Strain identifier

BacDive ID: 140637

Type strain: Yes

Species: Mycobacterium intracellulare

Strain Designation: MOTT64

Strain history: B.-J. Kim; Seoul Natl. Univ. Coll. of Med., South Korea; MOTT-64.

NCBI tax ID(s): 1138383 (species)

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General

@ref: 43072

BacDive-ID: 140637

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, rod-shaped

description: Mycobacterium intracellulare MOTT64 is a mesophilic, rod-shaped bacterium that was isolated from human sputum .

NCBI tax id

  • NCBI tax id: 1138383
  • Matching level: species

strain history

  • @ref: 67770
  • history: B.-J. Kim; Seoul Natl. Univ. Coll. of Med., South Korea; MOTT-64.

doi: 10.13145/bacdive140637.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium intracellulare
  • full scientific name: Mycobacterium intracellulare corrig. (Cuttino and McCabe 1949) Runyon 1965 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Mycobacterium intracellularis
    20215Mycobacterium paraintracellulare
    20215Nocardia intracellularis

@ref: 43072

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Mycobacteriales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium paraintracellulare

strain designation: MOTT64

type strain: yes

Morphology

cell morphology

  • @ref: 43072
  • cell shape: rod-shaped

colony morphology

  • @ref: 43072
  • colony color: white-yellow
  • incubation period: >7 days
  • medium used: Middlebrook 7H10 agar

Culture and growth conditions

culture medium

@refnamegrowthcomposition
43072Middlebrook 7H10 agaryes
43072MacConkey agaryeswithout crystal violet
430727H10 agaryeswith thiophene-2-carboxylic acid hydrazide (10 µg/ml)
430727H10 agaryeswith p-nitrobenzoic acid (500 µg/ml)
430727H10 agarnoethambutol (8 µg/ml)
430727H10 agarno5 % NaCl
430727H9 brothyes

culture temp

@refgrowthtypetemperaturerange
43072positivegrowth25mesophilic
43072positiveoptimum37mesophilic
67770positivegrowth37mesophilic

culture pH

  • @ref: 43072
  • ability: positive
  • type: growth
  • pH: 5.5

Physiology and metabolism

spore formation

  • @ref: 43072
  • type of spore: spore
  • spore formation: no

metabolite utilization

  • @ref: 43072
  • Chebi-ID: 17632
  • metabolite: nitrate
  • utilization activity: -
  • kind of utilization tested: reduction

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.is sensitivesensitivity conc.
430722637amikacinyesyes16 µg/mL
430722637amikacinyesyes32 µg/mL
43072209807cefoxitinyesyes256 µg/mL
43072100241ciprofloxacinyesyes16 µg/mL
430723732clarithromycinyesyes2 µg/mL
4307250845doxycyclineyesyes32 µg/mL
43072471744imipenemyesyes64 µg/mL
4307263611moxifloxacinyesyes2 µg/mL
4307263611moxifloxacinyesyes4 µg/mL
4307228077rifampicinyesyes4 µg/mL
4307228864tobramycinyesyes8 µg/mL

enzymes

@refvalueactivityec
43072catalase+1.11.1.6
43072pyrazinamidase+3.5.1.B15
43072tellurite reductase+
43072arylsulfatase-3.1.6.1
43072urease-3.5.1.5
43072lipase (C 14)+/-
43072valine arylamidase+/-
43072cystine arylamidase+/-3.4.11.3
43072alkaline phosphatase+3.1.3.1
43072esterase (C 4)+
43072esterase Lipase (C 8)+
43072leucine arylamidase+3.4.11.1
43072acid phosphatase+3.1.3.2
43072naphthol-AS-BI-phosphohydrolase+
43072beta-glucosidase+3.2.1.21

Isolation, sampling and environmental information

isolation

@refsample typesampling dategeographic locationcountryorigin.countrycontinent
43072human sputum (60-year-old male pulmonary patient)2010Seoul, Republic of KoreaRepublic of KoreaKORAsia
67770Sputum of a patient with pulmonary infectionsSeoulRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body Product#Fluids#Sputum

taxonmaps

  • @ref: 69479
  • File name: preview.99_402.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_37;97_39;98_43;99_402&stattab=map
  • Last taxonomy: Mycobacterium
  • 16S sequence: JF271821
  • Sequence Identity:
  • Total samples: 24078
  • soil counts: 12779
  • aquatic counts: 4789
  • animal counts: 3677
  • plant counts: 2833

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
4307216S rRNA gene sequencesKP6703291305ena1138383
67770Mycobacterium sp. MOTT-64 16S ribosomal RNA gene, partial sequenceJF2718211395ena1069226
67770Mycobacterium sp. MOTT-64 16S-23S ribosomal RNA intergenic spacer, partial sequenceJQ411530280ena1069226

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycobacterium paraintracellulare JCM 30622GCA_010731935completencbi1138383
66792Mycobacterium paraintracellulare strain JCM 306221138383.15completepatric1138383
66792Mycobacterium paraintracellulare strain KCTC 290841138383.9wgspatric1138383
67770Mycobacterium paraintracellulare MOTT64GCA_000276825completencbi1138383
67770Mycobacterium paraintracellulare KCTC 29084GCA_002104735contigncbi1138383

GC content

  • @ref: 67770
  • GC-content: 68.07
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno94.54no
flagellatedno98.936no
gram-positiveyes91.799no
anaerobicno99.498no
aerobicyes90.004no
halophileno91.554no
spore-formingno85.268yes
glucose-utilyes65.476no
thermophileno98.212yes
glucose-fermentno90.749no

External links

@ref: 43072

culture collection no.: KCTC 29084, JCM 30622

literature

  • topic: Phylogeny
  • Pubmed-ID: 27189351
  • title: Mycobacterium paraintracellulare sp. nov., for the genotype INT-1 of Mycobacterium intracellulare.
  • authors: Lee SY, Kim BJ, Kim H, Won YS, Jeon CO, Jeong J, Lee SH, Lim JH, Lee SH, Kim CK, Kook YH, Kim BJ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001158
  • year: 2016
  • mesh: Aged, Aged, 80 and over, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, DNA, Ribosomal Spacer/genetics, Female, Genes, Bacterial, Humans, Male, Middle Aged, Multilocus Sequence Typing, Mycobacterium Infections/microbiology, Mycobacterium avium Complex/*classification/genetics/isolation & purification, Mycolic Acids/chemistry, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Sputum/*microbiology
  • topic2: Pathogenicity

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43072So-Young Lee, Byoung-Jun Kim, Hong Kim, Yu-Seop Won, Che Ok Jeon, Joseph Jeong, Seon Ho Lee, Ji-Hun Lim, Seung-Heon Lee, Chang Ki Kim, Yoon-Hoh Kook and Bum-Joon KimMycobacterium paraintracellulare sp. nov., for the genotype INT-1 of Mycobacterium intracellulare10.1099/ijsem.0.001158IJSEM 66: 3132-3141 201627189351
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/