Strain identifier

BacDive ID: 140576

Type strain: Yes

Species: Microbulbifer aestuariivivens

Strain Designation: GHTF-23

NCBI tax ID(s): 1908308 (species)

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General

@ref: 43194

BacDive-ID: 140576

keywords: 16S sequence, Bacteria, aerobe, thermophilic, Gram-negative, rod-shaped, colony-forming

description: Microbulbifer aestuariivivens GHTF-23 is an aerobe, thermophilic, Gram-negative bacterium that forms circular colonies and was isolated from Tidal flat sediment.

NCBI tax id

  • NCBI tax id: 1908308
  • Matching level: species

doi: 10.13145/bacdive140576.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Cellvibrionales
  • family: Microbulbiferaceae
  • genus: Microbulbifer
  • species: Microbulbifer aestuariivivens
  • full scientific name: Microbulbifer aestuariivivens Park et al. 2017

@ref: 43194

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Cellvibrionales

family: Microbulbiferaceae

genus: Microbulbifer

species: Microbulbifer aestuariivivens

strain designation: GHTF-23

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
43194negative0.5-10.0 µm0.2-0.5 µmrod-shapedno
43194coccus-shaped

colony morphology

  • @ref: 43194
  • colony size: 1.0-2.0 mm
  • colony color: light yellowish brown
  • colony shape: circular
  • incubation period: 2 days
  • medium used: MA

Culture and growth conditions

culture medium

  • @ref: 43194
  • name: Marine agar (MA)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
43194nogrowth50thermophilic
43194nogrowth10psychrophilic
43194positivegrowth15-45
43194positiveoptimum37mesophilic

culture pH

@refabilitytypepH
43194positiveoptimum6.5-7.5
43194positivegrowth4.5
43194nogrowth4

Physiology and metabolism

oxygen tolerance

  • @ref: 43194
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentration
43194NaClpositiveoptimum2 %(w/v)
43194NaClpositivegrowth0.5-5.0 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4319418420magnesium(2+)+required for growth
4319417632nitrate+reduction
431944853esculin+hydrolysis
431945291gelatin+hydrolysis
4319453426tween 80+hydrolysis
43194casein-hydrolysis
4319417368hypoxanthine-hydrolysis
4319428017starch-hydrolysis
4319417895L-tyrosine-hydrolysis
4319416199urea-hydrolysis
4319415318xanthine-hydrolysis
4319417057cellobiose+builds acid from
4319417634D-glucose+builds acid from
4319430849L-arabinose-builds acid from
4319415824D-fructose-builds acid from
4319412936D-galactose-builds acid from
4319417716lactose-builds acid from
4319417306maltose-builds acid from
4319416024D-mannose-builds acid from
431946731melezitose-builds acid from
4319428053melibiose-builds acid from
4319416634raffinose-builds acid from
4319462345L-rhamnose-builds acid from
4319416988D-ribose-builds acid from
4319417992sucrose-builds acid from
4319427082trehalose-builds acid from
4319465327D-xylose-builds acid from
4319417268myo-inositol-builds acid from
4319416899D-mannitol-builds acid from
4319417924D-sorbitol-builds acid from

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.is sensitivesensitivity conc.
4319428971ampicillinyesyes10 µg (disc)
431943393carbenicillinyesyes100 µg (disc)
431943542cephalothinyesyes30 µg (disc)
4319417698chloramphenicolyesyes100 µg (disc)
4319417833gentamicinyesyes30 µg (disc)
431946104kanamycinyesyes30 µg (disc)
431946472lincomycinyesyes15 µg (disc)
431947507neomycinyesyes30 µg (disc)
4319428368novobiocinyesyes5 µg (disc)
4319416869oleandomycinyesyes15 µg (disc)
4319418208penicillin gyesyes20 Unit
431948309polymyxin byesyes100 Unit
4319417076streptomycinyesyes50 µg (disc)
4319427902tetracyclineyesyes30 µg (disc)

enzymes

@refvalueactivityec
43194catalase+1.11.1.6
43194cytochrome oxidase+1.9.3.1
43194alkaline phosphatase+3.1.3.1
43194esterase (C 4)+
43194esterase Lipase (C 8)+
43194leucine arylamidase+3.4.11.1
43194valine arylamidase+
43194cystine arylamidase+3.4.11.3
43194trypsin-3.4.21.4
43194alpha-chymotrypsin-3.4.21.1
43194acid phosphatase-3.1.3.2
43194naphthol-AS-BI-phosphohydrolase-
43194alpha-galactosidase-3.2.1.22
43194beta-galactosidase-3.2.1.23
43194beta-glucuronidase-3.2.1.31
43194alpha-glucosidase-3.2.1.20
43194beta-glucosidase-3.2.1.21
43194N-acetyl-beta-glucosaminidase-3.2.1.52
43194alpha-mannosidase-3.2.1.24
43194alpha-fucosidase-3.2.1.51

fatty acid profile

  • @reffatty acidpercentage
    43194C10:00.7
    43194C14:02.7
    43194C16:08.5
    43194C17:1ω8c1.1
    43194C18:1ω7c10.8
    43194C11:0 iso3.2
    43194C15:0 iso32.6
    43194C17:0 iso8.9
    43194C15:1 iso F0.7
    43194C17:1 iso ω9c6.2
    43194C10:0 3OH1
    43194C11:0 iso 3OH6.3
    43194C16:1ω7c / C16:1ω6c14.4
  • type of FA analysis: whole cell analysis
  • incubation medium: MA
  • agar/liquid: agar
  • incubation temperature: 37
  • incubation time: 1
  • software version: Sherlock 6.2B
  • system: MIS MIDI
  • @reffatty acidpercentageECL
    43194C10:00.9
    43194C14:03
    43194C16:011.2
    43194C18:00.6
    43194C18:1ω7c8.3
    43194C11:0 iso4.3
    43194C15:0 iso31.8
    43194C17:0 iso7.9
    43194C15:1 iso F1.1
    43194C17:1 iso ω9c6
    43194C10:0 3OH1.2
    43194C11:0 iso 3OH8.2
    43194C16:1ω7c / C16:1ω6c12.2
    43194unknown fatty acid C19:1ω6c / cyclo-C19:0&om0.618846
  • type of FA analysis: whole cell analysis
  • incubation medium: MA
  • agar/liquid: agar
  • incubation temperature: 37
  • incubation time: 2
  • software version: Sherlock 6.2B
  • system: MIS MIDI
  • @reffatty acidpercentageECL
    43194C10:01.1
    43194C14:02.6
    43194C16:011.6
    43194C17:1ω8c0.6
    43194C18:1ω7c8.3
    43194C11:0 iso4.6
    43194C15:0 iso29.3
    43194C17:0 iso7.7
    43194C17:1 iso ω9c6.3
    43194C10:0 3OH1.4
    43194C11:0 iso 3OH9
    43194C16:1ω7c / C16:1ω6c12.9
    43194unknown fatty acid C19:1ω6c / cyclo-C19:0&om0.718846
  • type of FA analysis: whole cell analysis
  • incubation medium: MA
  • agar/liquid: agar
  • incubation temperature: 37
  • incubation time: 3
  • software version: Sherlock 6.2B
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

  • @ref: 43194
  • sample type: Tidal flat sediment
  • geographic location: Goheung on the South Sea
  • country: Republic of Korea
  • origin.country: KOR
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Tidal flat
#Environmental#Terrestrial#Sediment

taxonmaps

  • @ref: 69479
  • File name: preview.99_5911.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_1255;97_3475;98_4392;99_5911&stattab=map
  • Last taxonomy: Microbulbifer
  • 16S sequence: KX982847
  • Sequence Identity:
  • Total samples: 116
  • soil counts: 3
  • aquatic counts: 112
  • animal counts: 1

Sequence information

16S sequences

  • @ref: 43194
  • description: 16S rRNA gene sequences
  • accession: KX982847
  • length: 1458
  • database: ena
  • NCBI tax ID: 1908308

GC content

  • @ref: 43194
  • GC-content: 60.1
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 43194

culture collection no.: NBRC 112533, KCTC 52569

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny28141501Microbulbifer aestuariivivens sp. nov., isolated from a tidal flat.Park S, Yoon SY, Ha MJ, Yoon JHInt J Syst Evol Microbiol10.1099/ijsem.0.0018312017Alteromonadaceae/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Glycolipids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/chemistryTranscriptome
Phylogeny30777816Microbulbifer flavimaris sp. nov., a halophilic Gammaproteobacteria isolated from marine sediment of the Yellow Sea, China.Xiong Q, Wang D, Dong X, Liu D, Liu Y, Li P, Wu G, Luo Y, Zhang R, Liu S, Zhang GInt J Syst Evol Microbiol10.1099/ijsem.0.0032792019Alteromonadaceae/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gammaproteobacteria, Geologic Sediments/*microbiology, Glycolipids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/chemistryTranscriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43194Sooyeon Park, Sun Young Yoon, Min-Ji Ha and Jung-Hoon YoonMicrobulbifer aestuariivivens sp. nov., isolated from a tidal flat10.1099/ijsem.0.001831IJSEM 67: 1436-1441 201728141501
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/