Strain identifier

BacDive ID: 14063

Type strain: Yes

Species: Shewanella woodyi

Strain Designation: MS32

Strain history: CIP <- 2000, J.C. Makemson, Florida Int. Univ., USA: strain MS32

NCBI tax ID(s): 60961 (species)

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General

@ref: 4584

BacDive-ID: 14063

DSM-Number: 12036

keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, motile, rod-shaped

description: Shewanella woodyi MS32 is a facultative anaerobe, mesophilic, Gram-negative bacterium that was isolated from detritus at a depth of 370 m.

NCBI tax id

  • NCBI tax id: 60961
  • Matching level: species

strain history

@refhistory
4584<- J.C. Makemson, Florida International Univ., Miami USA, MS32
340492000, J.C. Makemson, Florida Int. Univ., USA: strain MS32
123958CIP <- 2000, J.C. Makemson, Florida Int. Univ., USA: strain MS32

doi: 10.13145/bacdive14063.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Alteromonadales
  • family: Shewanellaceae
  • genus: Shewanella
  • species: Shewanella woodyi
  • full scientific name: Shewanella woodyi Makemson et al. 1997

@ref: 4584

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Alteromonadales

family: Shewanellaceae

genus: Shewanella

species: Shewanella woodyi

full scientific name: Shewanella woodyi Makemson et al. 1997

strain designation: MS32

type strain: yes

Morphology

cell morphology

  • @ref: 123958
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4584BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
34049Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
123958CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
4584positivegrowth25mesophilic
34049positivegrowth25mesophilic
123958positivegrowth10-30
123958nogrowth5psychrophilic
123958nogrowth37mesophilic
123958nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 123958
  • oxygen tolerance: facultative anaerobe

halophily

@refsaltgrowthtested relationconcentration
123958NaClpositivegrowth2-6 %
123958NaClnogrowth0 %
123958NaClnogrowth8 %
123958NaClnogrowth10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
123958citrate-carbon source16947
123958esculin-hydrolysis4853
123958nitrate+reduction17632
123958nitrite-reduction16301
123958nitrate+respiration17632

antibiotic resistance

  • @ref: 123958
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

  • @ref: 123958
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
123958oxidase+
123958beta-galactosidase-3.2.1.23
123958alcohol dehydrogenase-1.1.1.1
123958gelatinase-
123958amylase-
123958DNase+
123958caseinase-3.4.21.50
123958catalase+1.11.1.6
123958tween esterase+
123958lecithinase-
123958lipase-
123958lysine decarboxylase-4.1.1.18
123958ornithine decarboxylase-4.1.1.17
123958protease-
123958tryptophan deaminase-
123958urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123958-+++-+--+-+------+--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123958-------------------------------------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123958+++-+--------------+-----------+-----------------+---------++--------------+---++-----+--+++-++----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryisolation date
4584detritus at a depth of 370 mMediterranean, Alboran Sea
123958Detritus, luminous, 370 m depthAlboran Sea1993

isolation source categories

Cat1Cat2Cat3
#Host#Other#Decomposing animal
#Host#Plants#Decomposing plant

taxonmaps

  • @ref: 69479
  • File name: preview.99_1365.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_59;96_479;97_547;98_1078;99_1365&stattab=map
  • Last taxonomy: Shewanella woodyi subclade
  • 16S sequence: AF003549
  • Sequence Identity:
  • Total samples: 985
  • soil counts: 11
  • aquatic counts: 828
  • animal counts: 145
  • plant counts: 1

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
45841Risk group (German classification)
1239581Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Shewanella woodyi 16S ribosomal RNA gene, complete sequence
  • accession: AF003549
  • length: 1443
  • database: ena
  • NCBI tax ID: 392500

External links

@ref: 4584

culture collection no.: DSM 12036, CIP 105547, ATCC 51908

straininfo link

  • @ref: 83232
  • straininfo: 12781

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4584Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12036)https://www.dsmz.de/collection/catalogue/details/culture/DSM-12036
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34049Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17579
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
83232Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID12781.1StrainInfo: A central database for resolving microbial strain identifiers
123958Curators of the CIPCollection of Institut Pasteur (CIP 105547)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105547