Strain identifier
BacDive ID: 140673
Type strain:
Species: Nocardioides thalensis
Strain history: <- Ahmed Iftikhar, National Agricultural Research Centre, Pakistan; NCCP-696 <- I. U. Khan, YIM; YIM 73001
NCBI tax ID(s): 1914755 (species)
General
@ref: 64867
BacDive-ID: 140673
DSM-Number: 103833
keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-positive, rod-shaped
description: Nocardioides thalensis CCTCC AB 2016296 is an aerobe, Gram-positive, rod-shaped bacterium that was isolated from Thal Desert.
NCBI tax id
- NCBI tax id: 1914755
- Matching level: species
strain history
- @ref: 64867
- history: <- Ahmed Iftikhar, National Agricultural Research Centre, Pakistan; NCCP-696 <- I. U. Khan, YIM; YIM 73001
doi: 10.13145/bacdive140673.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Propionibacteriales
- family: Nocardioidaceae
- genus: Nocardioides
- species: Nocardioides thalensis
- full scientific name: Nocardioides thalensis Khan et al. 2017
@ref: 64867
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Nocardioidaceae
genus: Nocardioides
species: Nocardioides thalensis
full scientific name: Nocardioides thalensis Khan et al. 2017
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility | confidence |
---|---|---|---|---|---|---|
43437 | positive | 1.5-2.4 µm | 0.3-0.4 µm | rod-shaped | no | |
69480 | positive | 100 |
colony morphology
- @ref: 43437
- colony color: cream-coloured
- incubation period: 3 days
- medium used: R2A agar medium
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
43437 | Reasoner's 2A agar (R2A) | yes | ||
64867 | R2A MEDIUM (DSMZ Medium 830) | yes | https://mediadive.dsmz.de/medium/830 | Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
43437 | positive | growth | 20-42 | |
43437 | positive | optimum | 30-37 | mesophilic |
64867 | positive | growth | 28 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
43437 | positive | growth | 5.5-9 | alkaliphile |
43437 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
- @ref: 43437
- oxygen tolerance: aerobe
spore formation
- @ref: 69480
- spore formation: no
- confidence: 99.923
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
43437 | NaCl | positive | growth | 0-4 %(w/v) |
43437 | NaCl | positive | optimum | 0-2 %(w/v) |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
43437 | 16193 | 3-hydroxybenzoate | - | carbon source |
43437 | 37054 | 3-hydroxybutyrate | - | carbon source |
43437 | 16947 | citrate | - | carbon source |
43437 | 16988 | D-ribose | - | carbon source |
43437 | 17268 | myo-inositol | - | carbon source |
43437 | 16977 | L-alanine | - | carbon source |
43437 | 30849 | L-arabinose | - | carbon source |
43437 | 17203 | L-proline | - | carbon source |
43437 | 28053 | melibiose | - | carbon source |
43437 | 9300 | suberic acid | - | carbon source |
43437 | 62968 | cellulose | - | degradation |
43437 | 28017 | starch | - | degradation |
43437 | 53424 | tween 20 | - | degradation |
43437 | 53423 | tween 40 | - | degradation |
43437 | 53425 | tween 60 | - | degradation |
43437 | 53426 | tween 80 | - | degradation |
43437 | 30916 | 2-oxoglutarate | + | carbon source |
43437 | 73918 | 3-O-methyl-D-glucose | + | carbon source |
43437 | 30089 | acetate | + | carbon source |
43437 | 17925 | alpha-D-glucose | + | carbon source |
43437 | 17665 | alpha-D-glucose 6-phosphate | + | carbon source |
43437 | 17057 | cellobiose | + | carbon source |
43437 | 18333 | D-arabitol | + | carbon source |
43437 | 29990 | D-aspartate | + | carbon source |
43437 | 15824 | D-fructose | + | carbon source |
43437 | 78697 | D-fructose 6-phosphate | + | carbon source |
43437 | 28847 | D-fucose | + | carbon source |
43437 | 12936 | D-galactose | + | carbon source |
43437 | 8391 | D-gluconate | + | carbon source |
43437 | 15748 | D-glucuronate | + | carbon source |
43437 | 16899 | D-mannitol | + | carbon source |
43437 | 16634 | raffinose | + | carbon source |
43437 | 17924 | D-sorbitol | + | carbon source |
43437 | 16551 | D-trehalose | + | carbon source |
43437 | 32528 | turanose | + | carbon source |
43437 | 23652 | dextrin | + | carbon source |
43437 | 15740 | formate | + | carbon source |
43437 | 5291 | gelatin | + | carbon source |
43437 | 17754 | glycerol | + | carbon source |
43437 | 17596 | inosine | + | carbon source |
43437 | 29991 | L-aspartate | + | carbon source |
43437 | 18287 | L-fucose | + | carbon source |
43437 | 29985 | L-glutamate | + | carbon source |
43437 | 15971 | L-histidine | + | carbon source |
43437 | 18183 | L-pyroglutamic acid | + | carbon source |
43437 | 62345 | L-rhamnose | + | carbon source |
43437 | 24996 | lactate | + | carbon source |
43437 | 25115 | malate | + | carbon source |
43437 | 17306 | maltose | + | carbon source |
43437 | 37657 | methyl D-glucoside | + | carbon source |
43437 | 63154 | N-acetyl-beta-D-mannosamine | + | carbon source |
43437 | 28037 | N-acetylgalactosamine | + | carbon source |
43437 | 506227 | N-acetylglucosamine | + | carbon source |
43437 | 17309 | pectin | + | carbon source |
43437 | 64103 | sodium butyrate | + | carbon source |
43437 | 75228 | sodium lactate | + | carbon source |
43437 | 17992 | sucrose | + | carbon source |
43437 | 4853 | esculin | + | hydrolysis |
43437 | 5291 | gelatin | + | hydrolysis |
43437 | 17632 | nitrate | + | reduction |
metabolite production
- @ref: 43437
- Chebi-ID: 89634
- metabolite: melanin
- production: yes
enzymes
@ref | value | activity | ec |
---|---|---|---|
43437 | cytochrome oxidase | + | 1.9.3.1 |
43437 | catalase | + | 1.11.1.6 |
43437 | alkaline phosphatase | + | 3.1.3.1 |
43437 | esterase (C 4) | + | |
43437 | esterase Lipase (C 8) | + | |
43437 | leucine arylamidase | + | 3.4.11.1 |
43437 | acid phosphatase | + | 3.1.3.2 |
43437 | naphthol-AS-BI-phosphohydrolase | + | |
43437 | alpha-glucosidase | + | 3.2.1.20 |
43437 | beta-glucosidase | + | 3.2.1.21 |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | latitude | longitude |
---|---|---|---|---|---|---|---|
43437 | Thal Desert | Thal Desert, Punjab | Pakistan | PAK | Asia | 31.383 | 71.434 |
64867 | desert soil | Thal desert, Punjab (31° 23' N, 71° 26' E) | Pakistan | PAK | Asia | 31.3833 | 71.4333 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Terrestrial | #Desert |
#Environmental | #Terrestrial | #Soil |
#Condition | #Xerophilic |
taxonmaps
- @ref: 69479
- File name: preview.99_73059.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_142;96_7914;97_39340;98_51172;99_73059&stattab=map
- Last taxonomy: Nocardioides thalensis subclade
- 16S sequence: LC193943
- Sequence Identity:
- Total samples: 219
- soil counts: 177
- aquatic counts: 4
- animal counts: 20
- plant counts: 18
Safety information
risk assessment
- @ref: 64867
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 64867
- description: Nocardioides sp. NCCP-696 gene for 16S ribosomal RNA, partial sequence
- accession: LC193943
- length: 1469
- database: ena
- NCBI tax ID: 1914755
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Nocardioides thalensis DSM 103833 | GCA_013410655 | contig | ncbi | 1914755 |
66792 | Nocardioides thalensis strain DSM 103833 | 1914755.3 | wgs | patric | 1914755 |
66792 | Nocardioides thalensis DSM 103833 | 2856476943 | draft | img | 1914755 |
GC content
- @ref: 64867
- GC-content: 71.6
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 85 | no |
motile | no | 77.373 | yes |
gram-positive | yes | 89.247 | yes |
anaerobic | no | 98.133 | no |
aerobic | yes | 89.879 | no |
halophile | no | 96.175 | no |
spore-forming | no | 74.628 | no |
glucose-util | yes | 88.318 | no |
flagellated | no | 93.795 | no |
thermophile | no | 94.959 | yes |
glucose-ferment | no | 86.95 | no |
External links
@ref: 64867
culture collection no.: CCTCC AB 2016296, DSM 103833, YIM 73001, NCCP 696
straininfo link
- @ref: 96775
- straininfo: 399181
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 28853683 | Nocardioides thalensis sp. nov., isolated from a desert. | Khan IU, Hussain F, Habib N, Xiao M, Ahmed I, Amin A, Zhi XY, Li WJ | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002032 | 2017 | Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Pakistan, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
Phylogeny | 30403584 | Nocardioides silvaticus sp. nov., isolated from forest soil. | Li C, Shi K, Zhang Y, Wang G | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003079 | 2018 | Actinomycetales/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, *Forests, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
Reference
@id | authors | title | doi/url | journal | pubmed | catalogue |
---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
43437 | Inam Ullah Khan, Firasat Hussain, Neeli Habib, Min Xiao, Iftikhar Ahmed, Arshia Amin, Xiao-Yang Zhi, Wen-Jun Li | Nocardioides thalensis sp. nov., isolated from a desert | 10.1099/ijsem.0.002032 | IJSEM 67: 2848-2852 2017 | 28853683 | |
64867 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-103833 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 103833) | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
96775 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID399181.1 |