Strain identifier

BacDive ID: 140493

Type strain: Yes

Species: Streptomyces rhizosphaerihabitans

Strain Designation: JR-35, IMER00654

NCBI tax ID(s): 1266770 (species)

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General

@ref: 67668

BacDive-ID: 140493

DSM-Number: 111589

keywords: 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Streptomyces rhizosphaerihabitans JR-35 is an aerobe, spore-forming, mesophilic bacterium that builds a substrate mycelium and was isolated from bamboo rhizosphere soil.

NCBI tax id

  • NCBI tax id: 1266770
  • Matching level: species

doi: 10.13145/bacdive140493.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces rhizosphaerihabitans
  • full scientific name: Streptomyces rhizosphaerihabitans Lee and Whang 2016

@ref: 67668

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Streptomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces rhizosphaerihabitans

full scientific name: Streptomyces rhizosphaerihabitans Lee and Whang 2016

strain designation: JR-35, IMER00654

type strain: yes

Morphology

cell morphology

  • @ref: 43108
  • gram stain: positive
  • motility: no

multicellular morphology

@refforms multicellular complexcomplex namefurther description
43108yessubstrate myceliumextensively branched
43108yesaerial hyphaedifferentiate into straight to flexuous (Rectiflexibiles) chains of hairy spores

pigmentation

  • @ref: 43108
  • production: yes
  • name: Melanin pigments (on ISP 6 medium)

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43108ISP 2yes
43108ISP 3yes
43108ISP 4yes
43108ISP 5yes
43108ISP 6yes
43108ISP 7yes
67668GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
67668STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
43108positiveoptimum28mesophilic
43108positivegrowth4-37
67668positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
43108positivegrowth4.0-9.0alkaliphile
43108positiveoptimum6.0-8.0

Physiology and metabolism

oxygen tolerance

  • @ref: 43108
  • oxygen tolerance: aerobe

spore formation

  • @ref: 43108
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
43108NaClpositivegrowth0-5 %(w/v)
43108NaClnogrowth7 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4310817632nitrate+reduction
43108casein+hydrolysis
4310862968cellulose+hydrolysis
431085291gelatin-hydrolysis
4310828017starch-hydrolysis
4310816199urea-hydrolysis
4310817634D-glucose+carbon source
4310815824D-fructose+carbon source
4310816899D-mannitol+carbon source
4310816634raffinose+carbon source
4310862345L-rhamnose+carbon source
4310817992sucrose+carbon source
4310830849L-arabinose+carbon source
4310865327D-xylose+carbon source

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43108C15:0 anteiso29.15
    43108C16:0 iso19.66
    43108C14:0 iso13.36
    43108C15:0 iso12.02
  • type of FA analysis: whole cell analysis
  • incubation medium: tryptic soy broth (TSB, Difco)
  • agar/liquid: liquid
  • incubation temperature: 28
  • incubation time: 5
  • software version: Sherlock 4.5
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

  • @ref: 43108
  • sample type: bamboo (Sasa borealis) rhizosphere soil
  • geographic location: Damyang, Jeonnam
  • country: Republic of Korea
  • origin.country: KOR
  • continent: Asia
  • enrichment culture: starch casein agar
  • enrichment culture duration: 7 days
  • enrichment culture temperature: 28

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Rhizosphere

Safety information

risk assessment

  • @ref: 67668
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 43108
  • description: Streptomyces sp. JR-35 16S ribosomal RNA gene, partial sequence
  • accession: HQ267983
  • length: 1462
  • database: nuccore
  • NCBI tax ID: 1266770

GC content

  • @ref: 67668
  • GC-content: 69.4
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 67668

culture collection no.: KACC 17181, NBRC 109807, DSM 111589

straininfo link

  • @ref: 96731
  • straininfo: 397621

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny27298302Streptomyces rhizosphaerihabitans sp. nov. and Streptomyces adustus sp. nov., isolated from bamboo forest soil.Lee HJ, Whang KSInt J Syst Evol Microbiol10.1099/ijsem.0.0012362016Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Forests, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sasa/*microbiology, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny29995193Streptomyces lutosisoli sp. nov., a novel actinomycete isolated from muddy soil.Shen Y, Sun T, Jiang S, Mu S, Li D, Guo X, Zhang J, Zhao J, Xiang WAntonie Van Leeuwenhoek10.1007/s10482-018-1129-22018Bacterial Typing Techniques, China, DNA, Bacterial/genetics, Fatty Acids/chemistry/metabolism, Phylogeny, RNA, Ribosomal, 16S/genetics, Soil/chemistry, *Soil Microbiology, Streptomyces/classification/genetics/*isolation & purification/metabolismMetabolism
Phylogeny31948045Taxonomic Characterization, and Secondary Metabolite Analysis of Streptomyces triticiradicis sp. nov.: A Novel Actinomycete with Antifungal Activity.Yu Z, Han C, Yu B, Zhao J, Yan Y, Huang S, Liu C, Xiang WMicroorganisms10.3390/microorganisms80100772020

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43108Hyo-Jin Lee, Kyung-Sook WhangStreptomyces rhizosphaerihabitans sp. nov. and Streptomyces adustus sp. nov., isolated from bamboo forest soil10.1099/ijsem.0.001236IJSEM 66: 3573-3578 201627298302
67668Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-111589Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 111589)
96731Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID397621.1