Strain identifier

BacDive ID: 140541

Type strain: Yes

Species: Bradyrhizobium cajani

Strain Designation: AMBPC1011

NCBI tax ID(s): 1928661 (species)

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General

@ref: 43328

BacDive-ID: 140541

keywords: 16S sequence, Bacteria, aerobe, Gram-negative, rod-shaped

description: Bradyrhizobium cajani AMBPC1011 is an aerobe, Gram-negative, rod-shaped bacterium that was isolated from effective root nodules of plants from C. cajan cultivated at Monte Monito in Azúa, Dominican Republic.

NCBI tax id

  • NCBI tax id: 1928661
  • Matching level: species

doi: 10.13145/bacdive140541.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Nitrobacteraceae
  • genus: Bradyrhizobium
  • species: Bradyrhizobium cajani
  • full scientific name: Bradyrhizobium cajani Araújo et al. 2017

@ref: 43328

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Hyphomicrobiales

family: Bradyrhizobiaceae

genus: Bradyrhizobium

species: Bradyrhizobium cajani

strain designation: AMBPC1011

type strain: yes

Morphology

cell morphology

  • @ref: 43328
  • gram stain: negative
  • cell shape: rod-shaped

colony morphology

  • @ref: 43328
  • colony color: pearl-white
  • medium used: YMA

Culture and growth conditions

culture medium

  • @ref: 43328
  • name: YMA
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
43328positivegrowth15-37
43328positiveoptimum28mesophilic

culture pH

@refabilitytypepHPH range
43328positiveoptimum7-7.5
43328positivegrowth5-9alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 43328
  • oxygen tolerance: aerobe

halophily

  • @ref: 43328
  • salt: NaCl
  • growth: no
  • tested relation: growth
  • concentration: 1 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4332817632nitrate+reduction
4332816199urea+hydrolysis
433284853esculin+hydrolysis
4332817234glucose-fermentation
4332837684mannose+builds acid from
4332825115malate+builds acid from
4332817128adipate+builds acid from
4332816947citrate+builds acid from
4332818401phenylacetate+builds acid from
4332824265gluconate-builds acid from
4332862345L-rhamnose+builds acid from
43328506227N-acetylglucosamine+builds acid from
4332816988D-ribose+builds acid from
433289300suberic acid+builds acid from
43328581435-dehydro-D-gluconate+builds acid from
43328168082-dehydro-D-gluconate+builds acid from
4332828087glycogen+builds acid from
4332829864mannitol+builds acid from
4332817234glucose+builds acid from
4332818287L-fucose+builds acid from
4332817203L-proline+builds acid from
4332817268myo-inositol-builds acid from
4332817992sucrose-builds acid from
4332817240itaconate-builds acid from
4332862983sodium malonate-builds acid from
4332816411indole-3-acetic acid-builds acid from
43328L-alanine 4-nitroanilide-builds acid from
43328161933-hydroxybenzoate-builds acid from
4332817115L-serine-builds acid from
4332817814salicin-builds acid from
4332828053melibiose-builds acid from
4332817266L-sorbose-builds acid from
4332830849L-arabinose-builds acid from
4332817272propionate-builds acid from
4332827689decanoate-builds acid from
43328370543-hydroxybutyrate-builds acid from
4332817306maltose+/-builds acid from
4332816004(R)-lactate+/-builds acid from
4332816651(S)-lactate+/-builds acid from
4332831011valerate+/-builds acid from
4332816947citrate+/-builds acid from
4332815971L-histidine+/-builds acid from

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.is sensitivesensitivity conc.
4332828971ampicillinyesyes2 µg (disc)
433283515cefuroximeyesyes30 µg (disc)
4332849566cloxacillinyesyes1 µg (disc)
4332848923erythromycinyesyes2 µg (disc)
4332817833gentamicinyesyes10 µg (disc)
433287507neomycinyesyes5 µg (disc)
4332817334penicillinyesyes10 Unit (disc)
433288309polymyxin byesyes300 Unit (disc)
4332827902tetracyclineyesyes30 µg (disc)

metabolite production

  • @ref: 43328
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
43328beta-galactosidase+/-3.2.1.23
43328arginine dihydrolase-3.5.3.6
43328gelatinase-

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43328C16:011.4
    43328C18:02
    43328C16:1ω5c1.3
    43328C18:1ω5c1
    43328C16:1ω7c / C16:1ω6c0.4
    43328C18:1ω7c / C18:1ω6c83.4
    43328C18:1ω7c 11-methyl0.6
  • type of FA analysis: whole cell analysis
  • incubation medium: YMA
  • agar/liquid: agar
  • incubation temperature: 28
  • incubation time: 2
  • incubation_oxygen: aerobic
  • software version: Sherlock 6.1
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

  • @ref: 43328
  • sample type: effective root nodules of plants from C. cajan cultivated at Monte Monito in Azúa, Dominican Republic
  • geographic location: Monte Monito, Azúa
  • country: Dominican Republic
  • origin.country: DOM
  • continent: Middle and South America
  • enrichment culture: YMA agar

isolation source categories

Cat1Cat2Cat3
#Host#Plants
#Host Body-Site#Plant#Root nodule

taxonmaps

  • @ref: 69479
  • File name: preview.99_871.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_86;96_297;97_329;98_370;99_871&stattab=map
  • Last taxonomy: Bradyrhizobium
  • 16S sequence: KY349447
  • Sequence Identity:
  • Total samples: 5154
  • soil counts: 1424
  • aquatic counts: 688
  • animal counts: 2839
  • plant counts: 203

Sequence information

16S sequences

  • @ref: 43328
  • description: 16S rRNA
  • accession: KY349447
  • database: nuccore

GC content

  • @ref: 43328
  • GC-content: 62.7
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 43328

culture collection no.: CECT 9227, LMG 29967

literature

  • topic: Phylogeny
  • Pubmed-ID: 28671523
  • title: Bradyrhizobium cajani sp. nov. isolated from nodules of Cajanus cajan.
  • authors: Araujo J, Flores-Felix JD, Igual JM, Peix A, Gonzalez-Andres F, Diaz-Alcantara CA, Velazquez E
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001932
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, Bradyrhizobium/*classification/genetics/isolation & purification, Cajanus/*microbiology, DNA, Bacterial/genetics, Dominican Republic, Genes, Bacterial, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Root Nodules, Plant/*microbiology, Sequence Analysis, DNA, Symbiosis
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43328Juan Araújo, José David Flores-Félix, José M. Igual, Alvaro Peix, Fernando González-Andrés, César Antonio Díaz-Alcántara, Encarna VelázquezBradyrhizobium cajani sp. nov. isolated from nodules of Cajanus cajan10.1099/ijsem.0.001932IJSEM 67: 2236-2241 201728671523
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/