Strain identifier

BacDive ID: 140618

Type strain: Yes

Species: Paenibacillus aceti

Strain Designation: L14

Strain history: L. Luo; South China Univ. of Technol., China; L14.

NCBI tax ID(s): 1820010 (species)

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General

@ref: 43136

BacDive-ID: 140618

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, spore-forming, mesophilic, Gram-negative, motile, rod-shaped

description: Paenibacillus aceti L14 is a facultative anaerobe, spore-forming, mesophilic bacterium that was isolated from from traditional acetic acid fermentation culture of cereal vinegars.

NCBI tax id

  • NCBI tax id: 1820010
  • Matching level: species

strain history

  • @ref: 67770
  • history: L. Luo; South China Univ. of Technol., China; L14.

doi: 10.13145/bacdive140618.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Paenibacillus
  • species: Paenibacillus aceti
  • full scientific name: Paenibacillus aceti Li et al. 2016

@ref: 43136

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Paenibacillaceae

genus: Paenibacillus

species: Paenibacillus aceti

strain designation: L14

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
43136negative1.0-8.5 µm0.2-0.3 µmrod-shapedyes
69480yes98.186
69480positive100

colony morphology

  • @ref: 43136
  • colony size: 0.5-1.0 mm
  • colony color: cream-white
  • incubation period: 3 days
  • medium used: TSA

Culture and growth conditions

culture medium

  • @ref: 43136
  • name: Trypticase Soy Agar (TSA)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
43136positiveoptimum37mesophilic
43136nogrowth55thermophilic
43136nogrowth10psychrophilic
43136positivegrowth15-50
67770positivegrowth37mesophilic

culture pH

@refabilitytypepHPH range
43136positivegrowth4-10alkaliphile
43136positiveoptimum8

Physiology and metabolism

oxygen tolerance

  • @ref: 43136
  • oxygen tolerance: facultative anaerobe

spore formation

@reftype of sporespore formationconfidence
43136endosporeyes
69481yes100
69480yes100

halophily

@refsaltgrowthtested relationconcentration
43136NaClpositivegrowth0-8 %
43136NaClpositiveoptimum0.5 %
43136NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4313616947citrate+assimilation
4313617234glucose+fermentation
4313617632nitrate-reduction
4313617634D-glucose+builds acid from
4313617754glycerol+builds acid from
4313630849L-arabinose+builds acid from
4313616988D-ribose+builds acid from
4313665327D-xylose+builds acid from
4313612936D-galactose+builds acid from
4313615824D-fructose+builds acid from
4313616024D-mannose+builds acid from
4313617317D-sorbose+builds acid from
4313662345L-rhamnose+builds acid from
4313616899D-mannitol+builds acid from
4313643943methyl alpha-D-mannoside+builds acid from
43136320061methyl alpha-D-glucopyranoside+builds acid from
43136506227N-acetylglucosamine+builds acid from
4313627613amygdalin+builds acid from
4313618305arbutin+builds acid from
43136esculin ferric citrate+builds acid from
4313617814salicin+builds acid from
4313617057cellobiose+builds acid from
4313617306maltose+builds acid from
4313636219alpha-lactose+builds acid from
4313617992sucrose+builds acid from
4313627082trehalose+builds acid from
4313615443inulin+builds acid from
4313628017starch+builds acid from
4313628066gentiobiose+builds acid from
4313632528turanose+builds acid from
4313662318D-lyxose+builds acid from
4313632032potassium gluconate+builds acid from
43136potassium 5-dehydro-D-gluconate+builds acid from
4313617925alpha-D-glucose+respiration
4313627082trehalose+respiration
4313617057cellobiose+respiration
4313628066gentiobiose+respiration
4313628037N-acetylgalactosamine+respiration
4313618333D-arabitol+respiration
4313673706bromosuccinate+respiration
4313616634raffinose-builds acid from
4313674863methyl beta-D-xylopyranoside-builds acid from

antibiotic resistance

@refChEBImetaboliteis antibioticis resistant
43136161680aztreonamyesyes
4313671321fusidateyesyes
4313632735guanidinium chlorideyesyes
431366472lincomycinyesyes
4313629673rifamycin svyesyes
4313675198tetrazolium blueyesyes
4313645735troleandomycinyesyes
4313628001vancomycinyesyes

metabolite production

@refChebi-IDmetaboliteproduction
4313615688acetoinyes
4313616136hydrogen sulfideno
4313635581indoleno

metabolite tests

@refChebi-IDmetabolitemethylred-testvoges-proskauer-test
4313617234glucose+
4313615688acetoin+

enzymes

@refvalueactivityec
43136beta-galactosidase+3.2.1.23
43136lysine decarboxylase+4.1.1.18
43136catalase-1.11.1.6
43136cytochrome oxidase-1.9.3.1
43136urease-3.5.1.5
43136alkaline phosphatase+3.1.3.1
43136esterase Lipase (C 8)+
43136leucine arylamidase+3.4.11.1
43136valine arylamidase+
43136cystine arylamidase+3.4.11.3
43136trypsin+3.4.21.4
43136alpha-chymotrypsin+3.4.21.1
43136acid phosphatase+3.1.3.2
43136naphthol-AS-BI-phosphohydrolase+
43136beta-glucosidase+3.2.1.21

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43136C14:01.3
    43136C15:02.1
    43136C16:011.7
    43136C14:0 iso2.1
    43136C15:0 iso12.3
    43136C16:0 iso7.9
    43136C17:0 iso9.5
    43136C15:0 anteiso32.5
    43136C17:0 anteiso8.2
  • type of FA analysis: whole cell analysis
  • incubation medium: TSB
  • incubation temperature: 37
  • incubation time: 2
  • software version: Sherlock 6.0
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
43136from traditional acetic acid fermentation culture of cereal vinegarsShanxi, ChinaChinaCHNAsia
67770Traditional acetic acid fermentation culture of Chinese cereal vinegars

isolation source categories

Cat1Cat2Cat3
#Condition#Acidic
#Engineered#Bioreactor
#Engineered#Food production#Fermented
#Engineered#Food production#Beverage

taxonmaps

  • @ref: 69479
  • File name: preview.99_26798.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15358;96_1149;97_1349;98_20088;99_26798&stattab=map
  • Last taxonomy: Paenibacillus aceti subclade
  • 16S sequence: KU050693
  • Sequence Identity:
  • Total samples: 86
  • soil counts: 28
  • aquatic counts: 4
  • animal counts: 46
  • plant counts: 8

Sequence information

16S sequences

  • @ref: 43136
  • description: 16S rRNA gene sequences
  • accession: KU050693
  • length: 1515
  • database: ena
  • NCBI tax ID: 1820010

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Paenibacillus aceti CGMCC 1.15420GCA_014642755scaffoldncbi1820010
66792Paenibacillus aceti strain CGMCC 1.154201820010.4wgspatric1820010
66792Paenibacillus aceti L142882253857draftimg1820010
67770Paenibacillus aceti L14GCA_003614185scaffoldncbi1820010

GC content

  • @ref: 43136
  • GC-content: 49.9
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
flagellatedyes72.381no
gram-positiveyes72.397yes
anaerobicno96.727yes
aerobicyes57.183yes
halophileno91.611no
spore-formingyes94.961yes
glucose-utilyes89.812yes
thermophileno99.38no
motileyes89.624yes
glucose-fermentno70.964yes

External links

@ref: 43136

culture collection no.: CGMCC 1.15420, JCM 31170

literature

  • topic: Phylogeny
  • Pubmed-ID: 27265263
  • title: Paenibacillus aceti sp. nov., isolated from the traditional solid-state acetic acid fermentation culture of Chinese cereal vinegar.
  • authors: Li P, Lin W, Liu X, Li S, Luo L, Lin WT
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001214
  • year: 2016
  • mesh: Acetic Acid/chemistry, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Edible Grain/*microbiology, Fatty Acids/chemistry, *Fermentation, Nucleic Acid Hybridization, Paenibacillus/*classification/genetics/isolation & purification, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43136Pan Li, Weifeng Lin, Xiong Liu, Sha Li, Lixin Luo and Wei-Tie LinPaenibacillus aceti sp. nov., isolated from the traditional solid-state acetic acid fermentation culture of Chinese cereal vinegar10.1099/ijsem.0.001214IJSEM 66: 3426-3431 201627265263
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1