Strain identifier

BacDive ID: 140671

Type strain: Yes

Species: Pseudonocardia thailandensis

Strain Designation: CMU-NKS-70

Strain history: S. Lumyong; Chiang Mai Univ., Thailand; CMU-NKS-70.

NCBI tax ID(s): 1701089 (species)

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General

@ref: 43435

BacDive-ID: 140671

keywords: 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive, rod-shaped

description: Pseudonocardia thailandensis CMU-NKS-70 is an aerobe, spore-forming, Gram-positive bacterium that was isolated from subterranean termite nest collected from a rubber tree plantation in Phayao Province.

NCBI tax id

  • NCBI tax id: 1701089
  • Matching level: species

strain history

  • @ref: 67770
  • history: S. Lumyong; Chiang Mai Univ., Thailand; CMU-NKS-70.

doi: 10.13145/bacdive140671.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Pseudonocardia
  • species: Pseudonocardia thailandensis
  • full scientific name: Pseudonocardia thailandensis Sujarit et al. 2017

@ref: 43435

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Pseudonocardiales

family: Pseudonocardiaceae

genus: Pseudonocardia

species: Pseudonocardia thailandensis

strain designation: CMU-NKS-70

type strain: yes

Morphology

cell morphology

  • @ref: 43435
  • gram stain: positive
  • cell shape: rod-shaped

colony morphology

  • @ref: 43435
  • colony color: pinkish cream, cream brown
  • medium used: ISP2 medium

Culture and growth conditions

culture medium

  • @ref: 43435
  • name: ISP2
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
43435positivegrowth15-40
43435positiveoptimum28-35mesophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
43435positivegrowth5-10alkaliphile
43435positiveoptimum7-9

Physiology and metabolism

oxygen tolerance

  • @ref: 43435
  • oxygen tolerance: aerobe

spore formation

  • @ref: 43435
  • spore description: rod-like, spore chains consist of two or more smooth spores
  • type of spore: spore
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
43435NaClpositivegrowth0-7 %(w/v)
43435NaClpositiveoptimum1-3 %(w/v)

observation

  • @ref: 67770
  • observation: quinones: MK-8(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4343565327D-xylose-builds acid from
4343517268myo-inositol-builds acid from
4343517716lactose-builds acid from
4343517306maltose-builds acid from
4343516947citrate-carbon source
4343515824D-fructose-carbon source
4343565327D-xylose-carbon source
4343516634raffinose-carbon source
4343526546rhamnose-carbon source
4343562968cellulose-hydrolysis
434355291gelatin-hydrolysis
4343517632nitrate-reduction
4343517634D-glucose+builds acid from
4343516899D-mannitol+builds acid from
4343516988D-ribose+builds acid from
4343517924D-sorbitol+builds acid from
4343530849L-arabinose+builds acid from
4343537684mannose+builds acid from
4343526546rhamnose+builds acid from
4343530911sorbitol+builds acid from
4343517992sucrose+builds acid from
4343516899D-mannitol+carbon source
4343530849L-arabinose+carbon source
4343517992sucrose+carbon source
4343516467L-arginine+nitrogen source
4343518019L-lysine+nitrogen source
4343515729L-ornithine+nitrogen source
4343517268myo-inositol+/-carbon source

metabolite production

@refChebi-IDmetaboliteproduction
4343516136hydrogen sulfideno
4343589634melaninno

enzymes

@refvalueactivityec
43435urease+3.5.1.5
43435catalase+1.11.1.6
43435cytochrome oxidase+1.9.3.1
43435trypsin-3.4.21.4
43435alpha-chymotrypsin-3.4.21.1
43435alpha-galactosidase-3.2.1.22
43435beta-galactosidase-3.2.1.23
43435beta-glucuronidase-3.2.1.31
43435beta-glucosidase-3.2.1.21
43435N-acetyl-beta-glucosaminidase-3.2.1.52
43435alpha-mannosidase-3.2.1.24
43435alpha-fucosidase-3.2.1.51
43435alkaline phosphatase+3.1.3.1
43435esterase (C 4)+
43435esterase Lipase (C 8)+
43435lipase (C 14)+
43435leucine arylamidase+3.4.11.1
43435valine arylamidase+
43435cystine arylamidase+3.4.11.3
43435acid phosphatase+3.1.3.2
43435naphthol-AS-BI-phosphohydrolase+
43435alpha-glucosidase+3.2.1.20

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
43435subterranean termite nest collected from a rubber tree plantation in Phayao ProvincePhayao Province, ThailandThailandTHAAsia
67770Subterranean termite nestsThailandTHAAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Plantation
#Host#Arthropoda#Insecta
#Host#Plants#Tree

taxonmaps

  • @ref: 69479
  • File name: preview.99_105670.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_2672;97_3280;98_12070;99_105670&stattab=map
  • Last taxonomy: Pseudonocardia thailandensis
  • 16S sequence: LC073313
  • Sequence Identity:
  • Total samples: 39
  • soil counts: 28
  • aquatic counts: 4
  • animal counts: 5
  • plant counts: 2

Sequence information

16S sequences

@refdescriptionaccessiondatabaselengthNCBI tax ID
4343516S rRNA gene sequenceLC073313nuccore
67770Pseudonocardia thailandensis strain CMU-NKS-70 16S ribosomal RNA gene, partial sequenceKF746342ena13421701089

GC content

  • @ref: 43435
  • GC-content: 71.9
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 43435

culture collection no.: JCM 31292, TBRC 2000

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny28853680Pseudonocardia thailandensis sp. nov., an actinomycete isolated from a subterranean termite nest.Sujarit K, Sujada N, Kudo T, Ohkuma M, Pathom-Aree W, Lumyong SInt J Syst Evol Microbiol10.1099/ijsem.0.0020172017Actinomycetales/*classification/genetics/isolation & purification, Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Glycolipids/chemistry, Isoptera/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Thailand, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny33890128Pseudonocardia pini sp. nov., an endophytic actinobacterium isolated from roots of the pine tree Callitris preissii.Kaewkla O, Franco CMMArch Microbiol10.1007/s00203-021-02309-32021*Cupressaceae/microbiology, Fatty Acids/analysis, Nucleic Acid Hybridization, Phylogeny, *Plant Roots/microbiology, *Pseudonocardia/classification/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species SpecificityTranscriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43435Kanaporn Sujarit, Nikhom Sujada, Takuji Kudo, Moriya Ohkuma, Wasu Pathom-Aree, Saisamorn LumyongPseudonocardia thailandensis sp. nov., an actinomycete isolated from a subterranean termite nest10.1099/ijsem.0.002017IJSEM 67: 2773-2778 201728853680
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/