Strain identifier
BacDive ID: 140565
Type strain:
Species: Halorubrum trueperi
Strain Designation: Y73
Strain history: S. Chen; Dept. of Biol., Sch. of Life Sci. & Technol., Honghe Univ., China; Y73.
NCBI tax ID(s): 2004704 (species)
General
@ref: 43186
BacDive-ID: 140565
keywords: 16S sequence, Archaea, aerobe, thermophilic, Gram-negative, pleomorphic-shaped, colony-forming
description: Halorubrum trueperi Y73 is an aerobe, thermophilic, Gram-negative archaeon that forms circular colonies and was isolated from salt mine.
NCBI tax id
- NCBI tax id: 2004704
- Matching level: species
strain history
- @ref: 67770
- history: S. Chen; Dept. of Biol., Sch. of Life Sci. & Technol., Honghe Univ., China; Y73.
doi: 10.13145/bacdive140565.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Halobacteria
- order: Haloferacales
- family: Halorubraceae
- genus: Halorubrum
- species: Halorubrum trueperi
- full scientific name: Halorubrum trueperi Chen et al. 2017
@ref: 43186
domain: Archaea
phylum: Euryarchaeota
class: Halobacteria
order: Haloferacales
family: Halorubraceae
genus: Halorubrum
species: Halorubrum trueperi
strain designation: Y73
type strain: yes
Morphology
cell morphology
- @ref: 43186
- gram stain: negative
- cell length: 2.9-4.1 µm
- cell width: 0.5-0.7 µm
- cell shape: pleomorphic-shaped
- motility: no
colony morphology
- @ref: 43186
- colony size: 2-3 mm
- colony color: orange
- colony shape: circular
- incubation period: 10 days
- medium used: AS-168 medium
Culture and growth conditions
culture medium
- @ref: 43186
- name: AS-168 medium
- growth: yes
- composition: (g/L): yeast extract (Difco) 5, Casamino acids (Difco) 5, NaCl 200, MgSO4 * 7 H2O 20, sodium citrate 3, KCl 2, monosodium glutamate 1.8, FeSO4 * 7 H2O 0.036, MnCl2 * 4 H2O 0.0036
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
43186 | positive | optimum | 42-45 | thermophilic |
43186 | positive | growth | 25-50 | |
67770 | positive | growth | 37 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
43186 | positive | growth | 6.0-9.0 | alkaliphile |
43186 | positive | optimum | 7.5-8.5 |
Physiology and metabolism
oxygen tolerance
- @ref: 43186
- oxygen tolerance: aerobe
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
43186 | NaCl | positive | growth | 15-30 %(w/v) |
43186 | NaCl | positive | optimum | 20 %(w/v) |
43186 | MgCl2 | positive | growth | 0.005-0.3 M |
43186 | MgCl2 | positive | optimum | 0.05 M |
observation
- @ref: 43186
- observation: lyse in distilled water
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
43186 | 17632 | nitrate | - | reduction |
43186 | 16301 | nitrite | - | reduction |
43186 | 28017 | starch | - | hydrolysis |
43186 | 5291 | gelatin | - | hydrolysis |
43186 | casein | - | hydrolysis | |
43186 | 53426 | tween 80 | - | hydrolysis |
43186 | 53424 | tween 20 | + | hydrolysis |
43186 | 53423 | tween 40 | + | hydrolysis |
43186 | 53425 | tween 60 | + | hydrolysis |
43186 | 17634 | D-glucose | + | carbon source |
43186 | 16024 | D-mannose | + | carbon source |
43186 | 12936 | D-galactose | + | carbon source |
43186 | 15824 | D-fructose | + | carbon source |
43186 | 65327 | D-xylose | + | carbon source |
43186 | 17306 | maltose | + | carbon source |
43186 | 17992 | sucrose | + | carbon source |
43186 | 28017 | starch | + | carbon source |
43186 | 17754 | glycerol | + | carbon source |
43186 | 15361 | pyruvate | + | carbon source |
43186 | 24996 | lactate | + | carbon source |
43186 | 30031 | succinate | + | carbon source |
43186 | 25115 | malate | + | carbon source |
43186 | 29806 | fumarate | + | carbon source |
43186 | 29985 | L-glutamate | + | carbon source |
43186 | 30089 | acetate | - | carbon source |
43186 | 17317 | D-sorbose | - | carbon source |
43186 | 16988 | D-ribose | - | carbon source |
43186 | 17716 | lactose | - | carbon source |
43186 | 16899 | D-mannitol | - | carbon source |
43186 | 17924 | D-sorbitol | - | carbon source |
43186 | 16947 | citrate | - | carbon source |
43186 | 15428 | glycine | - | carbon source |
43186 | 16977 | L-alanine | - | carbon source |
43186 | 16467 | L-arginine | - | carbon source |
43186 | 29991 | L-aspartate | - | carbon source |
43186 | 18019 | L-lysine | - | carbon source |
43186 | 15729 | L-ornithine | - | carbon source |
43186 | 17634 | D-glucose | + | builds acid from |
43186 | 16024 | D-mannose | + | builds acid from |
43186 | 15824 | D-fructose | + | builds acid from |
43186 | 65327 | D-xylose | + | builds acid from |
antibiotic resistance
@ref | ChEBI | metabolite | is antibiotic | is resistant | resistance conc. | is sensitive | sensitivity conc. |
---|---|---|---|---|---|---|---|
43186 | 28971 | ampicillin | yes | yes | 10 µg (disc) | ||
43186 | 28669 | bacitracin | yes | yes | 0.04 Unit | ||
43186 | 17698 | chloramphenicol | yes | yes | 30 µg (disc) | ||
43186 | 100241 | ciprofloxacin | yes | yes | 5 µg (disc) | ||
43186 | 48923 | erythromycin | yes | yes | 15 µg (disc) | ||
43186 | 6104 | kanamycin | yes | yes | 10 µg (disc) | ||
43186 | 7507 | neomycin | yes | yes | 30 µg (disc) | ||
43186 | 100246 | norfloxacin | yes | yes | 10 µg (disc) | ||
43186 | 28368 | novobiocin | yes | yes | 30 µg (disc) | ||
43186 | 18208 | penicillin g | yes | yes | 10 Unit | ||
43186 | 28077 | rifampicin | yes | yes | 5 µg (disc) | ||
43186 | 17076 | streptomycin | yes | yes | 10 µg (disc) | ||
43186 | 27902 | tetracycline | yes | yes | 30 µg (disc) | ||
43186 | 28001 | vancomycin | yes | yes | 30 µg (disc) |
metabolite production
- @ref: 43186
- Chebi-ID: 16136
- metabolite: hydrogen sulfide
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
43186 | catalase | + | 1.11.1.6 |
43186 | cytochrome oxidase | - | 1.9.3.1 |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | latitude | longitude |
---|---|---|---|---|---|---|---|
43186 | salt mine | Yuanyonjing Salt Mine, Yunnan province, China | China | CHN | Asia | 25 | 101 |
67770 | Salt mine in Yunnan | China | CHN | Asia |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Condition | #Saline | |
#Engineered | #Other | #Mine |
Sequence information
16S sequences
- @ref: 43186
- description: 16S rRNA sequences
- accession: KX376707
- length: 1459
- database: ena
- NCBI tax ID: 2004704
GC content
- @ref: 67770
- GC-content: 61.9
- method: thermal denaturation, midpoint method (Tm)
External links
@ref: 43186
culture collection no.: CGMCC 1.15503, JCM 31271
literature
- topic: Phylogeny
- Pubmed-ID: 28032536
- title: Halorubrum trueperi sp. nov., a halophilic archaeon isolated from a salt mine.
- authors: Chen S, Xu Y, Ke LX
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.001762
- year: 2017
- mesh: Base Composition, China, DNA, Archaeal/genetics, Glycolipids/chemistry, Halorubrum/*classification/genetics/isolation & purification, *Mining, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride
- topic2: Transcriptome
Reference
@id | authors | title | doi/url | journal | pubmed |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
43186 | Shaoxing Chen, Yao Xu and Li-Xia Ke | Halorubrum trueperi sp. nov., a halophilic archaeon isolated from a salt mine | 10.1099/ijsem.0.001762 | IJSEM 67: 1564-1570 2017 | 28032536 |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ |