Strain identifier

BacDive ID: 140521

Type strain: Yes

Species: Paenibacillus arachidis

Strain Designation: E3

Strain history: <- Sadaf Kalam, Univ. of Hyderabad

NCBI tax ID(s): 1500780 (species)

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General

@ref: 43081

BacDive-ID: 140521

keywords: 16S sequence, Bacteria, facultative anaerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped

description: Paenibacillus arachidis E3 is a facultative anaerobe, spore-forming, mesophilic bacterium that was isolated from groundnut seed.

NCBI tax id

  • NCBI tax id: 1500780
  • Matching level: species

strain history

  • @ref: 67771
  • history: <- Sadaf Kalam, Univ. of Hyderabad

doi: 10.13145/bacdive140521.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Paenibacillus
  • species: Paenibacillus arachidis
  • full scientific name: Paenibacillus arachidis Sadaf et al. 2016

@ref: 43081

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Paenibacillaceae

genus: Paenibacillus

species: Paenibacillus arachidis

strain designation: E3

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
43081positive1.4-1.7 µm0.5-0.7 µmrod-shapedyes
67771rod-shapedyes
67771positive

colony morphology

  • @ref: 43081
  • colony color: white
  • medium used: LB

Culture and growth conditions

culture medium

  • @ref: 43081
  • name: LB (Luria-Bertani) MEDIUM
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
43081positiveoptimum35mesophilic
43081positivegrowth30-40
67771positivegrowth37mesophilic

culture pH

@refabilitytypepH
43081positivegrowth6.5-8.0
43081positiveoptimum7.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
43081facultative anaerobe
67771aerobe

spore formation

  • @ref: 43081
  • spore description: endospore
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
43081NaClpositivegrowth0-2 %
43081NaClpositiveoptimum2 %

observation

  • @ref: 43081
  • observation: biofilm formation

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
430815291gelatin+hydrolysis
4308116199urea+hydrolysis
4308128017starch+hydrolysis
4308117234glucose-fermentation
4308116947citrate-carbon source
4308115824D-fructose+growth
4308116988D-ribose+growth
4308117992sucrose+growth
4308128017starch+growth
43081506227N-acetylglucosamine+growth
4308117634D-glucose+growth
4308150144sodium pyruvate+growth
4308117924D-sorbitol+growth
4308162345L-rhamnose-growth
4308117268myo-inositol-growth
4308116899D-mannitol-growth
4308117306maltose-growth
4308118300maleic acid-growth
4308127082trehalose+growth

metabolite production

@refChebi-IDmetaboliteproduction
4308126672siderophoreyes
4308115688acetoinno
4308135581indoleno

metabolite tests

@refChebi-IDmetabolitemethylred-testvoges-proskauer-testcitrate test
4308117234glucose-
4308115688acetoin-
4308116947citrate-

enzymes

@refvalueactivityec
43081catalase+1.11.1.6
43081chitinase+3.2.1.14
43081esterase+
43081acid phosphatase+3.1.3.2
43081naphthol-AS-BI-phosphohydrolase+
43081beta-galactosidase+3.2.1.23
43081alpha-glucosidase+3.2.1.20
43081N-acetyl-beta-glucosaminidase+3.2.1.52
43081alkaline phosphatase-3.1.3.1
43081esterase Lipase (C 8)-
43081lipase (C 14)-
43081leucine arylamidase-3.4.11.1
43081valine arylamidase-
43081cystine arylamidase-3.4.11.3
43081trypsin-3.4.21.4
43081alpha-chymotrypsin-3.4.21.1
43081alpha-galactosidase-3.2.1.22
43081beta-glucuronidase-3.2.1.31
43081beta-glucosidase-3.2.1.21
43081alpha-mannosidase-3.2.1.24
43081alpha-fucosidase-3.2.1.51

Isolation, sampling and environmental information

isolation

@refsample typeenrichment cultureenrichment culture durationenrichment culture temperaturegeographic locationcountryorigin.countrycontinent
43081groundnut seedLuria Bertani (LB) agar5 days37
67771From groundnut seedsHyderabadIndiaINDAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Greenhouse
#Host Body-Site#Plant#Fruit (Seed)

taxonmaps

  • @ref: 69479
  • File name: preview.99_124944.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15358;96_48959;97_63006;98_83931;99_124944&stattab=map
  • Last taxonomy: Paenibacillus arachidis subclade
  • 16S sequence: KJ572789
  • Sequence Identity:
  • Total samples: 14
  • soil counts: 9
  • animal counts: 2
  • plant counts: 3

Sequence information

16S sequences

  • @ref: 43081
  • description: Paenibacillus arachidis strain E3 16S ribosomal RNA gene, partial sequence
  • accession: KJ572789
  • length: 1404
  • database: nuccore
  • NCBI tax ID: 1500780

GC content

@refGC-contentmethod
4308153high performance liquid chromatography (HPLC)
6777153.0

External links

@ref: 43081

culture collection no.: KCTC 33574, LMG 28417

literature

  • topic: Phylogeny
  • Pubmed-ID: 27129367
  • title: Paenibacillus arachidis sp. nov., isolated from groundnut seeds.
  • authors: Sadaf K, Tushar L, Nirosha P, Podile AR, Sasikala C, Ramana CV
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001124
  • year: 2016
  • mesh: Arachis/*microbiology, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Paenibacillus/*classification/genetics/isolation & purification, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seeds/*microbiology, Sequence Analysis, DNA
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43081K. Sadaf, L. Tushar, P. Nirosha, A. R. Podile, Ch. Sasikala, Ch. V. RamanaPaenibacillus arachidis sp. nov., isolated from groundnut seeds10.1099/ijsem.0.001124IJSEM 66: 2923-2928 201627129367
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/