sp. nov., an endophyte isolated from the salt marsh plant, Halimione portulacoides, and emended description of the genus Zunongwangia.
- authors: Fidalgo C, Martins R, Proenca DN, Morais PV, Alves A, Henriques I
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.002069
- year: 2017
- mesh: Amaranthaceae/*microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Endophytes/classification/genetics/isolation & purification, Fatty Acids/chemistry, Flavobacteriaceae/*classification/genetics/isolation & purification, *Phylogeny, Portugal, RNA, Ribosomal, 16S/genetics, Salt-Tolerant Plants/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry, *Wetlands
- topic2: Enzymology
Reference
@id | authors | title | doi/url | journal | pubmed |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
43442 | Catia Fidalgo, Ricardo Martins, Diogo Neves Proença, Paula V. Morais, Artur Alves, Isabel Henriques | Zunongwangia endophytica sp. nov., an endophyte isolated from the salt marsh plant, Halimione portulacoides, and emended description of the genus Zunongwangia | 10.1099/ijsem.0.002069 | IJSEM 67: 3004-3009 2017 | 28853694 |
67772 | Curators of the UCCCB | https://ucccb.uc.pt/ | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ |