Strain identifier
BacDive ID: 140493
Type strain:
Species: Streptomyces rhizosphaerihabitans
Strain Designation: JR-35, IMER00654
NCBI tax ID(s): 1266770 (species)
General
@ref: 67668
BacDive-ID: 140493
DSM-Number: 111589
keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive
description: Streptomyces rhizosphaerihabitans JR-35 is an aerobe, spore-forming, mesophilic bacterium that builds a substrate mycelium and was isolated from bamboo rhizosphere soil.
NCBI tax id
- NCBI tax id: 1266770
- Matching level: species
doi: 10.13145/bacdive140493.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Kitasatosporales
- family: Streptomycetaceae
- genus: Streptomyces
- species: Streptomyces rhizosphaerihabitans
- full scientific name: Streptomyces rhizosphaerihabitans Lee and Whang 2016
@ref: 67668
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Streptomycetales
family: Streptomycetaceae
genus: Streptomyces
species: Streptomyces rhizosphaerihabitans
full scientific name: Streptomyces rhizosphaerihabitans Lee and Whang 2016
strain designation: JR-35, IMER00654
type strain: yes
Morphology
cell morphology
- @ref: 43108
- gram stain: positive
- motility: no
multicellular morphology
@ref | forms multicellular complex | complex name | further description |
---|---|---|---|
43108 | yes | substrate mycelium | extensively branched |
43108 | yes | aerial hyphae | differentiate into straight to flexuous (Rectiflexibiles) chains of hairy spores |
pigmentation
- @ref: 43108
- production: yes
- name: Melanin pigments (on ISP 6 medium)
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
43108 | ISP 2 | yes | ||
43108 | ISP 3 | yes | ||
43108 | ISP 4 | yes | ||
43108 | ISP 5 | yes | ||
43108 | ISP 6 | yes | ||
43108 | ISP 7 | yes | ||
67668 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
67668 | STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) | yes | https://mediadive.dsmz.de/medium/252 | Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
43108 | positive | optimum | 28 | mesophilic |
43108 | positive | growth | 4-37 | |
67668 | positive | growth | 28 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
43108 | positive | growth | 4.0-9.0 | alkaliphile |
43108 | positive | optimum | 6.0-8.0 |
Physiology and metabolism
oxygen tolerance
- @ref: 43108
- oxygen tolerance: aerobe
spore formation
- @ref: 43108
- spore formation: yes
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
43108 | NaCl | positive | growth | 0-5 %(w/v) |
43108 | NaCl | no | growth | 7 %(w/v) |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
43108 | 17632 | nitrate | + | reduction |
43108 | casein | + | hydrolysis | |
43108 | 62968 | cellulose | + | hydrolysis |
43108 | 5291 | gelatin | - | hydrolysis |
43108 | 28017 | starch | - | hydrolysis |
43108 | 16199 | urea | - | hydrolysis |
43108 | 17634 | D-glucose | + | carbon source |
43108 | 15824 | D-fructose | + | carbon source |
43108 | 16899 | D-mannitol | + | carbon source |
43108 | 16634 | raffinose | + | carbon source |
43108 | 62345 | L-rhamnose | + | carbon source |
43108 | 17992 | sucrose | + | carbon source |
43108 | 30849 | L-arabinose | + | carbon source |
43108 | 65327 | D-xylose | + | carbon source |
fatty acid profile
fatty acids
@ref fatty acid percentage 43108 C15:0 anteiso 29.15 43108 C16:0 iso 19.66 43108 C14:0 iso 13.36 43108 C15:0 iso 12.02 - type of FA analysis: whole cell analysis
- incubation medium: tryptic soy broth (TSB, Difco)
- agar/liquid: liquid
- incubation temperature: 28
- incubation time: 5
- software version: Sherlock 4.5
- system: MIS MIDI
- cutoff value:
Isolation, sampling and environmental information
isolation
- @ref: 43108
- sample type: bamboo (Sasa borealis) rhizosphere soil
- geographic location: Damyang, Jeonnam
- country: Republic of Korea
- origin.country: KOR
- continent: Asia
- enrichment culture: starch casein agar
- enrichment culture duration: 7 days
- enrichment culture temperature: 28
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Terrestrial | #Soil |
#Host | #Plants | #Herbaceous plants (Grass,Crops) |
#Host Body-Site | #Plant | #Rhizosphere |
Safety information
risk assessment
- @ref: 67668
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 43108
- description: Streptomyces sp. JR-35 16S ribosomal RNA gene, partial sequence
- accession: HQ267983
- length: 1462
- database: nuccore
- NCBI tax ID: 1266770
GC content
- @ref: 67668
- GC-content: 69.4
- method: high performance liquid chromatography (HPLC)
External links
@ref: 67668
culture collection no.: KACC 17181, NBRC 109807, DSM 111589
straininfo link
- @ref: 96731
- straininfo: 397621
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 27298302 | Streptomyces rhizosphaerihabitans sp. nov. and Streptomyces adustus sp. nov., isolated from bamboo forest soil. | Lee HJ, Whang KS | Int J Syst Evol Microbiol | 10.1099/ijsem.0.001236 | 2016 | Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Forests, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sasa/*microbiology, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
Phylogeny | 29995193 | Streptomyces lutosisoli sp. nov., a novel actinomycete isolated from muddy soil. | Shen Y, Sun T, Jiang S, Mu S, Li D, Guo X, Zhang J, Zhao J, Xiang W | Antonie Van Leeuwenhoek | 10.1007/s10482-018-1129-2 | 2018 | Bacterial Typing Techniques, China, DNA, Bacterial/genetics, Fatty Acids/chemistry/metabolism, Phylogeny, RNA, Ribosomal, 16S/genetics, Soil/chemistry, *Soil Microbiology, Streptomyces/classification/genetics/*isolation & purification/metabolism | Metabolism |
Phylogeny | 31948045 | Taxonomic Characterization, and Secondary Metabolite Analysis of Streptomyces triticiradicis sp. nov.: A Novel Actinomycete with Antifungal Activity. | Yu Z, Han C, Yu B, Zhao J, Yan Y, Huang S, Liu C, Xiang W | Microorganisms | 10.3390/microorganisms8010077 | 2020 |
Reference
@id | authors | title | doi/url | journal | pubmed | catalogue |
---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
43108 | Hyo-Jin Lee, Kyung-Sook Whang | Streptomyces rhizosphaerihabitans sp. nov. and Streptomyces adustus sp. nov., isolated from bamboo forest soil | 10.1099/ijsem.0.001236 | IJSEM 66: 3573-3578 2016 | 27298302 | |
67668 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-111589 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 111589) | |||
96731 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID397621.1 |