Strain identifier

BacDive ID: 140606

Type strain: Yes

Species: Acinetobacter defluvii

Strain Designation: WCHA30

Strain history: <- KCTC; KCTC 52503 <- Y. Hu, West China Hospital of Sichuan University; WCHA30

NCBI tax ID(s): 1871111 (species)

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General

@ref: 64855

BacDive-ID: 140606

DSM-Number: 107594

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, thermophilic, Gram-negative, coccus-shaped, colony-forming

description: Acinetobacter defluvii WCHA30 is an obligate aerobe, thermophilic, Gram-negative bacterium that forms circular colonies and was isolated from hospital sewage.

NCBI tax id

  • NCBI tax id: 1871111
  • Matching level: species

strain history

  • @ref: 64855
  • history: <- KCTC; KCTC 52503 <- Y. Hu, West China Hospital of Sichuan University; WCHA30

doi: 10.13145/bacdive140606.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Acinetobacter
  • species: Acinetobacter defluvii
  • full scientific name: Acinetobacter defluvii Hu et al. 2017

@ref: 64855

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Acinetobacter

species: Acinetobacter defluvii

full scientific name: Acinetobacter defluvii Hu et al. 2017

strain designation: WCHA30

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
43264negative0.7-1.0 µm0.6-0.9 µmcoccus-shapedno
69480negative99.989

colony morphology

  • @ref: 43264
  • colony size: 0.8-1.2 mm
  • colony color: light yellow
  • colony shape: circular
  • incubation period: 1 day
  • medium used: TSA

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43264Trypticase Soy Agar (TSA)yes
43264LB (Luria-Bertani) MEDIUMyes
43264brain heart infusion agaryes
43264Müller-Hinton agaryes
64855NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
64855COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base

culture temp

@refgrowthtypetemperaturerange
43264nogrowth41-44thermophilic
43264positivegrowth20-37
64855positivegrowth30mesophilic

culture pH

  • @ref: 43264
  • ability: positive
  • type: growth
  • pH: 5-10
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 43264
  • oxygen tolerance: obligate aerobe

spore formation

@reftype of sporespore formationconfidence
43264sporeno
69481no100
69480no99.968

halophily

  • @ref: 43264
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-3 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4326417634D-glucose-builds acid from
432645291gelatin-hydrolysis
4326453423tween 40+hydrolysis
4326430089acetate+carbon source
4326416958beta-alanine+carbon source
4326416865gamma-aminobutyric acid+carbon source
4326416150benzoate+carbon source
4326416236ethanol+carbon source
4326424996lactate+carbon source
4326418401phenylacetate+carbon source
4326418123trigonelline+carbon source
4326415708trans-aconitate-carbon source
4326417128adipate-carbon source
4326430849L-arabinose-carbon source
4326416467L-arginine-carbon source
4326429991L-aspartate-carbon source
4326478208azelaate-carbon source
43264620642,3-butanediol-carbon source
4326430719citraconate-carbon source
4326416947citrate-carbon source
4326458044gentisate-carbon source
432648391D-gluconate-carbon source
4326417634D-glucose-carbon source
4326429985L-glutamate-carbon source
4326430921glutarate-carbon source
4326418295histamine-carbon source
4326415971L-histidine-carbon source
43264178794-hydroxybenzoate-carbon source
4326415603L-leucine-carbon source
4326415588D-malate-carbon source
4326415792malonate-carbon source
4326415729L-ornithine-carbon source
4326417295L-phenylalanine-carbon source
4326417148putrescine-carbon source
4326416988D-ribose-carbon source
4326430924L-tartrate-carbon source
4326462517tricarballylate-carbon source
4326416765tryptamine-carbon source
43264391504-oxopentanoate-carbon source
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
43264catalase+1.11.1.6
43264cytochrome oxidase-1.9.3.1
68369cytochrome oxidase-1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
64855+------+/-+/-------+-+---
64855---------------+-+---

Isolation, sampling and environmental information

isolation

@refsample typesampling dategeographic locationcountryorigin.countrycontinentlatitudelongitude
43264hospital sewage2015-11Chengdu, ChinaChinaCHNAsia
64855wastewater treatment plant of hospitalChengduChinaCHNAsia30.6667104.067

isolation source categories

Cat1Cat2Cat3
#Engineered#Waste#Sewage sludge
#Environmental#Aquatic
#Infection#Medical environment#Clinic

Safety information

risk assessment

  • @ref: 64855
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 43264
  • description: 16S rRNA gene sequences
  • accession: KY435933
  • length: 1546
  • database: ena
  • NCBI tax ID: 1871111

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acinetobacter defluvii WCHA30GCA_001704615completencbi1871111
66792Acinetobacter defluvii WCHA302767802242draftimg1871111

GC content

  • @ref: 43264
  • GC-content: 38

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno94.441yes
flagellatedno94.441yes
flagellatedno94.441yes
gram-positiveno97.799yes
gram-positiveno97.799yes
gram-positiveno97.799yes
anaerobicno97.818yes
anaerobicno97.818yes
anaerobicno97.818yes
halophileno90.324no
halophileno90.324no
halophileno90.324no
spore-formingno96.077yes
spore-formingno96.077yes
spore-formingno96.077yes
glucose-utilno91.31yes
glucose-utilno91.31yes
glucose-utilno91.31yes
aerobicyes89.302yes
aerobicyes89.302yes
aerobicyes89.302yes
thermophileno99.882no
thermophileno99.882no
thermophileno99.882no
motileno89.699yes
motileno89.699yes
motileno89.699yes
glucose-fermentno93.183yes
glucose-fermentno93.183yes
glucose-fermentno93.183yes

External links

@ref: 64855

culture collection no.: KCTC 52503, GDMCC 1.1101, CCTCC AB 2016203, DSM 107594

straininfo link

  • @ref: 96753
  • straininfo: 406554

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny28211316Acinetobacter defluvii sp. nov., recovered from hospital sewage.Hu Y, Feng Y, Zhang X, Zong ZInt J Syst Evol Microbiol10.1099/ijsem.0.0018472017Acinetobacter/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Hospitals, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiologyTranscriptome
Phylogeny31792599Acinetobacter pullicarnis sp. nov. isolated from chicken meat.Han RH, Lee JE, Yoon SH, Kim GBArch Microbiol10.1007/s00203-019-01785-y2019Acinetobacter/*classification/*genetics/isolation & purification, Animals, Base Composition, Chickens/*microbiology, DNA, Bacterial/genetics, Fatty Acids/analysis, Meat/*microbiology, Nucleic Acid Hybridization, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNATranscriptome

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43264Yiyi Hu, Yu Feng, Xiaoxia Zhang and Zhiyong ZongAcinetobacter defluvii sp. nov., recovered from hospital sewage10.1099/ijsem.0.001847IJSEM 67: 1709-1713 201728211316
64855Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-107594Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 107594)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
68369Automatically annotated from API 20NE
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
96753Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID406554.1