Strain identifier

BacDive ID: 140544

Type strain: Yes

Species: Mesorhizobium helmanticense

Strain Designation: CSLC115

NCBI tax ID(s): 1776423 (species)

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General

@ref: 43332

BacDive-ID: 140544

keywords: 16S sequence, Bacteria, aerobe, chemoorganotroph, mesophilic, Gram-negative, motile, rod-shaped, colony-forming

description: Mesorhizobium helmanticense CSLC115 is an aerobe, chemoorganotroph, mesophilic bacterium that forms circular colonies and was isolated from effective nodules ol Lotus corniculatus plants growing in a soil, Carbajosa de la Sagrada, Salamanca, Spain.

NCBI tax id

  • NCBI tax id: 1776423
  • Matching level: species

doi: 10.13145/bacdive140544.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Phyllobacteriaceae
  • genus: Mesorhizobium
  • species: Mesorhizobium helmanticense
  • full scientific name: Mesorhizobium helmanticense Marcos-García et al. 2017

@ref: 43332

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Hyphomicrobiales

family: Phyllobacteriaceae

genus: Mesorhizobium

species: Mesorhizobium helmanticense

strain designation: CSLC115

type strain: yes

Morphology

cell morphology

  • @ref: 43332
  • gram stain: negative
  • cell length: 1.5-3.0 µm
  • cell width: 0.3-0.5 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 43332
  • colony size: 1-2 mm
  • colony color: white
  • colony shape: circular
  • incubation period: 4-5 days
  • medium used: YMA

Culture and growth conditions

culture medium

  • @ref: 43332
  • name: YMA
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
43332positiveoptimum28mesophilic
43332positivegrowth5-35

culture pH

@refabilitytypepHPH range
43332positivegrowth5-9alkaliphile
43332positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 43332
  • oxygen tolerance: aerobe

nutrition type

  • @ref: 43332
  • type: chemoorganotroph

spore formation

  • @ref: 43332
  • spore formation: no

halophily

  • @ref: 43332
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 1 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4333217632nitrate-reduction
433324853esculin+hydrolysis
4333229864mannitol+builds acid from
4333217234glucose+carbon source
4333230849L-arabinose+carbon source
4333262345L-rhamnose+carbon source
4333216988D-ribose+carbon source
4333216024D-mannose+carbon source
4333229864mannitol+carbon source
43332506227N-acetylglucosamine+carbon source
4333217268myo-inositol+carbon source
4333217306maltose+carbon source
4333217992sucrose+carbon source
4333217814salicin+carbon source
4333228053melibiose+carbon source
4333218287L-fucose+carbon source
4333217924D-sorbitol+carbon source
4333225115malate+carbon source
4333215971L-histidine+carbon source
4333217203L-proline+carbon source
4333224265gluconate-carbon source
4333227689decanoate-carbon source
4333217128adipate-carbon source
4333216947citrate-carbon source
4333218401phenylacetate-carbon source
4333217240itaconate-carbon source
433329300suberic acid-carbon source
4333262983sodium malonate-carbon source
4333217272propionate-carbon source
4333231011valerate-carbon source
43332168082-dehydro-D-gluconate-carbon source
43332161933-hydroxybenzoate-carbon source
43332178794-hydroxybenzoate-carbon source
43332370543-hydroxybutyrate-carbon source
4333217115L-serine-carbon source
4333230089acetate+/-carbon source
4333216004(R)-lactate+/-carbon source
4333216651(S)-lactate+/-carbon source
4333228087glycogen+/-carbon source
43332581435-dehydro-D-gluconate+/-carbon source

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.is sensitivesensitivity conc.
4333228971ampicillinyesyes2 µg (disc)
433323515cefuroximeyesyes30 µg (disc)
43332100241ciprofloxacinyesyes5 µg (disc)
4333249566cloxacillinyesyes1 µg (disc)
4333248923erythromycinyesyes2 µg (disc)
4333217833gentamicinyesyes10 µg (disc)
433327507neomycinyesyes5 µg (disc)
4333217334penicillinyesyes10 Unit (disc)
433328309polymyxin byesyes300 Unit (disc)
4333227902tetracyclineyesyes30 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
4333235581indoleno
4333216136hydrogen sulfideno

enzymes

@refvalueactivityec
43332catalase+1.11.1.6
43332cytochrome oxidase+1.9.3.1
43332arginine dihydrolase-3.5.3.6
43332gelatinase-
43332urease+3.5.1.5
43332alpha-glucosidase+3.2.1.20
43332beta-glucosidase+3.2.1.21
43332beta-galactosidase+3.2.1.23
43332alpha-maltosidase+
43332alkaline phosphatase+3.1.3.1
43332alpha-arabinosidase-3.2.1.55
43332beta-L-arabinosidase-3.2.1.88
43332alpha-fucosidase-3.2.1.51
43332beta-D-fucosidase-3.2.1.38
43332alpha-galactosidase-3.2.1.22
43332lactosidase-
43332beta-maltosidase-
43332beta-cellobiase-
43332alpha-mannosidase-3.2.1.24
43332beta-mannosidase-3.2.1.25
43332alpha-L-rhamnosidase-3.2.1.40
43332alpha-xylosidase-
43332beta-xylosidase-
43332galacturonidase-
43332glucuronidase-
43332acid phosphatase-3.1.3.2

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43332C16:012.9
    43332C18:02.2
    43332C12:0 2OH0.5
    43332C12:0 3OH0.6
    43332C13:0 iso 3OH1.7
    43332C17:0 iso5.9
    43332C20:2ω6,9c3.2
    43332C16:1ω7c / C16:1ω6c1.9
    43332C18:1ω7c / C18:1ω6c42.8
    43332C19:0 10-methyl1.6
    43332C18:1ω7c 11-methyl17.8
    43332C19:0 cyclo ω8c7.1
  • type of FA analysis: whole cell analysis
  • incubation medium: TY
  • agar/liquid: agar
  • incubation temperature: 28
  • incubation time: 2
  • software version: Sherlock 6.1
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

  • @ref: 43332
  • sample type: effective nodules ol Lotus corniculatus plants growing in a soil, Carbajosa de la Sagrada, Salamanca, Spain
  • geographic location: Carbajosa de la Sagrada, Salamanca
  • country: Spain
  • origin.country: ESP
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Root nodule

taxonmaps

  • @ref: 69479
  • File name: preview.99_1684.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_220;97_239;98_268;99_1684&stattab=map
  • Last taxonomy: Mesorhizobium
  • 16S sequence: KT899885
  • Sequence Identity:
  • Total samples: 4459
  • soil counts: 2635
  • aquatic counts: 507
  • animal counts: 282
  • plant counts: 1035

Sequence information

16S sequences

  • @ref: 43332
  • description: 16S rRNA gene sequence
  • accession: KT899885
  • database: nuccore

GC content

  • @ref: 43332
  • GC-content: 62.7
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 43332

culture collection no.: CECT 9168, LMG 29734

literature

  • topic: Phylogeny
  • Pubmed-ID: 28693668
  • title: Mesorhizobium helmanticense sp. nov., isolated from Lotus corniculatus nodules.
  • authors: Marcos-Garcia M, Menendez E, Ramirez-Bahena MH, Mateos PF, Peix A, Velazquez E, Rivas R
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001942
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Lotus/*microbiology, Mesorhizobium/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Root Nodules, Plant/*microbiology, Sequence Analysis, DNA, Spain, Symbiosis
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43332Marta Marcos-García, Esther Menéndez, Marta Helena Ramírez-Bahena, Pedro F. Mateos, Álvaro Peix, Encarna Velazquez, Raúl RivasMesorhizobium helmanticense sp. nov., isolated from Lotus corniculatus nodules10.1099/ijsem.0.001942IJSEM 67: 2301-2305 201728693668
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/