Strain identifier

BacDive ID: 140648

Type strain: Yes

Species: Salinarchaeum chitinilyticum

Strain Designation: MC-74

Strain history: H. Minegishi MC-74.

NCBI tax ID(s): 1395169 (species)

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General

@ref: 43289

BacDive-ID: 140648

keywords: 16S sequence, Archaea, halophilic, thermophilic, Gram-negative, motile, rod-shaped, colony-forming

description: Salinarchaeum chitinilyticum MC-74 is a halophilic, thermophilic, Gram-negative archaeon that forms circular colonies and was isolated from commercial salt samples.

NCBI tax id

  • NCBI tax id: 1395169
  • Matching level: species

strain history

  • @ref: 67770
  • history: H. Minegishi MC-74.

doi: 10.13145/bacdive140648.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Halobacteria
  • order: Natrialbales
  • family: Natrialbaceae
  • genus: Salinarchaeum
  • species: Salinarchaeum chitinilyticum
  • full scientific name: Salinarchaeum chitinilyticum Minegishi et al. 2017

@ref: 43289

domain: Archaea

phylum: Euryarchaeota

class: Halobacteria

order: Natrialbales

family: Natrialbaceae

genus: Salinarchaeum

species: Salinarchaeum chitinilyticum

strain designation: MC-74

type strain: yes

Morphology

cell morphology

  • @ref: 43289
  • gram stain: negative
  • cell length: 1.2-2.3 µm
  • cell width: 0.2-0.3 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 43289
  • colony color: vermillion
  • colony shape: circular

Culture and growth conditions

culture temp

@refgrowthtypetemperaturerange
43289positiveoptimum40thermophilic
43289positivegrowth20-50
67770positivegrowth37mesophilic

culture pH

@refabilitytypepHPH range
43289positivegrowth6.0-10.0alkaliphile
43289positiveoptimum7

Physiology and metabolism

halophily

@refhalophily levelsaltgrowthtested relationconcentration
43289halophilicNaClpositivegrowth1.5-5.1 M
43289NaClpositiveoptimum2.6-3.1 M

observation

@refobservation
43289Cells are lysed in distilled water
43289chitin degradation

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4328918420magnesium(2+)-required for growth
4328916301nitrite+reduction
43289casein-hydrolysis
432895291gelatin-hydrolysis
4328928017starch-hydrolysis
43289skimmed milk-hydrolysis
4328953426tween 80-hydrolysis
4328915824D-fructose+carbon source
4328917634D-glucose+carbon source
4328917716lactose+carbon source
4328917306maltose+carbon source
4328916024D-mannose+carbon source
4328916634raffinose+carbon source
4328915963ribitol+carbon source
4328933942ribose+carbon source
4328917924D-sorbitol+carbon source
4328927082trehalose+carbon source
4328932954sodium acetate+carbon source
43289115156disodium fumarate+carbon source
4328950144sodium pyruvate+carbon source
4328963675sodium succinate+carbon source
4328930849L-arabinose-carbon source
4328917754glycerol-carbon source
4328927907alpha-L-rhamnose-carbon source
4328965327D-xylose-carbon source
4328953258sodium citrate-carbon source
4328924996lactate-carbon source
4328916977L-alanine-carbon source
4328964243sodium L-glutamate-carbon source
4328915428glycine-carbon source
4328918019L-lysine-carbon source
4328991261disodium (s)-malate-carbon source
4328917057cellobiose+/-carbon source
4328912936D-galactose+/-carbon source
4328917266L-sorbose+/-carbon source
4328917992sucrose+/-carbon source
4328917317D-sorbose+carbon source

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.group ID
43289338412(-)-anisomycinyesyes50 µg (disc)
4328928971ampicillinyesyes10 µg (disc)
432892766aphidicolinyesyes50 µg (disc)
4328928669bacitracinyesyes10 Unit (disc)
4328917698chloramphenicolyesyes30 µg (disc)
4328948923erythromycinyesyes15 µg (disc)
4328917833gentamicinyesyes120 µg (disc)
432896104kanamycinyesyes30 µg (disc)
43289100147nalidixic acidyesyes30 µg (disc)
432897507neomycinyesyes30 µg (disc)
4328928368novobiocinyesyes30 µg (disc)
432897660nystatinyesyes50 µg (disc)
4328918208penicillin gyesyes10 Unit (disc)
43289piperacillinyesyes5 µg (disc)16
43289tazobactamyesyes5 µg (disc)16
432898309polymyxin byesyes5 µg (disc)
4328928077rifampicinyesyes5 µg (disc)
4328917076streptomycinyesyes300 µg (disc)
43289102484sulfisoxazoleyesyes5 µg (disc)
4328927902tetracyclineyesyes30 µg (disc)
4328928001vancomycinyesyes30 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
4328916136hydrogen sulfideno
4328935581indoleno

enzymes

@refvalueactivityec
43289catalase-1.11.1.6
43289cytochrome oxidase-1.9.3.1

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
43289commercial salt samplesFranceFranceFRAEurope
67770Solar salt in France

isolation source categories

  • Cat1: #Condition
  • Cat2: #Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_188025.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17921;96_68788;97_89430;98_121700;99_188025&stattab=map
  • Last taxonomy: Salinarchaeum
  • 16S sequence: AB850662
  • Sequence Identity:
  • Total samples: 20
  • soil counts: 7
  • aquatic counts: 10
  • animal counts: 3

Sequence information

16S sequences

  • @ref: 43289
  • description: 16S rRNA gene sequences
  • accession: AB850662
  • length: 1471
  • database: ena
  • NCBI tax ID: 1395169

GC content

  • @ref: 67770
  • GC-content: 68
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 43289

culture collection no.: JCM 19597, KCTC 4262

literature

  • topic: Phylogeny
  • Pubmed-ID: 28671534
  • title: Salinarchaeum chitinilyticum sp. nov., a chitin-degrading haloarchaeon isolated from commercial salt.
  • authors: Minegishi H, Enomoto S, Echigo A, Shimane Y, Kondo Y, Inoma A, Kamekura M, Takai K, Itoh T, Ohkuma M, Ihara K, Takahashi-Ando N, Fukushima Y, Ishii S, Yoshida Y, Usami R
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001941
  • year: 2017
  • mesh: China, Chitin/metabolism, DNA, Archaeal/genetics, France, Glycolipids/chemistry, Halobacteriaceae/*classification/genetics/isolation & purification, Neisseriaceae/genetics, Nucleic Acid Hybridization, Phosphatidylglycerols/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/*analysis
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43289Hiroaki Minegishi, Shigeaki Enomoto, Akinobu Echigo, Yasuhiro Shimane, Yusuke Kondo, Ayumi Inoma, Masahiro Kamekura, Ken Takai, Takashi Itoh, Moriya Ohkuma, Kunio Ihara, Naoko Takahashi-Ando, Yasumasa Fukushima, Shigeru Ishii, Yasuhiko Yoshida and Ron UsamiSalinarchaeum chitinilyticum sp. nov., a chitin-degrading haloarchaeon isolated from commercial salt10.1099/ijsem.0.001941IJSEM 67: 2274-2278 201728671534
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/