Strain identifier

BacDive ID: 140586

Type strain: Yes

Species: Nesterenkonia cremea

Strain Designation: 10C

Strain history: <- SVV Reddy

NCBI tax ID(s): 1882340 (species)

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General

@ref: 43224

BacDive-ID: 140586

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, rod-shaped, colony-forming

description: Nesterenkonia cremea 10C is an aerobe, mesophilic, Gram-positive bacterium that forms circular colonies and was isolated from sediment sample.

NCBI tax id

  • NCBI tax id: 1882340
  • Matching level: species

strain history

  • @ref: 67771
  • history: <- SVV Reddy

doi: 10.13145/bacdive140586.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Micrococcaceae
  • genus: Nesterenkonia
  • species: Nesterenkonia cremea
  • full scientific name: Nesterenkonia cremea Sultanpuram et al. 2017

@ref: 43224

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Micrococcales

family: Micrococcaceae

genus: Nesterenkonia

species: Nesterenkonia cremea

strain designation: 10C

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
43224positive2.1-3.6 µm0.5-0.6 µmrod-shapedno
69480positive100

colony morphology

  • @ref: 43224
  • colony size: 0.5-1.0 mm
  • colony color: cream
  • colony shape: circular
  • incubation period: 3 days
  • medium used: Marine agar

Culture and growth conditions

culture temp

@refgrowthtypetemperaturerange
43224positiveoptimum37mesophilic
43224positivegrowth10-55
67771positivegrowth37mesophilic

culture pH

@refabilitytypepHPH range
43224positivegrowth7.0-9.0alkaliphile
43224positiveoptimum7.5-8.0

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
43224aerobe
67771aerobe

spore formation

@reftype of sporespore formationconfidence
43224endosporeno
69481no100
69480no99.994

halophily

@refsaltgrowthtested relationconcentration
43224NaClpositiveoptimum5-6 %
43224NaClpositivegrowth2-11 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4322417057cellobiose+builds acid from
4322428757fructose+builds acid from
4322415443inulin+builds acid from
4322417234glucose+builds acid from
4322417992sucrose+builds acid from
4322437684mannose+builds acid from
4322417057cellobiose+builds gas from
4322428757fructose+builds gas from
4322415443inulin+builds gas from
4322417234glucose+builds gas from
4322417992sucrose+builds gas from
4322437684mannose+builds gas from
4322428260galactose-carbon source
4322417814salicin-carbon source
4322426546rhamnose-carbon source
4322430911sorbitol-carbon source
4322428053melibiose-carbon source
4322416634raffinose-carbon source
4322427082trehalose-carbon source
4322417716lactose+carbon source
4322417268myo-inositol+carbon source
4322429864mannitol+carbon source
4322418222xylose+carbon source
4322417306maltose+carbon source
4322422599arabinose+carbon source
4322431206ammonium chloride+nitrogen source
4322418186tyrosine+nitrogen source
4322415428glycine+nitrogen source
4322417632nitrate+nitrogen source
4322416199urea+nitrogen source
4322429987glutamate+nitrogen source
4322416301nitrite-nitrogen source
4322435391aspartate-nitrogen source
4322417716lactose-builds acid from
4322417268myo-inositol-builds acid from
4322429864mannitol-builds acid from
4322418222xylose-builds acid from
4322417306maltose-builds acid from
4322422599arabinose-builds acid from
4322417716lactose-builds gas from
4322417268myo-inositol-builds gas from
4322429864mannitol-builds gas from
4322418222xylose-builds gas from
4322417306maltose-builds gas from
4322422599arabinose-builds gas from
4322428017starch-hydrolysis
432245291gelatin+hydrolysis
4322416024D-mannose+carbon source

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
432242637amikacinyesyes30 µg
4322428971ampicillinyesyes10 µg
4322417698chloramphenicolyesyes30 µg
43224100241ciprofloxacinyesyes5 µg
4322448923erythromycinyesyes15 µg
4322417833gentamicinyesyes101 µg
432246104kanamycinyesyes30 µg
43224100147nalidixic acidyesyes30 µg
4322417334penicillinyesyes10 µg
4322417076streptomycinyesyes10 µg
4322427902tetracyclineyesyes30 µg
4322428001vancomycinyesyes30 µg

enzymes

@refvalueactivityec
43224cytochrome oxidase+1.9.3.1
43224catalase+1.11.1.6
43224gelatinase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43224C17:0 anteiso23.9
    43224C16:0 iso12.5
  • type of FA analysis: whole cell analysis
  • incubation medium: Zobell Marine Broth 2216 5 % (w/v) NaCl
  • agar/liquid: liquid
  • incubation temperature: 37
  • incubation pH: 7.00
  • software version: Sherlock 6.0
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
43224sediment sampleLonar soda lake, Buldhana, Maharashtra, IndiaIndiaINDAsia1976
67771From Lonar soda lake in IndiaIndiaINDAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Sediment
#Environmental#Aquatic#Sediment

Sequence information

16S sequences

  • @ref: 43224
  • description: 16S rRNA gene sequences
  • accession: LT601575
  • length: 1441
  • database: ena
  • NCBI tax ID: 1882340

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Nesterenkonia cremea CGMCC 1.15388GCA_014642675contigncbi1882340
66792Nesterenkonia cremea strain CGMCC 1.153881882340.4wgspatric1882340

GC content

  • @ref: 43224
  • GC-content: 68.6
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno94.063yes
gram-positiveyes92.85yes
anaerobicno99.264no
aerobicyes94.636yes
halophileyes89.106no
spore-formingno92.98no
glucose-utilyes89.423no
flagellatedno96.867yes
glucose-fermentno83.026no
thermophileno95.63yes

External links

@ref: 43224

culture collection no.: LMG 29100, KCTC 39636, CGMCC 1.15388

literature

  • topic: Phylogeny
  • Pubmed-ID: 28665262
  • title: Nesterenkonia cremea sp. nov., a bacterium isolated from a soda lake.
  • authors: Sultanpuram VR, Mothe T, Chintalapati S, Chintalapati VR
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001876
  • year: 2017
  • mesh: *Alkalies, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Fatty Acids/chemistry, India, Lakes/*microbiology, Micrococcaceae/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43224Vishnuvardhan Reddy Sultanpuram, Thirumala Mothe, Sasikala Chintalapati and Venkata Ramana ChintalapatiNesterenkonia cremea sp. nov., a bacterium isolated from a soda lake10.1099/ijsem.0.001876IJSEM 67: 1861-1866 201728665262
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1