Strain identifier

BacDive ID: 140589

Type strain: Yes

Species: Saccharopolyspora spongiae

NCBI tax ID(s): 1847949 (species)

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General

@ref: 43230

BacDive-ID: 140589

DSM-Number: 103218

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Saccharopolyspora spongiae DSM 103218 is an aerobe, spore-forming, mesophilic bacterium that builds a substrate mycelium and was isolated from marine sponge Scopalina ruetzleri.

NCBI tax id

  • NCBI tax id: 1847949
  • Matching level: species

doi: 10.13145/bacdive140589.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Saccharopolyspora
  • species: Saccharopolyspora spongiae
  • full scientific name: Saccharopolyspora spongiae Souza et al. 2017

@ref: 43230

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Pseudonocardiales

family: Pseudonocardiaceae

genus: Saccharopolyspora

species: Saccharopolyspora spongiae

type strain: yes

Morphology

cell morphology

  • @ref: 43230
  • gram stain: positive

colony morphology

  • @ref: 43230
  • colony color: pale yellow
  • medium used: ISP2 medium

multicellular morphology

  • @ref: 43230
  • forms multicellular complex: yes
  • complex name: substrate mycelium
  • further description: extensively branched substrate mycelium

pigmentation

  • @ref: 43230
  • production: no
  • name: Diffusible pigments

Culture and growth conditions

culture medium

@refnamegrowth
43230ISP2 mediumyes
43230potato-dextrose agaryes
43230Czapek's agaryes
43230Nutrient agar (NA)yes

culture temp

@refgrowthtypetemperaturerange
43230positiveoptimum25mesophilic
43230positivegrowth15-30

culture pH

@refabilitytypepHPH range
43230positivegrowth6.0-10.0alkaliphile
43230positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 43230
  • oxygen tolerance: aerobe

spore formation

  • @ref: 43230
  • spore description: smooth-surfaced spores on ISP2 medium
  • type of spore: spore
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
43230NaClpositivegrowth0-7 %(w/v)
43230NaClpositiveoptimum3 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
43230casein+degradation
43230chitin+degradation17029
43230esculin+degradation4853
43230xanthine+degradation15318
43230hypoxanthine+degradation17368
43230starch+degradation28017
43230tyrosine+degradation18186
43230cellulose+degradation62968
43230xylan+degradation37166
43230uric acid+degradation27226
43230tween 80+degradation53426
43230adenine-degradation16708
43230urea-degradation16199
43230D-arabinose+carbon source17108
43230dextrin-carbon source23652
43230D-galactose+carbon source12936
43230maltose+carbon source17306
43230L-rhamnose+carbon source62345
43230sorbitol+carbon source30911
43230xylitol+carbon source17151
43230L-arabinose-carbon source30849
43230lactose-carbon source17716
43230D-mannose-carbon source16024
43230raffinose-carbon source16634
43230D-ribose-carbon source16988
43230D-xylose-carbon source65327
43230D-arabinose+builds acid from17108
43230cellobiose+builds acid from17057
43230D-fructose+builds acid from15824
43230D-galactose+builds acid from12936
43230maltose+builds acid from17306
43230D-rhamnose-builds acid from63150
43230D-ribose+builds acid from16988
43230xylose+builds acid from18222
43230L-asparagine+nitrogen source17196
43230L-histidine+nitrogen source15971
43230L-lysine+nitrogen source18019
43230L-glutamate-nitrogen source29985
43230L-serine+nitrogen source17115
43230L-valine+nitrogen source16414
43230tyrosine+carbon source18186

enzymes

@refvalueactivityec
43230acid phosphatase+3.1.3.2
43230alkaline phosphatase+3.1.3.1
43230naphthol-AS-BI-phosphohydrolase+
43230beta-galactosidase+3.2.1.23
43230chymotrypsin+3.4.4.5
43230alpha-glucosidase+3.2.1.20
43230beta-glucosidase+3.2.1.21
43230cystine arylamidase+3.4.11.3
43230esterase (C 4)+
43230esterase Lipase (C 8)+
43230leucine arylamidase+3.4.11.1
43230N-acetyl-beta-glucosaminidase+3.2.1.52
43230trypsin+3.4.21.4
43230alpha-fucosidase-3.2.1.51
43230alpha-galactosidase-3.2.1.22
43230alpha-mannosidase-3.2.1.24
43230beta-glucosidase-3.2.1.21
43230lipase (C 14)-

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43230C16:0 iso26.24
    43230C15:0 iso20.11
    43230C17:0 iso13.26
    4323010-methyl C16:0 / iso-C17:1ω9c11.29
    43230C17:0 anteiso10.65
  • type of FA analysis: whole cell analysis
  • incubation medium: TSA
  • agar/liquid: agar
  • incubation temperature: 28
  • incubation time: 3
  • library/peak naming table: ACTINO
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

  • @ref: 43230
  • sample type: marine sponge Scopalina ruetzleri
  • sampling date: 2013-08
  • geographic location: Saint Peter and Saint Paul archipelago in the Atlantic Ocean, Brazil
  • country: Brazil
  • origin.country: BRA
  • continent: Middle and South America
  • longitude: -29

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Host#Invertebrates (Other)#Porifera (Sponges)

Sequence information

16S sequences

  • @ref: 43230
  • description: 16S rRNA gene sequences
  • accession: KX037095
  • length: 1486
  • database: ena
  • NCBI tax ID: 1847949

GC content

  • @ref: 43230
  • GC-content: 64.8
  • method: Thermal denaturation, fluorometry

External links

@ref: 43230

culture collection no.: DSM 103218, NRRL B-65384, CMAA 1452

literature

  • topic: Phylogeny
  • Pubmed-ID: 28632117
  • title: Saccharopolyspora spongiae sp. nov., a novel actinomycete isolated from the marine sponge Scopalina ruetzleri (Wiedenmayer, 1977).
  • authors: Souza DT, Silva FSPD, Silva LJD, Crevelin EJ, Moraes LAB, Zucchi TD, Melo IS
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001912
  • year: 2017
  • mesh: Animals, Bacterial Typing Techniques, Base Composition, Brazil, DNA, Bacterial/genetics, Nucleic Acid Hybridization, *Phylogeny, Porifera/*microbiology, RNA, Ribosomal, 16S/genetics, Saccharopolyspora/*classification/genetics/isolation & purification, Sequence Analysis, DNA
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43230Danilo Tosta Souza, Fábio Sérgio Paulino da Silva, Leonardo José da Silva, Eduardo José Crevelin, Luiz Alberto Beraldo Moraes, Tiago Domingues Zucchi and Itamar Soares MeloSaccharopolyspora spongiae sp. nov., a novel actinomycete isolated from the marine sponge Scopalina ruetzleri (Wiedenmayer, 1977)10.1099/ijsem.0.001912IJSEM 67: 2019-2025 201728632117