Strain identifier

BacDive ID: 140676

Type strain: Yes

Species: Flavobacterium fluminis

Strain Designation: 3R17

Strain history: CIP <- 2019, JCM <- 2014, S.B. Kim, Chungnam National Univ., South Korea: strain 3R17

NCBI tax ID(s): 1433257 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 8.1 (current version)

General

@ref: 43440

BacDive-ID: 140676

keywords: 16S sequence, Bacteria, aerobe, chemoorganotroph, Gram-negative, motile, rod-shaped, colony-forming

description: Flavobacterium fluminis 3R17 is an aerobe, chemoorganotroph, Gram-negative bacterium that forms circular colonies and was isolated from freshwater sample from the surface of the Keum River near Daejeon City.

NCBI tax id

  • NCBI tax id: 1433257
  • Matching level: species

strain history

@refhistory
67770S. B. Kim; Chungnam Natl. Univ., South Korea; 3R17.
67771<- Seung Bum Kim, Chungnam National Univ., Korea
119406CIP <- 2019, JCM <- 2014, S.B. Kim, Chungnam National Univ., South Korea: strain 3R17

doi: 10.13145/bacdive140676.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Flavobacterium
  • species: Flavobacterium fluminis
  • full scientific name: Flavobacterium fluminis Ahn et al. 2017

@ref: 43440

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Flavobacterium

species: Flavobacterium fluminis

strain designation: 3R17

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangement
43440negative1.0-1.5 µm0.4-0.5 µmrod-shapedyesgliding
67771rod-shapedyes
67771negative
119406negativerod-shapedno

colony morphology

  • @ref: 43440
  • colony color: bright-yellow
  • colony shape: circular
  • incubation period: 2 days
  • medium used: R2A agar

pigmentation

  • @ref: 43440
  • production: no
  • name: Flexirubin-type

Culture and growth conditions

culture medium

@refnamegrowthlink
43440BHIyes
43440Nutrient agar (NA)yes
43440PDAyes
43440Reasoner's 2A agar (R2A)yes
43440Trypticase Soy Agar (TSA)yes
119406CIP Medium 566yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=566

culture temp

@refgrowthtypetemperaturerange
43440positivegrowth4-30
43440positiveoptimum25mesophilic
67770positivegrowth30mesophilic
67771positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
43440positivegrowth6-9alkaliphile
43440positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
43440aerobe
67771aerobe

nutrition type

  • @ref: 43440
  • type: chemoorganotroph

halophily

@refsaltgrowthtested relationconcentrationhalophily level
43440NaClpositivegrowth0-3 %
43440NaClpositiveoptimum0 %non-halophilic

observation

@refobservation
67770quinones: MK-6
67771quinones: MK-6

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4344017306maltose-assimilation
43440506227N-acetylglucosamine-assimilation
4344029016arginine-hydrolysis
4344085146carboxymethylcellulose-hydrolysis
43440casein-hydrolysis
4344016991dna-hydrolysis
4344028017starch-hydrolysis
4344053426tween 80-hydrolysis
4344018186tyrosine-hydrolysis
4344016199urea-hydrolysis
4344017634D-glucose+assimilation
4344016024D-mannose+assimilation
4344030849L-arabinose+assimilation
4344030089acetate+carbon source
4344040585alpha-cyclodextrin+carbon source
4344017925alpha-D-glucose+carbon source
4344017057cellobiose+carbon source
4344015824D-fructose+carbon source
4344012936D-galactose+carbon source
4344018024D-galacturonic acid+carbon source
4344016024D-mannose+carbon source
4344023652dextrin+carbon source
4344028066gentiobiose+carbon source
4344017754glycerol+carbon source
4344028087glycogen+carbon source
4344073804glycyl L-aspartic acid+carbon source
4344017196L-asparagine+carbon source
4344029991L-aspartate+carbon source
4344029985L-glutamate+carbon source
4344017115L-serine+carbon source
4344016857L-threonine+carbon source
4344017716lactose+carbon source
434406359lactulose+carbon source
4344017306maltose+carbon source
4344051850methyl pyruvate+carbon source
43440506227N-acetylglucosamine+carbon source
4344073784glycyl-l-glutamate+carbon source
4344030031succinate+carbon source
4344032528turanose+carbon source
4344053423tween 40+carbon source
4344053426tween 80+carbon source
4344016704uridine+carbon source
434404853esculin+hydrolysis
434405291gelatin+hydrolysis
4344017632nitrate+reduction

enzymes

@refvalueactivityec
43440catalase+1.11.1.6
43440cytochrome oxidase+1.9.3.1
434402-nitrophenyl beta-D-galactopyranoside+3.2.1.52
43440alkaline phosphatase+3.1.3.1
43440acid phosphatase+3.1.3.2
43440esterase (C 4)+
43440esterase Lipase (C 8)+
43440leucine arylamidase+3.4.11.1
43440valine arylamidase+
43440cystine arylamidase+3.4.11.3
43440trypsin+3.4.21.4
43440naphthol-AS-BI-phosphohydrolase+
43440beta-galactosidase+3.2.1.23
43440alpha-glucosidase+3.2.1.20
43440alpha-mannosidase+3.2.1.24
43440beta-glucosidase-3.2.1.21
43440N-acetyl-beta-glucosaminidase-3.2.1.52
43440alpha-galactosidase-3.2.1.22
43440alpha-chymotrypsin-3.4.21.1

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    4344010-methyl C16:0 / iso-C17:1ω9c1.9
    43440C15:0 anteiso7.8
    43440C12:0ALDE / ECL 10.9281.4
    43440C14:02.1
    43440C15:0 2OH1.6
    43440C15:1ω6c2
    43440C16:07
    43440C16:0 3OH4.4
    43440C16:1ω7c / C16:1ω6c35.8
    43440C17:1ω6c2
    43440C15:0 iso15.9
    43440C15:0 iso 3OH5.2
    43440C15:1 iso G7.4
    43440C17:0 iso 3OH5.5
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A
  • agar/liquid: agar
  • incubation temperature: 25
  • incubation time: 2
  • incubation pH: 7
  • incubation_oxygen: aerobic
  • software version: Sherlock 6.1
  • library/peak naming table: RTSBA6
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
43440freshwater sample from the surface of the Keum River near Daejeon CityDaejeon CityRepublic of KoreaKORAsia36.784127.676
67770Freshwater from the surface of the Keum River near DaejeonRepublic of KoreaKORAsia
67771From freshwater, the surface of the Keum River near Daejeon CityRepublic of KoreaKORAsia
119406Environment, FreshwaterDaejeon, Keum riverRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#River (Creek)
#Environmental#Aquatic#Surface water

Safety information

risk assessment

  • @ref: 119406
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

  • @ref: 43440
  • description: 16S rRNA gene sequence
  • accession: KF891387
  • database: nuccore

GC content

@refGC-contentmethod
4344035.4Thermal denaturation, fluorometry
6777035.4thermal denaturation, midpoint method (Tm)
6777135.4

External links

@ref: 43440

culture collection no.: KCTC 42062, JCM 30338, CIP 111715

straininfo link

  • @ref: 96776
  • straininfo: 413894

literature

  • topic: Phylogeny
  • Pubmed-ID: 28771125
  • title: Flavobacterium fluminis sp. nov. to accommodate an aerobic, halotolerant and gliding flavobacterium isolated from freshwater.
  • authors: Ahn JH, Kim TW, Kim TS, Joung Y, Kim SB
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002135
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacterium/*classification/genetics/isolation & purification, Fresh Water/*microbiology, Phosphatidylethanolamines/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43440Joong-Hyeon Ahn, Tae Woon Kim, Tae-Su Kim, Yochan Joung, Seung Bum KimFlavobacterium fluminis sp. nov. to accommodate an aerobic, halotolerant and gliding flavobacterium isolated from freshwater10.1099/ijsem.0.002135IJSEM 67: 3117-3121 201728771125
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
96776Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID413894.1
119406Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20111715Collection of Institut Pasteur (CIP 111715)